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The map label for this gene is gmuD [H]

Identifier: 169833865

GI number: 169833865

Start: 328548

End: 329927

Strand: Direct

Name: gmuD [H]

Synonym: SPH_0382

Alternate gene names: 169833865

Gene position: 328548-329927 (Clockwise)

Preceding gene: 169833273

Following gene: 169834503

Centisome position: 14.63

GC content: 42.9

Gene sequence:

>1380_bases
ATGCTAAGATTTCCAAAGGATTTTGTCTGGGGATCCTCTACTTCTGGACCGCAGACAGAAGGACGTGTAGCTGGTGACGG
TAAGGGAGACAATCTCTGGGATTACTGGTTCCAAGTGGAGCCAAATCGTTACTATAATGGGATTGGTTCAGATAAGACAT
CGACTTTTTATGAAAATTGGGAGCGGGATATTGAGCTTTTGTTAGAGACTGGTCACACAGCCTTTCGGACTTCTATTCAG
TGGTCACGGATTTTTCCACAAGGCTGTGGAAAAGTCAACACTCAAGGTGTGGATTTTTATCGTAAGGTCTTTGAGGCTAT
TAAGGCTAAAGGAATTCGTCTGTTAGTCAATCTCTATCATTTTGATTTACCTTTTGCCCTTCAAGAGGATGGTGATGGTT
GGGAAAATAAGGCGACAGTCTCAGCCTATGAAGACTATGCTCGTTTTTGTTTTGAGACTTATGGAGATTTAGTGGATCAA
TGGATTACCTTTAACGAGCCCATCGTTCCTGTAGAATTTGGTTATTTTTATGATGCCCATTATCCACATAAGGTGGATGC
AGAGGCAGCTGTTAAGGTAGCCTATCATACACAATTGGCCAGCAGCCGGGCTGTTAAGGCTTGCCATGAACTTTTGCCTG
ATTCCAAGATTGGGATTGTCCTCAACTTGACACCGGCTTATCCACGTAGCCAGCATCCTGCTGATGTCAAGGCAGCTCGT
ATTGCGGCCCTTTTTCAGGCCCAATCTTTCTTAGATCCATCTGTCTTGGGGACTTATCCACAGGAGTTGGTAGAAATCTT
GCATGAACACGGTCTTTTACCTGATGCTACAGAGGAAGAGTTGGAACTCATTCGTGATAATACGGTGGACTTCCTTGGTG
TGAACTACTATCAACCTTTGCGTGTTATGGCTCCTCGATTTGCTAAGCATCCAGAGAGTCCACTCTTACCAGAACATTTT
TACGAGCCTTATGTGATGCCTGGACGTAAAATCAATTCTCACCGTGGTTGGGAGATTTATGAGCAAGGGATTTATGACAT
CGCCCAAAATATCAAGGAAAATTATGGCAATATTGAATGGATGTTGACTGAGAATGGTATGGGTGTTGAAGGGGAAGAAA
AATTCCGTCAAGATGGAATGATTCAAGATGATTACCGTATTGACTTTGTAAAAGGTCATCTTCGTGAACTTCACCGTGCC
ATTGAAGATGGTGCCAACTGTAAGGGCTACTTGATTTGGACCTTTATTGACTGCTGGTCATGGCTCAACAGCTATAAAAA
TCGCTATGGTTTGGTCGAATTAGACTTGGAAACGCAAGAACGTCGTCTGAAGAAATCAGGCCACTGGTTCAAAAAATTAA
GCGATAATAATGGATTTTAA

Upstream 100 bases:

>100_bases
AAAAACTATAATCAGAAGAAAAACAAGGAAAAAATGTAACTAGTTTTTACCTTGTTTTTTCTGTATAATGGGAGAAATGA
GTAGATAAAGAGGTAAATGT

Downstream 100 bases:

>100_bases
AAAGGACTCTGACTGATTATCCCAATCGGTCAGAGTTTTTTGCTTACAGGAGCTTAATTTGAACTATACCAATTTTTACT
CTTGACAAGTGAAAGAAACA

Product: glycosy hydrolase family protein

Products: NA

Alternate protein names: Aryl-phospho-beta-D-glucosidase BglD; Glucomannan utilization protein D [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRYYNGIGSDKTSTFYENWERDIELLLETGHTAFRTSIQ
WSRIFPQGCGKVNTQGVDFYRKVFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIGIVLNLTPAYPRSQHPADVKAAR
IAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDATEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHF
YEPYVMPGRKINSHRGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKGHLRELHRA
IEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWFKKLSDNNGF

Sequences:

>Translated_459_residues
MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRYYNGIGSDKTSTFYENWERDIELLLETGHTAFRTSIQ
WSRIFPQGCGKVNTQGVDFYRKVFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIGIVLNLTPAYPRSQHPADVKAAR
IAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDATEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHF
YEPYVMPGRKINSHRGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKGHLRELHRA
IEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWFKKLSDNNGF
>Mature_459_residues
MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRYYNGIGSDKTSTFYENWERDIELLLETGHTAFRTSIQ
WSRIFPQGCGKVNTQGVDFYRKVFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIGIVLNLTPAYPRSQHPADVKAAR
IAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDATEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHF
YEPYVMPGRKINSHRGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKGHLRELHRA
IEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWFKKLSDNNGF

Specific function: Phospho-beta-D-glucosidase that seems to be involved in the degradation of glucomannan. Is also capable of hydrolyzing aryl-phospho-beta-D-glucosides, although very weakly, and plays only a minor role, if any, in the degradation of these substrates in viv

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI32481206, Length=482, Percent_Identity=31.5352697095436, Blast_Score=198, Evalue=8e-51,
Organism=Homo sapiens, GI110681710, Length=473, Percent_Identity=30.0211416490486, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI13273313, Length=483, Percent_Identity=30.2277432712215, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI28376633, Length=471, Percent_Identity=28.6624203821656, Blast_Score=168, Evalue=8e-42,
Organism=Homo sapiens, GI24497614, Length=481, Percent_Identity=28.0665280665281, Blast_Score=147, Evalue=3e-35,
Organism=Escherichia coli, GI1789070, Length=483, Percent_Identity=33.1262939958592, Blast_Score=222, Evalue=4e-59,
Organism=Escherichia coli, GI2367270, Length=473, Percent_Identity=32.76955602537, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI2367174, Length=488, Percent_Identity=31.1475409836066, Blast_Score=212, Evalue=5e-56,
Organism=Caenorhabditis elegans, GI17539390, Length=466, Percent_Identity=29.8283261802575, Blast_Score=213, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17552856, Length=468, Percent_Identity=29.7008547008547, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI21356577, Length=479, Percent_Identity=30.062630480167, Blast_Score=199, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 53171; Mature: 53171

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRYYNGIGSDKTSTFYENW
CCCCCCHHCCCCCCCCCCCCCEECCCCCCCCCEEEEEEECCCHHCCCCCCCCCHHHHHHH
ERDIELLLETGHTAFRTSIQWSRIFPQGCGKVNTQGVDFYRKVFEAIKAKGIRLLVNLYH
HHHHHHHHHCCCHHHHHCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEHHH
FDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQWITFNEPIVPVEFGYFYDAH
CCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEECCC
YPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIGIVLNLTPAYPRSQHPADVKAAR
CCCCCCHHHHEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHH
IAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDATEEELELIRDNTVDFLGVNYYQPL
HHHHHHHHHHCCCHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCEEEEECCCCCHH
RVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSHRGWEIYEQGIYDIAQNIKENYGNIEW
HHHCCHHHCCCCCCCCHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCEEE
MLTENGMGVEGEEKFRQDGMIQDDYRIDFVKGHLRELHRAIEDGANCKGYLIWTFIDCWS
EEECCCCCCCCHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHH
WLNSYKNRYGLVELDLETQERRLKKSGHWFKKLSDNNGF
HHHHHHHCCCEEEEECCHHHHHHHHCCCHHHHCCCCCCC
>Mature Secondary Structure
MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRYYNGIGSDKTSTFYENW
CCCCCCHHCCCCCCCCCCCCCEECCCCCCCCCEEEEEEECCCHHCCCCCCCCCHHHHHHH
ERDIELLLETGHTAFRTSIQWSRIFPQGCGKVNTQGVDFYRKVFEAIKAKGIRLLVNLYH
HHHHHHHHHCCCHHHHHCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEHHH
FDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQWITFNEPIVPVEFGYFYDAH
CCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEECCC
YPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIGIVLNLTPAYPRSQHPADVKAAR
CCCCCCHHHHEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHH
IAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDATEEELELIRDNTVDFLGVNYYQPL
HHHHHHHHHHCCCHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCEEEEECCCCCHH
RVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSHRGWEIYEQGIYDIAQNIKENYGNIEW
HHHCCHHHCCCCCCCCHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCEEE
MLTENGMGVEGEEKFRQDGMIQDDYRIDFVKGHLRELHRAIEDGANCKGYLIWTFIDCWS
EEECCCCCCCCHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHH
WLNSYKNRYGLVELDLETQERRLKKSGHWFKKLSDNNGF
HHHHHHHCCCEEEEECCHHHHHHHHCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]