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The map label for this gene is xth [H]

Identifier: 169833563

GI number: 169833563

Start: 1821389

End: 1822216

Strand: Direct

Name: xth [H]

Synonym: SPH_1959

Alternate gene names: 169833563

Gene position: 1821389-1822216 (Clockwise)

Preceding gene: 169833397

Following gene: 169833722

Centisome position: 81.11

GC content: 45.05

Gene sequence:

>828_bases
ATGAAACTTATCTCATGGAATATTGATTCCCTAAACGCTGCCCTAACTAGTGACTCAGCTCGTGCCAAATTGTCCCAAGA
AGTCCTACAAACCTTGGTCGCTGAAAATGCTGATATCATTGCTATCCAAGAAACCAAGCTTTCTGCCAAAGGACCTACAA
AGAAACACTTAGAAATTTTAGAAGAACTCTTCCCAGGCTACGAAAACACGTGGCGTTCTTCCCAAGAGCCTGCCCGTAAA
GGCTATGCTGGAACCATGTTCCTTTATAAAAAAGAACTTACACCTACTATCAGCTTCCCAGAAATCGGTGCCCCTTCTAC
CATGGACTTGGAAGGCCGTATCATCACTCTAGAATTTGATGCATTTTTCGTAACCCAAGTTTACACTCCAAACGCTGGTG
ACGGTCTCAAACGCTTGGAAGAACGCCAAGTCTGGGATGTTAAATATGCTGAGTATTTGGCTGAACTAGACAAAGAAAAA
CCAGTCCTTGCGACCGGTGACTACAACGTAGCCCACAATGAAATCGACCTTGCAAATCCTGCTAGCAACCGCCGTTCACC
TGGATTTACTGACGAGGAACGTGCTGGATTTACCAACCTTTTGGCAACTGGATTTACAGACACCTTCCGCCATGTTCATG
GCGATGTTCCTGAACGCTACACTTGGTGGGCACAACGCAGCAAAACTTCTAAAATCAACAATACAGGCTGGAGAATCGAC
TACTGGCTCACAAGTAACCGTATCGCTGACAAGGTCACTAAATCTGATATGATTGACTCAGGTGCTCGCCAAGACCATAC
ACCGATTGTCTTGGAAATTGACTTGTAA

Upstream 100 bases:

>100_bases
ATTATAAACGAAAGAGAGTAATTTTTCAAACCATACGTTGGGGAAATAGATCTTTTTTTGGTATAATAAAATCTATAATC
TGAATGAAAAAGGTAACTTT

Downstream 100 bases:

>100_bases
GAATTTTCTATATGGACTACAATGCGGTCATTCCCGAGTTGCTTGTATCGAATATCGAACAGTCCCGGTCCTTCTACTGT
GGTTTACTGGGTTTTAGGAT

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQETKLSAKGPTKKHLEILEELFPGYENTWRSSQEPARK
GYAGTMFLYKKELTPTISFPEIGAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPERYTWWAQRSKTSKINNTGWRID
YWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL

Sequences:

>Translated_275_residues
MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQETKLSAKGPTKKHLEILEELFPGYENTWRSSQEPARK
GYAGTMFLYKKELTPTISFPEIGAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPERYTWWAQRSKTSKINNTGWRID
YWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL
>Mature_275_residues
MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQETKLSAKGPTKKHLEILEELFPGYENTWRSSQEPARK
GYAGTMFLYKKELTPTISFPEIGAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPERYTWWAQRSKTSKINNTGWRID
YWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL

Specific function: In addition to 3'- to 5'-exonuclease and 3'-phosphatase activities, ExoA was shown to make single-strand breaks at apurinic sites in DNA [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=277, Percent_Identity=37.9061371841155, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI18375503, Length=277, Percent_Identity=37.9061371841155, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI18375501, Length=277, Percent_Identity=37.9061371841155, Blast_Score=172, Evalue=4e-43,
Organism=Escherichia coli, GI1788046, Length=298, Percent_Identity=27.8523489932886, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71989536, Length=275, Percent_Identity=39.2727272727273, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI71989539, Length=162, Percent_Identity=32.0987654320988, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI221330655, Length=273, Percent_Identity=36.6300366300366, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI17136678, Length=273, Percent_Identity=36.6300366300366, Blast_Score=166, Evalue=2e-41,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 31106; Mature: 31106

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00727 AP_NUCLEASE_F1_2 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQETKLSAKGPTKKHLEIL
CEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHH
EELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEIGAPSTMDLEGRIITLEFD
HHHCCCCCHHCCCCCCHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCEECCCEEEEEEEC
AFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEKPVLATGDYNVAHNEIDLANP
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEECCCC
ASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPERYTWWAQRSKTSKINNTGWRID
CCCCCCCCCCCHHHCCHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCEEE
YWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL
EEEECCHHHHHHHHHHHHCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQETKLSAKGPTKKHLEIL
CEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHH
EELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEIGAPSTMDLEGRIITLEFD
HHHCCCCCHHCCCCCCHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCEECCCEEEEEEEC
AFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEKPVLATGDYNVAHNEIDLANP
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEECCCC
ASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPERYTWWAQRSKTSKINNTGWRID
CCCCCCCCCCCHHHCCHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCEEE
YWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL
EEEECCHHHHHHHHHHHHCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]