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The map label for this gene is amiB [H]

Identifier: 167625543

GI number: 167625543

Start: 4434332

End: 4435654

Strand: Reverse

Name: amiB [H]

Synonym: Shal_3637

Alternate gene names: 167625543

Gene position: 4435654-4434332 (Counterclockwise)

Preceding gene: 167625544

Following gene: 167625542

Centisome position: 84.86

GC content: 42.18

Gene sequence:

>1323_bases
ATGATAATAAACAGTAATATATTTAGACTTTTTTTACTTCTTGTTAGTTTTGGTGCTTCATTTTTTGCGCAGAGTGCGAA
TAAACTCGACAGCGTAAGAATTTGGGCTGCGCCAGAGTCGACGCGTATCGTATTCGATTTGAGCCAGTCTCCAGATTACA
GCTATTTTACGCTGACAAATCCTTATCGCTTAGTCGTCGATTTAAAAAAGTCATCTAATAAAGTGGACTTAAATAAGATT
AAAAACAACAGCAAATTGGTCAAAAAGGTGCGTAAGAGTACGCCACCGGAAAAGGGCACTTTGCGTTTAGTTATCGATCT
CGTTAAGCCAGTAAAAGCCAATCTATTTGCACTGCCTGCAACAGCCCCATACGGTAATCGTTTGGTAGTTGATTTAGATA
GCGGTGGTTCAACAGCTAAAAAACCTCCGGTGAGTAAGCCAAAAAATCAATTACGAGACGTGGTTATTGCGATTGACGCT
GGTCATGGTGGTGAGGATCCCGGCTCTATTGGACCGACCGGCATATATGAGAAAAAGTTAGTATTAGAAATATCTAAAAA
AGTTGCTCAAAAGATTAATGCGACACCAGGCATGCGAGCGGTTATGACGCGTACTGGTGACTATTTCGTTAACTTAAATA
AGCGCTCTGAAATTGCTCGTAACAGTAAAGCTGACCTGCTGATTTCTATCCATGCAGACGCTTTTACCTCGCCTCAGCCT
CAAGGTGCCTCGGTATGGGTATTGTCTATGCGCCGTGCCAACAGTGAAATTGGTCGCTGGTTAGAGCAAAAAGAGAAGCA
TTCTGAACTGCTCGGTGGTGCTGGTGAGATTATACAAAATACTGATAATGAACAGTATCTTGCGATGACTTTATTAGATA
TGTCGATGGACCGCTCTATGGCGATTAGCCATAACATCGCTGGAGATGTGTTATCTAATCTAGGGCGGGTGACAAAACTA
CATAAGCATAAGCCTGAATCTGCGAGCTTTGCTGTACTCAAGTCGCCAGACATTCCGTCTATTTTGGTCGAAACAGGGTT
TATTTCTAACCCGAGGGAAGAGCGCCTATTATCGCAACGAGATCATCAAAATAATGTCGCCAAAGCCATCCATAAAGGCG
TGATGAAGTATTTTGAAGCCAATCCTCCTGTGAATTCAATGTTGGCAAGTAAGAGCAGTGTTGAGCACAAGGTTTGCAGT
GGTGAATCGTTATCCGTTATTGCCCATCGTTATCAGATCTCAATAAACAGTTTGAAGAAGGCAAATAACCTTAAATCAAA
TAGCATTCGAATCGGTCAGAGACTGATTATCCCCAGAGCTTAG

Upstream 100 bases:

>100_bases
GATATACATATCCATATTAAATATGTTAATACTGGACGACAAGTCGAGTTACAGGCGCTTTCTTCTGAAGGTGAAAAAAT
ATTAGCGGCCCTAAATCATA

Downstream 100 bases:

>100_bases
GCTGTTAAGAGTAAACCATGGCTATAGAAAAGTTACCGCCTCAATTAGCAAACCAGATCGCTGCGGGCGAAGTGGTTGAG
CGTCCTGCATCTGTCATCAA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MIINSNIFRLFLLLVSFGASFFAQSANKLDSVRIWAAPESTRIVFDLSQSPDYSYFTLTNPYRLVVDLKKSSNKVDLNKI
KNNSKLVKKVRKSTPPEKGTLRLVIDLVKPVKANLFALPATAPYGNRLVVDLDSGGSTAKKPPVSKPKNQLRDVVIAIDA
GHGGEDPGSIGPTGIYEKKLVLEISKKVAQKINATPGMRAVMTRTGDYFVNLNKRSEIARNSKADLLISIHADAFTSPQP
QGASVWVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNTDNEQYLAMTLLDMSMDRSMAISHNIAGDVLSNLGRVTKL
HKHKPESASFAVLKSPDIPSILVETGFISNPREERLLSQRDHQNNVAKAIHKGVMKYFEANPPVNSMLASKSSVEHKVCS
GESLSVIAHRYQISINSLKKANNLKSNSIRIGQRLIIPRA

Sequences:

>Translated_440_residues
MIINSNIFRLFLLLVSFGASFFAQSANKLDSVRIWAAPESTRIVFDLSQSPDYSYFTLTNPYRLVVDLKKSSNKVDLNKI
KNNSKLVKKVRKSTPPEKGTLRLVIDLVKPVKANLFALPATAPYGNRLVVDLDSGGSTAKKPPVSKPKNQLRDVVIAIDA
GHGGEDPGSIGPTGIYEKKLVLEISKKVAQKINATPGMRAVMTRTGDYFVNLNKRSEIARNSKADLLISIHADAFTSPQP
QGASVWVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNTDNEQYLAMTLLDMSMDRSMAISHNIAGDVLSNLGRVTKL
HKHKPESASFAVLKSPDIPSILVETGFISNPREERLLSQRDHQNNVAKAIHKGVMKYFEANPPVNSMLASKSSVEHKVCS
GESLSVIAHRYQISINSLKKANNLKSNSIRIGQRLIIPRA
>Mature_440_residues
MIINSNIFRLFLLLVSFGASFFAQSANKLDSVRIWAAPESTRIVFDLSQSPDYSYFTLTNPYRLVVDLKKSSNKVDLNKI
KNNSKLVKKVRKSTPPEKGTLRLVIDLVKPVKANLFALPATAPYGNRLVVDLDSGGSTAKKPPVSKPKNQLRDVVIAIDA
GHGGEDPGSIGPTGIYEKKLVLEISKKVAQKINATPGMRAVMTRTGDYFVNLNKRSEIARNSKADLLISIHADAFTSPQP
QGASVWVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNTDNEQYLAMTLLDMSMDRSMAISHNIAGDVLSNLGRVTKL
HKHKPESASFAVLKSPDIPSILVETGFISNPREERLLSQRDHQNNVAKAIHKGVMKYFEANPPVNSMLASKSSVEHKVCS
GESLSVIAHRYQISINSLKKANNLKSNSIRIGQRLIIPRA

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1790611, Length=426, Percent_Identity=37.3239436619718, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI87082163, Length=381, Percent_Identity=37.5328083989501, Blast_Score=232, Evalue=3e-62,
Organism=Escherichia coli, GI1788776, Length=225, Percent_Identity=42.2222222222222, Blast_Score=174, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 48484; Mature: 48484

Theoretical pI: Translated: 10.57; Mature: 10.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIINSNIFRLFLLLVSFGASFFAQSANKLDSVRIWAAPESTRIVFDLSQSPDYSYFTLTN
CEECCHHHHHHHHHHHHCHHHHHHHHCCCCCEEEEECCCCCEEEEEECCCCCCCEEEECC
PYRLVVDLKKSSNKVDLNKIKNNSKLVKKVRKSTPPEKGTLRLVIDLVKPVKANLFALPA
CEEEEEEECCCCCEEEHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEECC
TAPYGNRLVVDLDSGGSTAKKPPVSKPKNQLRDVVIAIDAGHGGEDPGSIGPTGIYEKKL
CCCCCCEEEEEECCCCCCCCCCCCCCCHHHHCEEEEEEECCCCCCCCCCCCCCCCHHHHH
VLEISKKVAQKINATPGMRAVMTRTGDYFVNLNKRSEIARNSKADLLISIHADAFTSPQP
HHHHHHHHHHHHCCCCCCCEEEECCCCEEEEECCHHHHHCCCCCCEEEEEECCCCCCCCC
QGASVWVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNTDNEQYLAMTLLDMSMDRSM
CCCEEEEEEEHHCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEEEHHHHCCCCH
AISHNIAGDVLSNLGRVTKLHKHKPESASFAVLKSPDIPSILVETGFISNPREERLLSQR
HHHHCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCCCCCHHHHHHHHH
DHQNNVAKAIHKGVMKYFEANPPVNSMLASKSSVEHKVCSGESLSVIAHRYQISINSLKK
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHCHHHHHHHHCCCCCCEEEEEEEEEEHHHHHH
ANNLKSNSIRIGQRLIIPRA
HCCCCCCCEEECCEEECCCC
>Mature Secondary Structure
MIINSNIFRLFLLLVSFGASFFAQSANKLDSVRIWAAPESTRIVFDLSQSPDYSYFTLTN
CEECCHHHHHHHHHHHHCHHHHHHHHCCCCCEEEEECCCCCEEEEEECCCCCCCEEEECC
PYRLVVDLKKSSNKVDLNKIKNNSKLVKKVRKSTPPEKGTLRLVIDLVKPVKANLFALPA
CEEEEEEECCCCCEEEHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEECC
TAPYGNRLVVDLDSGGSTAKKPPVSKPKNQLRDVVIAIDAGHGGEDPGSIGPTGIYEKKL
CCCCCCEEEEEECCCCCCCCCCCCCCCHHHHCEEEEEEECCCCCCCCCCCCCCCCHHHHH
VLEISKKVAQKINATPGMRAVMTRTGDYFVNLNKRSEIARNSKADLLISIHADAFTSPQP
HHHHHHHHHHHHCCCCCCCEEEECCCCEEEEECCHHHHHCCCCCCEEEEEECCCCCCCCC
QGASVWVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNTDNEQYLAMTLLDMSMDRSM
CCCEEEEEEEHHCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEEEHHHHCCCCH
AISHNIAGDVLSNLGRVTKLHKHKPESASFAVLKSPDIPSILVETGFISNPREERLLSQR
HHHHCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCCCCCHHHHHHHHH
DHQNNVAKAIHKGVMKYFEANPPVNSMLASKSSVEHKVCSGESLSVIAHRYQISINSLKK
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHCHHHHHHHHCCCCCCEEEEEEEEEEHHHHHH
ANNLKSNSIRIGQRLIIPRA
HCCCCCCCEEECCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7511774; 7610040; 9278503; 1594459 [H]