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The map label for this gene is argG

Identifier: 167625995

GI number: 167625995

Start: 4977749

End: 4978987

Strand: Reverse

Name: argG

Synonym: Shal_4090

Alternate gene names: 167625995

Gene position: 4978987-4977749 (Counterclockwise)

Preceding gene: 167625996

Following gene: 167625994

Centisome position: 95.26

GC content: 47.38

Gene sequence:

>1239_bases
ATGTCCAATGAAAATTCAGCAGTAACTAAGCATGCAGGTGTTAAAAAGGTTGTTTTAGCCTATTCCGGGGGCTTAGATAC
TTCAGCAATTATCCCGTGGCTGAAGGAAACCTATGATGACTGTGAGATTATCGCCTTCTGCGCCGATGTAGGCCAAGGTG
ATGCTGAACTAGAAGGCTTGTATGAGAAAGCCATCGCTTCAGGCGCTTCGGAGTGTCATATCGTTGACTTGAAAGAAGAG
CTGGTGGCGGATTATATCTACCCAACAATCGCCACAGGCGCTATCTATGAGGGAACCTATTTATTGGGGACCTCTATGGC
GAGACCAATTATTGCTAAGGCGCAGGTTGAGGTGGCACGTAAAGTGGGCGCAGATGCAGTTTGTCATGGTTGTACCGGTA
AAGGTAATGATCAGGTTCGTTTTGAAGGTTGTTTTGCGGCATTAGCGCCAGACTTAAAAGTGATTGCACCTTGGCGTGAA
TGGGAGATGGTTAGCCGTGAAGATCTGCTCGACTATCTAGCCGAGCGAAATATTGCGACTTCTGCTTCAGCCACTAAGAT
CTATAGCCGCGATGCTAACGCTTGGCATATTTCTCACGAAGGCGGTGAGTTAGAAGATCCATGGAACGAGCCATCAAAAG
GTGTATGGACCATGACGGTTGCGCCAGAAGATGCGCCTAATCAGCCTGAATATGTGGCACTCGAGCTTGAGCAGGGCAAG
ATCACCAAAGTAAATGGTGAGGCCTTATCACCCTATAACGCACTGATGCTACTAAACGAAGTTGCGGGTGCTCATGGTGT
CGGCAGAATCGATATCACCGAAAACAGGCTCGTGGGTATGAAGTCTCGTGGCTGTTATGAAACACCGGGCGGCACAGTAA
TGTTTGCGGCGCTAAGAGCGATTGAAGAGCTAGTACTAGATAAAACGAGTCGTGAGTGGCGTGAGCAAATTGGCACGAAG
ATGGCGCACCTTGTTTATGACGGTCGTTGGTTTACCCCATTATGTGAGTCACTGCTTGGTGCCTCAAAGCCATTAGCGGA
TTTAATTAACGGTGAAGTGGTGGTTAAGCTTTATAAAGGCCAAGCCAGTGTAGTGAAGAAGCGTTCACCAAATAGCTTGT
ACTCAGAAGCGTTTGCGACATTTGGTGCCGATGATGTTTACAACCAAAAAGATGCCGAAGGTTTTATCCGTTTGTATTCG
TTATCAAGCCGGATCAGAGCACTGAATAACCAAAAGTAG

Upstream 100 bases:

>100_bases
GAACGCCGTTCTGGTAACCCTTTTAAGTTAATTTTTTAAGAAAAAAAACAGTCAACTTGCGTTAGTGAGTTGAGTAATAA
ATAAGTAGTAGGAAATTAAG

Downstream 100 bases:

>100_bases
GCTATTAACTAGCTGAAGTTAGTGAACGCTAACATAATAGCGTTAGCGCTGTATAAACAGAAAGATAAAAGGTGGGAGTT
ATCCCACCTAGCATTAGAGA

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MSNENSAVTKHAGVKKVVLAYSGGLDTSAIIPWLKETYDDCEIIAFCADVGQGDAELEGLYEKAIASGASECHIVDLKEE
LVADYIYPTIATGAIYEGTYLLGTSMARPIIAKAQVEVARKVGADAVCHGCTGKGNDQVRFEGCFAALAPDLKVIAPWRE
WEMVSREDLLDYLAERNIATSASATKIYSRDANAWHISHEGGELEDPWNEPSKGVWTMTVAPEDAPNQPEYVALELEQGK
ITKVNGEALSPYNALMLLNEVAGAHGVGRIDITENRLVGMKSRGCYETPGGTVMFAALRAIEELVLDKTSREWREQIGTK
MAHLVYDGRWFTPLCESLLGASKPLADLINGEVVVKLYKGQASVVKKRSPNSLYSEAFATFGADDVYNQKDAEGFIRLYS
LSSRIRALNNQK

Sequences:

>Translated_412_residues
MSNENSAVTKHAGVKKVVLAYSGGLDTSAIIPWLKETYDDCEIIAFCADVGQGDAELEGLYEKAIASGASECHIVDLKEE
LVADYIYPTIATGAIYEGTYLLGTSMARPIIAKAQVEVARKVGADAVCHGCTGKGNDQVRFEGCFAALAPDLKVIAPWRE
WEMVSREDLLDYLAERNIATSASATKIYSRDANAWHISHEGGELEDPWNEPSKGVWTMTVAPEDAPNQPEYVALELEQGK
ITKVNGEALSPYNALMLLNEVAGAHGVGRIDITENRLVGMKSRGCYETPGGTVMFAALRAIEELVLDKTSREWREQIGTK
MAHLVYDGRWFTPLCESLLGASKPLADLINGEVVVKLYKGQASVVKKRSPNSLYSEAFATFGADDVYNQKDAEGFIRLYS
LSSRIRALNNQK
>Mature_411_residues
SNENSAVTKHAGVKKVVLAYSGGLDTSAIIPWLKETYDDCEIIAFCADVGQGDAELEGLYEKAIASGASECHIVDLKEEL
VADYIYPTIATGAIYEGTYLLGTSMARPIIAKAQVEVARKVGADAVCHGCTGKGNDQVRFEGCFAALAPDLKVIAPWREW
EMVSREDLLDYLAERNIATSASATKIYSRDANAWHISHEGGELEDPWNEPSKGVWTMTVAPEDAPNQPEYVALELEQGKI
TKVNGEALSPYNALMLLNEVAGAHGVGRIDITENRLVGMKSRGCYETPGGTVMFAALRAIEELVLDKTSREWREQIGTKM
AHLVYDGRWFTPLCESLLGASKPLADLINGEVVVKLYKGQASVVKKRSPNSLYSEAFATFGADDVYNQKDAEGFIRLYSL
SSRIRALNNQK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=395, Percent_Identity=44.8101265822785, Blast_Score=317, Evalue=9e-87,
Organism=Homo sapiens, GI16950633, Length=395, Percent_Identity=44.8101265822785, Blast_Score=317, Evalue=9e-87,
Organism=Escherichia coli, GI1789563, Length=412, Percent_Identity=27.4271844660194, Blast_Score=140, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6324514, Length=412, Percent_Identity=40.7766990291262, Blast_Score=302, Evalue=6e-83,
Organism=Drosophila melanogaster, GI21358151, Length=407, Percent_Identity=40.5405405405405, Blast_Score=291, Evalue=6e-79,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_SHEHH (B0TL85)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001676289.1
- ProteinModelPortal:   B0TL85
- SMR:   B0TL85
- GeneID:   5902889
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_4090
- HOGENOM:   HBG335267
- OMA:   PLIAKRQ
- ProtClustDB:   PRK00509
- BioCyc:   SHAL458817:SHAL_4090-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 44998; Mature: 44867

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 48-48 BINDING 100-100 BINDING 105-105 BINDING 130-130 BINDING 132-132 BINDING 136-136 BINDING 136-136 BINDING 137-137 BINDING 140-140 BINDING 189-189 BINDING 198-198 BINDING 274-274 BINDING 286-286

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNENSAVTKHAGVKKVVLAYSGGLDTSAIIPWLKETYDDCEIIAFCADVGQGDAELEGL
CCCCCCCHHHHCCCEEEEEEECCCCCHHHHHHHHHHCCCCHHEEEEEHHCCCCHHHHHHH
YEKAIASGASECHIVDLKEELVADYIYPTIATGAIYEGTYLLGTSMARPIIAKAQVEVAR
HHHHHHCCCCCEEEEECHHHHHHHHHHHHHHCCCEECCEEEECCHHHCHHHHHHHHHHHH
KVGADAVCHGCTGKGNDQVRFEGCFAALAPDLKVIAPWREWEMVSREDLLDYLAERNIAT
HCCCHHEEECCCCCCCCCEEEEHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCC
SASATKIYSRDANAWHISHEGGELEDPWNEPSKGVWTMTVAPEDAPNQPEYVALELEQGK
CCCHHHHEECCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCE
ITKVNGEALSPYNALMLLNEVAGAHGVGRIDITENRLVGMKSRGCYETPGGTVMFAALRA
EEEECCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCEECCCCCCCCCCCCCHHHHHHHHH
IEELVLDKTSREWREQIGTKMAHLVYDGRWFTPLCESLLGASKPLADLINGEVVVKLYKG
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHCCEEEEEEECC
QASVVKKRSPNSLYSEAFATFGADDVYNQKDAEGFIRLYSLSSRIRALNNQK
CHHHHHCCCCCHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SNENSAVTKHAGVKKVVLAYSGGLDTSAIIPWLKETYDDCEIIAFCADVGQGDAELEGL
CCCCCCHHHHCCCEEEEEEECCCCCHHHHHHHHHHCCCCHHEEEEEHHCCCCHHHHHHH
YEKAIASGASECHIVDLKEELVADYIYPTIATGAIYEGTYLLGTSMARPIIAKAQVEVAR
HHHHHHCCCCCEEEEECHHHHHHHHHHHHHHCCCEECCEEEECCHHHCHHHHHHHHHHHH
KVGADAVCHGCTGKGNDQVRFEGCFAALAPDLKVIAPWREWEMVSREDLLDYLAERNIAT
HCCCHHEEECCCCCCCCCEEEEHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCC
SASATKIYSRDANAWHISHEGGELEDPWNEPSKGVWTMTVAPEDAPNQPEYVALELEQGK
CCCHHHHEECCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCE
ITKVNGEALSPYNALMLLNEVAGAHGVGRIDITENRLVGMKSRGCYETPGGTVMFAALRA
EEEECCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCEECCCCCCCCCCCCCHHHHHHHHH
IEELVLDKTSREWREQIGTKMAHLVYDGRWFTPLCESLLGASKPLADLINGEVVVKLYKG
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHCCEEEEEEECC
QASVVKKRSPNSLYSEAFATFGADDVYNQKDAEGFIRLYSLSSRIRALNNQK
CHHHHHCCCCCHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA