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The map label for this gene is rep [H]

Identifier: 167625835

GI number: 167625835

Start: 4788862

End: 4790877

Strand: Reverse

Name: rep [H]

Synonym: Shal_3930

Alternate gene names: 167625835

Gene position: 4790877-4788862 (Counterclockwise)

Preceding gene: 167625844

Following gene: 167625821

Centisome position: 91.66

GC content: 46.23

Gene sequence:

>2016_bases
ATGAAGCTCAATCCCGGACAAAATGACGCCGTTCACTATGTTAGCGGCCCTTGCTTAGTACTTGCAGGCGCAGGCAGTGG
CAAAACCAGAGTAATTATTAATAAAATTGCCTATCTGGTGGAAAAGTGTGGCTATAAAGCACGCAATATCGCAGCGGTAA
CCTTTACCAATAAAGCGGCGCGCGAGATGAAAGAGCGTGTGTCGCAGTCTATGGGACGTAAAGAAGCACGTGGTTTGTGG
ATCTCAACTTTCCATACCTTAGGCCTTGAGATCATTAAGCGTGAACATAAAGTTGTGGGCTTAAAGCCCGGCTTCTCACT
ATTTGACGACCAAGATACCTTTGCACTGCTAAAAGAGCTGACTCAAGATGAGTTAGACGAAGATAAAGACTTACTCAAGT
TATTGGCCAGTACCATTTCTAATTGGAAAGGCGATCTTGTTATTCCTGAGCAAGCTCGAAAAATTGCTAAAGACGAGCAA
CAGCAACTCTTTGCTTTGTTGTATCAGCGTTATGCTCAGCATATGAAGGCCTATAATGCCCTCGACTTCGATGATTTGAT
CTTATTACCGACTTTGTTACTTAGGCAAAATGAAGAGGTGAGAACTCGCTGGCAAACCCGTATTCAATATATGCTGGTGG
ATGAATATCAAGACACTAACACCAGTCAGTATGAGATGGTTAAGTTATTGGTTGGTGAACGGGCGCGTTTTACCGTGGTA
GGTGATGACGATCAGTCAATCTACTCTTGGCGTGGTGCTAAGCCGCAAAACTTAGTATTACTCGGCAAAGATTTTCCTAG
ATTAAAGTTGATTAAGCTGGAGCAAAATTATCGTTCTAGCCAGCGAATTCTGCGTGCCGCAAACATCCTCATCGCCAATA
ACCCACATGTGTACGAAAAAGCACTGTTTAGTGAGCTCGCTTATGGTGAGCCCATTAGGGTATTGATTGCTGCTAACGAG
GAGCAAGAAGCGGAGCGGGTGATTGCGGAAATGATCCGCCATAAGTTTGTTGGTCGAACAAGCTATGGTGATTACGCCAT
TTTATATCGAGGCAATCACCAGTCGCGCTTACTCGAACGGGCTTTGATGACTAACCGTATCCCTTATAAGCTCAGCGGAG
GCACCTCTTTCTTTGGCCGCGCAGAGATTAAAGACATCATGGCGTACCTGCGTTTAGTGGTAAACCCTGATGATGACAAT
GCATTTTTGCGAATTGTTAACCTACCTAAGCGGGGAATTGGTCCGGCAACGCTTGAGCGTTTAGGTAACTTTGCTAATAT
GAAGCAGATCTCCATGTTTGCCGCGATCTTTGAAGCGGATCTGAATCATGAACTGCCGCCTGCGGCGATGTCATCTTTGT
ATCAGTTTGGCCGTTTTGTGGTTGAAATGGGTGACTGTGCCGAGCGTGGTGATCCCGTGGAAGCGGTTAAGCAGTTAATT
AGAAAGATTAACTACGAAGACTATCTTTACGAAAACAGTACTAGCGCTAAAGCGGCTGAAATGCGCATGAAGAACATTTC
AGAACTGTACCGCTGGGTGACAGAGATGCTTGAAGGTGATGAGCTCGATGAAGGTATGACACTACCTGAGGTGGTCACTC
GTCTGACATTGCGCGACATGATGGAACGTAATAGTGAAGATGAGGGCGGCGATCAAGTACAGTTAATGACACTGCACGCC
TCTAAAGGCCTAGAGTTTCCTTATGTGTTTATGGTGGGCGCCGAAGAGCGGATTTTGCCGCACCAAAGCAGTATCGACGA
AGATAATGTCGATGAGGAACGTCGTTTAGCCTATGTGGGGATCACTCGCGCGCAAAAAGAGTTGTGGTTTGTGATCTGCC
GCGAGCGACGCCAGTTTGGTGAAACCATCCGCTGCGAGCCGAGCCGTTTCTTGATGGAACTACCACAAGATGACTTGATC
TGGGAGAACCGCAAACCGGTGCAAACCGTAGAGCAGCGTCAGCAATCAGGCAAAGCCAATATTGCCAACTTAAGAGATAT
GTTTAAGAACAAATAA

Upstream 100 bases:

>100_bases
CACAGTCGAGATATTGTCGATAACTTTTCCGCTTGCTAGGGGCTTTCTGCTATCATTCCTCTTTTAATGCCTTCCTTAAT
ACCTTGGTTATAACGTATCC

Downstream 100 bases:

>100_bases
TCCTTAAGCTATACCTGAGCTAGGCTAAACTTAAGTGTGTGCCCTATTGAAGTTAGGCACACACACTATTTTTTTTTGTT
CTAGCTCAATGACATTGCCT

Product: ATP-dependent DNA helicase Rep

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 671; Mature: 671

Protein sequence:

>671_residues
MKLNPGQNDAVHYVSGPCLVLAGAGSGKTRVIINKIAYLVEKCGYKARNIAAVTFTNKAAREMKERVSQSMGRKEARGLW
ISTFHTLGLEIIKREHKVVGLKPGFSLFDDQDTFALLKELTQDELDEDKDLLKLLASTISNWKGDLVIPEQARKIAKDEQ
QQLFALLYQRYAQHMKAYNALDFDDLILLPTLLLRQNEEVRTRWQTRIQYMLVDEYQDTNTSQYEMVKLLVGERARFTVV
GDDDQSIYSWRGAKPQNLVLLGKDFPRLKLIKLEQNYRSSQRILRAANILIANNPHVYEKALFSELAYGEPIRVLIAANE
EQEAERVIAEMIRHKFVGRTSYGDYAILYRGNHQSRLLERALMTNRIPYKLSGGTSFFGRAEIKDIMAYLRLVVNPDDDN
AFLRIVNLPKRGIGPATLERLGNFANMKQISMFAAIFEADLNHELPPAAMSSLYQFGRFVVEMGDCAERGDPVEAVKQLI
RKINYEDYLYENSTSAKAAEMRMKNISELYRWVTEMLEGDELDEGMTLPEVVTRLTLRDMMERNSEDEGGDQVQLMTLHA
SKGLEFPYVFMVGAEERILPHQSSIDEDNVDEERRLAYVGITRAQKELWFVICRERRQFGETIRCEPSRFLMELPQDDLI
WENRKPVQTVEQRQQSGKANIANLRDMFKNK

Sequences:

>Translated_671_residues
MKLNPGQNDAVHYVSGPCLVLAGAGSGKTRVIINKIAYLVEKCGYKARNIAAVTFTNKAAREMKERVSQSMGRKEARGLW
ISTFHTLGLEIIKREHKVVGLKPGFSLFDDQDTFALLKELTQDELDEDKDLLKLLASTISNWKGDLVIPEQARKIAKDEQ
QQLFALLYQRYAQHMKAYNALDFDDLILLPTLLLRQNEEVRTRWQTRIQYMLVDEYQDTNTSQYEMVKLLVGERARFTVV
GDDDQSIYSWRGAKPQNLVLLGKDFPRLKLIKLEQNYRSSQRILRAANILIANNPHVYEKALFSELAYGEPIRVLIAANE
EQEAERVIAEMIRHKFVGRTSYGDYAILYRGNHQSRLLERALMTNRIPYKLSGGTSFFGRAEIKDIMAYLRLVVNPDDDN
AFLRIVNLPKRGIGPATLERLGNFANMKQISMFAAIFEADLNHELPPAAMSSLYQFGRFVVEMGDCAERGDPVEAVKQLI
RKINYEDYLYENSTSAKAAEMRMKNISELYRWVTEMLEGDELDEGMTLPEVVTRLTLRDMMERNSEDEGGDQVQLMTLHA
SKGLEFPYVFMVGAEERILPHQSSIDEDNVDEERRLAYVGITRAQKELWFVICRERRQFGETIRCEPSRFLMELPQDDLI
WENRKPVQTVEQRQQSGKANIANLRDMFKNK
>Mature_671_residues
MKLNPGQNDAVHYVSGPCLVLAGAGSGKTRVIINKIAYLVEKCGYKARNIAAVTFTNKAAREMKERVSQSMGRKEARGLW
ISTFHTLGLEIIKREHKVVGLKPGFSLFDDQDTFALLKELTQDELDEDKDLLKLLASTISNWKGDLVIPEQARKIAKDEQ
QQLFALLYQRYAQHMKAYNALDFDDLILLPTLLLRQNEEVRTRWQTRIQYMLVDEYQDTNTSQYEMVKLLVGERARFTVV
GDDDQSIYSWRGAKPQNLVLLGKDFPRLKLIKLEQNYRSSQRILRAANILIANNPHVYEKALFSELAYGEPIRVLIAANE
EQEAERVIAEMIRHKFVGRTSYGDYAILYRGNHQSRLLERALMTNRIPYKLSGGTSFFGRAEIKDIMAYLRLVVNPDDDN
AFLRIVNLPKRGIGPATLERLGNFANMKQISMFAAIFEADLNHELPPAAMSSLYQFGRFVVEMGDCAERGDPVEAVKQLI
RKINYEDYLYENSTSAKAAEMRMKNISELYRWVTEMLEGDELDEGMTLPEVVTRLTLRDMMERNSEDEGGDQVQLMTLHA
SKGLEFPYVFMVGAEERILPHQSSIDEDNVDEERRLAYVGITRAQKELWFVICRERRQFGETIRCEPSRFLMELPQDDLI
WENRKPVQTVEQRQQSGKANIANLRDMFKNK

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=671, Percent_Identity=68.5543964232489, Blast_Score=932, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=641, Percent_Identity=38.5335413416537, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1787196, Length=346, Percent_Identity=28.6127167630058, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322369, Length=438, Percent_Identity=32.4200913242009, Blast_Score=204, Evalue=4e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77244; Mature: 77244

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNPGQNDAVHYVSGPCLVLAGAGSGKTRVIINKIAYLVEKCGYKARNIAAVTFTNKAA
CCCCCCCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHH
REMKERVSQSMGRKEARGLWISTFHTLGLEIIKREHKVVGLKPGFSLFDDQDTFALLKEL
HHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHH
TQDELDEDKDLLKLLASTISNWKGDLVIPEQARKIAKDEQQQLFALLYQRYAQHMKAYNA
HHHHCCCHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LDFDDLILLPTLLLRQNEEVRTRWQTRIQYMLVDEYQDTNTSQYEMVKLLVGERARFTVV
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHCCCCEEEEE
GDDDQSIYSWRGAKPQNLVLLGKDFPRLKLIKLEQNYRSSQRILRAANILIANNPHVYEK
CCCCCHHHHCCCCCCCCEEEECCCCCCEEEEEEHHHHHHHHHHHHHHCEEEECCCHHHHH
ALFSELAYGEPIRVLIAANEEQEAERVIAEMIRHKFVGRTSYGDYAILYRGNHQSRLLER
HHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHH
ALMTNRIPYKLSGGTSFFGRAEIKDIMAYLRLVVNPDDDNAFLRIVNLPKRGIGPATLER
HHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHEECCCCCCEEEEEEECCCCCCCHHHHHH
LGNFANMKQISMFAAIFEADLNHELPPAAMSSLYQFGRFVVEMGDCAERGDPVEAVKQLI
HCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHH
RKINYEDYLYENSTSAKAAEMRMKNISELYRWVTEMLEGDELDEGMTLPEVVTRLTLRDM
HHCCCHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
MERNSEDEGGDQVQLMTLHASKGLEFPYVFMVGAEERILPHQSSIDEDNVDEERRLAYVG
HHCCCCCCCCCEEEEEEEECCCCCCCCEEEEECCCHHCCCCCCCCCCCCCCHHHEEEEEE
ITRAQKELWFVICRERRQFGETIRCEPSRFLMELPQDDLIWENRKPVQTVEQRQQSGKAN
HHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCC
IANLRDMFKNK
HHHHHHHHCCC
>Mature Secondary Structure
MKLNPGQNDAVHYVSGPCLVLAGAGSGKTRVIINKIAYLVEKCGYKARNIAAVTFTNKAA
CCCCCCCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHH
REMKERVSQSMGRKEARGLWISTFHTLGLEIIKREHKVVGLKPGFSLFDDQDTFALLKEL
HHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHH
TQDELDEDKDLLKLLASTISNWKGDLVIPEQARKIAKDEQQQLFALLYQRYAQHMKAYNA
HHHHCCCHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LDFDDLILLPTLLLRQNEEVRTRWQTRIQYMLVDEYQDTNTSQYEMVKLLVGERARFTVV
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHCCCCEEEEE
GDDDQSIYSWRGAKPQNLVLLGKDFPRLKLIKLEQNYRSSQRILRAANILIANNPHVYEK
CCCCCHHHHCCCCCCCCEEEECCCCCCEEEEEEHHHHHHHHHHHHHHCEEEECCCHHHHH
ALFSELAYGEPIRVLIAANEEQEAERVIAEMIRHKFVGRTSYGDYAILYRGNHQSRLLER
HHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHH
ALMTNRIPYKLSGGTSFFGRAEIKDIMAYLRLVVNPDDDNAFLRIVNLPKRGIGPATLER
HHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHEECCCCCCEEEEEEECCCCCCCHHHHHH
LGNFANMKQISMFAAIFEADLNHELPPAAMSSLYQFGRFVVEMGDCAERGDPVEAVKQLI
HCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHH
RKINYEDYLYENSTSAKAAEMRMKNISELYRWVTEMLEGDELDEGMTLPEVVTRLTLRDM
HHCCCHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
MERNSEDEGGDQVQLMTLHASKGLEFPYVFMVGAEERILPHQSSIDEDNVDEERRLAYVG
HHCCCCCCCCCEEEEEEEECCCCCCCCEEEEECCCHHCCCCCCCCCCCCCCHHHEEEEEE
ITRAQKELWFVICRERRQFGETIRCEPSRFLMELPQDDLIWENRKPVQTVEQRQQSGKAN
HHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCC
IANLRDMFKNK
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]