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The map label for this gene is metF [H]

Identifier: 169834245

GI number: 169834245

Start: 630946

End: 631812

Strand: Direct

Name: metF [H]

Synonym: SPH_0685

Alternate gene names: 169834245

Gene position: 630946-631812 (Clockwise)

Preceding gene: 169833651

Following gene: 169833588

Centisome position: 28.1

GC content: 43.48

Gene sequence:

>867_bases
ATGTCACGCCAAACACCGTCACTCTCATTTGAAGTGTTCCCTCCAAACCCAGCCGTGGGTAATGGTAACATTATTTCAGC
CTTGCAGGATATGCAGGAGTTGGCTCCCCATTTTATCAGTGTAACTGCCAGCAATAATAAATTTAATATCAAGGAAACGA
CGGTTCGTTTGGCTGACTTTATCCAAAATGATTTGGCGATTCCGACCATTGCCCACTTGCCAGCTATTTATCTAACTAAG
GACAAGGTTGCTGAAACTATTGCTGACTTGGACAAAGTTGGGGTGCAGAAAATCTTGGCTCTTCGTGGGGATATTATTCC
AGACGTGGAACCACAAAAGGATTTCCGCTATGCAACCGACTTGATTGAGTTTATCAAGGAACAAACCCCTCACTTTGATA
TTATTGGAGCTTGCTATCCAGAAGGGCATCCAGATTCGCCAAATCAGATTTCAGATATTCAAAATCTTAAGAAGAAAGTG
GATGCAGGCTGTTCGAGCCTCGTAACTCAGCTCTTCTTTGACAATGAGCGCTTCTATGATTTTCAGGACAAGTGCATCTT
GGCTGGGATTGATGTTCCCATTCATGCAGGAATTATGCCAATTCTGAATCGAAATCAGGCTCTCCGACTCTTGAAGACTT
GTGAGAATATCCATCTTCCACGCAAATTTAAAGCCATCTTAGACAAGTATGAGCATGACCCTGAATCGCTCAGAGCAGCA
GGACTTGCCTATGCAGTGGACCAAATCGTGGACTTGGTAACTCAGGATGTTGCCGGTGTGCATCTCTATACGATGAATAA
TGCTGATACAGCAAAATACATCCATCAAGCAACCCATGCCTTGTTTAATCACCAGTCTCTAGGATAA

Upstream 100 bases:

>100_bases
TGTTGAAGCAGCTAAAGCTGCGCGTGAGAAATTGTAAGATTGGATTTCTCTCCATACAGTGGTTGTTCAAAAAGAAATCA
ACTAGAAAAGGTTATTACAT

Downstream 100 bases:

>100_bases
TAAAAGCAAACCATTCTTCTCAGGTGAGGGGAATGGTTCCTTTTTAATGGTAAAGACCTCACTTTTTAAGAAAAATATGA
TAAAATAAATTCTGTACGTA

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTASNNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTK
DKVAETIADLDKVGVQKILALRGDIIPDVEPQKDFRYATDLIEFIKEQTPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIHLPRKFKAILDKYEHDPESLRAA
GLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQATHALFNHQSLG

Sequences:

>Translated_288_residues
MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTASNNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTK
DKVAETIADLDKVGVQKILALRGDIIPDVEPQKDFRYATDLIEFIKEQTPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIHLPRKFKAILDKYEHDPESLRAA
GLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQATHALFNHQSLG
>Mature_287_residues
SRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTASNNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKD
KVAETIADLDKVGVQKILALRGDIIPDVEPQKDFRYATDLIEFIKEQTPHFDIIGACYPEGHPDSPNQISDIQNLKKKVD
AGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIHLPRKFKAILDKYEHDPESLRAAG
LAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQATHALFNHQSLG

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=266, Percent_Identity=32.3308270676692, Blast_Score=136, Evalue=2e-32,
Organism=Escherichia coli, GI1790377, Length=268, Percent_Identity=33.955223880597, Blast_Score=154, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI25147436, Length=274, Percent_Identity=29.5620437956204, Blast_Score=124, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI25147441, Length=274, Percent_Identity=29.5620437956204, Blast_Score=124, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6321313, Length=276, Percent_Identity=32.6086956521739, Blast_Score=122, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6325234, Length=289, Percent_Identity=30.4498269896194, Blast_Score=116, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24662658, Length=261, Percent_Identity=24.904214559387, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 32115; Mature: 31984

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTASNNKFNIKETTVRLADF
CCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCEEEHHHHHHHHHHH
IQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALRGDIIPDVEPQKDFRYATD
HHHCCCCCHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
LIEFIKEQTPHFDIIGACYPEGHPDSPNQISDIQNLKKKVDAGCSSLVTQLFFDNERFYD
HHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
FQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIHLPRKFKAILDKYEHDPESLRAA
CCCCEEEEECCCCHHCCCHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHH
GLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQATHALFNHQSLG
HHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTASNNKFNIKETTVRLADF
CCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCEEEHHHHHHHHHHH
IQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALRGDIIPDVEPQKDFRYATD
HHHCCCCCHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
LIEFIKEQTPHFDIIGACYPEGHPDSPNQISDIQNLKKKVDAGCSSLVTQLFFDNERFYD
HHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
FQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIHLPRKFKAILDKYEHDPESLRAA
CCCCEEEEECCCCHHCCCHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHH
GLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQATHALFNHQSLG
HHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]