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The map label for this gene is braB [H]

Identifier: 169823804

GI number: 169823804

Start: 128113

End: 129390

Strand: Direct

Name: braB [H]

Synonym: FMG_0107

Alternate gene names: 169823804

Gene position: 128113-129390 (Clockwise)

Preceding gene: 169823800

Following gene: 169823805

Centisome position: 7.13

GC content: 34.9

Gene sequence:

>1278_bases
ATGAAAGATAAAAAACTAATAATTGTTAGTGGGTTTGCACTATTCTCAATGTTTTTCGGAGCAGGGAATTTGATATTTCC
ACCATATCTTGGAAACAAAGTTGGCGATCAATATTTGATAGCTATGATTGGATTCTTGTTGAGTTCGGTTGGACTTGTGT
TTCTGGGATTATTGGCAGCGACAAAATGTGGAGGAACAATTGAAAAAATCGGAAATACTATAAATCCTCGATTCTCACTG
ATATTTTCAACAATAATTATGCTTGCGATTGGACCTGGACTTGCAATTCCAAGAACCGCAGCTACAACGGTAGAAGTAAT
ACAAAAAGGATTCTTCAAAGGAGCAAATTCCATTTTGCTCACAGCAATATTTTTTGTTTGTGTGTATTTATTCGTAAGAA
AACCTAACAAGATAATAGATAATTTGGGAAAAATTTTGACACCATTATTGATTGCGACTCTTCTTGCGATTATTTTCAAA
GGAATTTTATTTCCTGTCGCAACACCCGTGAATACATATCAAATTCAAGTCTTCCAATCAAGCTTTGAAGAAGGATATCA
AACAATGGATGCATTGGCATCAATTGTATTTGCAATCGTAGTTACCAAAGGATTCATGCAAAACGGAATCAAAGATGAAG
ACAAAATCAAAGATTACACGATAAAATCTGCAACTATTTCAGCCATTGGACTATGTTTTGTATATGGGGGATTATTATTC
ATCGGCGCGACAACTTCCAATCTAAATTTGGGCAACATGGAAAGAATAGAATTGCTATTGTACATTTGCAACCAACAATT
GGGAGTGTTTGGAAAAATATTAATCTGCATTGCAATGACATTGGCATGCCTCACAACAGCAATCGGACTTACGACAACTG
TAGGAGAATTTTTCGGCGATATGCTCAAAGATAAAACAAATGGAATCATGTCATACGATTTGATAATAATCATATCCACT
GTGTTCAGCGCGTTTTTTGCAGTAGTCGGAGTGGAAAAAATCTTGAAAATATCCGCGCCAATACTTTCAGCAATCTATCC
AGTAGCAATCGTGTTAATATTCTTGAACCTTTTCAACAAAGATACATTCGAAAAAAACACGAAACTCGGAGCAATCATAG
GCGCATCAATTTTTGGATTTTTAATTCTACTCAACAGCTTCAAATTGGATTTTGGCTTGTTGAAATTAATCGAATCCGTA
TTTCCAATGCAAATCAAAAGCTTCATTTGGATAATTCCAGCAGTTATTGGCGGATTAATAGGAAAAATCAAAAAATAG

Upstream 100 bases:

>100_bases
CACAAGTTTGTTTTTTAGGAAAAATTGTTGTTTATTGGACAAAATTGTGATAGAATTGTAAGAAATGATAAAAATTTAAA
ATAATTATACAGAGGTAAAG

Downstream 100 bases:

>100_bases
ACCAAATTGTAACTATTATGTAATACGATATATAGTGCAATGTATGGTATAATATCGTTGAGGTGAGTAAATGATCAAAA
AAATATTTGGCTTGAAAAAC

Product: branched-chain amino acid transport system carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BraB [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MKDKKLIIVSGFALFSMFFGAGNLIFPPYLGNKVGDQYLIAMIGFLLSSVGLVFLGLLAATKCGGTIEKIGNTINPRFSL
IFSTIIMLAIGPGLAIPRTAATTVEVIQKGFFKGANSILLTAIFFVCVYLFVRKPNKIIDNLGKILTPLLIATLLAIIFK
GILFPVATPVNTYQIQVFQSSFEEGYQTMDALASIVFAIVVTKGFMQNGIKDEDKIKDYTIKSATISAIGLCFVYGGLLF
IGATTSNLNLGNMERIELLLYICNQQLGVFGKILICIAMTLACLTTAIGLTTTVGEFFGDMLKDKTNGIMSYDLIIIIST
VFSAFFAVVGVEKILKISAPILSAIYPVAIVLIFLNLFNKDTFEKNTKLGAIIGASIFGFLILLNSFKLDFGLLKLIESV
FPMQIKSFIWIIPAVIGGLIGKIKK

Sequences:

>Translated_425_residues
MKDKKLIIVSGFALFSMFFGAGNLIFPPYLGNKVGDQYLIAMIGFLLSSVGLVFLGLLAATKCGGTIEKIGNTINPRFSL
IFSTIIMLAIGPGLAIPRTAATTVEVIQKGFFKGANSILLTAIFFVCVYLFVRKPNKIIDNLGKILTPLLIATLLAIIFK
GILFPVATPVNTYQIQVFQSSFEEGYQTMDALASIVFAIVVTKGFMQNGIKDEDKIKDYTIKSATISAIGLCFVYGGLLF
IGATTSNLNLGNMERIELLLYICNQQLGVFGKILICIAMTLACLTTAIGLTTTVGEFFGDMLKDKTNGIMSYDLIIIIST
VFSAFFAVVGVEKILKISAPILSAIYPVAIVLIFLNLFNKDTFEKNTKLGAIIGASIFGFLILLNSFKLDFGLLKLIESV
FPMQIKSFIWIIPAVIGGLIGKIKK
>Mature_425_residues
MKDKKLIIVSGFALFSMFFGAGNLIFPPYLGNKVGDQYLIAMIGFLLSSVGLVFLGLLAATKCGGTIEKIGNTINPRFSL
IFSTIIMLAIGPGLAIPRTAATTVEVIQKGFFKGANSILLTAIFFVCVYLFVRKPNKIIDNLGKILTPLLIATLLAIIFK
GILFPVATPVNTYQIQVFQSSFEEGYQTMDALASIVFAIVVTKGFMQNGIKDEDKIKDYTIKSATISAIGLCFVYGGLLF
IGATTSNLNLGNMERIELLLYICNQQLGVFGKILICIAMTLACLTTAIGLTTTVGEFFGDMLKDKTNGIMSYDLIIIIST
VFSAFFAVVGVEKILKISAPILSAIYPVAIVLIFLNLFNKDTFEKNTKLGAIIGASIFGFLILLNSFKLDFGLLKLIESV
FPMQIKSFIWIIPAVIGGLIGKIKK

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=423, Percent_Identity=29.3144208037825, Blast_Score=187, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 46107; Mature: 46107

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDKKLIIVSGFALFSMFFGAGNLIFPPYLGNKVGDQYLIAMIGFLLSSVGLVFLGLLAA
CCCCCEEEEEHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TKCGGTIEKIGNTINPRFSLIFSTIIMLAIGPGLAIPRTAATTVEVIQKGFFKGANSILL
HHCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHH
TAIFFVCVYLFVRKPNKIIDNLGKILTPLLIATLLAIIFKGILFPVATPVNTYQIQVFQS
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
SFEEGYQTMDALASIVFAIVVTKGFMQNGIKDEDKIKDYTIKSATISAIGLCFVYGGLLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHEEHHHHHHHHHHHHHHHHHHH
IGATTSNLNLGNMERIELLLYICNQQLGVFGKILICIAMTLACLTTAIGLTTTVGEFFGD
EECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLKDKTNGIMSYDLIIIISTVFSAFFAVVGVEKILKISAPILSAIYPVAIVLIFLNLFNK
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DTFEKNTKLGAIIGASIFGFLILLNSFKLDFGLLKLIESVFPMQIKSFIWIIPAVIGGLI
CHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GKIKK
HHCCC
>Mature Secondary Structure
MKDKKLIIVSGFALFSMFFGAGNLIFPPYLGNKVGDQYLIAMIGFLLSSVGLVFLGLLAA
CCCCCEEEEEHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TKCGGTIEKIGNTINPRFSLIFSTIIMLAIGPGLAIPRTAATTVEVIQKGFFKGANSILL
HHCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHH
TAIFFVCVYLFVRKPNKIIDNLGKILTPLLIATLLAIIFKGILFPVATPVNTYQIQVFQS
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
SFEEGYQTMDALASIVFAIVVTKGFMQNGIKDEDKIKDYTIKSATISAIGLCFVYGGLLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHEEHHHHHHHHHHHHHHHHHHH
IGATTSNLNLGNMERIELLLYICNQQLGVFGKILICIAMTLACLTTAIGLTTTVGEFFGD
EECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLKDKTNGIMSYDLIIIISTVFSAFFAVVGVEKILKISAPILSAIYPVAIVLIFLNLFNK
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DTFEKNTKLGAIIGASIFGFLILLNSFKLDFGLLKLIESVFPMQIKSFIWIIPAVIGGLI
CHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GKIKK
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]