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The map label for this gene is miaA

Identifier: 167625541

GI number: 167625541

Start: 4431485

End: 4432411

Strand: Reverse

Name: miaA

Synonym: Shal_3635

Alternate gene names: 167625541

Gene position: 4432411-4431485 (Counterclockwise)

Preceding gene: 167625542

Following gene: 167625540

Centisome position: 84.8

GC content: 44.77

Gene sequence:

>927_bases
GTGACCGATAATAAATCACAAAAAGTTATTACCTTAATGGGCCCTACTGCCTCTGGTAAAACCGCATTAGCGATTGAGCT
TGTTAAGCAGTATAACTGTGAAATTATCTCTGTGGATTCAGCATTGATCTACAAAGAGATGGATATCGGCACCGCTAAGC
CAGATGCTGCCGAACAAGCAGCCGCTCCCCATCGACTTATTGACATTATTGATCCGGCAAAGAGTTATTCTGCGGCGGAC
TTTAGACGCGATGCATTGGCTCAGATTGAAGATATTTTGAGTCGTGGAAAGACGCCATTGCTGGTCGGCGGTACCATGAT
GTACTTCAAGGCTTTGCTAGAGGGGTTGTCACCGCTTCCGGCGGCAGATGAAGTGGTTAGAGCGCAGATAGCTTGCGAAG
CACAAGAGTTTGGCTGGCAAGCACTGCACGATCAATTAAGAGAAATAGATCCTGTTTCTGCTGAGCGAATTCATCCCAAT
GATCCACAGCGGCTCGCTAGAGCGCTGGAAGTCTATCGCATAAGTGGTCAGTCGTTAACTGAGTTAACTAAAACTAAATC
TGAAGCATTCCCTTACGAAGCGGTGCAATTTGCGATTGCACCAAGTGACAGAAAGGTGTTACATAGAGCTATAGAAACTC
GATTTAAAACCATGTTAACACTAGGGTTTACTGCTGAAGTTGAGCGTTTAAAGGCTCGAGGTGATTTGGATTTAGACCTG
CCCTCAATGCGCTGTGTAGGATATAGGCAGTGTTGGCAGTATTTAGATGGTGAAATTGACTATGAAACTATGGTTGAAAA
AGCTATTGTTGCTACTAGGCAATTAGCCAAACGTCAATTAACATGGTTACGCGGTTGGCCTGATTTAATATGGCTAGAAA
GCGGAGCTGAAGATAATCTTGCGACAGTAATGCGACATAGTCGCTAG

Upstream 100 bases:

>100_bases
GCGTGGTTTGGCCTAAATGCCTTGCCTGATGATGTGCAACGCGAGCTTTATAATTTATCCCAAGATTTACCTTGGGAACA
ATGGATGAAAGAAAATCAGA

Downstream 100 bases:

>100_bases
CTTATTAGCTGCCGCTGTATAATACTAAAACCAAAGATACAGTTTTATCGTATTAGAATAACTATTAATTTTAATTAAAA
AGGAAATTAGAAAATGGCAA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MTDNKSQKVITLMGPTASGKTALAIELVKQYNCEIISVDSALIYKEMDIGTAKPDAAEQAAAPHRLIDIIDPAKSYSAAD
FRRDALAQIEDILSRGKTPLLVGGTMMYFKALLEGLSPLPAADEVVRAQIACEAQEFGWQALHDQLREIDPVSAERIHPN
DPQRLARALEVYRISGQSLTELTKTKSEAFPYEAVQFAIAPSDRKVLHRAIETRFKTMLTLGFTAEVERLKARGDLDLDL
PSMRCVGYRQCWQYLDGEIDYETMVEKAIVATRQLAKRQLTWLRGWPDLIWLESGAEDNLATVMRHSR

Sequences:

>Translated_308_residues
MTDNKSQKVITLMGPTASGKTALAIELVKQYNCEIISVDSALIYKEMDIGTAKPDAAEQAAAPHRLIDIIDPAKSYSAAD
FRRDALAQIEDILSRGKTPLLVGGTMMYFKALLEGLSPLPAADEVVRAQIACEAQEFGWQALHDQLREIDPVSAERIHPN
DPQRLARALEVYRISGQSLTELTKTKSEAFPYEAVQFAIAPSDRKVLHRAIETRFKTMLTLGFTAEVERLKARGDLDLDL
PSMRCVGYRQCWQYLDGEIDYETMVEKAIVATRQLAKRQLTWLRGWPDLIWLESGAEDNLATVMRHSR
>Mature_307_residues
TDNKSQKVITLMGPTASGKTALAIELVKQYNCEIISVDSALIYKEMDIGTAKPDAAEQAAAPHRLIDIIDPAKSYSAADF
RRDALAQIEDILSRGKTPLLVGGTMMYFKALLEGLSPLPAADEVVRAQIACEAQEFGWQALHDQLREIDPVSAERIHPND
PQRLARALEVYRISGQSLTELTKTKSEAFPYEAVQFAIAPSDRKVLHRAIETRFKTMLTLGFTAEVERLKARGDLDLDLP
SMRCVGYRQCWQYLDGEIDYETMVEKAIVATRQLAKRQLTWLRGWPDLIWLESGAEDNLATVMRHSR

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=309, Percent_Identity=28.4789644012945, Blast_Score=125, Evalue=4e-29,
Organism=Escherichia coli, GI1790613, Length=287, Percent_Identity=68.2926829268293, Blast_Score=404, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI25144712, Length=244, Percent_Identity=30.7377049180328, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324848, Length=293, Percent_Identity=28.3276450511945, Blast_Score=133, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24649830, Length=238, Percent_Identity=31.5126050420168, Blast_Score=122, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_SHEHH (B0TU65)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001675835.1
- ProteinModelPortal:   B0TU65
- SMR:   B0TU65
- GeneID:   5904874
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_3635
- HOGENOM:   HBG559459
- OMA:   LPYTVAN
- ProtClustDB:   PRK00091
- BioCyc:   SHAL458817:SHAL_3635-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34522; Mature: 34391

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDNKSQKVITLMGPTASGKTALAIELVKQYNCEIISVDSALIYKEMDIGTAKPDAAEQA
CCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEECHHHHEEECCCCCCCCCHHHHH
AAPHRLIDIIDPAKSYSAADFRRDALAQIEDILSRGKTPLLVGGTMMYFKALLEGLSPLP
CCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCCCC
AADEVVRAQIACEAQEFGWQALHDQLREIDPVSAERIHPNDPQRLARALEVYRISGQSLT
CHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHH
ELTKTKSEAFPYEAVQFAIAPSDRKVLHRAIETRFKTMLTLGFTAEVERLKARGDLDLDL
HHHHHHHHCCCHHHEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCC
PSMRCVGYRQCWQYLDGEIDYETMVEKAIVATRQLAKRQLTWLRGWPDLIWLESGAEDNL
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHH
ATVMRHSR
HHHHHCCC
>Mature Secondary Structure 
TDNKSQKVITLMGPTASGKTALAIELVKQYNCEIISVDSALIYKEMDIGTAKPDAAEQA
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEECHHHHEEECCCCCCCCCHHHHH
AAPHRLIDIIDPAKSYSAADFRRDALAQIEDILSRGKTPLLVGGTMMYFKALLEGLSPLP
CCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCCCC
AADEVVRAQIACEAQEFGWQALHDQLREIDPVSAERIHPNDPQRLARALEVYRISGQSLT
CHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHH
ELTKTKSEAFPYEAVQFAIAPSDRKVLHRAIETRFKTMLTLGFTAEVERLKARGDLDLDL
HHHHHHHHCCCHHHEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCC
PSMRCVGYRQCWQYLDGEIDYETMVEKAIVATRQLAKRQLTWLRGWPDLIWLESGAEDNL
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHH
ATVMRHSR
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA