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The map label for this gene is epsE [H]

Identifier: 167625755

GI number: 167625755

Start: 4670748

End: 4672481

Strand: Reverse

Name: epsE [H]

Synonym: Shal_3850

Alternate gene names: 167625755

Gene position: 4672481-4670748 (Counterclockwise)

Preceding gene: 167625756

Following gene: 167625754

Centisome position: 89.39

GC content: 47.98

Gene sequence:

>1734_bases
GTGAAACCCAAGTTAAAAATGCGATTAGGTGATCTGCTTGTTCAAGAGCACATCATCACCGAAGATCAATTACAACAAGC
ATTGAGTGAACAGCGTAATACGGGAAAGAAACTTGGGCGCACCCTAATTGACTTGTCATGTATCACAGAACAGCAATTAC
TGCAGTTCTTGTCGCATCAGCTCAACATTCCTTTCATCGATATTAGTAAACGTGCCATCGCTACAGAAGTGGTTAGTCTG
CTTCCAGAGGTACAGGCTCGTCGTTATCGTGCCCTAGTGGTTGAAGACAACGGCGACAGTGTGCTGGTTGCAATGAGCGA
TCCTGCCGATCTTCAGGCGATGGATCATCTTGAGCTGCAGTTGGCTCCTAAGAGTATTTCTATTGCCGTTGTGACTGAAG
AGCAGTTATTGCACGCGTTTGATAACCTCTATCGACGTACCGACGAGATAGCTGAAATTGCAGGTAGGCTTGAAGAGGAA
TATGCCGCCGATGATCTGTTTGATCTCGGGAGCCTGACCGACGGTGATAGCGACAACGAAACCACCGTTGTTAAGCTACT
GCAGTCCATCTTTGAAGATGCGATACAGATGCGAGCCTCAGATATTCATATTGAGCCCGGTGAAAAGGCGTTGCGAATTC
GCCAGCGTATCGATGGCCAGTTACACGAAAATATTTTGCCCGAAGTCAGTATCGCTGCAGCGTTAGTATTGCGCTTAAAG
TTGATGGCAGGCTTGGATATTTCAGAGAAACGTTTACCACAAGATGGCCGTTTTCACATGGAAATTAAAGGCCATAAGAT
TGACGTGCGTATGTCGACGATGCCTATTTACCATGGCGAATCAGTGGTGATGCGTTTGCTGGACCAATCCGCAGGACTGC
TTACCTTGAACGAAACGGGTATGCCGCCACAAATGCTGGCTAGGATCCGCCGCCAGATCAAGCGTCCTCACGGTATGTTA
CTGGTAACTGGGCCAACGGGTAGCGGTAAAACCACCACGCTTTACGGTGTACTCAGTGAGCTCAATACCGCAGATCGCAA
GATCATTACAGTTGAAGACCCTGTTGAGTATCAGTTACCTCGTATTAACCAGGTACAAGTTAACCATAAGATCGGTCTCG
ACTTTTCTAATGTGTTAAGAACCACCTTACGTCAAGATCCGGACATTATCATGGTCGGTGAGATGCGTGACCAAGAGACG
GTAGAAATCGGTCTTCGAGGCGCATTAACCGGTCACTTCGTACTGTCTACTTTACATACAAACGATGCGGTGACCAGTGC
GCTACGTCTGTTAGACATGGGGGCGGCGAGTTACTTGGTTGCTAGTGCGCTTAGGGTCATTATTGCCCAGCGACTGGTAC
GCCGAGTATGTCAAAATTGTGCGATTGACTACTATCTCAATCCACAGGATAAAGTCTGGTTATCCAGCGTGAGCCAGCTC
GATTACTCACAAGCAACATTTAAGATCGGTACTGGCTGCCAAAGCTGTAATGGCAGTGGTTACCGCGGACGTATTGGTGT
GTTTGAGATGTTAGAGCTTGATGAGCCCATGATTGAGGCTATGCGAGGTGGAAACCCGCAAGACTTTACCCGCGCCGCTT
ATCAGAGTCCAAACTTTACTCCGCTTGCAAAGTCGGCCCTGAAATATTTAGCGCAGGGCATGACGACCATCGAAGAGGTC
GCCAAGTTGGTTGAAGATGTCAGCGATAATTCATTAGATTTGGGTGAGGCTTAG

Upstream 100 bases:

>100_bases
AGGCGAAATTGGCGTATAACCAAGCTTTGTCACAAGGTAATTTGTCTGCACAGGCTAAAGTTTACGTCGATAATCGATTG
CTGCAGTTAGGAGCGTACTA

Downstream 100 bases:

>100_bases
GCATGCCTACATATCAGTACCGCGGTCGAAGCGCTCAAGGCGAGCAGATAAAGGGGCAAGTCGATGCGAGCTCAGAGAGC
GCGGCTGCCGATCAACTGAT

Product: type II secretion system protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MKPKLKMRLGDLLVQEHIITEDQLQQALSEQRNTGKKLGRTLIDLSCITEQQLLQFLSHQLNIPFIDISKRAIATEVVSL
LPEVQARRYRALVVEDNGDSVLVAMSDPADLQAMDHLELQLAPKSISIAVVTEEQLLHAFDNLYRRTDEIAEIAGRLEEE
YAADDLFDLGSLTDGDSDNETTVVKLLQSIFEDAIQMRASDIHIEPGEKALRIRQRIDGQLHENILPEVSIAAALVLRLK
LMAGLDISEKRLPQDGRFHMEIKGHKIDVRMSTMPIYHGESVVMRLLDQSAGLLTLNETGMPPQMLARIRRQIKRPHGML
LVTGPTGSGKTTTLYGVLSELNTADRKIITVEDPVEYQLPRINQVQVNHKIGLDFSNVLRTTLRQDPDIIMVGEMRDQET
VEIGLRGALTGHFVLSTLHTNDAVTSALRLLDMGAASYLVASALRVIIAQRLVRRVCQNCAIDYYLNPQDKVWLSSVSQL
DYSQATFKIGTGCQSCNGSGYRGRIGVFEMLELDEPMIEAMRGGNPQDFTRAAYQSPNFTPLAKSALKYLAQGMTTIEEV
AKLVEDVSDNSLDLGEA

Sequences:

>Translated_577_residues
MKPKLKMRLGDLLVQEHIITEDQLQQALSEQRNTGKKLGRTLIDLSCITEQQLLQFLSHQLNIPFIDISKRAIATEVVSL
LPEVQARRYRALVVEDNGDSVLVAMSDPADLQAMDHLELQLAPKSISIAVVTEEQLLHAFDNLYRRTDEIAEIAGRLEEE
YAADDLFDLGSLTDGDSDNETTVVKLLQSIFEDAIQMRASDIHIEPGEKALRIRQRIDGQLHENILPEVSIAAALVLRLK
LMAGLDISEKRLPQDGRFHMEIKGHKIDVRMSTMPIYHGESVVMRLLDQSAGLLTLNETGMPPQMLARIRRQIKRPHGML
LVTGPTGSGKTTTLYGVLSELNTADRKIITVEDPVEYQLPRINQVQVNHKIGLDFSNVLRTTLRQDPDIIMVGEMRDQET
VEIGLRGALTGHFVLSTLHTNDAVTSALRLLDMGAASYLVASALRVIIAQRLVRRVCQNCAIDYYLNPQDKVWLSSVSQL
DYSQATFKIGTGCQSCNGSGYRGRIGVFEMLELDEPMIEAMRGGNPQDFTRAAYQSPNFTPLAKSALKYLAQGMTTIEEV
AKLVEDVSDNSLDLGEA
>Mature_577_residues
MKPKLKMRLGDLLVQEHIITEDQLQQALSEQRNTGKKLGRTLIDLSCITEQQLLQFLSHQLNIPFIDISKRAIATEVVSL
LPEVQARRYRALVVEDNGDSVLVAMSDPADLQAMDHLELQLAPKSISIAVVTEEQLLHAFDNLYRRTDEIAEIAGRLEEE
YAADDLFDLGSLTDGDSDNETTVVKLLQSIFEDAIQMRASDIHIEPGEKALRIRQRIDGQLHENILPEVSIAAALVLRLK
LMAGLDISEKRLPQDGRFHMEIKGHKIDVRMSTMPIYHGESVVMRLLDQSAGLLTLNETGMPPQMLARIRRQIKRPHGML
LVTGPTGSGKTTTLYGVLSELNTADRKIITVEDPVEYQLPRINQVQVNHKIGLDFSNVLRTTLRQDPDIIMVGEMRDQET
VEIGLRGALTGHFVLSTLHTNDAVTSALRLLDMGAASYLVASALRVIIAQRLVRRVCQNCAIDYYLNPQDKVWLSSVSQL
DYSQATFKIGTGCQSCNGSGYRGRIGVFEMLELDEPMIEAMRGGNPQDFTRAAYQSPNFTPLAKSALKYLAQGMTTIEEV
AKLVEDVSDNSLDLGEA

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=410, Percent_Identity=45.1219512195122, Blast_Score=328, Evalue=8e-91,
Organism=Escherichia coli, GI1786296, Length=483, Percent_Identity=39.7515527950311, Blast_Score=310, Evalue=2e-85,
Organism=Escherichia coli, GI87082188, Length=197, Percent_Identity=39.5939086294416, Blast_Score=118, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 64262; Mature: 64262

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPKLKMRLGDLLVQEHIITEDQLQQALSEQRNTGKKLGRTLIDLSCITEQQLLQFLSHQ
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
LNIPFIDISKRAIATEVVSLLPEVQARRYRALVVEDNGDSVLVAMSDPADLQAMDHLELQ
CCCCEEECCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEEECCCCCHHHHCCCEEE
LAPKSISIAVVTEEQLLHAFDNLYRRTDEIAEIAGRLEEEYAADDLFDLGSLTDGDSDNE
ECCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
TTVVKLLQSIFEDAIQMRASDIHIEPGEKALRIRQRIDGQLHENILPEVSIAAALVLRLK
HHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHH
LMAGLDISEKRLPQDGRFHMEIKGHKIDVRMSTMPIYHGESVVMRLLDQSAGLLTLNETG
HHHCCCCHHHHCCCCCCEEEEEECCEEEEEEEEECEECCHHHHHHHHHCCCCEEEECCCC
MPPQMLARIRRQIKRPHGMLLVTGPTGSGKTTTLYGVLSELNTADRKIITVEDPVEYQLP
CCHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCC
RINQVQVNHKIGLDFSNVLRTTLRQDPDIIMVGEMRDQETVEIGLRGALTGHFVLSTLHT
CCCEEEECCEECCCHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCC
NDAVTSALRLLDMGAASYLVASALRVIIAQRLVRRVCQNCAIDYYLNPQDKVWLSSVSQL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHC
DYSQATFKIGTGCQSCNGSGYRGRIGVFEMLELDEPMIEAMRGGNPQDFTRAAYQSPNFT
CCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHCCCHHHHHHHHHCCCCCC
PLAKSALKYLAQGMTTIEEVAKLVEDVSDNSLDLGEA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKPKLKMRLGDLLVQEHIITEDQLQQALSEQRNTGKKLGRTLIDLSCITEQQLLQFLSHQ
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
LNIPFIDISKRAIATEVVSLLPEVQARRYRALVVEDNGDSVLVAMSDPADLQAMDHLELQ
CCCCEEECCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEEECCCCCHHHHCCCEEE
LAPKSISIAVVTEEQLLHAFDNLYRRTDEIAEIAGRLEEEYAADDLFDLGSLTDGDSDNE
ECCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
TTVVKLLQSIFEDAIQMRASDIHIEPGEKALRIRQRIDGQLHENILPEVSIAAALVLRLK
HHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHH
LMAGLDISEKRLPQDGRFHMEIKGHKIDVRMSTMPIYHGESVVMRLLDQSAGLLTLNETG
HHHCCCCHHHHCCCCCCEEEEEECCEEEEEEEEECEECCHHHHHHHHHCCCCEEEECCCC
MPPQMLARIRRQIKRPHGMLLVTGPTGSGKTTTLYGVLSELNTADRKIITVEDPVEYQLP
CCHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCC
RINQVQVNHKIGLDFSNVLRTTLRQDPDIIMVGEMRDQETVEIGLRGALTGHFVLSTLHT
CCCEEEECCEECCCHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCC
NDAVTSALRLLDMGAASYLVASALRVIIAQRLVRRVCQNCAIDYYLNPQDKVWLSSVSQL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHC
DYSQATFKIGTGCQSCNGSGYRGRIGVFEMLELDEPMIEAMRGGNPQDFTRAAYQSPNFT
CCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHCCCHHHHHHHHHCCCCCC
PLAKSALKYLAQGMTTIEEVAKLVEDVSDNSLDLGEA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]