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The map label for this gene is vanS [H]

Identifier: 169823821

GI number: 169823821

Start: 142749

End: 143831

Strand: Direct

Name: vanS [H]

Synonym: FMG_0124

Alternate gene names: 169823821

Gene position: 142749-143831 (Clockwise)

Preceding gene: 169823820

Following gene: 169823822

Centisome position: 7.94

GC content: 29.27

Gene sequence:

>1083_bases
ATGCGTAAAGAAAATTCATATATTAAAAGAATCATTCTGTATCAATGTTTATGGATAGCTGTTGGAATTTTTATTACATT
TGCTTTTCATCAATTGATAGACACCTTGATAATTTACAATATCGACACAGAAGCCGAGATGAGTTCAATATATAAGATTT
ATTTTGGATATGGTCATTATTTTTTCATTATGCTAGGCGCAATTGTAATAACCATCGGATGTTTTTACATTCAGAACAAA
CAATTTGAAAAATATCGCGATTATTTTGTATCTGGAATTAAATACATTAAAGGCGAAATTCCTGAGATAAGAGAATTTCA
CGAATCTCTATCCAACGAAAGATTGGATTTCATTAATATTCGAAAACAAATTTTAGAAATGGAAAACAATTATGAACTAG
CATATAAGGAAAAAACAGATTTGCTGACGTATTTGGCACATGATATCAAAACGCCTCTATCAAATTTATTGGGATATTCA
ACGTTGCTTTACGATGAAGATAATTTAACTTATGAGCAACAAAAAAAGTTCATAAAAGTTATCTACGAAAATGTGAAATC
ACTAAACAGTTTGAGCGAGGATTTCTTCTCCTATTTGAAATTCAATTTAAATGAAATTCCAATTAACATCAAAAAGTTTG
ATGCAGAACTATTTTTAAAACAATGGGAAGAAGAAAGAAAAACACTGGTGAACGATAACGATTTGGATATTAGATTTGCA
AATACTTCTTGTAAAGAAATTAATACAGACGCTGAATTACTCGTGAGGATTTTGGATAATTTGGTATCGAACGCGACCAA
ATACTCAAAAAAGGGTACAACCATAATTGTTGAAGTTGAGATAAAGGATAAATCATTAAATTTGACGGTTAAAAATGAAA
TCGAAAATGATTTGAACGTAGATTGGAAATTGGTGAAAAATAAATTTTATCGCGGAGATATGTCACGAAATCGCATAAAA
AACAATGGCTCAGGAGTTGGACTTACAATTGTAAATGATATTGTAGCACATTTGAATGGAAAATTTGATGTGTACAATAA
AGATAATTTGGTAATTGCGAATGTAATTATTCCCGTATATTAA

Upstream 100 bases:

>100_bases
AATCCCGTTATGGTTCACATAAGAAGAATACGAGCTAAATTCGAACAATGCGGAATAAGTTCAATAATAAAGACAATATG
GGGAGTGGGGTATAAGATTA

Downstream 100 bases:

>100_bases
AAAAGAAAGGAAAAAGCAAGAATTAATACAAAAAAACTTAATCTCTTATTTTGAAGACTAACCTATAATGACTTTATAAG
GTTAGTTTTTTATTGGCAAG

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MRKENSYIKRIILYQCLWIAVGIFITFAFHQLIDTLIIYNIDTEAEMSSIYKIYFGYGHYFFIMLGAIVITIGCFYIQNK
QFEKYRDYFVSGIKYIKGEIPEIREFHESLSNERLDFINIRKQILEMENNYELAYKEKTDLLTYLAHDIKTPLSNLLGYS
TLLYDEDNLTYEQQKKFIKVIYENVKSLNSLSEDFFSYLKFNLNEIPINIKKFDAELFLKQWEEERKTLVNDNDLDIRFA
NTSCKEINTDAELLVRILDNLVSNATKYSKKGTTIIVEVEIKDKSLNLTVKNEIENDLNVDWKLVKNKFYRGDMSRNRIK
NNGSGVGLTIVNDIVAHLNGKFDVYNKDNLVIANVIIPVY

Sequences:

>Translated_360_residues
MRKENSYIKRIILYQCLWIAVGIFITFAFHQLIDTLIIYNIDTEAEMSSIYKIYFGYGHYFFIMLGAIVITIGCFYIQNK
QFEKYRDYFVSGIKYIKGEIPEIREFHESLSNERLDFINIRKQILEMENNYELAYKEKTDLLTYLAHDIKTPLSNLLGYS
TLLYDEDNLTYEQQKKFIKVIYENVKSLNSLSEDFFSYLKFNLNEIPINIKKFDAELFLKQWEEERKTLVNDNDLDIRFA
NTSCKEINTDAELLVRILDNLVSNATKYSKKGTTIIVEVEIKDKSLNLTVKNEIENDLNVDWKLVKNKFYRGDMSRNRIK
NNGSGVGLTIVNDIVAHLNGKFDVYNKDNLVIANVIIPVY
>Mature_360_residues
MRKENSYIKRIILYQCLWIAVGIFITFAFHQLIDTLIIYNIDTEAEMSSIYKIYFGYGHYFFIMLGAIVITIGCFYIQNK
QFEKYRDYFVSGIKYIKGEIPEIREFHESLSNERLDFINIRKQILEMENNYELAYKEKTDLLTYLAHDIKTPLSNLLGYS
TLLYDEDNLTYEQQKKFIKVIYENVKSLNSLSEDFFSYLKFNLNEIPINIKKFDAELFLKQWEEERKTLVNDNDLDIRFA
NTSCKEINTDAELLVRILDNLVSNATKYSKKGTTIIVEVEIKDKSLNLTVKNEIENDLNVDWKLVKNKFYRGDMSRNRIK
NNGSGVGLTIVNDIVAHLNGKFDVYNKDNLVIANVIIPVY

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=224, Percent_Identity=26.3392857142857, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1786600, Length=200, Percent_Identity=23.5, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI48994928, Length=233, Percent_Identity=21.8884120171674, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 42328; Mature: 42328

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKENSYIKRIILYQCLWIAVGIFITFAFHQLIDTLIIYNIDTEAEMSSIYKIYFGYGHY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHH
FFIMLGAIVITIGCFYIQNKQFEKYRDYFVSGIKYIKGEIPEIREFHESLSNERLDFINI
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHH
RKQILEMENNYELAYKEKTDLLTYLAHDIKTPLSNLLGYSTLLYDEDNLTYEQQKKFIKV
HHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCCCHHHHHHHHHH
IYENVKSLNSLSEDFFSYLKFNLNEIPINIKKFDAELFLKQWEEERKTLVNDNDLDIRFA
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEC
NTSCKEINTDAELLVRILDNLVSNATKYSKKGTTIIVEVEIKDKSLNLTVKNEIENDLNV
CCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCEEEEEECCCCCCCCCC
DWKLVKNKFYRGDMSRNRIKNNGSGVGLTIVNDIVAHLNGKFDVYNKDNLVIANVIIPVY
CHHHHHHHHHCCCCHHHHHCCCCCEEEHHHHHHHHHHHCCCEEEECCCCEEEEEEEEECC
>Mature Secondary Structure
MRKENSYIKRIILYQCLWIAVGIFITFAFHQLIDTLIIYNIDTEAEMSSIYKIYFGYGHY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHH
FFIMLGAIVITIGCFYIQNKQFEKYRDYFVSGIKYIKGEIPEIREFHESLSNERLDFINI
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHH
RKQILEMENNYELAYKEKTDLLTYLAHDIKTPLSNLLGYSTLLYDEDNLTYEQQKKFIKV
HHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCCCHHHHHHHHHH
IYENVKSLNSLSEDFFSYLKFNLNEIPINIKKFDAELFLKQWEEERKTLVNDNDLDIRFA
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEC
NTSCKEINTDAELLVRILDNLVSNATKYSKKGTTIIVEVEIKDKSLNLTVKNEIENDLNV
CCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCEEEEEECCCCCCCCCC
DWKLVKNKFYRGDMSRNRIKNNGSGVGLTIVNDIVAHLNGKFDVYNKDNLVIANVIIPVY
CHHHHHHHHHCCCCHHHHHCCCCCEEEHHHHHHHHHHHCCCEEEECCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]