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Identifier: 169824358

GI number: 169824358

Start: 725582

End: 726070

Strand: Direct

Name: def

Synonym: FMG_0661

Alternate gene names: 169824358

Gene position: 725582-726070 (Clockwise)

Preceding gene: 169824357

Following gene: 169824359

Centisome position: 40.36

GC content: 31.08

Gene sequence:

>489_bases
ATGGCATTAAGACAAATTAGATTAGAAAATGATCCAATCTTGAGAAAAAAATCAAGAGAGGTTGAAAAAATAGATGATAG
AATCAAACAAATCGTAGAAGATATGTTTGAAACGATGTATGAAAATAAAGGAATAGGATTGGCTTGTGTTCAAGTTGGAA
TGCTTAAGAGAATAGTAGTAATAGACATGCAAGATGAAGACGGGAAAATGGTTTTGATAAATCCTAAAATAATAGAAAAA
TCAGAAGAGAAACAAATTAATATTGAAGGTTGTCTATCTGTTCCTGGGAAAAATGGATACGTGGAAAGACCGAAGACAGT
TGTTGTAGAATACACTGATTTAAATGGAAACACTCAAAGAGTAATGGGTACTGATTACAAAGCACATTGTTTTTGTCATG
AATTAGATCACTTGGATGGCGTATTGTACACTGATAAAGTATTGAATCTTTCTGAAGAAGAAGTTGAGAGATTGAATAAT
GAAAAATAA

Upstream 100 bases:

>100_bases
AATAAAAAAAGTTTTAATAAACAATGAATACAATATTAACTTAGAGGGAATAACGCTTAAAATAACATTAGATCCTCAAA
GTTATTTTTAAGGAGATAAA

Downstream 100 bases:

>100_bases
AATTGTATTTACAGGTTCGCCTGATTTTGCAGTTGAGAGTTTGCAAAGATTATATGATAATCCAAATAACGAAATTCAAT
TAGTAATATCACAAGAAGAT

Product: polypeptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 162; Mature: 161

Protein sequence:

>162_residues
MALRQIRLENDPILRKKSREVEKIDDRIKQIVEDMFETMYENKGIGLACVQVGMLKRIVVIDMQDEDGKMVLINPKIIEK
SEEKQINIEGCLSVPGKNGYVERPKTVVVEYTDLNGNTQRVMGTDYKAHCFCHELDHLDGVLYTDKVLNLSEEEVERLNN
EK

Sequences:

>Translated_162_residues
MALRQIRLENDPILRKKSREVEKIDDRIKQIVEDMFETMYENKGIGLACVQVGMLKRIVVIDMQDEDGKMVLINPKIIEK
SEEKQINIEGCLSVPGKNGYVERPKTVVVEYTDLNGNTQRVMGTDYKAHCFCHELDHLDGVLYTDKVLNLSEEEVERLNN
EK
>Mature_161_residues
ALRQIRLENDPILRKKSREVEKIDDRIKQIVEDMFETMYENKGIGLACVQVGMLKRIVVIDMQDEDGKMVLINPKIIEKS
EEKQINIEGCLSVPGKNGYVERPKTVVVEYTDLNGNTQRVMGTDYKAHCFCHELDHLDGVLYTDKVLNLSEEEVERLNNE
K

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Escherichia coli, GI1789682, Length=156, Percent_Identity=40.3846153846154, Blast_Score=112, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24645728, Length=158, Percent_Identity=31.6455696202532, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24645726, Length=158, Percent_Identity=29.746835443038, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_FINM2 (B0S139)

Other databases:

- EMBL:   AP008971
- RefSeq:   YP_001691969.1
- ProteinModelPortal:   B0S139
- SMR:   B0S139
- GeneID:   6019307
- GenomeReviews:   AP008971_GR
- KEGG:   fma:FMG_0661
- HOGENOM:   HBG665227
- OMA:   AGTERYD
- BioCyc:   FMAG334413:FMG_0661-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 18712; Mature: 18581

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: ACT_SITE 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALRQIRLENDPILRKKSREVEKIDDRIKQIVEDMFETMYENKGIGLACVQVGMLKRIVV
CCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCEEEE
IDMQDEDGKMVLINPKIIEKSEEKQINIEGCLSVPGKNGYVERPKTVVVEYTDLNGNTQR
EEEECCCCCEEEECCHHHCCCCCCEECEEEEEECCCCCCCCCCCCEEEEEEECCCCCCCE
VMGTDYKAHCFCHELDHLDGVLYTDKVLNLSEEEVERLNNEK
EECCCCHHHHHHHHHHHCCCEEEEHHHCCCCHHHHHHHCCCC
>Mature Secondary Structure 
ALRQIRLENDPILRKKSREVEKIDDRIKQIVEDMFETMYENKGIGLACVQVGMLKRIVV
CCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCEEEE
IDMQDEDGKMVLINPKIIEKSEEKQINIEGCLSVPGKNGYVERPKTVVVEYTDLNGNTQR
EEEECCCCCEEEECCHHHCCCCCCEECEEEEEECCCCCCCCCCCCEEEEEEECCCCCCCE
VMGTDYKAHCFCHELDHLDGVLYTDKVLNLSEEEVERLNNEK
EECCCCHHHHHHHHHHHCCCEEEEHHHCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA