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The map label for this gene is argF [H]

Identifier: 167625996

GI number: 167625996

Start: 4979057

End: 4979962

Strand: Reverse

Name: argF [H]

Synonym: Shal_4091

Alternate gene names: 167625996

Gene position: 4979962-4979057 (Counterclockwise)

Preceding gene: 167625997

Following gene: 167625995

Centisome position: 95.28

GC content: 44.7

Gene sequence:

>906_bases
ATGAAACATTTACTATCAATAAAAGATTTAAGCCAGCAGCAGTTACTCGACCTTTTAGCATTGGCAAAAACCATAAAGGC
TAACCCTGCTGAATATAAACATGCCCTAGAGGGCAAGAGTGTGGTGATGCTATTTGAAAAACCCTCACTACGAACTCGGG
TGAGCTTTGATATCGGTATCAATAAATTGGGTGGTCATTGCTTATATCTAGATCAGCAAAATGGCGAGTTAGGTAAGCGT
GAACCTGTTTCAGATTTTGCTGCCAATATCTCTTGCTGGGCAGATGCGATTGTTGCGAGGACTTTCTTACACTCGACAAT
TGAAGAGCTTGCTGAGCACGGCACGGTGCCAGTGATTAACGCCTTGTCTGATCTGTACCATCCTTGTCAGGGGTTAGCGG
ACTTTCTGACATTGAGCGAACAATATGGCGATGTGAGTAAGGTGAAGCTGGCTTATGTCGGTGATGGTAACAATGTGACT
CATTCATTGATGTTTGGTGCAGCTATCTTAGGTGCGCAGTTAACCGTTATTTGTCCGCCAGGCCACTTTCCCGATGGCAA
GGTGGTTTGTGAAGCACAAGAACTGGCTGCGAAGCACGGTGGCAAACTCATTCTGAGTTCCGATGTTGAAGCGATTATTG
GTCATGATGCGATTTATGCCGACACTTGGCTCTCAATGGGAGATTCGACACCAATGGCAGAGATTGAAGCAAAGTTTAAA
CCTTACCAAGTCAATTCCCAGTTAATGCAAAAGGCAGGTGCTAAACACTTTATGCATTGTTTACCTGCGTACCGAGAAGT
TGAAGTTACCGCAGAAGTTGTTGATGGCGAAGGCTCACTTATTTTGCATCAAGCCGAAAACAGAATGCATGCGCAGAACG
CCGTTCTGGTAACCCTTTTAAGTTAA

Upstream 100 bases:

>100_bases
TGCCTCTTGGCGTCATGCGCAGCAATTAATCGCTTTAAGTAGGGGTGAGACCGTTGGCACCCAGATCCAGCCACAAATTC
AGTAATTGGAGAGCAGTCGA

Downstream 100 bases:

>100_bases
TTTTTTAAGAAAAAAAACAGTCAACTTGCGTTAGTGAGTTGAGTAATAAATAAGTAGTAGGAAATTAAGATGTCCAATGA
AAATTCAGCAGTAACTAAGC

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MKHLLSIKDLSQQQLLDLLALAKTIKANPAEYKHALEGKSVVMLFEKPSLRTRVSFDIGINKLGGHCLYLDQQNGELGKR
EPVSDFAANISCWADAIVARTFLHSTIEELAEHGTVPVINALSDLYHPCQGLADFLTLSEQYGDVSKVKLAYVGDGNNVT
HSLMFGAAILGAQLTVICPPGHFPDGKVVCEAQELAAKHGGKLILSSDVEAIIGHDAIYADTWLSMGDSTPMAEIEAKFK
PYQVNSQLMQKAGAKHFMHCLPAYREVEVTAEVVDGEGSLILHQAENRMHAQNAVLVTLLS

Sequences:

>Translated_301_residues
MKHLLSIKDLSQQQLLDLLALAKTIKANPAEYKHALEGKSVVMLFEKPSLRTRVSFDIGINKLGGHCLYLDQQNGELGKR
EPVSDFAANISCWADAIVARTFLHSTIEELAEHGTVPVINALSDLYHPCQGLADFLTLSEQYGDVSKVKLAYVGDGNNVT
HSLMFGAAILGAQLTVICPPGHFPDGKVVCEAQELAAKHGGKLILSSDVEAIIGHDAIYADTWLSMGDSTPMAEIEAKFK
PYQVNSQLMQKAGAKHFMHCLPAYREVEVTAEVVDGEGSLILHQAENRMHAQNAVLVTLLS
>Mature_301_residues
MKHLLSIKDLSQQQLLDLLALAKTIKANPAEYKHALEGKSVVMLFEKPSLRTRVSFDIGINKLGGHCLYLDQQNGELGKR
EPVSDFAANISCWADAIVARTFLHSTIEELAEHGTVPVINALSDLYHPCQGLADFLTLSEQYGDVSKVKLAYVGDGNNVT
HSLMFGAAILGAQLTVICPPGHFPDGKVVCEAQELAAKHGGKLILSSDVEAIIGHDAIYADTWLSMGDSTPMAEIEAKFK
PYQVNSQLMQKAGAKHFMHCLPAYREVEVTAEVVDGEGSLILHQAENRMHAQNAVLVTLLS

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=305, Percent_Identity=39.672131147541, Blast_Score=215, Evalue=3e-56,
Organism=Homo sapiens, GI18105007, Length=307, Percent_Identity=28.9902280130293, Blast_Score=101, Evalue=9e-22,
Organism=Escherichia coli, GI1790703, Length=327, Percent_Identity=36.085626911315, Blast_Score=197, Evalue=7e-52,
Organism=Escherichia coli, GI1786469, Length=326, Percent_Identity=35.5828220858896, Blast_Score=191, Evalue=7e-50,
Organism=Escherichia coli, GI2367364, Length=309, Percent_Identity=29.4498381877023, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI48994908, Length=301, Percent_Identity=25.5813953488372, Blast_Score=65, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6322373, Length=310, Percent_Identity=32.258064516129, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6322331, Length=192, Percent_Identity=28.125, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24642586, Length=307, Percent_Identity=24.1042345276873, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 32727; Mature: 32727

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHLLSIKDLSQQQLLDLLALAKTIKANPAEYKHALEGKSVVMLFEKPSLRTRVSFDIGI
CCCCCCHHHCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEEECCCCCEEEEEECCC
NKLGGHCLYLDQQNGELGKREPVSDFAANISCWADAIVARTFLHSTIEELAEHGTVPVIN
CCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
ALSDLYHPCQGLADFLTLSEQYGDVSKVKLAYVGDGNNVTHSLMFGAAILGAQLTVICPP
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHCCEEEEEECC
GHFPDGKVVCEAQELAAKHGGKLILSSDVEAIIGHDAIYADTWLSMGDSTPMAEIEAKFK
CCCCCCCEEEEHHHHHHHCCCEEEEECCCHHHHCCCHHHHHHHHHCCCCCCHHHHHHCCC
PYQVNSQLMQKAGAKHFMHCLPAYREVEVTAEVVDGEGSLILHQAENRMHAQNAVLVTLL
CEECCHHHHHHHCHHHHHHHHCCHHEEEEEEEEECCCCCEEEEECCCCHHHCCEEEEEEE
S
C
>Mature Secondary Structure
MKHLLSIKDLSQQQLLDLLALAKTIKANPAEYKHALEGKSVVMLFEKPSLRTRVSFDIGI
CCCCCCHHHCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEEECCCCCEEEEEECCC
NKLGGHCLYLDQQNGELGKREPVSDFAANISCWADAIVARTFLHSTIEELAEHGTVPVIN
CCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
ALSDLYHPCQGLADFLTLSEQYGDVSKVKLAYVGDGNNVTHSLMFGAAILGAQLTVICPP
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHCCEEEEEECC
GHFPDGKVVCEAQELAAKHGGKLILSSDVEAIIGHDAIYADTWLSMGDSTPMAEIEAKFK
CCCCCCCEEEEHHHHHHHCCCEEEEECCCHHHHCCCHHHHHHHHHCCCCCCHHHHHHCCC
PYQVNSQLMQKAGAKHFMHCLPAYREVEVTAEVVDGEGSLILHQAENRMHAQNAVLVTLL
CEECCHHHHHHHCHHHHHHHHCCHHEEEEEEEEECCCCCEEEEECCCCHHHCCEEEEEEE
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]