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The map label for this gene is gspE [H]

Identifier: 169823947

GI number: 169823947

Start: 294770

End: 295930

Strand: Direct

Name: gspE [H]

Synonym: FMG_0250

Alternate gene names: 169823947

Gene position: 294770-295930 (Clockwise)

Preceding gene: 169823945

Following gene: 169823948

Centisome position: 16.4

GC content: 33.51

Gene sequence:

>1161_bases
ATGGAAATTAACGATAATATTTTATTTGTAAATTCTATAATTGATGATGCAATCAGAAAAAAATCTAGCGATATTCACAT
TGAACCATTTGAAGAAAATGTCAGGATTAGATTTCGAATCAACGGAAGTTTGGTGGAATACAAAACTGTTGACAAGAGCA
TGCATTCAAAGATAATTACTAGAATTAAAATAATGAGTGAGCTTGATATTGCAGAAAAGAGGCTTCCACAAGACGGAAGA
ATTACATATACTTACGAAAATTCGAAGATAGATCTTAGAATTAGCATTATCAACACAATTCACGGCCAAAAATGCGTTAT
TAGAATTTTGAATCCAGAATCTTTTAAAATCGGTATGCAAAATCTTGGAATTTCTGAAAATGAACAAAAAATAATAGAAG
GATTTACGAAATTTGAAAATGGTATGGTGCTTATTTGTGGGCCGACAGGTAGCGGTAAGACCACAACACTTTACACAATG
TTAGAACAAGAAAACACAATCGATAAAAATATTATCACAGTTGAAAATCCAGTTGAGTACAATATTTTTGGGATCAATCA
AATTCAAATCAACGAAAAAATTGGATTGAGATTTGACAATACGTTGAAGTTTGTACTTAGACAAGACCCTTCCGTAATAA
TGGTCGGAGAAATCAGAGATTTGGAAACTTCCAAGCTTGCAATCAGAGCGTCAATCACAGGCCACAAAGTTTACTCCACA
ATTCACACCGACGACTGCTATCAATCGATAATTAGACTGATGGATATGGGAATTGAAGATTACATGATAAGAGCATCACT
CAAAGTTGTCATCGCACAAAGATTGGTCAGAAAATTGTGCCCTCATTGCAAGAAAAAAGTCACACCAACCTCTGAACAAA
TGGAAATTTTTAGATCAAACGGGTTAAATCCAACTGAAATTTACGAGCCAGGAAATTGCGATGAATGCAGTTTGGGTTAC
GATGGGAGAATGGCTGTCATGCAAATATTAAAAATCGATGATGATTTGAGAAGTTTAATAAAAAAAGATATGAATTTGAA
ATCATTGAAAAACTACGGAGAAGAAAATAACATCGACAGTTTATTCAAAAAAGCTTTGATACAAGTAGCACTTGGAAACA
CTTCATTTGACGAAGCGATGAGGTTGTACAATGAAGATTGA

Upstream 100 bases:

>100_bases
TTGATCTTTATATAAAATGATTTTTGGAAACAAATAAAGGAAAGTTTAAATTTGTTTCCAAATTTTATTTGTTTTTTATT
GTATATACAAGGAGACAACA

Downstream 100 bases:

>100_bases
CAGTTTGCTAAAAGAAGACAGTGTATTAAAAAAAGAAATAAACTTCAAGGCTTCCAAGAAAAAGCTGTTTTTGTTTCTGA
GACAATTTTCAATTCTTTTG

Product: putative pili biogenesis ATPase

Products: NA

Alternate protein names: Type II traffic warden ATPase [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MEINDNILFVNSIIDDAIRKKSSDIHIEPFEENVRIRFRINGSLVEYKTVDKSMHSKIITRIKIMSELDIAEKRLPQDGR
ITYTYENSKIDLRISIINTIHGQKCVIRILNPESFKIGMQNLGISENEQKIIEGFTKFENGMVLICGPTGSGKTTTLYTM
LEQENTIDKNIITVENPVEYNIFGINQIQINEKIGLRFDNTLKFVLRQDPSVIMVGEIRDLETSKLAIRASITGHKVYST
IHTDDCYQSIIRLMDMGIEDYMIRASLKVVIAQRLVRKLCPHCKKKVTPTSEQMEIFRSNGLNPTEIYEPGNCDECSLGY
DGRMAVMQILKIDDDLRSLIKKDMNLKSLKNYGEENNIDSLFKKALIQVALGNTSFDEAMRLYNED

Sequences:

>Translated_386_residues
MEINDNILFVNSIIDDAIRKKSSDIHIEPFEENVRIRFRINGSLVEYKTVDKSMHSKIITRIKIMSELDIAEKRLPQDGR
ITYTYENSKIDLRISIINTIHGQKCVIRILNPESFKIGMQNLGISENEQKIIEGFTKFENGMVLICGPTGSGKTTTLYTM
LEQENTIDKNIITVENPVEYNIFGINQIQINEKIGLRFDNTLKFVLRQDPSVIMVGEIRDLETSKLAIRASITGHKVYST
IHTDDCYQSIIRLMDMGIEDYMIRASLKVVIAQRLVRKLCPHCKKKVTPTSEQMEIFRSNGLNPTEIYEPGNCDECSLGY
DGRMAVMQILKIDDDLRSLIKKDMNLKSLKNYGEENNIDSLFKKALIQVALGNTSFDEAMRLYNED
>Mature_386_residues
MEINDNILFVNSIIDDAIRKKSSDIHIEPFEENVRIRFRINGSLVEYKTVDKSMHSKIITRIKIMSELDIAEKRLPQDGR
ITYTYENSKIDLRISIINTIHGQKCVIRILNPESFKIGMQNLGISENEQKIIEGFTKFENGMVLICGPTGSGKTTTLYTM
LEQENTIDKNIITVENPVEYNIFGINQIQINEKIGLRFDNTLKFVLRQDPSVIMVGEIRDLETSKLAIRASITGHKVYST
IHTDDCYQSIIRLMDMGIEDYMIRASLKVVIAQRLVRKLCPHCKKKVTPTSEQMEIFRSNGLNPTEIYEPGNCDECSLGY
DGRMAVMQILKIDDDLRSLIKKDMNLKSLKNYGEENNIDSLFKKALIQVALGNTSFDEAMRLYNED

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=379, Percent_Identity=41.688654353562, Blast_Score=342, Evalue=3e-95,
Organism=Escherichia coli, GI1786296, Length=376, Percent_Identity=38.2978723404255, Blast_Score=295, Evalue=3e-81,
Organism=Escherichia coli, GI87082188, Length=278, Percent_Identity=31.294964028777, Blast_Score=112, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 44223; Mature: 44223

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEINDNILFVNSIIDDAIRKKSSDIHIEPFEENVRIRFRINGSLVEYKTVDKSMHSKIIT
CCCCCCEEEEHHHHHHHHHCCCCCEEEEECCCCEEEEEEECCCEEEEEHHHHHHHHHHHH
RIKIMSELDIAEKRLPQDGRITYTYENSKIDLRISIINTIHGQKCVIRILNPESFKIGMQ
HHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEEEEEEECCCCCEEEEEEECCCCEEEEHH
NLGISENEQKIIEGFTKFENGMVLICGPTGSGKTTTLYTMLEQENTIDKNIITVENPVEY
HCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEECCCCCCCCCEEEECCCCEE
NIFGINQIQINEKIGLRFDNTLKFVLRQDPSVIMVGEIRDLETSKLAIRASITGHKVYST
EEEEEEEEEECCEECCEECCCEEEEEECCCCEEEEECCCCCCCCEEEEEEEECCCEEEEE
IHTDDCYQSIIRLMDMGIEDYMIRASLKVVIAQRLVRKLCPHCKKKVTPTSEQMEIFRSN
ECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
GLNPTEIYEPGNCDECSLGYDGRMAVMQILKIDDDLRSLIKKDMNLKSLKNYGEENNIDS
CCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCHHHHHCCCCCCCHHH
LFKKALIQVALGNTSFDEAMRLYNED
HHHHHHHHHHCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MEINDNILFVNSIIDDAIRKKSSDIHIEPFEENVRIRFRINGSLVEYKTVDKSMHSKIIT
CCCCCCEEEEHHHHHHHHHCCCCCEEEEECCCCEEEEEEECCCEEEEEHHHHHHHHHHHH
RIKIMSELDIAEKRLPQDGRITYTYENSKIDLRISIINTIHGQKCVIRILNPESFKIGMQ
HHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEEEEEEECCCCCEEEEEEECCCCEEEEHH
NLGISENEQKIIEGFTKFENGMVLICGPTGSGKTTTLYTMLEQENTIDKNIITVENPVEY
HCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEECCCCCCCCCEEEECCCCEE
NIFGINQIQINEKIGLRFDNTLKFVLRQDPSVIMVGEIRDLETSKLAIRASITGHKVYST
EEEEEEEEEECCEECCEECCCEEEEEECCCCEEEEECCCCCCCCEEEEEEEECCCEEEEE
IHTDDCYQSIIRLMDMGIEDYMIRASLKVVIAQRLVRKLCPHCKKKVTPTSEQMEIFRSN
ECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
GLNPTEIYEPGNCDECSLGYDGRMAVMQILKIDDDLRSLIKKDMNLKSLKNYGEENNIDS
CCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCHHHHHCCCCCCCHHH
LFKKALIQVALGNTSFDEAMRLYNED
HHHHHHHHHHCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]