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The map label for this gene is cysI

Identifier: 167625450

GI number: 167625450

Start: 4319528

End: 4321225

Strand: Reverse

Name: cysI

Synonym: Shal_3542

Alternate gene names: 167625450

Gene position: 4321225-4319528 (Counterclockwise)

Preceding gene: 167625451

Following gene: 167625449

Centisome position: 82.67

GC content: 48.82

Gene sequence:

>1698_bases
ATGAGTGAGCAAAAGTTAGCAGTAAATGAATACCTAAAAACCGACAGTGATTACCTGCGCGGTACGATCCAACAGGGTTT
GAATACCGCAGTCACCGGTGCATTTAGTGAAGGCGATCAACAGTTGATCAAGTTCCATGGTTTTTACCAGCAAGACGATC
GCGACCTGCGTAACGAGCGAAAAGAGCAAAAACTAGAGCCTCTATATAGCTTTATGTTGCGTGCTCGTGTGGCGGGTGGC
GTATGTTCGCCAGAGCAGTGGTTAGCGGTCGATAAAATTGCCTCGAACCTGACAAGCGCAAACAGTATCCGTTTAACGAC
GCGTCAAACGTTTCAGTATCATGGTATTCCCAAGCGTAACCTGAAGACGATCATTCAAGATCTCGACAGAGAAGCGTTGG
ACTCTATCGCTGCATGTGGTGATGTAAACCGCAACGTGATGTGTAACCCAAACCCCGTTGAGTCTAAGCTGCATCAACAA
GCTTATGCTTATGCAAAACAGCTATCGGATAACATGTTGCCACATACCAAGGCTTACGCCGAGATCTGGCTCGATAACGA
GAAGCTTGTGACCACTGAGGGGGAAGAGGTGGAGCCGGTTTACGGTCAAACCTATCTGCCACGTAAATTTAAGATGGCGG
TTGCCGTGCCGCCAGATAATGATGTGGATGTTTACACTAACGATCTGGGCTTTATTGCCGTTGCCGAAGGTGATGAGTTA
GTAGGCTTTAATATGGTAGCGGGTGGGGGCATGGGCTCGACCCATGGTGAAGTCTCGACCTTCCCACGCTTGGCTGATGA
CTTTGGTTATATTAAGGCCGAAGATTCCTTAAAGTTTGCCGAAGCGGTGATGACGATTCAGCGTGACTGGGGTAATCGTG
AAAACCGTAAGTTATCACGCCTAAAGTACACCATAGTGAAGCATGGGTTTGAGACCTTTAAGGCTGAAATCGAGGCGCGC
ACAGGCATTAAGTTTGAGCCTAGACGTGATGTGGTGATTGGCGATCGCGGTGACCGTTATGGCTGGAAGCAGGGCGTCGA
TGATAATTGGCACTTAACCCTGTTTATCGAAGGCGGCCGTGTTAAAGACTTACCGGGTCAGCCGCTGCAAACAGGCCTAA
GAGAGATAGCAAAAATTCACAGAGGTGATTTTCGCATGACCTCAAACCAGAATATGATAATTGCTGGGGTAGCGAGTGCA
GACAAGGAGCAGATTGAAGGCTTAGCAAGACAGTATGGCTTACTCGGCAAGCTTATCACCGAAACACGTGGCCACTCTAT
TGCCTGTGTGGCATTGCCAACTTGCGCACTGGCGATGGCCGAAGCCGAGCGTTATTTCCCCGACTTTTTGACTAAGGTTG
AGGCGCTGCAACAAAAGCATGGTTTCCTCGACCAAGGCATCGTTATTCGTATGACGGGTTGCCCTAATGGCTGCGCTAGA
CCCTTTGCGGCTGAAATTGGTTTAGTGGGTAAAGCGCCTGGTCGTTACAACCTCTATTTGGGCGCCAGTTTTGAAGGTAC
ACGTCTGAACAAATTATATCGTGAAAATATCCAAGAAGCGGAGATCCTTGACCAATTAGATACCCTGTTTGCACAATATG
CTTCACAGCGCCAAGCGGGTGAAACGTTTGGTAACTACACCGTGCGTAGCGGCGTTGTTGCGGCTGTAAATGATGCGGCT
AAGGACTTTCATGGTTGA

Upstream 100 bases:

>100_bases
AAAAAGATGTCTACTGATTTAAACAAGCAAAGCAATGATCAGCGACGCTAGGGTTTAACTGGCGCGAGAGCAGAAAAGAA
TTTGAAGAGAGTACCTTGCC

Downstream 100 bases:

>100_bases
TACTCATAATATTGCTGATCCGGTGATTACATCAACTGCGCTCAAGGCGTTATTGAGCGCGCCTAAAGTAGAGCAGCAAG
CAGAATTAAAGCGAGTCAAT

Product: sulfite reductase subunit beta

Products: NA

Alternate protein names: SiR-HP; SiRHP

Number of amino acids: Translated: 565; Mature: 564

Protein sequence:

>565_residues
MSEQKLAVNEYLKTDSDYLRGTIQQGLNTAVTGAFSEGDQQLIKFHGFYQQDDRDLRNERKEQKLEPLYSFMLRARVAGG
VCSPEQWLAVDKIASNLTSANSIRLTTRQTFQYHGIPKRNLKTIIQDLDREALDSIAACGDVNRNVMCNPNPVESKLHQQ
AYAYAKQLSDNMLPHTKAYAEIWLDNEKLVTTEGEEVEPVYGQTYLPRKFKMAVAVPPDNDVDVYTNDLGFIAVAEGDEL
VGFNMVAGGGMGSTHGEVSTFPRLADDFGYIKAEDSLKFAEAVMTIQRDWGNRENRKLSRLKYTIVKHGFETFKAEIEAR
TGIKFEPRRDVVIGDRGDRYGWKQGVDDNWHLTLFIEGGRVKDLPGQPLQTGLREIAKIHRGDFRMTSNQNMIIAGVASA
DKEQIEGLARQYGLLGKLITETRGHSIACVALPTCALAMAEAERYFPDFLTKVEALQQKHGFLDQGIVIRMTGCPNGCAR
PFAAEIGLVGKAPGRYNLYLGASFEGTRLNKLYRENIQEAEILDQLDTLFAQYASQRQAGETFGNYTVRSGVVAAVNDAA
KDFHG

Sequences:

>Translated_565_residues
MSEQKLAVNEYLKTDSDYLRGTIQQGLNTAVTGAFSEGDQQLIKFHGFYQQDDRDLRNERKEQKLEPLYSFMLRARVAGG
VCSPEQWLAVDKIASNLTSANSIRLTTRQTFQYHGIPKRNLKTIIQDLDREALDSIAACGDVNRNVMCNPNPVESKLHQQ
AYAYAKQLSDNMLPHTKAYAEIWLDNEKLVTTEGEEVEPVYGQTYLPRKFKMAVAVPPDNDVDVYTNDLGFIAVAEGDEL
VGFNMVAGGGMGSTHGEVSTFPRLADDFGYIKAEDSLKFAEAVMTIQRDWGNRENRKLSRLKYTIVKHGFETFKAEIEAR
TGIKFEPRRDVVIGDRGDRYGWKQGVDDNWHLTLFIEGGRVKDLPGQPLQTGLREIAKIHRGDFRMTSNQNMIIAGVASA
DKEQIEGLARQYGLLGKLITETRGHSIACVALPTCALAMAEAERYFPDFLTKVEALQQKHGFLDQGIVIRMTGCPNGCAR
PFAAEIGLVGKAPGRYNLYLGASFEGTRLNKLYRENIQEAEILDQLDTLFAQYASQRQAGETFGNYTVRSGVVAAVNDAA
KDFHG
>Mature_564_residues
SEQKLAVNEYLKTDSDYLRGTIQQGLNTAVTGAFSEGDQQLIKFHGFYQQDDRDLRNERKEQKLEPLYSFMLRARVAGGV
CSPEQWLAVDKIASNLTSANSIRLTTRQTFQYHGIPKRNLKTIIQDLDREALDSIAACGDVNRNVMCNPNPVESKLHQQA
YAYAKQLSDNMLPHTKAYAEIWLDNEKLVTTEGEEVEPVYGQTYLPRKFKMAVAVPPDNDVDVYTNDLGFIAVAEGDELV
GFNMVAGGGMGSTHGEVSTFPRLADDFGYIKAEDSLKFAEAVMTIQRDWGNRENRKLSRLKYTIVKHGFETFKAEIEART
GIKFEPRRDVVIGDRGDRYGWKQGVDDNWHLTLFIEGGRVKDLPGQPLQTGLREIAKIHRGDFRMTSNQNMIIAGVASAD
KEQIEGLARQYGLLGKLITETRGHSIACVALPTCALAMAEAERYFPDFLTKVEALQQKHGFLDQGIVIRMTGCPNGCARP
FAAEIGLVGKAPGRYNLYLGASFEGTRLNKLYRENIQEAEILDQLDTLFAQYASQRQAGETFGNYTVRSGVVAAVNDAAK
DFHG

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family

Homologues:

Organism=Escherichia coli, GI1789122, Length=561, Percent_Identity=55.4367201426025, Blast_Score=667, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322597, Length=594, Percent_Identity=42.5925925925926, Blast_Score=510, Evalue=1e-145,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): CYSI_SHEHH (B0TTE4)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001675744.1
- ProteinModelPortal:   B0TTE4
- SMR:   B0TTE4
- GeneID:   5905741
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_3542
- HOGENOM:   HBG626671
- OMA:   TRQAFQM
- ProtClustDB:   PRK13504
- BioCyc:   SHAL458817:SHAL_3542-MONOMER
- HAMAP:   MF_01540
- InterPro:   IPR011786
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066
- PRINTS:   PR00397
- TIGRFAMs:   TIGR02041

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; SSF55124 NiR_SiRalpha_1/3

EC number: =1.8.1.2

Molecular weight: Translated: 63082; Mature: 62951

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQKLAVNEYLKTDSDYLRGTIQQGLNTAVTGAFSEGDQQLIKFHGFYQQDDRDLRNER
CCCCHHHHHHHHHCCHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHH
KEQKLEPLYSFMLRARVAGGVCSPEQWLAVDKIASNLTSANSIRLTTRQTFQYHGIPKRN
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEHHHEECCCCHHH
LKTIIQDLDREALDSIAACGDVNRNVMCNPNPVESKLHQQAYAYAKQLSDNMLPHTKAYA
HHHHHHHHCHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
EIWLDNEKLVTTEGEEVEPVYGQTYLPRKFKMAVAVPPDNDVDVYTNDLGFIAVAEGDEL
HEEECCCEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEECCCCEEEEECCCCE
VGFNMVAGGGMGSTHGEVSTFPRLADDFGYIKAEDSLKFAEAVMTIQRDWGNRENRKLSR
EEEEEEECCCCCCCCCCHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHCCCCCHHHHHH
LKYTIVKHGFETFKAEIEARTGIKFEPRRDVVIGDRGDRYGWKQGVDDNWHLTLFIEGGR
HHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEECCCCCCCCCCCCCCCCEEEEEEEECCE
VKDLPGQPLQTGLREIAKIHRGDFRMTSNQNMIIAGVASADKEQIEGLARQYGLLGKLIT
ECCCCCCHHHHHHHHHHHHHCCCEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
ETRGHSIACVALPTCALAMAEAERYFPDFLTKVEALQQKHGFLDQGIVIRMTGCPNGCAR
HCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCC
PFAAEIGLVGKAPGRYNLYLGASFEGTRLNKLYRENIQEAEILDQLDTLFAQYASQRQAG
CHHHHCCCEECCCCCEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
ETFGNYTVRSGVVAAVNDAAKDFHG
HHHCCEEHHCCCEEEHHHHHHHCCC
>Mature Secondary Structure 
SEQKLAVNEYLKTDSDYLRGTIQQGLNTAVTGAFSEGDQQLIKFHGFYQQDDRDLRNER
CCCHHHHHHHHHCCHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHH
KEQKLEPLYSFMLRARVAGGVCSPEQWLAVDKIASNLTSANSIRLTTRQTFQYHGIPKRN
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEHHHEECCCCHHH
LKTIIQDLDREALDSIAACGDVNRNVMCNPNPVESKLHQQAYAYAKQLSDNMLPHTKAYA
HHHHHHHHCHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
EIWLDNEKLVTTEGEEVEPVYGQTYLPRKFKMAVAVPPDNDVDVYTNDLGFIAVAEGDEL
HEEECCCEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEECCCCEEEEECCCCE
VGFNMVAGGGMGSTHGEVSTFPRLADDFGYIKAEDSLKFAEAVMTIQRDWGNRENRKLSR
EEEEEEECCCCCCCCCCHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHCCCCCHHHHHH
LKYTIVKHGFETFKAEIEARTGIKFEPRRDVVIGDRGDRYGWKQGVDDNWHLTLFIEGGR
HHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEECCCCCCCCCCCCCCCCEEEEEEEECCE
VKDLPGQPLQTGLREIAKIHRGDFRMTSNQNMIIAGVASADKEQIEGLARQYGLLGKLIT
ECCCCCCHHHHHHHHHHHHHCCCEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
ETRGHSIACVALPTCALAMAEAERYFPDFLTKVEALQQKHGFLDQGIVIRMTGCPNGCAR
HCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCC
PFAAEIGLVGKAPGRYNLYLGASFEGTRLNKLYRENIQEAEILDQLDTLFAQYASQRQAG
CHHHHCCCEECCCCCEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
ETFGNYTVRSGVVAAVNDAAKDFHG
HHHCCEEHHCCCEEEHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA