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The map label for this gene is rlmN

Identifier: 169824362

GI number: 169824362

Start: 729120

End: 730169

Strand: Direct

Name: rlmN

Synonym: FMG_0665

Alternate gene names: 169824362

Gene position: 729120-730169 (Clockwise)

Preceding gene: 169824361

Following gene: 169824363

Centisome position: 40.56

GC content: 28.57

Gene sequence:

>1050_bases
ATGCAAAATAAAATCATCTTAGAAAATATGACTGTTGATGAACTGAAGGAATTTTTTGTTAACAATGGAGAAAAACCATT
CAGAGCGTTGCAGTATTTTCAAGCTATTCATAAAAATAGAATATTCAATCCGGACGAAATGACTAATTTCTCTAATAGTT
TAAGGGGAAAATTAAATCAGTACAACGATATAAAAAATTGTTCTATCATAAAAAGGATTGATTCCAAACTAGACAACACA
AAAAAATATCTTATTGAGATGAGTGATGGTAATATCGTAGAAACTGTATTTATGCAGTATAAGACTCACACTTCAATATG
CTTGTCAACACAAATTGGTTGCAAGATGGGTTGCAAATTTTGTGCGTCAACAAAGAAGAGTTTTGTAAGAAATTTACAAC
CTTATGAGATGTGCGCTCAAATTTATTTGGTGGAAAATGATTTGGATATAAGAATTAATAATATAGTATTGATGGGAATA
GGCGAACCACTAGATAATTACGACAATGTTTCTAGATTTATTGATTTGATTACTGACAAAGATGGTCAAGATATGTCAAT
TAGAAATATAACATTATCTACTTGCGGATTGGTTGATAAAATTATTAGACTTGCAAACGATGATATAGGTATAAATATAA
CTATTTCACTTCATAATCCATTCGATAATGAGAGAAATAAACTTATGCCAATAGGAAACAAATACTCAATTGAAGAGATT
CTAGATGCATGTGATTACTATTTCAAGAAAACTAAACGACGTATTGGTTTTGAATACACTGTCATTGAAAATGTTAATGA
TTCAAAAAAATACATGGATAAGTTAGTTAGTTTATTGAAAAACAGAAATTGCTTATTGAACTTGATAACATTAAATCCTA
TTGAAGAATTTAATCAAAAATCTCCAGATAGATATAAAATGACAGAATTTATGGAATATATGAACAAAAATAATGTGAAT
ACAACGATAAGAAGAAAACAAGGCATTGATATAGATGGGGCCTGTGGACAATTAAGAATTAACAATATGACAAAAAGAGG
TGTGAAATGA

Upstream 100 bases:

>100_bases
TTTCAAAAAGGAACGCAAGGCTTCTTCATTTCAAAGTTTGTAAAGGACTAATAGATTACAAAACTTGGTATTTTTGATAT
AATTTAATTTGGTGAATAAT

Downstream 100 bases:

>100_bases
AATATGCTTCAAACACAGACATCGGCTTGGTAAGAAAGCTAAATGAAGATTATCATATGAATTATATGACTGATGATTTT
AGCTTACTTGTTGTATGTGA

Product: putative Fe-S-cluster redox protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MQNKIILENMTVDELKEFFVNNGEKPFRALQYFQAIHKNRIFNPDEMTNFSNSLRGKLNQYNDIKNCSIIKRIDSKLDNT
KKYLIEMSDGNIVETVFMQYKTHTSICLSTQIGCKMGCKFCASTKKSFVRNLQPYEMCAQIYLVENDLDIRINNIVLMGI
GEPLDNYDNVSRFIDLITDKDGQDMSIRNITLSTCGLVDKIIRLANDDIGINITISLHNPFDNERNKLMPIGNKYSIEEI
LDACDYYFKKTKRRIGFEYTVIENVNDSKKYMDKLVSLLKNRNCLLNLITLNPIEEFNQKSPDRYKMTEFMEYMNKNNVN
TTIRRKQGIDIDGACGQLRINNMTKRGVK

Sequences:

>Translated_349_residues
MQNKIILENMTVDELKEFFVNNGEKPFRALQYFQAIHKNRIFNPDEMTNFSNSLRGKLNQYNDIKNCSIIKRIDSKLDNT
KKYLIEMSDGNIVETVFMQYKTHTSICLSTQIGCKMGCKFCASTKKSFVRNLQPYEMCAQIYLVENDLDIRINNIVLMGI
GEPLDNYDNVSRFIDLITDKDGQDMSIRNITLSTCGLVDKIIRLANDDIGINITISLHNPFDNERNKLMPIGNKYSIEEI
LDACDYYFKKTKRRIGFEYTVIENVNDSKKYMDKLVSLLKNRNCLLNLITLNPIEEFNQKSPDRYKMTEFMEYMNKNNVN
TTIRRKQGIDIDGACGQLRINNMTKRGVK
>Mature_349_residues
MQNKIILENMTVDELKEFFVNNGEKPFRALQYFQAIHKNRIFNPDEMTNFSNSLRGKLNQYNDIKNCSIIKRIDSKLDNT
KKYLIEMSDGNIVETVFMQYKTHTSICLSTQIGCKMGCKFCASTKKSFVRNLQPYEMCAQIYLVENDLDIRINNIVLMGI
GEPLDNYDNVSRFIDLITDKDGQDMSIRNITLSTCGLVDKIIRLANDDIGINITISLHNPFDNERNKLMPIGNKYSIEEI
LDACDYYFKKTKRRIGFEYTVIENVNDSKKYMDKLVSLLKNRNCLLNLITLNPIEEFNQKSPDRYKMTEFMEYMNKNNVN
TTIRRKQGIDIDGACGQLRINNMTKRGVK

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=359, Percent_Identity=34.2618384401114, Blast_Score=212, Evalue=3e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_FINM2 (B0S143)

Other databases:

- EMBL:   AP008971
- RefSeq:   YP_001691973.1
- ProteinModelPortal:   B0S143
- GeneID:   6019305
- GenomeReviews:   AP008971_GR
- KEGG:   fma:FMG_0665
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- BioCyc:   FMAG334413:FMG_0665-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 40597; Mature: 40597

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNKIILENMTVDELKEFFVNNGEKPFRALQYFQAIHKNRIFNPDEMTNFSNSLRGKLNQ
CCCCEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
YNDIKNCSIIKRIDSKLDNTKKYLIEMSDGNIVETVFMQYKTHTSICLSTQIGCKMGCKF
CCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHH
CASTKKSFVRNLQPYEMCAQIYLVENDLDIRINNIVLMGIGEPLDNYDNVSRFIDLITDK
HHHHHHHHHHCCCHHHHHEEEEEEECCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHCCC
DGQDMSIRNITLSTCGLVDKIIRLANDDIGINITISLHNPFDNERNKLMPIGNKYSIEEI
CCCCCEEEEEEHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHH
LDACDYYFKKTKRRIGFEYTVIENVNDSKKYMDKLVSLLKNRNCLLNLITLNPIEEFNQK
HHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHCCC
SPDRYKMTEFMEYMNKNNVNTTIRRKQGIDIDGACGQLRINNMTKRGVK
CCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEECCCHHCCCC
>Mature Secondary Structure
MQNKIILENMTVDELKEFFVNNGEKPFRALQYFQAIHKNRIFNPDEMTNFSNSLRGKLNQ
CCCCEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
YNDIKNCSIIKRIDSKLDNTKKYLIEMSDGNIVETVFMQYKTHTSICLSTQIGCKMGCKF
CCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHH
CASTKKSFVRNLQPYEMCAQIYLVENDLDIRINNIVLMGIGEPLDNYDNVSRFIDLITDK
HHHHHHHHHHCCCHHHHHEEEEEEECCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHCCC
DGQDMSIRNITLSTCGLVDKIIRLANDDIGINITISLHNPFDNERNKLMPIGNKYSIEEI
CCCCCEEEEEEHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHH
LDACDYYFKKTKRRIGFEYTVIENVNDSKKYMDKLVSLLKNRNCLLNLITLNPIEEFNQK
HHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHCCC
SPDRYKMTEFMEYMNKNNVNTTIRRKQGIDIDGACGQLRINNMTKRGVK
CCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEECCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA