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The map label for this gene is ppdK [H]

Identifier: 169824497

GI number: 169824497

Start: 868591

End: 871221

Strand: Direct

Name: ppdK [H]

Synonym: FMG_0800

Alternate gene names: 169824497

Gene position: 868591-871221 (Clockwise)

Preceding gene: 169824496

Following gene: 169824498

Centisome position: 48.32

GC content: 32.99

Gene sequence:

>2631_bases
ATGACAGAAAAATATGTTTACAGTTTTAGAGAAGGTAACAAAGATATGAGAAGCCTTCTTGGTGGAAAAGGTGCTAACTT
AGCCGAAATGACTAACATGGATATTCCTGTTCCTACAGGATTTACTATTACTACCGAAGCTTGTAACAAATTTTACCAAG
AAGATGAAAAATTATGGGATGGATTATTTGAAGAAATTAAATCTCATATTAAAGAAACAGAAAAAGAATTGAATAAGAAA
TTTTCTGATGAAGAAAATCCTCTACTATTTTCAGTTCGTTCAGGTGCTGTAATATCAATGCCTGGTATGATGGACACAAT
TTTAAATCTAGGTTTAAATGATAAATCAGTTATCGGTTTAGCAAAGTCTACAAATAATGAAAGATTTGCTTATGATTCAT
ATAGAAGATTTATTCAAATGTTTTCAGACGTAGCAAGAGAAATTCCTAAAGTATTGTTCGAAAATGAATTAGATAAAATT
AAACAAGAAAAAAACGTTACAGACGATACAGATCTTGATAGTGAAGATTTAAAAAAGATTGTAGAAAAATTTAAACAAAT
CTACAAGGAAGAAATGGATGAAGATTTCCCACAAGACCCTATCGATCAATTAAAATATGCAATCGAAGCAGTTTTTAGAT
CTTGGAATAACCCAAGAGCAAAAATTTACAGACAATTAAATGGCATTGATGATCATTTAGGAACTGCTGTAAATATCCAA
TCAATGGTATTTGGTAACATGGGTAATACATCTGGAACTGGTGTTGCTTTCTCAAGAAACCCTGCAACCGGTGAAAACAA
ATTATTCGGTGAATTTTTAATGAATGCACAAGGCGAAGATGTTGTTGCTGGTATAAGAACTCCTCAACCAATTAGTCAAT
TACAAGACGTAATGCCAGAAGTATATGAACAATTCCATTCAATCACAAAAAGATTAGAAGAGCATTACAGAGATATGCAA
GATTTAGAATTCACAATTGAAAATTCTAAATTATATTTACTTCAAACTAGAAACGGAAAAAGAACTGCTATCAGCGCACT
AAATGTCGCAGTAGATCAAGTAAATGAAGGATTGATAACTAAAGAAGAAGCTATTTTAAGAATTGAACCAAATAGCTTAA
ATCAACTTCTACACCCTACATTTGATCAAAAAGAATTAGAAAATGCTGAAGTAATAACAAAAGGTTTGGCAGCTTCACCT
GGAGCCGCTAGTGGTCACGTTTATTTTAGTGCTGAAGATTGTAAAACAGCTGCTTCTAAGGGAATTAAATCAATATTAGT
TCGTCAAGAAACATCTCCTGAAGATATCGAAGGTATGATTAAAGCCGAAGGTATATTAACTGCAAGAGGAGGTATGACTT
CTCACGCTGCAGTAGTTGCACGAGGAATGGGTGCATGTTGTGTAGCAGGTGCGAGTGAAATAAGGGTAGACGAAGAGAAC
AAAGTAATCAGAGGTAAAAACATAGAAATCAAAGAAGGAGATTTGATTTCATTAGATGGTAGCACTGGATATGTTTATAA
AGGAGAAATTTCTAAAGTAAATCCTAAACTTTCTGGTAACTTTGCCCAATTTATGCAATGGGTTGATGAAATTAAAAGAT
TAGGTGTAAAAACTAATGCAGACACTCCAAAAGATGCACATCAAGCAAAAGAATTTGGTGCTGAAGGCATTGGACTAACA
AGAACTGAGCACATGTTCTTCAATGAAGAAAGAATTCCAATTGTTAGACAAATGATTCTATCAAACACTACTGAAAGAAG
ACAAAAAGCTCTAGATAAATTAAGACCTTTCCAAGAAGATGACTTTTATAATATTTACAAGGAAATGGGAGATTTATCAG
TTACTATAAGACTTTTAGATCCACCTCTTCATGAATTTTTGCCAAAAGAAAAGCACGATATTGAAGAATTAGCAAAAGAA
ATGGATTTAACATATGATGAAGTAAATGATATTATTTTAGAGCTTCATGAAGTAAACCCAATGCTTGGCCACAGAGGATG
TAGATTAGCTGTAACATATCCTGAAATATACAGAATGCAAGCAAGAGCAATCATAAGTGCTGCTCTAAAAGTAAAAGAAG
AAACTTCACATGATATAGTTCCTCAAATCATGATTCCACTTGTAGGAGAAAAGAAAGAATTAAAATATGTTAAAGACCAA
ATAATTGAAGAAATCAATCAAGTATTTGAAGAAACTAATCAAAAATTAGATTACGAAATCGGTACTATGATTGAAATTCC
AAGAGCATGCTTAATGGCTGATGAGATTGCTGAAGAAGCTGACTTCTTCAGTTTTGGTACAAATGACTTAACTCAAATGT
CATTTGGATTCTCTAGGGATGATGCAGGTAAGTTCTTATCAGAATATAAAGAAAAGAAGATTTATTCAAGAAGTCCATTT
GAAAGATTGGATCAAGAAGGTGTTGGTCAGTTAATAAAAATGGCAATTGAAAAAGGTAAGTCTTCAAATCCTAATTTACA
CGTAGGTATTTGTGGTGAACACGGTGGAGATCCTGAAAGCGTGGAATTCTGTCACAGAATTGGATTAGACTATGTATCTT
GTTCCCCATACAGAGTACCAATCGCTAGATTAGCAGCAGCTCAAGCAGCAGCAAGAGAAAAATTAAAATAA

Upstream 100 bases:

>100_bases
GATGTCCTATTATAAATGTTTCTGATAGAGCAATAGAAGAAACAGCAGAAGTTATTATCAGACATCTAAAAAAAATGAAT
GATTAAAAAGGAGTTTTTAA

Downstream 100 bases:

>100_bases
TTATTTGAACTTGATAGAGTAAATTCGTTTACTCTATCTTTTTTTGCTTTTAAAAGAAATTATTATTAAGTAGTTTTATG
GTATAATGAATAAAGTTAAG

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 876; Mature: 875

Protein sequence:

>876_residues
MTEKYVYSFREGNKDMRSLLGGKGANLAEMTNMDIPVPTGFTITTEACNKFYQEDEKLWDGLFEEIKSHIKETEKELNKK
FSDEENPLLFSVRSGAVISMPGMMDTILNLGLNDKSVIGLAKSTNNERFAYDSYRRFIQMFSDVAREIPKVLFENELDKI
KQEKNVTDDTDLDSEDLKKIVEKFKQIYKEEMDEDFPQDPIDQLKYAIEAVFRSWNNPRAKIYRQLNGIDDHLGTAVNIQ
SMVFGNMGNTSGTGVAFSRNPATGENKLFGEFLMNAQGEDVVAGIRTPQPISQLQDVMPEVYEQFHSITKRLEEHYRDMQ
DLEFTIENSKLYLLQTRNGKRTAISALNVAVDQVNEGLITKEEAILRIEPNSLNQLLHPTFDQKELENAEVITKGLAASP
GAASGHVYFSAEDCKTAASKGIKSILVRQETSPEDIEGMIKAEGILTARGGMTSHAAVVARGMGACCVAGASEIRVDEEN
KVIRGKNIEIKEGDLISLDGSTGYVYKGEISKVNPKLSGNFAQFMQWVDEIKRLGVKTNADTPKDAHQAKEFGAEGIGLT
RTEHMFFNEERIPIVRQMILSNTTERRQKALDKLRPFQEDDFYNIYKEMGDLSVTIRLLDPPLHEFLPKEKHDIEELAKE
MDLTYDEVNDIILELHEVNPMLGHRGCRLAVTYPEIYRMQARAIISAALKVKEETSHDIVPQIMIPLVGEKKELKYVKDQ
IIEEINQVFEETNQKLDYEIGTMIEIPRACLMADEIAEEADFFSFGTNDLTQMSFGFSRDDAGKFLSEYKEKKIYSRSPF
ERLDQEGVGQLIKMAIEKGKSSNPNLHVGICGEHGGDPESVEFCHRIGLDYVSCSPYRVPIARLAAAQAAAREKLK

Sequences:

>Translated_876_residues
MTEKYVYSFREGNKDMRSLLGGKGANLAEMTNMDIPVPTGFTITTEACNKFYQEDEKLWDGLFEEIKSHIKETEKELNKK
FSDEENPLLFSVRSGAVISMPGMMDTILNLGLNDKSVIGLAKSTNNERFAYDSYRRFIQMFSDVAREIPKVLFENELDKI
KQEKNVTDDTDLDSEDLKKIVEKFKQIYKEEMDEDFPQDPIDQLKYAIEAVFRSWNNPRAKIYRQLNGIDDHLGTAVNIQ
SMVFGNMGNTSGTGVAFSRNPATGENKLFGEFLMNAQGEDVVAGIRTPQPISQLQDVMPEVYEQFHSITKRLEEHYRDMQ
DLEFTIENSKLYLLQTRNGKRTAISALNVAVDQVNEGLITKEEAILRIEPNSLNQLLHPTFDQKELENAEVITKGLAASP
GAASGHVYFSAEDCKTAASKGIKSILVRQETSPEDIEGMIKAEGILTARGGMTSHAAVVARGMGACCVAGASEIRVDEEN
KVIRGKNIEIKEGDLISLDGSTGYVYKGEISKVNPKLSGNFAQFMQWVDEIKRLGVKTNADTPKDAHQAKEFGAEGIGLT
RTEHMFFNEERIPIVRQMILSNTTERRQKALDKLRPFQEDDFYNIYKEMGDLSVTIRLLDPPLHEFLPKEKHDIEELAKE
MDLTYDEVNDIILELHEVNPMLGHRGCRLAVTYPEIYRMQARAIISAALKVKEETSHDIVPQIMIPLVGEKKELKYVKDQ
IIEEINQVFEETNQKLDYEIGTMIEIPRACLMADEIAEEADFFSFGTNDLTQMSFGFSRDDAGKFLSEYKEKKIYSRSPF
ERLDQEGVGQLIKMAIEKGKSSNPNLHVGICGEHGGDPESVEFCHRIGLDYVSCSPYRVPIARLAAAQAAAREKLK
>Mature_875_residues
TEKYVYSFREGNKDMRSLLGGKGANLAEMTNMDIPVPTGFTITTEACNKFYQEDEKLWDGLFEEIKSHIKETEKELNKKF
SDEENPLLFSVRSGAVISMPGMMDTILNLGLNDKSVIGLAKSTNNERFAYDSYRRFIQMFSDVAREIPKVLFENELDKIK
QEKNVTDDTDLDSEDLKKIVEKFKQIYKEEMDEDFPQDPIDQLKYAIEAVFRSWNNPRAKIYRQLNGIDDHLGTAVNIQS
MVFGNMGNTSGTGVAFSRNPATGENKLFGEFLMNAQGEDVVAGIRTPQPISQLQDVMPEVYEQFHSITKRLEEHYRDMQD
LEFTIENSKLYLLQTRNGKRTAISALNVAVDQVNEGLITKEEAILRIEPNSLNQLLHPTFDQKELENAEVITKGLAASPG
AASGHVYFSAEDCKTAASKGIKSILVRQETSPEDIEGMIKAEGILTARGGMTSHAAVVARGMGACCVAGASEIRVDEENK
VIRGKNIEIKEGDLISLDGSTGYVYKGEISKVNPKLSGNFAQFMQWVDEIKRLGVKTNADTPKDAHQAKEFGAEGIGLTR
TEHMFFNEERIPIVRQMILSNTTERRQKALDKLRPFQEDDFYNIYKEMGDLSVTIRLLDPPLHEFLPKEKHDIEELAKEM
DLTYDEVNDIILELHEVNPMLGHRGCRLAVTYPEIYRMQARAIISAALKVKEETSHDIVPQIMIPLVGEKKELKYVKDQI
IEEINQVFEETNQKLDYEIGTMIEIPRACLMADEIAEEADFFSFGTNDLTQMSFGFSRDDAGKFLSEYKEKKIYSRSPFE
RLDQEGVGQLIKMAIEKGKSSNPNLHVGICGEHGGDPESVEFCHRIGLDYVSCSPYRVPIARLAAAQAAAREKLK

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate. In E.histolytica and C.symbiosus, PPDK functions in the direction of ATP synthesis [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=453, Percent_Identity=26.4900662251656, Blast_Score=118, Evalue=2e-27,
Organism=Escherichia coli, GI1787994, Length=724, Percent_Identity=24.8618784530387, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI48994992, Length=443, Percent_Identity=25.5079006772009, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788726, Length=449, Percent_Identity=23.162583518931, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1789193, Length=435, Percent_Identity=24.1379310344828, Blast_Score=85, Evalue=2e-17,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 98873; Mature: 98742

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKYVYSFREGNKDMRSLLGGKGANLAEMTNMDIPVPTGFTITTEACNKFYQEDEKLWD
CCCCCEEHHHHCCHHHHHHHCCCCCCHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHH
GLFEEIKSHIKETEKELNKKFSDEENPLLFSVRSGAVISMPGMMDTILNLGLNDKSVIGL
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEECCHHHHHHHHCCCCCCCEEEE
AKSTNNERFAYDSYRRFIQMFSDVAREIPKVLFENELDKIKQEKNVTDDTDLDSEDLKKI
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
VEKFKQIYKEEMDEDFPQDPIDQLKYAIEAVFRSWNNPRAKIYRQLNGIDDHLGTAVNIQ
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHCCCEEHH
SMVFGNMGNTSGTGVAFSRNPATGENKLFGEFLMNAQGEDVVAGIRTPQPISQLQDVMPE
HHHCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHH
VYEQFHSITKRLEEHYRDMQDLEFTIENSKLYLLQTRNGKRTAISALNVAVDQVNEGLIT
HHHHHHHHHHHHHHHHCCHHHCEEEEECCEEEEEEECCCCHHHHHHHHHHHHHHCCCCEE
KEEAILRIEPNSLNQLLHPTFDQKELENAEVITKGLAASPGAASGHVYFSAEDCKTAASK
CCCEEEEECCHHHHHHHCCCCCHHHHCCHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHH
GIKSILVRQETSPEDIEGMIKAEGILTARGGMTSHAAVVARGMGACCVAGASEIRVDEEN
HHHHHHHCCCCCHHHHHHHHHHCCEEEECCCCCHHHHHHHHCCCHHHHCCCHHEEECCCC
KVIRGKNIEIKEGDLISLDGSTGYVYKGEISKVNPKLSGNFAQFMQWVDEIKRLGVKTNA
CEECCCCEEECCCCEEEECCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
DTPKDAHQAKEFGAEGIGLTRTEHMFFNEERIPIVRQMILSNTTERRQKALDKLRPFQED
CCCHHHHHHHHHCCCCCCCCCHHHHEECCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCC
DFYNIYKEMGDLSVTIRLLDPPLHEFLPKEKHDIEELAKEMDLTYDEVNDIILELHEVNP
HHHHHHHHCCCCEEEEEEECCCHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCH
MLGHRGCRLAVTYPEIYRMQARAIISAALKVKEETSHDIVPQIMIPLVGEKKELKYVKDQ
HHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHH
IIEEINQVFEETNQKLDYEIGTMIEIPRACLMADEIAEEADFFSFGTNDLTQMSFGFSRD
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCC
DAGKFLSEYKEKKIYSRSPFERLDQEGVGQLIKMAIEKGKSSNPNLHVGICGEHGGDPES
HHHHHHHHHHHHHHCCCCCHHHHCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHH
VEFCHRIGLDYVSCSPYRVPIARLAAAQAAAREKLK
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TEKYVYSFREGNKDMRSLLGGKGANLAEMTNMDIPVPTGFTITTEACNKFYQEDEKLWD
CCCCEEHHHHCCHHHHHHHCCCCCCHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHH
GLFEEIKSHIKETEKELNKKFSDEENPLLFSVRSGAVISMPGMMDTILNLGLNDKSVIGL
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEECCHHHHHHHHCCCCCCCEEEE
AKSTNNERFAYDSYRRFIQMFSDVAREIPKVLFENELDKIKQEKNVTDDTDLDSEDLKKI
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
VEKFKQIYKEEMDEDFPQDPIDQLKYAIEAVFRSWNNPRAKIYRQLNGIDDHLGTAVNIQ
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHCCCEEHH
SMVFGNMGNTSGTGVAFSRNPATGENKLFGEFLMNAQGEDVVAGIRTPQPISQLQDVMPE
HHHCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHH
VYEQFHSITKRLEEHYRDMQDLEFTIENSKLYLLQTRNGKRTAISALNVAVDQVNEGLIT
HHHHHHHHHHHHHHHHCCHHHCEEEEECCEEEEEEECCCCHHHHHHHHHHHHHHCCCCEE
KEEAILRIEPNSLNQLLHPTFDQKELENAEVITKGLAASPGAASGHVYFSAEDCKTAASK
CCCEEEEECCHHHHHHHCCCCCHHHHCCHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHH
GIKSILVRQETSPEDIEGMIKAEGILTARGGMTSHAAVVARGMGACCVAGASEIRVDEEN
HHHHHHHCCCCCHHHHHHHHHHCCEEEECCCCCHHHHHHHHCCCHHHHCCCHHEEECCCC
KVIRGKNIEIKEGDLISLDGSTGYVYKGEISKVNPKLSGNFAQFMQWVDEIKRLGVKTNA
CEECCCCEEECCCCEEEECCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
DTPKDAHQAKEFGAEGIGLTRTEHMFFNEERIPIVRQMILSNTTERRQKALDKLRPFQED
CCCHHHHHHHHHCCCCCCCCCHHHHEECCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCC
DFYNIYKEMGDLSVTIRLLDPPLHEFLPKEKHDIEELAKEMDLTYDEVNDIILELHEVNP
HHHHHHHHCCCCEEEEEEECCCHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCH
MLGHRGCRLAVTYPEIYRMQARAIISAALKVKEETSHDIVPQIMIPLVGEKKELKYVKDQ
HHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHH
IIEEINQVFEETNQKLDYEIGTMIEIPRACLMADEIAEEADFFSFGTNDLTQMSFGFSRD
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCC
DAGKFLSEYKEKKIYSRSPFERLDQEGVGQLIKMAIEKGKSSNPNLHVGICGEHGGDPES
HHHHHHHHHHHHHHCCCCCHHHHCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHH
VEFCHRIGLDYVSCSPYRVPIARLAAAQAAAREKLK
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2176881; 6257292; 11695893; 11468288; 8610096; 9753432 [H]