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The map label for this gene is rlmB [H]

Identifier: 167625583

GI number: 167625583

Start: 4478216

End: 4478956

Strand: Reverse

Name: rlmB [H]

Synonym: Shal_3677

Alternate gene names: 167625583

Gene position: 4478956-4478216 (Counterclockwise)

Preceding gene: 167625584

Following gene: 167625581

Centisome position: 85.69

GC content: 49.66

Gene sequence:

>741_bases
ATGAAAAAGCAAGATATGACCTTTGGGTTACACGCGGTTGAAGCCGTACTTAAAAACAGTCCTGAGCGTGTCATTGAGAT
GTGGGTGCTTCAAGGTCGTGATGACGCGAGGCTCACTCCAATTCTTGCAATGGCAGCTGAGTGGGGCGTGTCAGTGCAGT
ATGCTTCTCGCAAGGCGTTAGACGAAAAGTCTGACGGCGGTCAACACCAAGGTGTCGTTGCCAAGGTTAAGCCTGCGAAA
ATTCTTAGTGATAATGAATTGTCTGCAATGCTAGAAAAGACTGAAGTGCCTTTCTTGCTAATCTTAGATGGTGTTACCGA
CCCGCATAACCTAGGGGCATGTTTACGTAATGCCGATGCAGCTGGCGTACATGGTGTGATTGTTCCTAAAGATAACTCTG
TTGGCTTAACACCAGTCGTGAGCAAAGTGGCTTGCGGCGCAGCAGAAGTGGTACCGCTTTATCAAGTGACTAACCTTGCA
CGTACTATGCGTAGCCTGCAAGAGAAGGGCATTTGGATTATTGGCGCAGCCGGTGAAGCAGATTGTGAGCTTTATCAAGC
GAGTCTAACGGGTCCTCTTGCTATCGCAATGGGCGCAGAAGGCAAAGGTCTTCGCCGCTTATCGCGTGAAAGCTGTGATA
CTTTGGTATCGATTCCTATGGCGGGTAGCGTGTCGAGCTTAAACGTTTCAGTTGCGACTGGCGTTTGCTTGTTTGAAGCG
GTGCGCCAGCGCCTAGCGTAA

Upstream 100 bases:

>100_bases
CAGCAGCGCTGCAGCTAAAAAGACTAAGACAAAAGCTTCGGCAAAAAAATCTTCAGCAAGTAAAGCTAAGTCAGCCAAGC
GTAAAGCGAGTAAGAAATAG

Downstream 100 bases:

>100_bases
TGAATGAGAGGGATGCGCTTCGGACTTGAGTTTTGCACTTGATTTCGGAGTTGGCATTAACTTTAAAATGCCGATAGGGT
TTAACCTTATCGGCATTTTT

Product: 23S rRNA (guanosine-2'-O-)-methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MKKQDMTFGLHAVEAVLKNSPERVIEMWVLQGRDDARLTPILAMAAEWGVSVQYASRKALDEKSDGGQHQGVVAKVKPAK
ILSDNELSAMLEKTEVPFLLILDGVTDPHNLGACLRNADAAGVHGVIVPKDNSVGLTPVVSKVACGAAEVVPLYQVTNLA
RTMRSLQEKGIWIIGAAGEADCELYQASLTGPLAIAMGAEGKGLRRLSRESCDTLVSIPMAGSVSSLNVSVATGVCLFEA
VRQRLA

Sequences:

>Translated_246_residues
MKKQDMTFGLHAVEAVLKNSPERVIEMWVLQGRDDARLTPILAMAAEWGVSVQYASRKALDEKSDGGQHQGVVAKVKPAK
ILSDNELSAMLEKTEVPFLLILDGVTDPHNLGACLRNADAAGVHGVIVPKDNSVGLTPVVSKVACGAAEVVPLYQVTNLA
RTMRSLQEKGIWIIGAAGEADCELYQASLTGPLAIAMGAEGKGLRRLSRESCDTLVSIPMAGSVSSLNVSVATGVCLFEA
VRQRLA
>Mature_246_residues
MKKQDMTFGLHAVEAVLKNSPERVIEMWVLQGRDDARLTPILAMAAEWGVSVQYASRKALDEKSDGGQHQGVVAKVKPAK
ILSDNELSAMLEKTEVPFLLILDGVTDPHNLGACLRNADAAGVHGVIVPKDNSVGLTPVVSKVACGAAEVVPLYQVTNLA
RTMRSLQEKGIWIIGAAGEADCELYQASLTGPLAIAMGAEGKGLRRLSRESCDTLVSIPMAGSVSSLNVSVATGVCLFEA
VRQRLA

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=220, Percent_Identity=32.7272727272727, Blast_Score=95, Evalue=7e-20,
Organism=Escherichia coli, GI1790623, Length=240, Percent_Identity=64.1666666666667, Blast_Score=330, Evalue=4e-92,
Organism=Escherichia coli, GI1788935, Length=249, Percent_Identity=28.9156626506024, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI1790083, Length=156, Percent_Identity=31.4102564102564, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17555908, Length=248, Percent_Identity=29.4354838709677, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324775, Length=285, Percent_Identity=27.3684210526316, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26089; Mature: 26089

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQDMTFGLHAVEAVLKNSPERVIEMWVLQGRDDARLTPILAMAAEWGVSVQYASRKAL
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEHHHHHC
DEKSDGGQHQGVVAKVKPAKILSDNELSAMLEKTEVPFLLILDGVTDPHNLGACLRNADA
CCCCCCCCCCCEEEEECCCCEECCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCC
AGVHGVIVPKDNSVGLTPVVSKVACGAAEVVPLYQVTNLARTMRSLQEKGIWIIGAAGEA
CCCEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCC
DCELYQASLTGPLAIAMGAEGKGLRRLSRESCDTLVSIPMAGSVSSLNVSVATGVCLFEA
CCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHHEEECCCCCCCCCCEEHHHHHHHHHHH
VRQRLA
HHHHCC
>Mature Secondary Structure
MKKQDMTFGLHAVEAVLKNSPERVIEMWVLQGRDDARLTPILAMAAEWGVSVQYASRKAL
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEHHHHHC
DEKSDGGQHQGVVAKVKPAKILSDNELSAMLEKTEVPFLLILDGVTDPHNLGACLRNADA
CCCCCCCCCCCEEEEECCCCEECCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCC
AGVHGVIVPKDNSVGLTPVVSKVACGAAEVVPLYQVTNLARTMRSLQEKGIWIIGAAGEA
CCCEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCC
DCELYQASLTGPLAIAMGAEGKGLRRLSRESCDTLVSIPMAGSVSSLNVSVATGVCLFEA
CCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHHEEECCCCCCCCCCEEHHHHHHHHHHH
VRQRLA
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]