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The map label for this gene is dacF [H]

Identifier: 169823800

GI number: 169823800

Start: 123484

End: 124668

Strand: Direct

Name: dacF [H]

Synonym: FMG_0103

Alternate gene names: 169823800

Gene position: 123484-124668 (Clockwise)

Preceding gene: 169823799

Following gene: 169823804

Centisome position: 6.87

GC content: 33.84

Gene sequence:

>1185_bases
ATGAAGAGTATTAAAAAAATAATACCCTTAATGTTAGTACTTGCTATTGTATTTCAACCTGCATTATCAAAAGCGGACTC
GAAGTTGGGATTTGAAGACAAAGTAAAAGCATATTACACAATTGAACAGGATTATTCCCAAGAATTGTTGAATTACAACG
AGAACAAGCCTTTGGCTATAGCATCCTTAACTAAAATCATGACTTATGTTTTGGTTATGGAAGATATCAACGCAAACAAA
TACAAACTCGACGATAAAATCAAAATCCAAACACAATATGCTAAGCCTGAAGCAAGCACAATGCAACTTAAAAAAGGCGA
AGAATTAACTGTAAATGATTTGATAAGAGGAATGATGATAGTCAGTGGCAATGATGCCGCAGACGAATTAGCAGTGAAAT
CAGAAGGATCTGTGGGAAAATTTGTCGAAAGAATGAACAAAAAAGCAGCCGAATTGGAATTGGAATCTGCGAAGTTTTAT
TCTGCATCAGGTTATCCTGAAAACGACAAGGAAAATTTGATGAGCGCAAAAGATTTGGCGAAATTAACAACTTATACAAT
CAACAATTACCCACAAGTTTTGAATTACACAAAAACACAAAAACTTGTAATGCCAAGTAGAAAATACGAAGCTGCAACTA
CAATTCCATTTTTAGGAAAAATAGAAGGCGTAGATGGATTTAAAACAGGAACAACTGAAAAAGCTGGATACTGTTTGATA
ACTACGATGAGTTTGAAACCAAACAATCCTAAAAACAAAGTAGTATCTGTTTTGATGGGAGCTCCAACAAAATACGACAG
AAATACTTACCAAAAAATAATGCTAGAATACGTCAGAAATTCATTCGAGCCAATGAATATATTAAACAGTGAAAAACCAA
TCGAAACGAAATCAGTCGACAGTGTCGTTGATAAAAAAGTTGAGATTTATCCTACAGAAGACGTATCTATTTTGTATAAT
AATAAAACTGGTCTGCAACAAAAAGTCGACATCAAAAAAGATTTGAAAGCTCCTTTGAAAAAAGGACAAGTCGTAGGGAA
AGTTATAATTGACACTGGAAAAGGCGACAAGAGAGAAGTAAGCCTTGTTGTCAAGAAACCTTACGAAAAAGCAGACACAT
TAACTAGAATAAAAAGAAGCGCCAAATACACTTACGAATTATTAGAAGCACTTCTTGCATCATAA

Upstream 100 bases:

>100_bases
AAATATCACACGATATCAAAGAAGTATATTCCGAAGAAAATATTACAGATGATGTAATAAAAGTGTACGAAGAAGTTATA
TCGAAAAGGTGAGGTGAGAA

Downstream 100 bases:

>100_bases
GCCGATTTAATTCGGTTTTTATGTGCTTCCATAATTAAGCTGGATATAATACCCCCCTCCTAAGGGGGCATCCCCGGTTC
GAATCCGGGTGGGAGTACCA

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MKSIKKIIPLMLVLAIVFQPALSKADSKLGFEDKVKAYYTIEQDYSQELLNYNENKPLAIASLTKIMTYVLVMEDINANK
YKLDDKIKIQTQYAKPEASTMQLKKGEELTVNDLIRGMMIVSGNDAADELAVKSEGSVGKFVERMNKKAAELELESAKFY
SASGYPENDKENLMSAKDLAKLTTYTINNYPQVLNYTKTQKLVMPSRKYEAATTIPFLGKIEGVDGFKTGTTEKAGYCLI
TTMSLKPNNPKNKVVSVLMGAPTKYDRNTYQKIMLEYVRNSFEPMNILNSEKPIETKSVDSVVDKKVEIYPTEDVSILYN
NKTGLQQKVDIKKDLKAPLKKGQVVGKVIIDTGKGDKREVSLVVKKPYEKADTLTRIKRSAKYTYELLEALLAS

Sequences:

>Translated_394_residues
MKSIKKIIPLMLVLAIVFQPALSKADSKLGFEDKVKAYYTIEQDYSQELLNYNENKPLAIASLTKIMTYVLVMEDINANK
YKLDDKIKIQTQYAKPEASTMQLKKGEELTVNDLIRGMMIVSGNDAADELAVKSEGSVGKFVERMNKKAAELELESAKFY
SASGYPENDKENLMSAKDLAKLTTYTINNYPQVLNYTKTQKLVMPSRKYEAATTIPFLGKIEGVDGFKTGTTEKAGYCLI
TTMSLKPNNPKNKVVSVLMGAPTKYDRNTYQKIMLEYVRNSFEPMNILNSEKPIETKSVDSVVDKKVEIYPTEDVSILYN
NKTGLQQKVDIKKDLKAPLKKGQVVGKVIIDTGKGDKREVSLVVKKPYEKADTLTRIKRSAKYTYELLEALLAS
>Mature_394_residues
MKSIKKIIPLMLVLAIVFQPALSKADSKLGFEDKVKAYYTIEQDYSQELLNYNENKPLAIASLTKIMTYVLVMEDINANK
YKLDDKIKIQTQYAKPEASTMQLKKGEELTVNDLIRGMMIVSGNDAADELAVKSEGSVGKFVERMNKKAAELELESAKFY
SASGYPENDKENLMSAKDLAKLTTYTINNYPQVLNYTKTQKLVMPSRKYEAATTIPFLGKIEGVDGFKTGTTEKAGYCLI
TTMSLKPNNPKNKVVSVLMGAPTKYDRNTYQKIMLEYVRNSFEPMNILNSEKPIETKSVDSVVDKKVEIYPTEDVSILYN
NKTGLQQKVDIKKDLKAPLKKGQVVGKVIIDTGKGDKREVSLVVKKPYEKADTLTRIKRSAKYTYELLEALLAS

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=274, Percent_Identity=29.1970802919708, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1787062, Length=354, Percent_Identity=28.2485875706215, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1786851, Length=318, Percent_Identity=28.3018867924528, Blast_Score=92, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 44282; Mature: 44282

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSIKKIIPLMLVLAIVFQPALSKADSKLGFEDKVKAYYTIEQDYSQELLNYNENKPLAI
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHCCCCCCCCHH
ASLTKIMTYVLVMEDINANKYKLDDKIKIQTQYAKPEASTMQLKKGEELTVNDLIRGMMI
HHHHHHHHHHHHHHCCCCCEEECCCEEEEEEEECCCCCHHHHCCCCCCCCHHHHHCCEEE
VSGNDAADELAVKSEGSVGKFVERMNKKAAELELESAKFYSASGYPENDKENLMSAKDLA
EECCCCHHHHEECCCCCHHHHHHHHCCHHHHEEECCCCEEECCCCCCCCHHHHHHHHHHH
KLTTYTINNYPQVLNYTKTQKLVMPSRKYEAATTIPFLGKIEGVDGFKTGTTEKAGYCLI
HHHHHCCCCCHHHHCCCCHHHEECCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEE
TTMSLKPNNPKNKVVSVLMGAPTKYDRNTYQKIMLEYVRNSFEPMNILNSEKPIETKSVD
EEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHH
SVVDKKVEIYPTEDVSILYNNKTGLQQKVDIKKDLKAPLKKGQVVGKVIIDTGKGDKREV
HHHCCCEEEEECCCEEEEECCCCCCCHHHCHHHHHHHHHHCCCEEEEEEEECCCCCCEEE
SLVVKKPYEKADTLTRIKRSAKYTYELLEALLAS
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKSIKKIIPLMLVLAIVFQPALSKADSKLGFEDKVKAYYTIEQDYSQELLNYNENKPLAI
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHCCCCCCCCHH
ASLTKIMTYVLVMEDINANKYKLDDKIKIQTQYAKPEASTMQLKKGEELTVNDLIRGMMI
HHHHHHHHHHHHHHCCCCCEEECCCEEEEEEEECCCCCHHHHCCCCCCCCHHHHHCCEEE
VSGNDAADELAVKSEGSVGKFVERMNKKAAELELESAKFYSASGYPENDKENLMSAKDLA
EECCCCHHHHEECCCCCHHHHHHHHCCHHHHEEECCCCEEECCCCCCCCHHHHHHHHHHH
KLTTYTINNYPQVLNYTKTQKLVMPSRKYEAATTIPFLGKIEGVDGFKTGTTEKAGYCLI
HHHHHCCCCCHHHHCCCCHHHEECCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEE
TTMSLKPNNPKNKVVSVLMGAPTKYDRNTYQKIMLEYVRNSFEPMNILNSEKPIETKSVD
EEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHH
SVVDKKVEIYPTEDVSILYNNKTGLQQKVDIKKDLKAPLKKGQVVGKVIIDTGKGDKREV
HHHCCCEEEEECCCEEEEECCCCCCCHHHCHHHHHHHHHHCCCEEEEEEEECCCCCCEEE
SLVVKKPYEKADTLTRIKRSAKYTYELLEALLAS
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]