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The map label for this gene is cls [C]

Identifier: 169825149

GI number: 169825149

Start: 1600171

End: 1601694

Strand: Direct

Name: cls [C]

Synonym: FMG_1452

Alternate gene names: 169825149

Gene position: 1600171-1601694 (Clockwise)

Preceding gene: 169825148

Following gene: 169825150

Centisome position: 89.02

GC content: 35.56

Gene sequence:

>1524_bases
ATGAAAAAATTATTCAAATTTTTAACTAGTAGAATAATGCTTCTGGCAATTGTATTTATCATTCAAATTTCTCTATTAGT
TTATTTGATTTTAAGTTTTCAAAGAAATTTTGTGTACGTGTACACATTAAACATTATAATATCCTTCGCTTTTACGATAA
TGGTGGTCAACACCGATGTAAACCCGAGTTTCAAACTAGGATGGCTTATTCCTATATGGATATTTCCGTTGTTTGGAGCG
GTGTTCTATTTGTTTTTCAGAAGAAACAGATTCGTTCGTGCACAACAAAGACGAATGGACACGATAAGTTCAACATTTAA
CAACCATATCTTGTCAAATGGTAATGTAATTGACGAGCTTGAAGGAGACTCCAAGTCAATCGCAACTTACATAAACACAA
CAGCTCACGCTCCAGTTTTTTCGAAAACGAGTTCGAAATATTTGAAAAACGGCGAAGTTTTCTTCGAGTATTTGATGGAA
GATTTAAAAAACGCAAAGGATTATATTTTCTTGGAGTTTTTTATAATCGAAGAAGGCTACATGTTCAATAATATTTTGGA
AGTTTTGGAACAAAAAGTAAAAGAAGGAGTCGACGTCAGGATTTTGTACGACGATTTCGGTTGCATGACGAAACTTTCGC
GTAGATATTACAACAAGCTAGAAAAAAAAGGTATAAAAGTTCAAGTTTTCAATCCGATTCGAGTTCTACCAATGCCTCGT
CACAACAACAGAGATCACAGAAAAATCATCGTTATAGATGGAAAAGTCGCTTACACTGGCGGATTTAATTTGGCGGATGA
ATACATGAACAAAATCCAAAAATTTGGATATTGGAAGGACACAGGAATCAGAGTTACAGGTGATGGTGTGTGGTCATTTA
CGATAATGTTCTTGTCAATTTGGGAATTGTACGATGACAATGAGCTTGACTACAAATATTATTTCGAAAAAACTGTCCCA
GATGTTTCCGAAAACGAAGGTTATTGCCAACCTTATACGGATTCTCCATTAGACGATGTTCCTGTTGGAAAGGGTGCGTA
CATGCAATTGGTGTCTCGTTCCAGAAAATATTTCTACATTACAACTCCGTATTTGATTCCATCAGATGACATGATAGATT
CCCTAAGAGATGCGGCGTTAAGCGGTGTCGATGTCAGAATCATGACTCCTGGAATTCCCGACAAAAAATTGGTGTATATG
GTTACAAGAACATACTACAAGTCGCTTTTGGAAGCGGGTGTTAGAATTTTCGAATACTCACCAGGATTTTTGCACGCAAA
AAGTTGTATTAGTGATGACAAATACTGTGTCATTGGCTCTATCAATCTCGATTTTAGATCATTGTATCTACACTTTGAAA
ACGGAGCAATGTACTACGATTCACACATTATTTCCGATTTAAAGGAAGATTTCTTGGAAATGCAATCCCAATCACGAGAA
ATTTTCTCCAGACAATTGGAAAGCCAAAGCGTGTTTAGAAAAATTATATCCGTAATACTAAAAGTATTCTCACCAATGAT
ATGA

Upstream 100 bases:

>100_bases
ATAAAAGAAGAAAACCTCGGCGATTTTGAATTGATTTTCAAAGTCTGCTGAGGTTTTGTGTTATAATTAAGTAAATTTAA
AAATATTTGTGGAGGTGCAA

Downstream 100 bases:

>100_bases
TGAAACGAAATAATCTAATCGATACGTTAAGGGGATTTGCGATTGTAAACATGGTGATTTATCATTTGCTGTTCGATCTT
GTTTACATGGAAAATTTCAA

Product: cardiolipin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MKKLFKFLTSRIMLLAIVFIIQISLLVYLILSFQRNFVYVYTLNIIISFAFTIMVVNTDVNPSFKLGWLIPIWIFPLFGA
VFYLFFRRNRFVRAQQRRMDTISSTFNNHILSNGNVIDELEGDSKSIATYINTTAHAPVFSKTSSKYLKNGEVFFEYLME
DLKNAKDYIFLEFFIIEEGYMFNNILEVLEQKVKEGVDVRILYDDFGCMTKLSRRYYNKLEKKGIKVQVFNPIRVLPMPR
HNNRDHRKIIVIDGKVAYTGGFNLADEYMNKIQKFGYWKDTGIRVTGDGVWSFTIMFLSIWELYDDNELDYKYYFEKTVP
DVSENEGYCQPYTDSPLDDVPVGKGAYMQLVSRSRKYFYITTPYLIPSDDMIDSLRDAALSGVDVRIMTPGIPDKKLVYM
VTRTYYKSLLEAGVRIFEYSPGFLHAKSCISDDKYCVIGSINLDFRSLYLHFENGAMYYDSHIISDLKEDFLEMQSQSRE
IFSRQLESQSVFRKIISVILKVFSPMI

Sequences:

>Translated_507_residues
MKKLFKFLTSRIMLLAIVFIIQISLLVYLILSFQRNFVYVYTLNIIISFAFTIMVVNTDVNPSFKLGWLIPIWIFPLFGA
VFYLFFRRNRFVRAQQRRMDTISSTFNNHILSNGNVIDELEGDSKSIATYINTTAHAPVFSKTSSKYLKNGEVFFEYLME
DLKNAKDYIFLEFFIIEEGYMFNNILEVLEQKVKEGVDVRILYDDFGCMTKLSRRYYNKLEKKGIKVQVFNPIRVLPMPR
HNNRDHRKIIVIDGKVAYTGGFNLADEYMNKIQKFGYWKDTGIRVTGDGVWSFTIMFLSIWELYDDNELDYKYYFEKTVP
DVSENEGYCQPYTDSPLDDVPVGKGAYMQLVSRSRKYFYITTPYLIPSDDMIDSLRDAALSGVDVRIMTPGIPDKKLVYM
VTRTYYKSLLEAGVRIFEYSPGFLHAKSCISDDKYCVIGSINLDFRSLYLHFENGAMYYDSHIISDLKEDFLEMQSQSRE
IFSRQLESQSVFRKIISVILKVFSPMI
>Mature_507_residues
MKKLFKFLTSRIMLLAIVFIIQISLLVYLILSFQRNFVYVYTLNIIISFAFTIMVVNTDVNPSFKLGWLIPIWIFPLFGA
VFYLFFRRNRFVRAQQRRMDTISSTFNNHILSNGNVIDELEGDSKSIATYINTTAHAPVFSKTSSKYLKNGEVFFEYLME
DLKNAKDYIFLEFFIIEEGYMFNNILEVLEQKVKEGVDVRILYDDFGCMTKLSRRYYNKLEKKGIKVQVFNPIRVLPMPR
HNNRDHRKIIVIDGKVAYTGGFNLADEYMNKIQKFGYWKDTGIRVTGDGVWSFTIMFLSIWELYDDNELDYKYYFEKTVP
DVSENEGYCQPYTDSPLDDVPVGKGAYMQLVSRSRKYFYITTPYLIPSDDMIDSLRDAALSGVDVRIMTPGIPDKKLVYM
VTRTYYKSLLEAGVRIFEYSPGFLHAKSCISDDKYCVIGSINLDFRSLYLHFENGAMYYDSHIISDLKEDFLEMQSQSRE
IFSRQLESQSVFRKIISVILKVFSPMI

Specific function: Catalyzes The Reversible Phosphatidyl Group Transfer From One Phosphatidylglycerol Molecule To Another To Form Cardiolipin (Cl) (Diphosphatidylglycerol) And Glycerol. Affects Resistance To The Gyrase Inhibitor Novobiocin. [C]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=475, Percent_Identity=26.5263157894737, Blast_Score=171, Evalue=7e-44,
Organism=Escherichia coli, GI1787007, Length=333, Percent_Identity=27.027027027027, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 59319; Mature: 59319

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLFKFLTSRIMLLAIVFIIQISLLVYLILSFQRNFVYVYTLNIIISFAFTIMVVNTDV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHEEEEEECCC
NPSFKLGWLIPIWIFPLFGAVFYLFFRRNRFVRAQQRRMDTISSTFNNHILSNGNVIDEL
CCCEEEHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCEEECC
EGDSKSIATYINTTAHAPVFSKTSSKYLKNGEVFFEYLMEDLKNAKDYIFLEFFIIEEGY
CCCCHHHHHHHCCCCCCCCCCCCCHHHHHCHHHHHHHHHHHHCCCCCEEEEEEEEEECCC
MFNNILEVLEQKVKEGVDVRILYDDFGCMTKLSRRYYNKLEKKGIKVQVFNPIRVLPMPR
HHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCC
HNNRDHRKIIVIDGKVAYTGGFNLADEYMNKIQKFGYWKDTGIRVTGDGVWSFTIMFLSI
CCCCCCCEEEEEECEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHH
WELYDDNELDYKYYFEKTVPDVSENEGYCQPYTDSPLDDVPVGKGAYMQLVSRSRKYFYI
HHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEE
TTPYLIPSDDMIDSLRDAALSGVDVRIMTPGIPDKKLVYMVTRTYYKSLLEAGVRIFEYS
ECCEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEECC
PGFLHAKSCISDDKYCVIGSINLDFRSLYLHFENGAMYYDSHIISDLKEDFLEMQSQSRE
CCCHHHHHHCCCCCEEEEEECCCCEEEEEEEEECCEEEEHHHHHHHHHHHHHHHHHHHHH
IFSRQLESQSVFRKIISVILKVFSPMI
HHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKLFKFLTSRIMLLAIVFIIQISLLVYLILSFQRNFVYVYTLNIIISFAFTIMVVNTDV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHEEEEEECCC
NPSFKLGWLIPIWIFPLFGAVFYLFFRRNRFVRAQQRRMDTISSTFNNHILSNGNVIDEL
CCCEEEHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCEEECC
EGDSKSIATYINTTAHAPVFSKTSSKYLKNGEVFFEYLMEDLKNAKDYIFLEFFIIEEGY
CCCCHHHHHHHCCCCCCCCCCCCCHHHHHCHHHHHHHHHHHHCCCCCEEEEEEEEEECCC
MFNNILEVLEQKVKEGVDVRILYDDFGCMTKLSRRYYNKLEKKGIKVQVFNPIRVLPMPR
HHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCC
HNNRDHRKIIVIDGKVAYTGGFNLADEYMNKIQKFGYWKDTGIRVTGDGVWSFTIMFLSI
CCCCCCCEEEEEECEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHH
WELYDDNELDYKYYFEKTVPDVSENEGYCQPYTDSPLDDVPVGKGAYMQLVSRSRKYFYI
HHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEE
TTPYLIPSDDMIDSLRDAALSGVDVRIMTPGIPDKKLVYMVTRTYYKSLLEAGVRIFEYS
ECCEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEECC
PGFLHAKSCISDDKYCVIGSINLDFRSLYLHFENGAMYYDSHIISDLKEDFLEMQSQSRE
CCCHHHHHHCCCCCEEEEEECCCCEEEEEEEEECCEEEEHHHHHHHHHHHHHHHHHHHHH
IFSRQLESQSVFRKIISVILKVFSPMI
HHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12397186; 2761387 [H]