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The map label for this gene is thiDE [H]

Identifier: 169823899

GI number: 169823899

Start: 238995

End: 239798

Strand: Direct

Name: thiDE [H]

Synonym: FMG_0202

Alternate gene names: 169823899

Gene position: 238995-239798 (Clockwise)

Preceding gene: 169823898

Following gene: 169823900

Centisome position: 13.3

GC content: 41.04

Gene sequence:

>804_bases
ATGAAGAAAGTTCTTACGATTGCAGGTTCTGATTGTTCGGGCGGCGCGGGAATTCAAGCGGATATCAAGACTATGTCAGC
ACACGGGGTGTATGCGATGAGCGTTATTGTTTCGGTGGTTGCTGAGAATACTTTTGAGGTGGTGTCGATTGAGGATTTGC
CCGATAAGATTATAAAGGATCAGATTGATTGTATTTATAATGATATGGGCACTGACGCTGTGAAGATTGGAATGTTATCG
AACAAGAGGATTATGAGTTGTGTTAGGGATAAATTATTGGAGAACAAACCAGAAAATGTGGTGTTGGATCCTGTGATGTA
TGCAAAGCACGGCGCTTGTTTGATGCAAGAAGATGCGATTGATTTGTTGATTGAGGATTTAATTGCCTTATGCACGTGCA
TAACTCCGAATATTCCAGAGGCAGAGAGAATCAGTGGAATGAAAATCGACGGTGTGGATGATATGAAAAAGGCAGCTGTG
ATTATTCGTAACATGGGAGCGAAATACGTTCTTGTGAAGGGCGGTCACCATGTGGGAGATGCGATTGATATTTTGTATGA
TGGCGAGAATTTCCACGAATTTGTGAATGAAAAGATTGACACGAAGAACACTCATGGGACTGGTTGCACGCTTTCAAGTG
CGATTGCCAGTAATTTGGCGCTTGGAAAGTCGATATATGACGCTGTAAAATCAAGCAAGGAATACATTACTAATGCTATC
AAGCATGATTTGCATATTGGCAAGGGAAATGGGCCGACAAATCATTTCTACAAATTTTTTGAAGGAGAGTTGAAAAATGA
ATAA

Upstream 100 bases:

>100_bases
AAAAAATATCTGAGATTACGAAACTCTACAACAGGGATTAGTTGAAGTAAAATGATTATAGGTATATAATTTAATTTACA
GCAAATTATTTGGAGGAGTT

Downstream 100 bases:

>100_bases
ACAATTTAACTTAGGGTTGAACTTGGTCACGAACAAACAAACTCTCAAGGGAAGGGATTTGACGGAGACTATTGAGATTG
CGCTGAAAAATGGTGCGGAT

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MKKVLTIAGSDCSGGAGIQADIKTMSAHGVYAMSVIVSVVAENTFEVVSIEDLPDKIIKDQIDCIYNDMGTDAVKIGMLS
NKRIMSCVRDKLLENKPENVVLDPVMYAKHGACLMQEDAIDLLIEDLIALCTCITPNIPEAERISGMKIDGVDDMKKAAV
IIRNMGAKYVLVKGGHHVGDAIDILYDGENFHEFVNEKIDTKNTHGTGCTLSSAIASNLALGKSIYDAVKSSKEYITNAI
KHDLHIGKGNGPTNHFYKFFEGELKNE

Sequences:

>Translated_267_residues
MKKVLTIAGSDCSGGAGIQADIKTMSAHGVYAMSVIVSVVAENTFEVVSIEDLPDKIIKDQIDCIYNDMGTDAVKIGMLS
NKRIMSCVRDKLLENKPENVVLDPVMYAKHGACLMQEDAIDLLIEDLIALCTCITPNIPEAERISGMKIDGVDDMKKAAV
IIRNMGAKYVLVKGGHHVGDAIDILYDGENFHEFVNEKIDTKNTHGTGCTLSSAIASNLALGKSIYDAVKSSKEYITNAI
KHDLHIGKGNGPTNHFYKFFEGELKNE
>Mature_267_residues
MKKVLTIAGSDCSGGAGIQADIKTMSAHGVYAMSVIVSVVAENTFEVVSIEDLPDKIIKDQIDCIYNDMGTDAVKIGMLS
NKRIMSCVRDKLLENKPENVVLDPVMYAKHGACLMQEDAIDLLIEDLIALCTCITPNIPEAERISGMKIDGVDDMKKAAV
IIRNMGAKYVLVKGGHHVGDAIDILYDGENFHEFVNEKIDTKNTHGTGCTLSSAIASNLALGKSIYDAVKSSKEYITNAI
KHDLHIGKGNGPTNHFYKFFEGELKNE

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=266, Percent_Identity=39.0977443609023, Blast_Score=182, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6325378, Length=274, Percent_Identity=36.1313868613139, Blast_Score=151, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6324997, Length=275, Percent_Identity=38.5454545454545, Blast_Score=141, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6324517, Length=275, Percent_Identity=37.4545454545455, Blast_Score=141, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 29039; Mature: 29039

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVLTIAGSDCSGGAGIQADIKTMSAHGVYAMSVIVSVVAENTFEVVSIEDLPDKIIKD
CCCEEEEECCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHH
QIDCIYNDMGTDAVKIGMLSNKRIMSCVRDKLLENKPENVVLDPVMYAKHGACLMQEDAI
HHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEECCHHHHCCCCEEEHHHHH
DLLIEDLIALCTCITPNIPEAERISGMKIDGVDDMKKAAVIIRNMGAKYVLVKGGHHVGD
HHHHHHHHHHHHHCCCCCCCHHHHCCCEECCHHHHHHHHHHHHHCCCCEEEEECCCCCCC
AIDILYDGENFHEFVNEKIDTKNTHGTGCTLSSAIASNLALGKSIYDAVKSSKEYITNAI
EEEEEECCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KHDLHIGKGNGPTNHFYKFFEGELKNE
HHHCEECCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKVLTIAGSDCSGGAGIQADIKTMSAHGVYAMSVIVSVVAENTFEVVSIEDLPDKIIKD
CCCEEEEECCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHH
QIDCIYNDMGTDAVKIGMLSNKRIMSCVRDKLLENKPENVVLDPVMYAKHGACLMQEDAI
HHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEECCHHHHCCCCEEEHHHHH
DLLIEDLIALCTCITPNIPEAERISGMKIDGVDDMKKAAVIIRNMGAKYVLVKGGHHVGD
HHHHHHHHHHHHHCCCCCCCHHHHCCCEECCHHHHHHHHHHHHHCCCCEEEEECCCCCCC
AIDILYDGENFHEFVNEKIDTKNTHGTGCTLSSAIASNLALGKSIYDAVKSSKEYITNAI
EEEEEECCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KHDLHIGKGNGPTNHFYKFFEGELKNE
HHHCEECCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA