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The map label for this gene is nucH [H]

Identifier: 169824341

GI number: 169824341

Start: 707433

End: 708122

Strand: Direct

Name: nucH [H]

Synonym: FMG_0644

Alternate gene names: 169824341

Gene position: 707433-708122 (Clockwise)

Preceding gene: 169824340

Following gene: 169824342

Centisome position: 39.35

GC content: 28.41

Gene sequence:

>690_bases
ATGAAGAAATTTTTACTGGGAATATTAATATTAATATCATTAACTGGCTGTGACAAATTTGGTCAAGTTGAAGCTGAAAA
TAATTTGTACGAAGTAGTTAGAGTTGTAGATGGAGACACTGTTATATTAAACATAGACGGCAATAAAACTAGAGTGAGAC
TCATTGGAATTGATACACCGGAAAGTGTAGCCAAAGATAAAAGTCGAAACGTCAAAGAAGGTAAGGTTGCAAGTGATTAT
ACAAAAAGATTGCTCGAAAACAAAAGAGTGCGATTGGAATACGATGATGAAAAAAATGATGTATATGACAGAAAACTGGG
TTATTTGTTTTTAGATGATGAATTTATCAATGAAAAACTACTCAAAGAAGGTATGGCAAAATTATACACAAAAACAACTA
ATCAAAAATATTATGAAAGACTAAAAAAAGCAGAACAATACGCAAAAGATAATAAGAAGGGATTCTGGAAAGATTTCTAT
GTTAATGATACAAATGTATATAAAAAATATATAGATTCTAAAGGTAGGGGAATGATAAAAGGAAATATAAACAGTAAAGG
ATTAAAAATATACCACTTGCCAAATCAAAAAAGTTATAAAGATGTAAAAATTAACTTCAAAAAAGGCGAAAAATATTTTG
TAACAGAAAAAGAAGCGCAAGAAGAAGGATTTTCAAAATCTTCAAAATAA

Upstream 100 bases:

>100_bases
TGCAAGTGATAAGAATTGGGGAGAAAAAGTAAACAATCACATGATACTAATAGCACAAAAGATCTTATCGAGCTATAAAT
AATTAAAATAGAGAGCAAGC

Downstream 100 bases:

>100_bases
TACTTGCAAAACTAAAATTACGATGCTATAATATTAAAGTCAAGTTGACTTGAAATAAATTTTACAAATGCTTGACAATC
ATAGTTTATTATGGTACACT

Product: thermonuclease

Products: NA

Alternate protein names: TNase; Micrococcal nuclease; Staphylococcal nuclease [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MKKFLLGILILISLTGCDKFGQVEAENNLYEVVRVVDGDTVILNIDGNKTRVRLIGIDTPESVAKDKSRNVKEGKVASDY
TKRLLENKRVRLEYDDEKNDVYDRKLGYLFLDDEFINEKLLKEGMAKLYTKTTNQKYYERLKKAEQYAKDNKKGFWKDFY
VNDTNVYKKYIDSKGRGMIKGNINSKGLKIYHLPNQKSYKDVKINFKKGEKYFVTEKEAQEEGFSKSSK

Sequences:

>Translated_229_residues
MKKFLLGILILISLTGCDKFGQVEAENNLYEVVRVVDGDTVILNIDGNKTRVRLIGIDTPESVAKDKSRNVKEGKVASDY
TKRLLENKRVRLEYDDEKNDVYDRKLGYLFLDDEFINEKLLKEGMAKLYTKTTNQKYYERLKKAEQYAKDNKKGFWKDFY
VNDTNVYKKYIDSKGRGMIKGNINSKGLKIYHLPNQKSYKDVKINFKKGEKYFVTEKEAQEEGFSKSSK
>Mature_229_residues
MKKFLLGILILISLTGCDKFGQVEAENNLYEVVRVVDGDTVILNIDGNKTRVRLIGIDTPESVAKDKSRNVKEGKVASDY
TKRLLENKRVRLEYDDEKNDVYDRKLGYLFLDDEFINEKLLKEGMAKLYTKTTNQKYYERLKKAEQYAKDNKKGFWKDFY
VNDTNVYKKYIDSKGRGMIKGNINSKGLKIYHLPNQKSYKDVKINFKKGEKYFVTEKEAQEEGFSKSSK

Specific function: Enzyme that catalyzes the hydrolysis of both DNA and RNA at the 5'-position of the phosphodiester bond [H]

COG id: COG1525

COG function: function code L; Micrococcal nuclease (thermonuclease) homologs

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thermonuclease family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016071
- InterPro:   IPR006021
- InterPro:   IPR002071 [H]

Pfam domain/function: PF00565 SNase [H]

EC number: =3.1.31.1 [H]

Molecular weight: Translated: 26712; Mature: 26712

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01123 TNASE_1 ; PS50830 TNASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFLLGILILISLTGCDKFGQVEAENNLYEVVRVVDGDTVILNIDGNKTRVRLIGIDTP
CHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHEEECCCEEEEEECCCCEEEEEEECCCC
ESVAKDKSRNVKEGKVASDYTKRLLENKRVRLEYDDEKNDVYDRKLGYLFLDDEFINEKL
HHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHCCEEEECHHHHHHHH
LKEGMAKLYTKTTNQKYYERLKKAEQYAKDNKKGFWKDFYVNDTNVYKKYIDSKGRGMIK
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHCCCCCEEEE
GNINSKGLKIYHLPNQKSYKDVKINFKKGEKYFVTEKEAQEEGFSKSSK
ECCCCCCEEEEECCCCCCCCEEEEEEECCCEEEEECHHHHHCCCCCCCC
>Mature Secondary Structure
MKKFLLGILILISLTGCDKFGQVEAENNLYEVVRVVDGDTVILNIDGNKTRVRLIGIDTP
CHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHEEECCCEEEEEECCCCEEEEEEECCCC
ESVAKDKSRNVKEGKVASDYTKRLLENKRVRLEYDDEKNDVYDRKLGYLFLDDEFINEKL
HHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHCCEEEECHHHHHHHH
LKEGMAKLYTKTTNQKYYERLKKAEQYAKDNKKGFWKDFYVNDTNVYKKYIDSKGRGMIK
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHCCCCCEEEE
GNINSKGLKIYHLPNQKSYKDVKINFKKGEKYFVTEKEAQEEGFSKSSK
ECCCCCCEEEEECCCCCCCCEEEEEEECCCEEEEECHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8045422 [H]