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The map label for this gene is cobQ [H]

Identifier: 167625365

GI number: 167625365

Start: 4208382

End: 4209983

Strand: Reverse

Name: cobQ [H]

Synonym: Shal_3454

Alternate gene names: 167625365

Gene position: 4209983-4208382 (Counterclockwise)

Preceding gene: 167625366

Following gene: 167625364

Centisome position: 80.54

GC content: 49.0

Gene sequence:

>1602_bases
GTGACAGTAAAAGTGGCAGTAACAGATAACAATAACGCCAATAATCTAACTAAAGTCTTAATGGTCCAAGGCACTACATC
GGATGCAGGTAAAAGCACTTTAGTGGCAGGCTTGTGTCGGCTGTTTGTCAGGCAAGGTGTAAAAGTCGCGCCGTTTAAGC
CGCAAAATATGGCGCTAAATAGTGCCGTGACGATTGATGGTGGTGAGATAGGCCGAGCTCAGGCACTGCAAGCTGTGGCC
TGTCATCTTGAGCCACAAATTGATTTTAATCCTATTTTATTAAAACCCAGTTCAGATACTGGCTCACAAGTGATTGTCCA
CGGTAAAGCCTTGATGAAGATGGAAGCCGAAGACTATTTTGGCAAAAATGCTGAGGGCTATCGGGTTAAGGCCATGGCTG
CAGTGCGTGAGTCATACCTGCGTTTAACTTCTGAGTACGACATGCTGCTGGTGGAAGGGGCTGGGAGTCCGGCTGAGATT
AATCTGCGAGAAGGTGATATCGCCAATATGGGTTTTGCCGAAGAGGTCGATTGCCCGGTGATTATTATTGCCGATATCGA
TAAGGGCGGCGTATTTGCCCACTTGGTGGGTACCTTGGCTCTTTTGAGTGAGTCGGAACAGGCGCGAGTAAAAGGCTTTG
TGATTAACCGTTTTCGTGGCGATGTGAGCCTGTTGCAAGGCGGGTTAGATTGGCTAGAAAGCTATACAAACAAGCCTGTG
CTAGGAGTATTGCCTTACCTGCACGACCTGCATTTAGATGCTGAAGATGCATTAATTGCCGCGCCAGAAAAGTCCAACGA
CACCAAGCTTAAGGTGTTAGTACTAGTACTGCCGCGGATCAGTAATCATACCGACTTTGATCCCTTAAGACTCAATCCAC
AGATAGACTTCAATTATGTGTCGTTGCAGACCCAAGCTCAAAGCCAGTCTCAAAATCAGTCTCAAAGCAAGCAAGCCCTG
CCGAGCGCCGATCTAATTATTATTCCTGGTAGCAAGAACGTGCGTGCCGATCTTGCGTTTATCCGTGAGCAGGGCTGGGA
CTGTGCGATTGACAAACACCTACGTTACGGTGGCAAGGTGCTTGGGATCTGCGGTGGTTATCAGATCTTAGGTCAGTCGA
TCGACGATCCAGATGGGATCGAAGATTACGCGGGCAGCAGTGAAGCCTTGGGTTTACTGCCATTAGTGACCGAGCTTACT
AGCACAAAGGTGCTACAGCAGGTAGAGGGAAGCCTAAGCTTGCTTGGTGAAACTGCCAGTATTGTGGGTTATGAGATCCA
CTGCGGCCGCTCACGCCAGCTAATGCCGCTTGAGCAGCCATTAAGTCTTTGGGCTAAAAATGGTGAGCGAGTTAACCTTG
AACAGCAGCATAGCCAAAGCGAGGGGATGTTATCCAGTGATGGCCAAATCATGGGGACTTATCTACACGGATTATTTGAT
TCGCCCGAGGCATGTCAGTTGATCCTTAAATGGGCGGGTATGGAGCAGGCCGAGGTGGTTGATGTTAATCAAGTGCGTGA
ACAGCAGCTCAACCGACTCGCCGATGTGCTTGAGCAGCACTTAGATCTCGAACGTCTGAATCAGATCTGGGATCCCCGTT
AG

Upstream 100 bases:

>100_bases
AGCGGGCTGGTTGCATCAAGAGATAGCGGCAATTGCAGACAGAGTCACCTTAGTGACTGCTGGTTTGCCTCTGTGTTTAA
AAGGTTAGGAGAGTGCCAAA

Downstream 100 bases:

>100_bases
GGATTGGGCTAACGTTAGGCTTAATAACGAGGTGAGTGCATACAGAGTATCGTGAATAAGTCGAAAAATGTATTTATAAG
AGCTAAACAGGCCAAAAGCG

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 533; Mature: 532

Protein sequence:

>533_residues
MTVKVAVTDNNNANNLTKVLMVQGTTSDAGKSTLVAGLCRLFVRQGVKVAPFKPQNMALNSAVTIDGGEIGRAQALQAVA
CHLEPQIDFNPILLKPSSDTGSQVIVHGKALMKMEAEDYFGKNAEGYRVKAMAAVRESYLRLTSEYDMLLVEGAGSPAEI
NLREGDIANMGFAEEVDCPVIIIADIDKGGVFAHLVGTLALLSESEQARVKGFVINRFRGDVSLLQGGLDWLESYTNKPV
LGVLPYLHDLHLDAEDALIAAPEKSNDTKLKVLVLVLPRISNHTDFDPLRLNPQIDFNYVSLQTQAQSQSQNQSQSKQAL
PSADLIIIPGSKNVRADLAFIREQGWDCAIDKHLRYGGKVLGICGGYQILGQSIDDPDGIEDYAGSSEALGLLPLVTELT
STKVLQQVEGSLSLLGETASIVGYEIHCGRSRQLMPLEQPLSLWAKNGERVNLEQQHSQSEGMLSSDGQIMGTYLHGLFD
SPEACQLILKWAGMEQAEVVDVNQVREQQLNRLADVLEQHLDLERLNQIWDPR

Sequences:

>Translated_533_residues
MTVKVAVTDNNNANNLTKVLMVQGTTSDAGKSTLVAGLCRLFVRQGVKVAPFKPQNMALNSAVTIDGGEIGRAQALQAVA
CHLEPQIDFNPILLKPSSDTGSQVIVHGKALMKMEAEDYFGKNAEGYRVKAMAAVRESYLRLTSEYDMLLVEGAGSPAEI
NLREGDIANMGFAEEVDCPVIIIADIDKGGVFAHLVGTLALLSESEQARVKGFVINRFRGDVSLLQGGLDWLESYTNKPV
LGVLPYLHDLHLDAEDALIAAPEKSNDTKLKVLVLVLPRISNHTDFDPLRLNPQIDFNYVSLQTQAQSQSQNQSQSKQAL
PSADLIIIPGSKNVRADLAFIREQGWDCAIDKHLRYGGKVLGICGGYQILGQSIDDPDGIEDYAGSSEALGLLPLVTELT
STKVLQQVEGSLSLLGETASIVGYEIHCGRSRQLMPLEQPLSLWAKNGERVNLEQQHSQSEGMLSSDGQIMGTYLHGLFD
SPEACQLILKWAGMEQAEVVDVNQVREQQLNRLADVLEQHLDLERLNQIWDPR
>Mature_532_residues
TVKVAVTDNNNANNLTKVLMVQGTTSDAGKSTLVAGLCRLFVRQGVKVAPFKPQNMALNSAVTIDGGEIGRAQALQAVAC
HLEPQIDFNPILLKPSSDTGSQVIVHGKALMKMEAEDYFGKNAEGYRVKAMAAVRESYLRLTSEYDMLLVEGAGSPAEIN
LREGDIANMGFAEEVDCPVIIIADIDKGGVFAHLVGTLALLSESEQARVKGFVINRFRGDVSLLQGGLDWLESYTNKPVL
GVLPYLHDLHLDAEDALIAAPEKSNDTKLKVLVLVLPRISNHTDFDPLRLNPQIDFNYVSLQTQAQSQSQNQSQSKQALP
SADLIIIPGSKNVRADLAFIREQGWDCAIDKHLRYGGKVLGICGGYQILGQSIDDPDGIEDYAGSSEALGLLPLVTELTS
TKVLQQVEGSLSLLGETASIVGYEIHCGRSRQLMPLEQPLSLWAKNGERVNLEQQHSQSEGMLSSDGQIMGTYLHGLFDS
PEACQLILKWAGMEQAEVVDVNQVREQQLNRLADVLEQHLDLERLNQIWDPR

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.10

Molecular weight: Translated: 58220; Mature: 58089

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKVAVTDNNNANNLTKVLMVQGTTSDAGKSTLVAGLCRLFVRQGVKVAPFKPQNMALN
CEEEEEEECCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCEEC
SAVTIDGGEIGRAQALQAVACHLEPQIDFNPILLKPSSDTGSQVIVHGKALMKMEAEDYF
CEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCEEEEECHHHEEEEHHHHC
GKNAEGYRVKAMAAVRESYLRLTSEYDMLLVEGAGSPAEINLREGDIANMGFAEEVDCPV
CCCCCCEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCE
IIIADIDKGGVFAHLVGTLALLSESEQARVKGFVINRFRGDVSLLQGGLDWLESYTNKPV
EEEEECCCCCHHHHHHHHHHHHCCCCHHHHHEEEEEHHCCCHHHHHHHHHHHHHHCCCCH
LGVLPYLHDLHLDAEDALIAAPEKSNDTKLKVLVLVLPRISNHTDFDPLRLNPQIDFNYV
HHHHHHHHHHCCCCCCCEEECCCCCCCCEEEEEEEEECCCCCCCCCCCEEECCEECEEEE
SLQTQAQSQSQNQSQSKQALPSADLIIIPGSKNVRADLAFIREQGWDCAIDKHLRYGGKV
EEEEHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHCCCCEEHHHHHHCCCEE
LGICGGYQILGQSIDDPDGIEDYAGSSEALGLLPLVTELTSTKVLQQVEGSLSLLGETAS
EEEECCHHHHCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHCCCHH
IVGYEIHCGRSRQLMPLEQPLSLWAKNGERVNLEQQHSQSEGMLSSDGQIMGTYLHGLFD
EEEEEEECCCCCCCCCHHHHHHHHHCCCCEECHHHHCCCCCCCCCCCCCEEEEHHHHHCC
SPEACQLILKWAGMEQAEVVDVNQVREQQLNRLADVLEQHLDLERLNQIWDPR
CHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCC
>Mature Secondary Structure 
TVKVAVTDNNNANNLTKVLMVQGTTSDAGKSTLVAGLCRLFVRQGVKVAPFKPQNMALN
EEEEEEECCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCEEC
SAVTIDGGEIGRAQALQAVACHLEPQIDFNPILLKPSSDTGSQVIVHGKALMKMEAEDYF
CEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCEEEEECHHHEEEEHHHHC
GKNAEGYRVKAMAAVRESYLRLTSEYDMLLVEGAGSPAEINLREGDIANMGFAEEVDCPV
CCCCCCEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCE
IIIADIDKGGVFAHLVGTLALLSESEQARVKGFVINRFRGDVSLLQGGLDWLESYTNKPV
EEEEECCCCCHHHHHHHHHHHHCCCCHHHHHEEEEEHHCCCHHHHHHHHHHHHHHCCCCH
LGVLPYLHDLHLDAEDALIAAPEKSNDTKLKVLVLVLPRISNHTDFDPLRLNPQIDFNYV
HHHHHHHHHHCCCCCCCEEECCCCCCCCEEEEEEEEECCCCCCCCCCCEEECCEECEEEE
SLQTQAQSQSQNQSQSKQALPSADLIIIPGSKNVRADLAFIREQGWDCAIDKHLRYGGKV
EEEEHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHCCCCEEHHHHHHCCCEE
LGICGGYQILGQSIDDPDGIEDYAGSSEALGLLPLVTELTSTKVLQQVEGSLSLLGETAS
EEEECCHHHHCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHCCCHH
IVGYEIHCGRSRQLMPLEQPLSLWAKNGERVNLEQQHSQSEGMLSSDGQIMGTYLHGLFD
EEEEEEECCCCCCCCCHHHHHHHHHCCCCEECHHHHCCCCCCCCCCCCCEEEEHHHHHCC
SPEACQLILKWAGMEQAEVVDVNQVREQQLNRLADVLEQHLDLERLNQIWDPR
CHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]