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The map label for this gene is cysJ [H]

Identifier: 167625451

GI number: 167625451

Start: 4321309

End: 4323117

Strand: Reverse

Name: cysJ [H]

Synonym: Shal_3543

Alternate gene names: 167625451

Gene position: 4323117-4321309 (Counterclockwise)

Preceding gene: 167625456

Following gene: 167625450

Centisome position: 82.71

GC content: 49.31

Gene sequence:

>1809_bases
ATGTTGTTAAAAGAGCTTTCATCACTCGCTTCGCCGCTATCTGCTGGCCAAGTGGATAAGTTAAAGCAGCTCACTGCAGA
GCTGAGTGCCGTGCAACTTGCTTGGGTGAGTGGCTATCTTGCTGCAGCATCTGAGCAAGGCGTGGCGGGTGCGCCACAAG
TCGGGGGGAGTTCTGAAGCTAGTTCGCAACCGGGTAGCGAGCAAACTATCAGCATACTTTACGGCAGCCAAACGGGTAAT
GGTCGTGGCATTGCTTCTGAGCTTGCCGAAAAAGCACAGGCACAAGGCTATGCGGTCAACTTAGTGTCTATGGGGGAGTA
TAAGACACGTCAGCTTAAGCAAGAGAGCTTATTGCTATTAGTGGTCAGTACCCATGGTGAAGGTGAAGCCCCTGATGATG
CCATCGAGCTGCATAAATTTTTAAGCTCTAAGCGCGCCCCTAAACTGGATAACCTAAATTACTCTGTGTTGGCACTGGGG
GATTCGAGTTATGAGTTTTTCTGTCAAACGGGTAAAGATTTCGAGTCTCGTCTTAACGCATTAGGCGCGACGGCCATTGC
GCCATTGGTTGAGTGTGATGTTGATTACGAAGAGCAATCACTGCAGTGGCAAGCGGATGTCCTAGAAGCGGTTAAACCGT
TAGTACAGAGCAGCAGCGCAAGCGTAGTTTCAATCGCCAGCACTCAGGCTGTGACTTCAAGTTTTAGCAAACAAAAGCCT
TACACCGCAGAGCTACTCGTGAGTCAAAAGCTGACGGGGAGAGATTCTGATAGAGATATTCGCCATGTTGAAATTGAGCT
GGGTGAATCGGGTATTCAATATCAAGCGGGTGACGCCTTAGGTGTGTGGTTTACTAACTCACAAGCTCTAGTGACTGAGC
TGCTTGAAACATTAGCGCTTAATGGTGATGCGCAAGTGACTATCGGTAAAGAAACATTAACTTTGCGTGATGCACTGACA
AATAACAAAGAGCTGACGCAACTTTATCCGGGTTTAGTGACTGGATGGGCAGAGCTAAGCAAAAGCGGTGAGTTACAAGC
AATCAGCGCCGATAAAGCCTTAACCCGTGAGTATGTGAATAACAACCAAGTGGCTGACTTAGTGCGTCGATACCCAGCGT
CTGTGACCGCCGAGCAGTTTGTCGGCCTACTGCGCAGTATTACTCCAAGACTTTACTCTATCGCCTCGAGTCAGGCTGAA
GTGGAGTCTGAAGTGCACTTGACCGTGGCACTGGTTGAAGATGAACGTGACGGCGTCACTCGCTTTGGTGGGGCATCTCA
ATTCCTCGCAAGTGCCGAAGAGGGGCAAGAAGTGCAGGTGTACGTAGAGCCTAATCATCACTTCCGTTTACCTGAAAACC
CAGACACGTCAGTGATCATGGTCGGCCCCGGTACCGGTGTGGCCCCTTTTAGAGCCTTTATGCAAGAACGTGCAGCGCAA
GGGGTAGAAGGTAAGACTTGGTTGTTCTTTGGTAATCCCCATTTCGAGCAAGATTTCCTCTATCAAACTGAGTGGCAGCA
ATACCTGCAATCTGGTGAGTTATCTCGAATCGATTTGGCCTTTTCTCGCGACCAACAGCATAAGATTTATGTGCAGCACA
AGATCGTCGAGCAGGGTGAGGCGTTGTGGCAGTGGTTAGAGTCTGGCGCACATTTTTATCTCTGTGGTGACGCCGAGCGT
ATGGCCAAAGATGTGCACCAAGCGCTGCTAGATGTGGTCGTTAAATACGGTAATAAAACTGAACAGCAAGCGGCCGAGTA
CCTAGAAGAGTTAAGAGCGGCTAAGCGTTACCAAAAAGATGTCTACTGA

Upstream 100 bases:

>100_bases
GTATATTCCAAAATCGAATTAAAAATTATCTTTTATTCTTTTTTTCTTTTTCCAGGAGGGGATAAGCTTGCTGACATTAC
ACTGATACAGGGCCGTTTCC

Downstream 100 bases:

>100_bases
TTTAAACAAGCAAAGCAATGATCAGCGACGCTAGGGTTTAACTGGCGCGAGAGCAGAAAAGAATTTGAAGAGAGTACCTT
GCCATGAGTGAGCAAAAGTT

Product: sulfite reductase (NADPH) flavoprotein subunit alpha

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 602; Mature: 602

Protein sequence:

>602_residues
MLLKELSSLASPLSAGQVDKLKQLTAELSAVQLAWVSGYLAAASEQGVAGAPQVGGSSEASSQPGSEQTISILYGSQTGN
GRGIASELAEKAQAQGYAVNLVSMGEYKTRQLKQESLLLLVVSTHGEGEAPDDAIELHKFLSSKRAPKLDNLNYSVLALG
DSSYEFFCQTGKDFESRLNALGATAIAPLVECDVDYEEQSLQWQADVLEAVKPLVQSSSASVVSIASTQAVTSSFSKQKP
YTAELLVSQKLTGRDSDRDIRHVEIELGESGIQYQAGDALGVWFTNSQALVTELLETLALNGDAQVTIGKETLTLRDALT
NNKELTQLYPGLVTGWAELSKSGELQAISADKALTREYVNNNQVADLVRRYPASVTAEQFVGLLRSITPRLYSIASSQAE
VESEVHLTVALVEDERDGVTRFGGASQFLASAEEGQEVQVYVEPNHHFRLPENPDTSVIMVGPGTGVAPFRAFMQERAAQ
GVEGKTWLFFGNPHFEQDFLYQTEWQQYLQSGELSRIDLAFSRDQQHKIYVQHKIVEQGEALWQWLESGAHFYLCGDAER
MAKDVHQALLDVVVKYGNKTEQQAAEYLEELRAAKRYQKDVY

Sequences:

>Translated_602_residues
MLLKELSSLASPLSAGQVDKLKQLTAELSAVQLAWVSGYLAAASEQGVAGAPQVGGSSEASSQPGSEQTISILYGSQTGN
GRGIASELAEKAQAQGYAVNLVSMGEYKTRQLKQESLLLLVVSTHGEGEAPDDAIELHKFLSSKRAPKLDNLNYSVLALG
DSSYEFFCQTGKDFESRLNALGATAIAPLVECDVDYEEQSLQWQADVLEAVKPLVQSSSASVVSIASTQAVTSSFSKQKP
YTAELLVSQKLTGRDSDRDIRHVEIELGESGIQYQAGDALGVWFTNSQALVTELLETLALNGDAQVTIGKETLTLRDALT
NNKELTQLYPGLVTGWAELSKSGELQAISADKALTREYVNNNQVADLVRRYPASVTAEQFVGLLRSITPRLYSIASSQAE
VESEVHLTVALVEDERDGVTRFGGASQFLASAEEGQEVQVYVEPNHHFRLPENPDTSVIMVGPGTGVAPFRAFMQERAAQ
GVEGKTWLFFGNPHFEQDFLYQTEWQQYLQSGELSRIDLAFSRDQQHKIYVQHKIVEQGEALWQWLESGAHFYLCGDAER
MAKDVHQALLDVVVKYGNKTEQQAAEYLEELRAAKRYQKDVY
>Mature_602_residues
MLLKELSSLASPLSAGQVDKLKQLTAELSAVQLAWVSGYLAAASEQGVAGAPQVGGSSEASSQPGSEQTISILYGSQTGN
GRGIASELAEKAQAQGYAVNLVSMGEYKTRQLKQESLLLLVVSTHGEGEAPDDAIELHKFLSSKRAPKLDNLNYSVLALG
DSSYEFFCQTGKDFESRLNALGATAIAPLVECDVDYEEQSLQWQADVLEAVKPLVQSSSASVVSIASTQAVTSSFSKQKP
YTAELLVSQKLTGRDSDRDIRHVEIELGESGIQYQAGDALGVWFTNSQALVTELLETLALNGDAQVTIGKETLTLRDALT
NNKELTQLYPGLVTGWAELSKSGELQAISADKALTREYVNNNQVADLVRRYPASVTAEQFVGLLRSITPRLYSIASSQAE
VESEVHLTVALVEDERDGVTRFGGASQFLASAEEGQEVQVYVEPNHHFRLPENPDTSVIMVGPGTGVAPFRAFMQERAAQ
GVEGKTWLFFGNPHFEQDFLYQTEWQQYLQSGELSRIDLAFSRDQQHKIYVQHKIVEQGEALWQWLESGAHFYLCGDAER
MAKDVHQALLDVVVKYGNKTEQQAAEYLEELRAAKRYQKDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI7657393, Length=594, Percent_Identity=30.4713804713805, Blast_Score=247, Evalue=2e-65,
Organism=Homo sapiens, GI221316709, Length=593, Percent_Identity=30.185497470489, Blast_Score=243, Evalue=5e-64,
Organism=Homo sapiens, GI221316705, Length=603, Percent_Identity=30.016583747927, Blast_Score=239, Evalue=4e-63,
Organism=Homo sapiens, GI127139033, Length=603, Percent_Identity=29.6849087893864, Blast_Score=236, Evalue=6e-62,
Organism=Homo sapiens, GI221316707, Length=520, Percent_Identity=31.3461538461538, Blast_Score=218, Evalue=1e-56,
Organism=Homo sapiens, GI24041029, Length=599, Percent_Identity=28.881469115192, Blast_Score=200, Evalue=4e-51,
Organism=Homo sapiens, GI10835173, Length=642, Percent_Identity=26.9470404984424, Blast_Score=174, Evalue=3e-43,
Organism=Homo sapiens, GI40254422, Length=651, Percent_Identity=26.5745007680492, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI169790956, Length=422, Percent_Identity=28.1990521327014, Blast_Score=146, Evalue=7e-35,
Organism=Homo sapiens, GI169790958, Length=422, Percent_Identity=28.1990521327014, Blast_Score=145, Evalue=8e-35,
Organism=Escherichia coli, GI1789123, Length=594, Percent_Identity=51.1784511784512, Blast_Score=602, Evalue=1e-173,
Organism=Escherichia coli, GI1789154, Length=124, Percent_Identity=32.258064516129, Blast_Score=64, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17554134, Length=599, Percent_Identity=27.8797996661102, Blast_Score=226, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17566446, Length=574, Percent_Identity=27.8745644599303, Blast_Score=164, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17531441, Length=454, Percent_Identity=23.7885462555066, Blast_Score=91, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI32563863, Length=125, Percent_Identity=35.2, Blast_Score=69, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6321832, Length=637, Percent_Identity=28.7284144427002, Blast_Score=204, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6321143, Length=391, Percent_Identity=32.7365728900256, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6325305, Length=630, Percent_Identity=22.2222222222222, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6322597, Length=153, Percent_Identity=29.4117647058824, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17137192, Length=612, Percent_Identity=29.2483660130719, Blast_Score=235, Evalue=9e-62,
Organism=Drosophila melanogaster, GI24582192, Length=521, Percent_Identity=28.5988483685221, Blast_Score=196, Evalue=3e-50,
Organism=Drosophila melanogaster, GI24660907, Length=592, Percent_Identity=26.6891891891892, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24660903, Length=592, Percent_Identity=26.6891891891892, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI78706872, Length=521, Percent_Identity=28.2149712092131, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24583543, Length=521, Percent_Identity=28.2149712092131, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI78706876, Length=521, Percent_Identity=28.2149712092131, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24647438, Length=409, Percent_Identity=26.6503667481663, Blast_Score=88, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 65937; Mature: 65937

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLKELSSLASPLSAGQVDKLKQLTAELSAVQLAWVSGYLAAASEQGVAGAPQVGGSSEA
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
SSQPGSEQTISILYGSQTGNGRGIASELAEKAQAQGYAVNLVSMGEYKTRQLKQESLLLL
CCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEECCCHHHHHHCCCCEEEE
VVSTHGEGEAPDDAIELHKFLSSKRAPKLDNLNYSVLALGDSSYEFFCQTGKDFESRLNA
EEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEECCCCCHHHHHHH
LGATAIAPLVECDVDYEEQSLQWQADVLEAVKPLVQSSSASVVSIASTQAVTSSFSKQKP
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHCCC
YTAELLVSQKLTGRDSDRDIRHVEIELGESGIQYQAGDALGVWFTNSQALVTELLETLAL
CHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCEEECCCEEEEEEECCHHHHHHHHHHHHC
NGDAQVTIGKETLTLRDALTNNKELTQLYPGLVTGWAELSKSGELQAISADKALTREYVN
CCCCEEEECCHHEEHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHC
NNQVADLVRRYPASVTAEQFVGLLRSITPRLYSIASSQAEVESEVHLTVALVEDERDGVT
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEEEEEECCCCCCH
RFGGASQFLASAEEGQEVQVYVEPNHHFRLPENPDTSVIMVGPGTGVAPFRAFMQERAAQ
HCCCHHHHHHHCCCCCEEEEEECCCCCEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHC
GVEGKTWLFFGNPHFEQDFLYQTEWQQYLQSGELSRIDLAFSRDQQHKIYVQHKIVEQGE
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEHHHHHHHHH
ALWQWLESGAHFYLCGDAERMAKDVHQALLDVVVKYGNKTEQQAAEYLEELRAAKRYQKD
HHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
VY
CC
>Mature Secondary Structure
MLLKELSSLASPLSAGQVDKLKQLTAELSAVQLAWVSGYLAAASEQGVAGAPQVGGSSEA
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
SSQPGSEQTISILYGSQTGNGRGIASELAEKAQAQGYAVNLVSMGEYKTRQLKQESLLLL
CCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEECCCHHHHHHCCCCEEEE
VVSTHGEGEAPDDAIELHKFLSSKRAPKLDNLNYSVLALGDSSYEFFCQTGKDFESRLNA
EEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEECCCCCHHHHHHH
LGATAIAPLVECDVDYEEQSLQWQADVLEAVKPLVQSSSASVVSIASTQAVTSSFSKQKP
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHCCC
YTAELLVSQKLTGRDSDRDIRHVEIELGESGIQYQAGDALGVWFTNSQALVTELLETLAL
CHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCEEECCCEEEEEEECCHHHHHHHHHHHHC
NGDAQVTIGKETLTLRDALTNNKELTQLYPGLVTGWAELSKSGELQAISADKALTREYVN
CCCCEEEECCHHEEHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHC
NNQVADLVRRYPASVTAEQFVGLLRSITPRLYSIASSQAEVESEVHLTVALVEDERDGVT
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEEEEEECCCCCCH
RFGGASQFLASAEEGQEVQVYVEPNHHFRLPENPDTSVIMVGPGTGVAPFRAFMQERAAQ
HCCCHHHHHHHCCCCCEEEEEECCCCCEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHC
GVEGKTWLFFGNPHFEQDFLYQTEWQQYLQSGELSRIDLAFSRDQQHKIYVQHKIVEQGE
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEHHHHHHHHH
ALWQWLESGAHFYLCGDAERMAKDVHQALLDVVVKYGNKTEQQAAEYLEELRAAKRYQKD
HHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
VY
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]