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The map label for this gene is priA [H]

Identifier: 169824357

GI number: 169824357

Start: 723203

End: 725572

Strand: Direct

Name: priA [H]

Synonym: FMG_0660

Alternate gene names: 169824357

Gene position: 723203-725572 (Clockwise)

Preceding gene: 169824356

Following gene: 169824358

Centisome position: 40.23

GC content: 29.62

Gene sequence:

>2370_bases
ATGTATATTGATGTTATTTTTAGAAATAAATCGAAGATGACTAACAGGCTGTACTCTTATCATTCGAATGAAGAAGTTGA
AGTTGGAATGAGAGTAATAGTTCCTTTTGGTAAATCAAATATTAACAAATTGGCATTAGTGGTTAGAGTTAATGAAAACC
CTAATCAAGATATTGATTACAAAGAAATAATATCAGTAGTAGATTTGAATCCTATTGTCACACAAGAATCCATTGACATA
GCTTTTTTTATGATCGATAAATATTTGAGCGATTATAGTTCGGCTTTTCAAACTATATTACCACCTGGTAACTTTGAAGA
ATTAAAAGAGTATTTCGTTGCAAATGATAAACTGAATAATGTTGATGAAGATTTGTACAATATTCTTAAAACAAAGAAAG
AATATTCGGAAATTAAGGAAATTTCAGATATTAGTCACAAAAAACTAATGGAGTATGTTAAATTTGGTTTATTGAAAATG
GATTTAGTGTACGATCAAAAAATTTCTCCTAAATTATTAAAATACGTAAAACTTATAGACAATAATCCAGTTATTTCAAA
AAGAGCAAACGCACAACAAAAAATAGTTGATATGCTTAAAAAGGAAAGCAATATACTGTATAGCGAACTTTTAGCTAAAA
CAAATACGACTTCAAGTGTAGTAAAAGCACTTGAGGAAAAGAAAGTTGTTAAAATTTATTTCAAAGAAGTTGACAGAATT
GTTGTGGATGACACCAAATCTTATCATAAAATAAGCCTAAATTCGCAGCAAGAAAATGCCTACAATTCGATTTTAGAAAA
TCAATTTAATTTGTTACATGGTGTCACTGGTAGTGGAAAAACTGAAGTTTATTTGCATTTGGTTGAGAAAATGTTAAACG
AAGGAAAAGATAGCATAGTTTTGGTTCCAGAAATTTCTTTGACACCTCAGACAATTGAAAGATTTGAGGGAAGATTTCCT
GGGAAAGTTGCTATTATTCATTCTAAACTAAATATTAACGAAAAATTTGAACAATTTAGAAAAATGTATAATGGAGAATA
CAAGATAGTGGTTGGAGCTAGAAGCGCAGTATTTTCCCCTTTTAAAAATTTAGGTTTGATAATAATTGATGAAGAGCATG
AAACAAGTTATGTAAGTGAAAAAAATCCTAAATATAATGCTATCGATATTTCTAGATTTAGAGCAGAATATAATAACGCA
AAGCTTTTATTGGGGTCGGCAACTCCTGGAGTTGAAGAATACAATAATGCTTTAAAAGGTCAGTACAACCTTATAGAAAT
GAACAAAAGAGCAAACAATACAACTCCTGTGGATTGCACGATTGTAGACATGAGAAAAGAATTAGAGACTGGAAACATGA
GAATGTTTAGTAAAGTTCTTCTCAACAAAATTCAAGAAAATTTAAACAACAAAAAACAAACTATTTTGTTTTTAAACAAA
AGAGGCCACACTTCTTTTATTTCGTGTAGAAGATGTGGATATGTATTAAAATGTGATAGCTGCGATGTATCCATGACATA
TCACAAAACAAAAAACAGACTGATTTGTCACATGTGTGGAAGAACTGCATTAAAACCAAAAGTATGTCCTAATTGTGGTT
CGAAATTTATTAAAGAGTTTGGAGCAGGAACGCAAAAACTAGAAGAAGAAACTAGAGAGCTATTTCCAGATGCACGAATT
GTACGAATGGATAGGGACACTACAAACACGAAAAAAGATTACGAAAAAATCTACGATATGATGAATAATCAAGAAATAGA
CATATTAATTGGGACACAAATGTTGTCAAAAGGATTTGATTTTCCAAATGTAACACTAGTCGGTGTAATGGCTGCAGATA
TTAGTTTGAACTTAGGAGATTACAAATCAAACGAAAAAACATTTCAATTGATAACTCAAGTAGCCGGAAGAGCTGGAAGA
GCAGAATATGAAGGAGATGTAATTATACAAACTTATAAACCTGATAATTTTGCTATCACATCAGCAGCGCTTCATGATTA
CAAAATGTTTTATGAAAAGGAGATAAAACTTCGAAAATCATTTAATTATCCTCCTTTTTACAGGATATTAAATATCAATA
TCTCCAGCAGAAATCGAAATGTTGCGATGAAAAAAATCAATGAGGTTTCAAATTATGTTGCTGCATTGATTAGAAGATAT
AAATTATCTACAACATTGATTAATGGTCCTCATCCTTCTCCAATAGAAAGGATAAATAATATGTACAGGTTTGATTTGTA
CTATAAATTCCCTAATGGAGATGATAAAATAATAGAAATAATAAAAAAAGTTTTAATAAACAATGAATACAATATTAACT
TAGAGGGAATAACGCTTAAAATAACATTAGATCCTCAAAGTTATTTTTAA

Upstream 100 bases:

>100_bases
TAAAGTATCTGTAATTAGCAAGGACAATATATTAGATATTGAAAGAGATACAAAATTAAATATCGCAGATAGGATATTGG
ATTTGATAAAGTAGGTAAAC

Downstream 100 bases:

>100_bases
GGAGATAAAATGGCATTAAGACAAATTAGATTAGAAAATGATCCAATCTTGAGAAAAAAATCAAGAGAGGTTGAAAAAAT
AGATGATAGAATCAAACAAA

Product: primosomal protein N

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 789; Mature: 789

Protein sequence:

>789_residues
MYIDVIFRNKSKMTNRLYSYHSNEEVEVGMRVIVPFGKSNINKLALVVRVNENPNQDIDYKEIISVVDLNPIVTQESIDI
AFFMIDKYLSDYSSAFQTILPPGNFEELKEYFVANDKLNNVDEDLYNILKTKKEYSEIKEISDISHKKLMEYVKFGLLKM
DLVYDQKISPKLLKYVKLIDNNPVISKRANAQQKIVDMLKKESNILYSELLAKTNTTSSVVKALEEKKVVKIYFKEVDRI
VVDDTKSYHKISLNSQQENAYNSILENQFNLLHGVTGSGKTEVYLHLVEKMLNEGKDSIVLVPEISLTPQTIERFEGRFP
GKVAIIHSKLNINEKFEQFRKMYNGEYKIVVGARSAVFSPFKNLGLIIIDEEHETSYVSEKNPKYNAIDISRFRAEYNNA
KLLLGSATPGVEEYNNALKGQYNLIEMNKRANNTTPVDCTIVDMRKELETGNMRMFSKVLLNKIQENLNNKKQTILFLNK
RGHTSFISCRRCGYVLKCDSCDVSMTYHKTKNRLICHMCGRTALKPKVCPNCGSKFIKEFGAGTQKLEEETRELFPDARI
VRMDRDTTNTKKDYEKIYDMMNNQEIDILIGTQMLSKGFDFPNVTLVGVMAADISLNLGDYKSNEKTFQLITQVAGRAGR
AEYEGDVIIQTYKPDNFAITSAALHDYKMFYEKEIKLRKSFNYPPFYRILNINISSRNRNVAMKKINEVSNYVAALIRRY
KLSTTLINGPHPSPIERINNMYRFDLYYKFPNGDDKIIEIIKKVLINNEYNINLEGITLKITLDPQSYF

Sequences:

>Translated_789_residues
MYIDVIFRNKSKMTNRLYSYHSNEEVEVGMRVIVPFGKSNINKLALVVRVNENPNQDIDYKEIISVVDLNPIVTQESIDI
AFFMIDKYLSDYSSAFQTILPPGNFEELKEYFVANDKLNNVDEDLYNILKTKKEYSEIKEISDISHKKLMEYVKFGLLKM
DLVYDQKISPKLLKYVKLIDNNPVISKRANAQQKIVDMLKKESNILYSELLAKTNTTSSVVKALEEKKVVKIYFKEVDRI
VVDDTKSYHKISLNSQQENAYNSILENQFNLLHGVTGSGKTEVYLHLVEKMLNEGKDSIVLVPEISLTPQTIERFEGRFP
GKVAIIHSKLNINEKFEQFRKMYNGEYKIVVGARSAVFSPFKNLGLIIIDEEHETSYVSEKNPKYNAIDISRFRAEYNNA
KLLLGSATPGVEEYNNALKGQYNLIEMNKRANNTTPVDCTIVDMRKELETGNMRMFSKVLLNKIQENLNNKKQTILFLNK
RGHTSFISCRRCGYVLKCDSCDVSMTYHKTKNRLICHMCGRTALKPKVCPNCGSKFIKEFGAGTQKLEEETRELFPDARI
VRMDRDTTNTKKDYEKIYDMMNNQEIDILIGTQMLSKGFDFPNVTLVGVMAADISLNLGDYKSNEKTFQLITQVAGRAGR
AEYEGDVIIQTYKPDNFAITSAALHDYKMFYEKEIKLRKSFNYPPFYRILNINISSRNRNVAMKKINEVSNYVAALIRRY
KLSTTLINGPHPSPIERINNMYRFDLYYKFPNGDDKIIEIIKKVLINNEYNINLEGITLKITLDPQSYF
>Mature_789_residues
MYIDVIFRNKSKMTNRLYSYHSNEEVEVGMRVIVPFGKSNINKLALVVRVNENPNQDIDYKEIISVVDLNPIVTQESIDI
AFFMIDKYLSDYSSAFQTILPPGNFEELKEYFVANDKLNNVDEDLYNILKTKKEYSEIKEISDISHKKLMEYVKFGLLKM
DLVYDQKISPKLLKYVKLIDNNPVISKRANAQQKIVDMLKKESNILYSELLAKTNTTSSVVKALEEKKVVKIYFKEVDRI
VVDDTKSYHKISLNSQQENAYNSILENQFNLLHGVTGSGKTEVYLHLVEKMLNEGKDSIVLVPEISLTPQTIERFEGRFP
GKVAIIHSKLNINEKFEQFRKMYNGEYKIVVGARSAVFSPFKNLGLIIIDEEHETSYVSEKNPKYNAIDISRFRAEYNNA
KLLLGSATPGVEEYNNALKGQYNLIEMNKRANNTTPVDCTIVDMRKELETGNMRMFSKVLLNKIQENLNNKKQTILFLNK
RGHTSFISCRRCGYVLKCDSCDVSMTYHKTKNRLICHMCGRTALKPKVCPNCGSKFIKEFGAGTQKLEEETRELFPDARI
VRMDRDTTNTKKDYEKIYDMMNNQEIDILIGTQMLSKGFDFPNVTLVGVMAADISLNLGDYKSNEKTFQLITQVAGRAGR
AEYEGDVIIQTYKPDNFAITSAALHDYKMFYEKEIKLRKSFNYPPFYRILNINISSRNRNVAMKKINEVSNYVAALIRRY
KLSTTLINGPHPSPIERINNMYRFDLYYKFPNGDDKIIEIIKKVLINNEYNINLEGITLKITLDPQSYF

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=579, Percent_Identity=34.8877374784111, Blast_Score=384, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 91090; Mature: 91090

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYIDVIFRNKSKMTNRLYSYHSNEEVEVGMRVIVPFGKSNINKLALVVRVNENPNQDIDY
CEEEEEECCCHHHHHHHHHCCCCCCEEECEEEEEECCCCCCCEEEEEEEECCCCCCCCCH
KEIISVVDLNPIVTQESIDIAFFMIDKYLSDYSSAFQTILPPGNFEELKEYFVANDKLNN
HHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCC
VDEDLYNILKTKKEYSEIKEISDISHKKLMEYVKFGLLKMDLVYDQKISPKLLKYVKLID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHC
NNPVISKRANAQQKIVDMLKKESNILYSELLAKTNTTSSVVKALEEKKVVKIYFKEVDRI
CCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHCEE
VVDDTKSYHKISLNSQQENAYNSILENQFNLLHGVTGSGKTEVYLHLVEKMLNEGKDSIV
EECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEE
LVPEISLTPQTIERFEGRFPGKVAIIHSKLNINEKFEQFRKMYNGEYKIVVGARSAVFSP
EEECCCCCHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECHHHHHHH
FKNLGLIIIDEEHETSYVSEKNPKYNAIDISRFRAEYNNAKLLLGSATPGVEEYNNALKG
HHHCCEEEEECCCCCCCCCCCCCCCCEEEHHHHHHHCCCCEEEEECCCCCHHHHHHHHCC
QYNLIEMNKRANNTTPVDCTIVDMRKELETGNMRMFSKVLLNKIQENLNNKKQTILFLNK
CEEEEEECCCCCCCCCCEEEEEEHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEC
RGHTSFISCRRCGYVLKCDSCDVSMTYHKTKNRLICHMCGRTALKPKVCPNCGSKFIKEF
CCCCCHHHHHHCCEEEEECCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHHH
GAGTQKLEEETRELFPDARIVRMDRDTTNTKKDYEKIYDMMNNQEIDILIGTQMLSKGFD
CCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEEHHHHHCCCC
FPNVTLVGVMAADISLNLGDYKSNEKTFQLITQVAGRAGRAEYEGDVIIQTYKPDNFAIT
CCCEEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEE
SAALHDYKMFYEKEIKLRKSFNYPPFYRILNINISSRNRNVAMKKINEVSNYVAALIRRY
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
KLSTTLINGPHPSPIERINNMYRFDLYYKFPNGDDKIIEIIKKVLINNEYNINLEGITLK
HHHEEEECCCCCCHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEEEEE
ITLDPQSYF
EEECCCCCC
>Mature Secondary Structure
MYIDVIFRNKSKMTNRLYSYHSNEEVEVGMRVIVPFGKSNINKLALVVRVNENPNQDIDY
CEEEEEECCCHHHHHHHHHCCCCCCEEECEEEEEECCCCCCCEEEEEEEECCCCCCCCCH
KEIISVVDLNPIVTQESIDIAFFMIDKYLSDYSSAFQTILPPGNFEELKEYFVANDKLNN
HHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCC
VDEDLYNILKTKKEYSEIKEISDISHKKLMEYVKFGLLKMDLVYDQKISPKLLKYVKLID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHC
NNPVISKRANAQQKIVDMLKKESNILYSELLAKTNTTSSVVKALEEKKVVKIYFKEVDRI
CCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHCEE
VVDDTKSYHKISLNSQQENAYNSILENQFNLLHGVTGSGKTEVYLHLVEKMLNEGKDSIV
EECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEE
LVPEISLTPQTIERFEGRFPGKVAIIHSKLNINEKFEQFRKMYNGEYKIVVGARSAVFSP
EEECCCCCHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECHHHHHHH
FKNLGLIIIDEEHETSYVSEKNPKYNAIDISRFRAEYNNAKLLLGSATPGVEEYNNALKG
HHHCCEEEEECCCCCCCCCCCCCCCCEEEHHHHHHHCCCCEEEEECCCCCHHHHHHHHCC
QYNLIEMNKRANNTTPVDCTIVDMRKELETGNMRMFSKVLLNKIQENLNNKKQTILFLNK
CEEEEEECCCCCCCCCCEEEEEEHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEC
RGHTSFISCRRCGYVLKCDSCDVSMTYHKTKNRLICHMCGRTALKPKVCPNCGSKFIKEF
CCCCCHHHHHHCCEEEEECCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHHH
GAGTQKLEEETRELFPDARIVRMDRDTTNTKKDYEKIYDMMNNQEIDILIGTQMLSKGFD
CCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEEHHHHHCCCC
FPNVTLVGVMAADISLNLGDYKSNEKTFQLITQVAGRAGRAEYEGDVIIQTYKPDNFAIT
CCCEEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEE
SAALHDYKMFYEKEIKLRKSFNYPPFYRILNINISSRNRNVAMKKINEVSNYVAALIRRY
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
KLSTTLINGPHPSPIERINNMYRFDLYYKFPNGDDKIIEIIKKVLINNEYNINLEGITLK
HHHEEEECCCCCCHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEEEEE
ITLDPQSYF
EEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]