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The map label for this gene is proS [H]

Identifier: 169833273

GI number: 169833273

Start: 326596

End: 328449

Strand: Direct

Name: proS [H]

Synonym: SPH_0381

Alternate gene names: 169833273

Gene position: 326596-328449 (Clockwise)

Preceding gene: 169832703

Following gene: 169833865

Centisome position: 14.54

GC content: 43.04

Gene sequence:

>1854_bases
ATGAAACAAAGTAAAATGCCTATCCCAACGCTTCGCGAAATGCCAAGCGATGCTCAAGTTATCAGCCATGCTCTTATGTT
GCGTGCTGGTTATGTTCGCCAAGTTTCAGCAGGTGTTTATTCTTATCTACCACTTGCCAACCGTGTGATTGAAAAAGCTA
AAAACATCATGCGCCAAGAATTCGAAAAGATTGGTGCTGTTGAGATGTTGGCTCCTGCCCTTCTTAGTGCAGAATTGTGG
CGTGAATCAGGTCGTTACGAAACCTATGGTGAAGACCTTTACAAACTAAAAAACCGTGAAAAATCAGACTTTATCTTAGG
TCCAACTCATGAAGAAACCTTTACAGCTATTGTCCGTGATTCTGTTAAATCTTACAAGCAATTGCCACTCAACCTTTATC
AAATTCAGCCCAAGTATCGTGATGAAAAACGCCCACGTAATGGACTTCTTCGTACACGTGAGTTTATCATGAAGGATGCT
TATAGTTTCCACGCTAACTATGATAGTTTGGATAGTGTTTATGATGAGTACAAAGCAGCCTATGAGCGTATTTTCACTCG
TAGTGGTTTAGACTTCAAGGCTATTATTGGTGACGGTGGAGCCATGGGTGGTAAGGATAGCCAAGAATTTATGGCCATTA
CATCTGCTCGTACAGACCTTGACCGCTGGGTTGTCTTGGACAAGTCAGTTGCCTCATTTGACGAAATTCCTGCAGAAGTG
CAAGAAGAAATCAAGGCAGAATTGCTCAAATGGATAGTCTCTGGTGAAGATACCATTGCTTACTCAAGTGAGTCTAGCTA
TGCAGCTAACTTAGAAATGGCAACAAACGAGTACAAACCAAGCAACCGTGTTGTCGCTGAAGAAGAAGTTACTCGTGTTG
CAACGCCAGATGTTAAATCAATTGATGAAGTTGCAGCCTTCCTCAATGTTCCAGAAGAACAAACGATTAAAACCCTCTTC
TACATTGCAGATGGCGAGCTTGTTGCAGCCCTTCTAGTTGGAAATGACCAACTTAACGAAGTCAAGTTGAAAAATCACTT
GGGAGCAGATTTCTTTGACGTTGCTAGCGAAGAAGAAGTGGCGAATGTTGTTCAAGCAGGATTTGGTTCACTTGGACCAG
TTGGTTTGCCAGAGAATATTAAAATTATTGCAGATCGTAAGGTACAAGATGTTCGCAATGCAGTTGTCGGTGCTAACGAA
GATGGCTACCACTTGACTGGTGTGAACCCAGGCCGTGATTTTACTGCAGAATATGTGGATATCCGTGAAGTTCGTGAGGG
TGAAATTTCCCCAGATGGACAAGGTGTCCTTAACTTTGCGCGTGGTATTGAGATCGGTCATATTTTCAAACTCGGAACTC
GCTATTCAGCAAGCATGGGAGCAGATGTCTTGGATGAAAATGGTCGTGCTGTGCCAATCATCATGGGATGTTACGGTATC
GGTGTCAGCCGTCTTCTTTCAGCAGTGATGGAGCAACACGCTCGCCTCTTTGTTAACAAAACGCCAAAAGGTGAATACCG
TTACGCTTGGGGAATCAATTTCCCTAAAGAATTGGCACCATTTGATGTGCATTTGATTACTGTCAATGTCAAGGATGAAG
AAGCGCAAGCCTTGACAGAAAAACTTGAAGCAAGCTTGATGGGAGCTGGTTATGAAGTCTTGACAGATGACCGTAACGAA
CGTGTCGGTGTTAAATTTAGCGATAGTGACTTGATTGGTCTTCCAATCCGTATCACTGTTGGTAAAAAAGCGGCCGATGG
CATTGTAGAAGTTAAGATCAAGGCAACTGGTGACACCATTGAGGTTCATGCAGATAACGTGCTTGAAACGCTTGAAATCC
TAAGCAAGAAATAA

Upstream 100 bases:

>100_bases
CTATGTCACCTTGGCCGGAGTGGTCATCATGGTTGTCTTGATGATTGCTGTGACTTGGAATGACATTATGCGACTCTTTT
TTAGATAATCGAGGAATATT

Downstream 100 bases:

>100_bases
AAACTATAATCAGAAGAAAAACAAGGAAAAAATGTAACTAGTTTTTACCTTGTTTTTTCTGTATAATGGGAGAAATGAGT
AGATAAAGAGGTAAATGTAT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 617; Mature: 617

Protein sequence:

>617_residues
MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLPLANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELW
RESGRYETYGEDLYKLKNREKSDFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSARTDLDRWVVLDKSVASFDEIPAEV
QEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATNEYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLF
YIADGELVAALLVGNDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVRNAVVGANE
DGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFKLGTRYSASMGADVLDENGRAVPIIMGCYGI
GVSRLLSAVMEQHARLFVNKTPKGEYRYAWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNE
RVGVKFSDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK

Sequences:

>Translated_617_residues
MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLPLANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELW
RESGRYETYGEDLYKLKNREKSDFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSARTDLDRWVVLDKSVASFDEIPAEV
QEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATNEYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLF
YIADGELVAALLVGNDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVRNAVVGANE
DGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFKLGTRYSASMGADVLDENGRAVPIIMGCYGI
GVSRLLSAVMEQHARLFVNKTPKGEYRYAWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNE
RVGVKFSDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK
>Mature_617_residues
MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLPLANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELW
RESGRYETYGEDLYKLKNREKSDFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSARTDLDRWVVLDKSVASFDEIPAEV
QEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATNEYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLF
YIADGELVAALLVGNDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVRNAVVGANE
DGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFKLGTRYSASMGADVLDENGRAVPIIMGCYGI
GVSRLLSAVMEQHARLFVNKTPKGEYRYAWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNE
RVGVKFSDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=264, Percent_Identity=34.469696969697, Blast_Score=165, Evalue=1e-40,
Organism=Escherichia coli, GI1786392, Length=619, Percent_Identity=42.81098546042, Blast_Score=471, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI115532348, Length=234, Percent_Identity=32.4786324786325, Blast_Score=137, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6320931, Length=199, Percent_Identity=35.1758793969849, Blast_Score=136, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6322112, Length=161, Percent_Identity=26.0869565217391, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322654, Length=158, Percent_Identity=27.2151898734177, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24656200, Length=199, Percent_Identity=34.6733668341709, Blast_Score=141, Evalue=2e-33,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 68596; Mature: 68596

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLPLANRVIEKAKNIMRQE
CCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKSDFILGPTHEETFTAIVRD
HHHHCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHH
SVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDAYSFHANYDSLDSVYDEYKAA
HHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
YERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSARTDLDRWVVLDKSVASFDEIPAEV
HHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCHHEEEECCHHHHHHHCCHHH
QEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATNEYKPSNRVVAEEEVTRVATPDVKS
HHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEECCCCCCCCCEEEHHHHHHCCCCCHHH
IDEVAAFLNVPEEQTIKTLFYIADGELVAALLVGNDQLNEVKLKNHLGADFFDVASEEEV
HHHHHHHHCCCCHHHEEEEEEEECCCEEEEEEECCCCCCHHHHHHHCCCCHHHCCCHHHH
ANVVQAGFGSLGPVGLPENIKIIADRKVQDVRNAVVGANEDGYHLTGVNPGRDFTAEYVD
HHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHH
IREVREGEISPDGQGVLNFARGIEIGHIFKLGTRYSASMGADVLDENGRAVPIIMGCYGI
HHHHHCCCCCCCCCHHHHHHHCCCHHHEEEECCCCCCCCCCHHHCCCCCEEEEEEEHHHH
GVSRLLSAVMEQHARLFVNKTPKGEYRYAWGINFPKELAPFDVHLITVNVKDEEAQALTE
HHHHHHHHHHHHHHHEEEECCCCCCEEEEECCCCCHHCCCCEEEEEEEEECCHHHHHHHH
KLEASLMGAGYEVLTDDRNERVGVKFSDSDLIGLPIRITVGKKAADGIVEVKIKATGDTI
HHHHHHHCCCCEEEECCCCCEEEEEECCCCEEEEEEEEEECCCCCCCEEEEEEEECCCEE
EVHADNVLETLEILSKK
EEEHHHHHHHHHHHHCC
>Mature Secondary Structure
MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLPLANRVIEKAKNIMRQE
CCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKSDFILGPTHEETFTAIVRD
HHHHCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHH
SVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDAYSFHANYDSLDSVYDEYKAA
HHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
YERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSARTDLDRWVVLDKSVASFDEIPAEV
HHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCHHEEEECCHHHHHHHCCHHH
QEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATNEYKPSNRVVAEEEVTRVATPDVKS
HHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEECCCCCCCCCEEEHHHHHHCCCCCHHH
IDEVAAFLNVPEEQTIKTLFYIADGELVAALLVGNDQLNEVKLKNHLGADFFDVASEEEV
HHHHHHHHCCCCHHHEEEEEEEECCCEEEEEEECCCCCCHHHHHHHCCCCHHHCCCHHHH
ANVVQAGFGSLGPVGLPENIKIIADRKVQDVRNAVVGANEDGYHLTGVNPGRDFTAEYVD
HHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHH
IREVREGEISPDGQGVLNFARGIEIGHIFKLGTRYSASMGADVLDENGRAVPIIMGCYGI
HHHHHCCCCCCCCCHHHHHHHCCCHHHEEEECCCCCCCCCCHHHCCCCCEEEEEEEHHHH
GVSRLLSAVMEQHARLFVNKTPKGEYRYAWGINFPKELAPFDVHLITVNVKDEEAQALTE
HHHHHHHHHHHHHHHEEEECCCCCCEEEEECCCCCHHCCCCEEEEEEEEECCHHHHHHHH
KLEASLMGAGYEVLTDDRNERVGVKFSDSDLIGLPIRITVGKKAADGIVEVKIKATGDTI
HHHHHHHCCCCEEEECCCCCEEEEEECCCCEEEEEEEEEECCCCCCCEEEEEEEECCCEE
EVHADNVLETLEILSKK
EEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA