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The map label for this gene is murD [H]

Identifier: 169824312

GI number: 169824312

Start: 678879

End: 680204

Strand: Direct

Name: murD [H]

Synonym: FMG_0615

Alternate gene names: 169824312

Gene position: 678879-680204 (Clockwise)

Preceding gene: 169824311

Following gene: 169824313

Centisome position: 37.77

GC content: 29.19

Gene sequence:

>1326_bases
ATGAAGAAAATATTAGTATTTGGATTAGCAAGAACCGGAATATCAGCTCTAAAAACATTAAAATTAAAAGGATATAATTT
AGGAGCAATCGATGATAATATTGACGAAGAAAAAACAAAAATATTAAATGAATTAGACGTTCAAATAGAATCTATAGATA
CTATTGATAAGTACGATATTATTTTAAAAAGTCCTGGCATTAGAATGGATAATCCTGTAATATTAAAGGCTAATGAATTA
AATATCGAAGTTGTTAGCGATTTGGAATTGGCACAAAGAATTTTTGGAGATATTAAAATTGTAGCTATTACTGGTACAAA
TGGCAAAACAACAACAACTTCATTGATGACAAAAATGTTGAATGATTCAGGTAGAAAAGCAATTTCAATAGGTAATATAG
GAGTAGGTATGTTGTGGGAAATCTACAACAGCGATAAGGACACATATTTTGTTATTGAATGCTCGAGCTTCCAACTAGAA
AGCACAAAAAATTTCAAACCACAATATTCATGCATAATCAATATTACTCCAGATCATATTGATTGGCATGGGAGTATGCA
AAATTATGTAAATGCAAAAAAGAATGTACTAAAAAATCAAGACGAAAATTGCTACACTGTACTTAATGCAGATGACGAAT
ACTATGAAGAATGCAAAAAATTTACAAAAGCAAATGTATACGATATTTCTACATCAAAAAATGTTAAAAGAGGCTCATTC
ATAAAAGATAATTCTATATATTTTTCTGATTCAGATTGCGATAAAATCATTGATTTAAAAGATGTTAAATTAATAGGCTA
TCATAATTATCAAAATGTTTTATTCTGCGTTACTTTAGCAAAATTGATAGGAATAAATGATGATAGTATTAGAGAAACTT
TAATGAGCTTTTCTGGAGTAGAACACAGATTGGAATTTGTAAGAGATTTGTCTGGAGTAAAATACTACAACGATTCTAAG
GGAACAAATGTAGATGCAAGTGTTAAGGCAATAGAATCTTTTGATAAAAATGTTATTATACTGGCCGGTGGATATGACAA
AAAAGTTAGCCTGGATAATTTTTTCATAGCTGGTAAAGATAAATTTAAAGCTTTGATTTTAATGGGACAAACTAGAGATT
TGTTCGAACAAAAAGCTAAAGAATATGGATTTAAGCATATCTATTTGGTAGATGATATGAAACAAGCAGTAACTCAAGCT
AACAAAATAGCTGAAAGTGGAGATGTCGTTCTACTTTCTCCAGCTAGTGCAAGTTGGGGAATGTATAATAACTATGAAGA
AAGAGGAAACGAATTTAAAGATTTAGTAAATAATTTGCGAGGATAG

Upstream 100 bases:

>100_bases
GAATTAAAGGGATATAAAGAACAAAAAATAGTATCAACTTTTATGGTTGTATCAGTAATTACAAGTTTAATTACATTATT
AGATATATTGTAGGTGAAAT

Downstream 100 bases:

>100_bases
AGGCATGAAACTTTCAAAAGATGGGAAGGCTTTATTGTATATAACAGTTTTCCTGACAATTTTTGGAATAATTATGGTAC
TAAGTTCAAGCTGGCCAACA

Product: UDP-N-acetylmuramoylalanine-D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MKKILVFGLARTGISALKTLKLKGYNLGAIDDNIDEEKTKILNELDVQIESIDTIDKYDIILKSPGIRMDNPVILKANEL
NIEVVSDLELAQRIFGDIKIVAITGTNGKTTTTSLMTKMLNDSGRKAISIGNIGVGMLWEIYNSDKDTYFVIECSSFQLE
STKNFKPQYSCIINITPDHIDWHGSMQNYVNAKKNVLKNQDENCYTVLNADDEYYEECKKFTKANVYDISTSKNVKRGSF
IKDNSIYFSDSDCDKIIDLKDVKLIGYHNYQNVLFCVTLAKLIGINDDSIRETLMSFSGVEHRLEFVRDLSGVKYYNDSK
GTNVDASVKAIESFDKNVIILAGGYDKKVSLDNFFIAGKDKFKALILMGQTRDLFEQKAKEYGFKHIYLVDDMKQAVTQA
NKIAESGDVVLLSPASASWGMYNNYEERGNEFKDLVNNLRG

Sequences:

>Translated_441_residues
MKKILVFGLARTGISALKTLKLKGYNLGAIDDNIDEEKTKILNELDVQIESIDTIDKYDIILKSPGIRMDNPVILKANEL
NIEVVSDLELAQRIFGDIKIVAITGTNGKTTTTSLMTKMLNDSGRKAISIGNIGVGMLWEIYNSDKDTYFVIECSSFQLE
STKNFKPQYSCIINITPDHIDWHGSMQNYVNAKKNVLKNQDENCYTVLNADDEYYEECKKFTKANVYDISTSKNVKRGSF
IKDNSIYFSDSDCDKIIDLKDVKLIGYHNYQNVLFCVTLAKLIGINDDSIRETLMSFSGVEHRLEFVRDLSGVKYYNDSK
GTNVDASVKAIESFDKNVIILAGGYDKKVSLDNFFIAGKDKFKALILMGQTRDLFEQKAKEYGFKHIYLVDDMKQAVTQA
NKIAESGDVVLLSPASASWGMYNNYEERGNEFKDLVNNLRG
>Mature_441_residues
MKKILVFGLARTGISALKTLKLKGYNLGAIDDNIDEEKTKILNELDVQIESIDTIDKYDIILKSPGIRMDNPVILKANEL
NIEVVSDLELAQRIFGDIKIVAITGTNGKTTTTSLMTKMLNDSGRKAISIGNIGVGMLWEIYNSDKDTYFVIECSSFQLE
STKNFKPQYSCIINITPDHIDWHGSMQNYVNAKKNVLKNQDENCYTVLNADDEYYEECKKFTKANVYDISTSKNVKRGSF
IKDNSIYFSDSDCDKIIDLKDVKLIGYHNYQNVLFCVTLAKLIGINDDSIRETLMSFSGVEHRLEFVRDLSGVKYYNDSK
GTNVDASVKAIESFDKNVIILAGGYDKKVSLDNFFIAGKDKFKALILMGQTRDLFEQKAKEYGFKHIYLVDDMKQAVTQA
NKIAESGDVVLLSPASASWGMYNNYEERGNEFKDLVNNLRG

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=464, Percent_Identity=31.0344827586207, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1786279, Length=289, Percent_Identity=25.9515570934256, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 49754; Mature: 49754

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00018 EF_HAND_1 ; PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILVFGLARTGISALKTLKLKGYNLGAIDDNIDEEKTKILNELDVQIESIDTIDKYDI
CCEEEEEEEHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHHHCCEEEECCCCCCEEE
ILKSPGIRMDNPVILKANELNIEVVSDLELAQRIFGDIKIVAITGTNGKTTTTSLMTKML
EEECCCCCCCCCEEEEECCCCEEEECHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHH
NDSGRKAISIGNIGVGMLWEIYNSDKDTYFVIECSSFQLESTKNFKPQYSCIINITPDHI
CCCCCCEEEECCCCCEEEEEEECCCCCEEEEEEECCEEECCCCCCCCCEEEEEEECCCCC
DWHGSMQNYVNAKKNVLKNQDENCYTVLNADDEYYEECKKFTKANVYDISTSKNVKRGSF
CCCCCHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEECCCCCCCCCCE
IKDNSIYFSDSDCDKIIDLKDVKLIGYHNYQNVLFCVTLAKLIGINDDSIRETLMSFSGV
EECCEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCH
EHRLEFVRDLSGVKYYNDSKGTNVDASVKAIESFDKNVIILAGGYDKKVSLDNFFIAGKD
HHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCCEEECCCEEEECCC
KFKALILMGQTRDLFEQKAKEYGFKHIYLVDDMKQAVTQANKIAESGDVVLLSPASASWG
CEEEEEEECCCHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCCEEEEECCCCCCC
MYNNYEERGNEFKDLVNNLRG
CCCCHHHHCHHHHHHHHHHCC
>Mature Secondary Structure
MKKILVFGLARTGISALKTLKLKGYNLGAIDDNIDEEKTKILNELDVQIESIDTIDKYDI
CCEEEEEEEHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHHHCCEEEECCCCCCEEE
ILKSPGIRMDNPVILKANELNIEVVSDLELAQRIFGDIKIVAITGTNGKTTTTSLMTKML
EEECCCCCCCCCEEEEECCCCEEEECHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHH
NDSGRKAISIGNIGVGMLWEIYNSDKDTYFVIECSSFQLESTKNFKPQYSCIINITPDHI
CCCCCCEEEECCCCCEEEEEEECCCCCEEEEEEECCEEECCCCCCCCCEEEEEEECCCCC
DWHGSMQNYVNAKKNVLKNQDENCYTVLNADDEYYEECKKFTKANVYDISTSKNVKRGSF
CCCCCHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEECCCCCCCCCCE
IKDNSIYFSDSDCDKIIDLKDVKLIGYHNYQNVLFCVTLAKLIGINDDSIRETLMSFSGV
EECCEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCH
EHRLEFVRDLSGVKYYNDSKGTNVDASVKAIESFDKNVIILAGGYDKKVSLDNFFIAGKD
HHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCCEEECCCEEEECCC
KFKALILMGQTRDLFEQKAKEYGFKHIYLVDDMKQAVTQANKIAESGDVVLLSPASASWG
CEEEEEEECCCHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCCEEEEECCCCCCC
MYNNYEERGNEFKDLVNNLRG
CCCCHHHHCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]