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The map label for this gene is rsmB [H]

Identifier: 169824361

GI number: 169824361

Start: 727799

End: 729070

Strand: Direct

Name: rsmB [H]

Synonym: FMG_0664

Alternate gene names: 169824361

Gene position: 727799-729070 (Clockwise)

Preceding gene: 169824360

Following gene: 169824362

Centisome position: 40.49

GC content: 26.1

Gene sequence:

>1272_bases
ATGAATATTAGAAATGAAATAATTAATATTACAAATTCCATAAATAATAAATCAGGATATTCTTCTGAAATATTAAAAAC
TTATGATTATGGTGATTTGGATAAAAGAGATATCGCATATATTAAGAAATGTGTTTTTACTGAAATAGAAAATAGATTTT
TCGTAGATTATGTAATATCTAAGTGTAGCGATTTGGAAATAAATAGACTTGATAAGATCGTTCTGGAAATATTAAGAATG
GCAACTACAGAAATACTATTTATGAAAACAGCTGATTATGCAGTGGTTGACGAGGCTGTAGAATTGGCTGGCAGCATAAC
AAGATACAAAGATTATGTTAATGCTGTTTTAAGAGAAGTTTGTAGAATTAATGTGGATTCTGAAGATTTAGAAGATGATA
TAAAATTTTCTATAAATAAGGATTTGCTTAAAATGTTAAATAAGTCTTATTCGAAAAAAACTGTTAGAAAGCTTCTTAAA
TCATTCTTTTATAAATCTGATTTTGTAATCAAACAAAATAAATTAAAAGAAAACAATTTAAAAATAAACCTAGAAAATCA
AGGATTTGCATTAAATGATCATCCGTTTTTAAAAGACAGTTTCATTGTAGAAAATCCAGAATCTATAATAGATACGAAAG
AATTTAAAGAAGGTAAATTTCTTATACAAGATGGTTCATGTAGTTTGTGTGCAGAAATATTAGAACCGTTGGAAAACTCT
ATAATACTTGACATTTGTGCTGCTCCTGGGTCAAAATCATGTAATATTCAAGAAATAACAAACAATCAGTCGATTATTTA
TTGCAATGATATTAATCAGAATAAAATTGCTAGGATTAAGGAGAATGTAGAAAAATGTGCAGTGAAAAATGTTCAATATA
TGAACTTTGATGCGACTACTTTACAAAAAGATTTGATAAATAAATTTGACAGAATTTTAGTGGATGCACCATGTTCTGCT
ACTGGCGTTATAAATAAAAATCCTGAAATAAAGTTATTCAGAACAAAAAAAGATGTTGAAAATCTAGTAAATACACAACG
ACAAATACTTGAAAATTGTGAGAAATATCTAAAAAAAGGTGGCATTTTAGTTTATTCTACTTGTTCACTTTTAAAAGAAG
AAAACGAAGAAAATGTTGAATTTATAGAAAATAGATTACATTTAAAAAGAAAAGACATTGAATTTTATGGAGATAAAAAT
GAATTTATAAAACTAATGCCTTTTCAAAAAGGAACGCAAGGCTTCTTCATTTCAAAGTTTGTAAAGGACTAA

Upstream 100 bases:

>100_bases
TGAACCTAAGAATAATTATTCTAGGTTCAATTTTTTTATTTTTGTAGTTTTAGTGTAAAATATATTATGTAATGAAAATA
GTTAAAAGAAATGAGAGATT

Downstream 100 bases:

>100_bases
TAGATTACAAAACTTGGTATTTTTGATATAATTTAATTTGGTGAATAATATGCAAAATAAAATCATCTTAGAAAATATGA
CTGTTGATGAACTGAAGGAA

Product: putative rRNA methylase

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MNIRNEIINITNSINNKSGYSSEILKTYDYGDLDKRDIAYIKKCVFTEIENRFFVDYVISKCSDLEINRLDKIVLEILRM
ATTEILFMKTADYAVVDEAVELAGSITRYKDYVNAVLREVCRINVDSEDLEDDIKFSINKDLLKMLNKSYSKKTVRKLLK
SFFYKSDFVIKQNKLKENNLKINLENQGFALNDHPFLKDSFIVENPESIIDTKEFKEGKFLIQDGSCSLCAEILEPLENS
IILDICAAPGSKSCNIQEITNNQSIIYCNDINQNKIARIKENVEKCAVKNVQYMNFDATTLQKDLINKFDRILVDAPCSA
TGVINKNPEIKLFRTKKDVENLVNTQRQILENCEKYLKKGGILVYSTCSLLKEENEENVEFIENRLHLKRKDIEFYGDKN
EFIKLMPFQKGTQGFFISKFVKD

Sequences:

>Translated_423_residues
MNIRNEIINITNSINNKSGYSSEILKTYDYGDLDKRDIAYIKKCVFTEIENRFFVDYVISKCSDLEINRLDKIVLEILRM
ATTEILFMKTADYAVVDEAVELAGSITRYKDYVNAVLREVCRINVDSEDLEDDIKFSINKDLLKMLNKSYSKKTVRKLLK
SFFYKSDFVIKQNKLKENNLKINLENQGFALNDHPFLKDSFIVENPESIIDTKEFKEGKFLIQDGSCSLCAEILEPLENS
IILDICAAPGSKSCNIQEITNNQSIIYCNDINQNKIARIKENVEKCAVKNVQYMNFDATTLQKDLINKFDRILVDAPCSA
TGVINKNPEIKLFRTKKDVENLVNTQRQILENCEKYLKKGGILVYSTCSLLKEENEENVEFIENRLHLKRKDIEFYGDKN
EFIKLMPFQKGTQGFFISKFVKD
>Mature_423_residues
MNIRNEIINITNSINNKSGYSSEILKTYDYGDLDKRDIAYIKKCVFTEIENRFFVDYVISKCSDLEINRLDKIVLEILRM
ATTEILFMKTADYAVVDEAVELAGSITRYKDYVNAVLREVCRINVDSEDLEDDIKFSINKDLLKMLNKSYSKKTVRKLLK
SFFYKSDFVIKQNKLKENNLKINLENQGFALNDHPFLKDSFIVENPESIIDTKEFKEGKFLIQDGSCSLCAEILEPLENS
IILDICAAPGSKSCNIQEITNNQSIIYCNDINQNKIARIKENVEKCAVKNVQYMNFDATTLQKDLINKFDRILVDAPCSA
TGVINKNPEIKLFRTKKDVENLVNTQRQILENCEKYLKKGGILVYSTCSLLKEENEENVEFIENRLHLKRKDIEFYGDKN
EFIKLMPFQKGTQGFFISKFVKD

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=234, Percent_Identity=31.1965811965812, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI76150623, Length=234, Percent_Identity=31.1965811965812, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI32698918, Length=230, Percent_Identity=28.2608695652174, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI270288818, Length=300, Percent_Identity=24, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI8922322, Length=280, Percent_Identity=22.8571428571429, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI270288816, Length=280, Percent_Identity=22.8571428571429, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI23199998, Length=280, Percent_Identity=22.8571428571429, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI301336155, Length=183, Percent_Identity=29.5081967213115, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI39995082, Length=183, Percent_Identity=29.5081967213115, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI11545785, Length=172, Percent_Identity=27.906976744186, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI2367212, Length=436, Percent_Identity=27.5229357798165, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI87081985, Length=269, Percent_Identity=30.4832713754647, Blast_Score=126, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17536757, Length=270, Percent_Identity=30.7407407407407, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71998419, Length=224, Percent_Identity=29.4642857142857, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI115534021, Length=224, Percent_Identity=29.4642857142857, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI25143471, Length=203, Percent_Identity=29.5566502463054, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17509941, Length=167, Percent_Identity=29.3413173652695, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324268, Length=235, Percent_Identity=34.0425531914894, Blast_Score=132, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6319447, Length=212, Percent_Identity=28.7735849056604, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI22024126, Length=230, Percent_Identity=32.6086956521739, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI21356579, Length=225, Percent_Identity=27.5555555555556, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24668781, Length=242, Percent_Identity=27.2727272727273, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356513, Length=242, Percent_Identity=27.2727272727273, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21355201, Length=158, Percent_Identity=32.9113924050633, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI19920494, Length=171, Percent_Identity=30.4093567251462, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 48960; Mature: 48960

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIRNEIINITNSINNKSGYSSEILKTYDYGDLDKRDIAYIKKCVFTEIENRFFVDYVIS
CCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
KCSDLEINRLDKIVLEILRMATTEILFMKTADYAVVDEAVELAGSITRYKDYVNAVLREV
CCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CRINVDSEDLEDDIKFSINKDLLKMLNKSYSKKTVRKLLKSFFYKSDFVIKQNKLKENNL
HHCCCCHHHCCCHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCE
KINLENQGFALNDHPFLKDSFIVENPESIIDTKEFKEGKFLIQDGSCSLCAEILEPLENS
EEEECCCCEEECCCCCCCCCEEECCCHHHHCCHHCCCCCEEEECCCHHHHHHHHHHHCCC
IILDICAAPGSKSCNIQEITNNQSIIYCNDINQNKIARIKENVEKCAVKNVQYMNFDATT
EEEEEECCCCCCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEECCCHHH
LQKDLINKFDRILVDAPCSATGVINKNPEIKLFRTKKDVENLVNTQRQILENCEKYLKKG
HHHHHHHHHHHHEEECCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHC
GILVYSTCSLLKEENEENVEFIENRLHLKRKDIEFYGDKNEFIKLMPFQKGTQGFFISKF
CEEEEHHHHHHHHCCCCHHHHHHHHHHHHHCCHHEECCCCCEEEEECCCCCCCCHHHHHH
VKD
HCC
>Mature Secondary Structure
MNIRNEIINITNSINNKSGYSSEILKTYDYGDLDKRDIAYIKKCVFTEIENRFFVDYVIS
CCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
KCSDLEINRLDKIVLEILRMATTEILFMKTADYAVVDEAVELAGSITRYKDYVNAVLREV
CCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CRINVDSEDLEDDIKFSINKDLLKMLNKSYSKKTVRKLLKSFFYKSDFVIKQNKLKENNL
HHCCCCHHHCCCHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCE
KINLENQGFALNDHPFLKDSFIVENPESIIDTKEFKEGKFLIQDGSCSLCAEILEPLENS
EEEECCCCEEECCCCCCCCCEEECCCHHHHCCHHCCCCCEEEECCCHHHHHHHHHHHCCC
IILDICAAPGSKSCNIQEITNNQSIIYCNDINQNKIARIKENVEKCAVKNVQYMNFDATT
EEEEEECCCCCCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEECCCHHH
LQKDLINKFDRILVDAPCSATGVINKNPEIKLFRTKKDVENLVNTQRQILENCEKYLKKG
HHHHHHHHHHHHEEECCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHC
GILVYSTCSLLKEENEENVEFIENRLHLKRKDIEFYGDKNEFIKLMPFQKGTQGFFISKF
CEEEEHHHHHHHHCCCCHHHHHHHHHHHHHCCHHEECCCCCEEEEECCCCCCCCHHHHHH
VKD
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA