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The map label for this gene is thiE [H]

Identifier: 169823900

GI number: 169823900

Start: 239791

End: 240429

Strand: Direct

Name: thiE [H]

Synonym: FMG_0203

Alternate gene names: 169823900

Gene position: 239791-240429 (Clockwise)

Preceding gene: 169823899

Following gene: 169823901

Centisome position: 13.34

GC content: 41.63

Gene sequence:

>639_bases
ATGAATAAACAATTTAACTTAGGGTTGAACTTGGTCACGAACAAACAAACTCTCAAGGGAAGGGATTTGACGGAGACTAT
TGAGATTGCGCTGAAAAATGGTGCGGATTCTGTTAGACTTAGAGAAAAAAACATGGACACTAGAAGCATTATGCAAGAGG
CTTTCAAGATAAAAGAAATTACTCAACGCATGGGCAAGCTTTTGATTGTGAATGACAGGGTTGATATTGCGAAGGCATGC
GATGTCGACGGAGTGCATTTGGGACAAAAGGATATGCCGATAAAATACGCGAGGGAGATGCTCGGAGACGACAAGATTAT
TGGGATTAGTTGTCACACTTTGGAACAAGCGTTAGAGGCACAAGAGGCTGGCGCTGATTACATTGGTGTGGGGGCGATTT
TTCCGACGTTCACGGGAGATGATTTCTTGATGGTTACAATCGACACGTTAAAGGAGATTTCCGAGAAGATTCACGTTCCA
ATTACTGCGATTGGCGGTATTAACAAGAACAATATTAGGATGATTTTCGACAGTAATGTGGATTCGGTGTCGTTGACATC
AGCGGTTTTTTCAACGGGAGATGTCGCAGCATCAACACAGGAGTTGAAACAAAAATTCGATATGATTTTGAAAAAATAA

Upstream 100 bases:

>100_bases
AAGGAATACATTACTAATGCTATCAAGCATGATTTGCATATTGGCAAGGGAAATGGGCCGACAAATCATTTCTACAAATT
TTTTGAAGGAGAGTTGAAAA

Downstream 100 bases:

>100_bases
TGAGAGGTGCGAGGGCACCTCTTTTTTGATGCAGTGAAGGTGCTAAGGTAGGTTAAATTCACACGAAACACAAAAACTCG
TAAACCGCTCATCTGTCTAC

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MNKQFNLGLNLVTNKQTLKGRDLTETIEIALKNGADSVRLREKNMDTRSIMQEAFKIKEITQRMGKLLIVNDRVDIAKAC
DVDGVHLGQKDMPIKYAREMLGDDKIIGISCHTLEQALEAQEAGADYIGVGAIFPTFTGDDFLMVTIDTLKEISEKIHVP
ITAIGGINKNNIRMIFDSNVDSVSLTSAVFSTGDVAASTQELKQKFDMILKK

Sequences:

>Translated_212_residues
MNKQFNLGLNLVTNKQTLKGRDLTETIEIALKNGADSVRLREKNMDTRSIMQEAFKIKEITQRMGKLLIVNDRVDIAKAC
DVDGVHLGQKDMPIKYAREMLGDDKIIGISCHTLEQALEAQEAGADYIGVGAIFPTFTGDDFLMVTIDTLKEISEKIHVP
ITAIGGINKNNIRMIFDSNVDSVSLTSAVFSTGDVAASTQELKQKFDMILKK
>Mature_212_residues
MNKQFNLGLNLVTNKQTLKGRDLTETIEIALKNGADSVRLREKNMDTRSIMQEAFKIKEITQRMGKLLIVNDRVDIAKAC
DVDGVHLGQKDMPIKYAREMLGDDKIIGISCHTLEQALEAQEAGADYIGVGAIFPTFTGDDFLMVTIDTLKEISEKIHVP
ITAIGGINKNNIRMIFDSNVDSVSLTSAVFSTGDVAASTQELKQKFDMILKK

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6325042, Length=226, Percent_Identity=33.1858407079646, Blast_Score=125, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 23450; Mature: 23450

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKQFNLGLNLVTNKQTLKGRDLTETIEIALKNGADSVRLREKNMDTRSIMQEAFKIKEI
CCCCEECCEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHH
TQRMGKLLIVNDRVDIAKACDVDGVHLGQKDMPIKYAREMLGDDKIIGISCHTLEQALEA
HHHHCCEEEEECCCCHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHH
QEAGADYIGVGAIFPTFTGDDFLMVTIDTLKEISEKIHVPITAIGGINKNNIRMIFDSNV
HHCCCCEEEECEECCCCCCCCEEEEEHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECCC
DSVSLTSAVFSTGDVAASTQELKQKFDMILKK
CCEEHHHHHHCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKQFNLGLNLVTNKQTLKGRDLTETIEIALKNGADSVRLREKNMDTRSIMQEAFKIKEI
CCCCEECCEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHH
TQRMGKLLIVNDRVDIAKACDVDGVHLGQKDMPIKYAREMLGDDKIIGISCHTLEQALEA
HHHHCCEEEEECCCCHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHH
QEAGADYIGVGAIFPTFTGDDFLMVTIDTLKEISEKIHVPITAIGGINKNNIRMIFDSNV
HHCCCCEEEECEECCCCCCCCEEEEEHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECCC
DSVSLTSAVFSTGDVAASTQELKQKFDMILKK
CCEEHHHHHHCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]