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The map label for this gene is mltF [H]

Identifier: 167625642

GI number: 167625642

Start: 4534142

End: 4535080

Strand: Reverse

Name: mltF [H]

Synonym: Shal_3737

Alternate gene names: 167625642

Gene position: 4535080-4534142 (Counterclockwise)

Preceding gene: 167625643

Following gene: 167625641

Centisome position: 86.76

GC content: 45.05

Gene sequence:

>939_bases
ATGAGACTTCAAGTGAAATCAAGGTTTCTAATCCAGCTATTATTATTCTTTATTCTTGGCGCCTCGATGACTCAAGTCAT
GGCAAGGGATTTGGCCGAGATACAAGCCGAAGGGGTGTTACGCCACCTTGGCGTACCCTATGCAAACTTTGTGTCTCAGT
ACTCTGAAGGCAACAAGCTAACCCATAGCGGCCTCGATATTGAACTAATGCAAAATTTTGCCCGTTACCTAGGTGTCGAG
TATGAGTTTGTGCCTGCCACTTGGACAAGCGTCTTCGGTATGCTGACTGGGCGTAATGGTCAGTTTATTAATAATAAAGT
GGTATATGGTGAGCCGGAGTCAATTATTGGTGATGTGATTGCCAATGGTTTTACAATCTTTGATTGGCGCAAGGAGATTG
TCGATTTTTCTGATGATTACTTCCCGTCAGCTGTCTGGCTGGTGGCGCGCACCGAATCAAATCTCAACCCTATCACTCCC
AGTGGTTCAACCCTAAGAGATGTTCGTATGGTGAAGGGGATGCTGAAAGAGCGTGATATATTGGCGATGAAACAATCCTG
CCTAGATCCTGACCTTTATAATCTATATGCAACTCAGGCGAATGTTATCTTGCCAACTAAGCAGCTGCAGCTCAATGAGA
TGGTACCGTTTCTCTTAAACGTTGAGGCTGAGGCGACCCTATTAGACGTCGCGGATTCGCTGATAGCGATAGATAAGTGG
CCAAGTGCCATCAAGATTATCGGCCCCATTTCAGACGAGCAAAGAATGGCTGTCGGTTTCCGTAAACAGTCTCCCGAGCT
ACGAAAAGCCTTTAACCAGTATCTAAGGCAGATTCGTGCTGATGGCAGCTATAACAAGCTAGTGAAGAAGTATTACCCGA
CAGTTTTTCATTACTATCAAGACTATTTTGATAAATCAGCGGCAGGTGTTCACAAGTAA

Upstream 100 bases:

>100_bases
TTAAAAAGTGTAATCTGAGTCGTTGAAATTACATCGGCTCAGGGTATACTCCACGGCCGTCATTCAAGCTGAACGCTCCC
AAAGAAGTGAGACTTTTACG

Downstream 100 bases:

>100_bases
CATGAAACACTTTAAGTGGTTTAACAGTAAAACAGCGATTATCGTTGCAGGCATTCTATTGAGTGCCTACTTGATCCTTA
TTCTGACGGTTACTAACTTA

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: Murein lyase F [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MRLQVKSRFLIQLLLFFILGASMTQVMARDLAEIQAEGVLRHLGVPYANFVSQYSEGNKLTHSGLDIELMQNFARYLGVE
YEFVPATWTSVFGMLTGRNGQFINNKVVYGEPESIIGDVIANGFTIFDWRKEIVDFSDDYFPSAVWLVARTESNLNPITP
SGSTLRDVRMVKGMLKERDILAMKQSCLDPDLYNLYATQANVILPTKQLQLNEMVPFLLNVEAEATLLDVADSLIAIDKW
PSAIKIIGPISDEQRMAVGFRKQSPELRKAFNQYLRQIRADGSYNKLVKKYYPTVFHYYQDYFDKSAAGVHK

Sequences:

>Translated_312_residues
MRLQVKSRFLIQLLLFFILGASMTQVMARDLAEIQAEGVLRHLGVPYANFVSQYSEGNKLTHSGLDIELMQNFARYLGVE
YEFVPATWTSVFGMLTGRNGQFINNKVVYGEPESIIGDVIANGFTIFDWRKEIVDFSDDYFPSAVWLVARTESNLNPITP
SGSTLRDVRMVKGMLKERDILAMKQSCLDPDLYNLYATQANVILPTKQLQLNEMVPFLLNVEAEATLLDVADSLIAIDKW
PSAIKIIGPISDEQRMAVGFRKQSPELRKAFNQYLRQIRADGSYNKLVKKYYPTVFHYYQDYFDKSAAGVHK
>Mature_312_residues
MRLQVKSRFLIQLLLFFILGASMTQVMARDLAEIQAEGVLRHLGVPYANFVSQYSEGNKLTHSGLDIELMQNFARYLGVE
YEFVPATWTSVFGMLTGRNGQFINNKVVYGEPESIIGDVIANGFTIFDWRKEIVDFSDDYFPSAVWLVARTESNLNPITP
SGSTLRDVRMVKGMLKERDILAMKQSCLDPDLYNLYATQANVILPTKQLQLNEMVPFLLNVEAEATLLDVADSLIAIDKW
PSAIKIIGPISDEQRMAVGFRKQSPELRKAFNQYLRQIRADGSYNKLVKKYYPTVFHYYQDYFDKSAAGVHK

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family [H]

Homologues:

None

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR001638
- InterPro:   IPR000189 [H]

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT [H]

EC number: NA

Molecular weight: Translated: 35523; Mature: 35523

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLQVKSRFLIQLLLFFILGASMTQVMARDLAEIQAEGVLRHLGVPYANFVSQYSEGNKL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCE
THSGLDIELMQNFARYLGVEYEFVPATWTSVFGMLTGRNGQFINNKVVYGEPESIIGDVI
ECCCCCHHHHHHHHHHHCCCEEEECHHHHHHHHHHCCCCCCEECCEEEECCCHHHHHHHH
ANGFTIFDWRKEIVDFSDDYFPSAVWLVARTESNLNPITPSGSTLRDVRMVKGMLKERDI
HCCCEEEHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LAMKQSCLDPDLYNLYATQANVILPTKQLQLNEMVPFLLNVEAEATLLDVADSLIAIDKW
HHHHHHCCCCHHHHHHHCCCCEEECCHHHHHHHHCHHHCCCCCHHHHHHHHHHHHHHCCC
PSAIKIIGPISDEQRMAVGFRKQSPELRKAFNQYLRQIRADGSYNKLVKKYYPTVFHYYQ
CCCEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
DYFDKSAAGVHK
HHHCCCCCCCCC
>Mature Secondary Structure
MRLQVKSRFLIQLLLFFILGASMTQVMARDLAEIQAEGVLRHLGVPYANFVSQYSEGNKL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCE
THSGLDIELMQNFARYLGVEYEFVPATWTSVFGMLTGRNGQFINNKVVYGEPESIIGDVI
ECCCCCHHHHHHHHHHHCCCEEEECHHHHHHHHHHCCCCCCEECCEEEECCCHHHHHHHH
ANGFTIFDWRKEIVDFSDDYFPSAVWLVARTESNLNPITPSGSTLRDVRMVKGMLKERDI
HCCCEEEHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LAMKQSCLDPDLYNLYATQANVILPTKQLQLNEMVPFLLNVEAEATLLDVADSLIAIDKW
HHHHHHCCCCHHHHHHHCCCCEEECCHHHHHHHHCHHHCCCCCHHHHHHHHHHHHHHCCC
PSAIKIIGPISDEQRMAVGFRKQSPELRKAFNQYLRQIRADGSYNKLVKKYYPTVFHYYQ
CCCEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
DYFDKSAAGVHK
HHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA