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The map label for this gene is btuF [H]

Identifier: 167625363

GI number: 167625363

Start: 4206148

End: 4206984

Strand: Reverse

Name: btuF [H]

Synonym: Shal_3452

Alternate gene names: 167625363

Gene position: 4206984-4206148 (Counterclockwise)

Preceding gene: 167625364

Following gene: 167625362

Centisome position: 80.49

GC content: 49.1

Gene sequence:

>837_bases
ATGAACAGAGTTACCCAATTATTTATGTTGCTGACGGCATGCGCCTTACCGTCGGCTTTTGCGCTTGCTGATAACGCTGC
GCCCGCTAAACGTATTGTTGCGCTTTCGCCACATTCGGTTGAAATGCTGTATGCGATTGGGGCGGGTGAGTCGATTGTAG
CCACTACCGACCATGCTGACTTTCCTGAGGCCGCACTGCAAATTCCACGTATTGGCGGTTATTACGGCATTCAGATCGAG
CGGGTGCTGGAGTTAGAGCCTGATCTTGTAGTGGTATGGAGTGGTGGCAATAAGGCTGAAGATATTGCACGCATCAAAGA
GTTGGGCTTTCGTGTGTTTGATAGCAATCCGAGTACGCTTGAGCTGGTGGCCGATGAGCTCGTCTCTCTTGGTGAGCTAA
CGGGTCATCAAGCTCAAGCGACAAAGGTTGCCGATGATTACCGACAGCAGCTTAGTCAGCTGCGCAGTGACAACAGTGTT
AAACCTGGGGTCAGTGTGTTTTATCAGTTATGGTCTACGCCACTTATGACTGTCGCTAAGAACAGCTGGATCCAACAGAT
CATCGGTGTATGCCATGGTGATAATGTATTTGTCGATGCAGCTAATGATTACCCACAAGTCAGCCTAGAAACTGTGGTAC
TGAAACAACCTGAAGTTATTCTGCAAAGCCAAGATGAAGGCAACATCATAGGCGTGGATTGGAGCCTGTGGACAGAGATC
CCAGCCGTTAAAAATGAGCACATCTACCAACTCAACGCGGATCTTTTACATCGCGCCGCGCCTAGGGCTTTGCTTGGCGT
TGAAGCACTTTGTGAAGCGTTAGATAAAGTGCGTTAA

Upstream 100 bases:

>100_bases
CCAGCTTTTTCATCTTTGCTGTTGTCGGCCTTTCCGTCTTGGCTTGAGTCGTCTTAGCTCGTACCGTCTTAGATTTAACC
TATTTTCAAAAAGGATTTTA

Downstream 100 bases:

>100_bases
ACGTTAGCGACTCAGTTCGCAAGCCTGTTCGATTCAGGCTTGCTGCACCACGAAACTACTCCATGAACGCCTATAATCCA
CTATATTTTTTGGTAAGTAC

Product: periplasmic-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MNRVTQLFMLLTACALPSAFALADNAAPAKRIVALSPHSVEMLYAIGAGESIVATTDHADFPEAALQIPRIGGYYGIQIE
RVLELEPDLVVVWSGGNKAEDIARIKELGFRVFDSNPSTLELVADELVSLGELTGHQAQATKVADDYRQQLSQLRSDNSV
KPGVSVFYQLWSTPLMTVAKNSWIQQIIGVCHGDNVFVDAANDYPQVSLETVVLKQPEVILQSQDEGNIIGVDWSLWTEI
PAVKNEHIYQLNADLLHRAAPRALLGVEALCEALDKVR

Sequences:

>Translated_278_residues
MNRVTQLFMLLTACALPSAFALADNAAPAKRIVALSPHSVEMLYAIGAGESIVATTDHADFPEAALQIPRIGGYYGIQIE
RVLELEPDLVVVWSGGNKAEDIARIKELGFRVFDSNPSTLELVADELVSLGELTGHQAQATKVADDYRQQLSQLRSDNSV
KPGVSVFYQLWSTPLMTVAKNSWIQQIIGVCHGDNVFVDAANDYPQVSLETVVLKQPEVILQSQDEGNIIGVDWSLWTEI
PAVKNEHIYQLNADLLHRAAPRALLGVEALCEALDKVR
>Mature_278_residues
MNRVTQLFMLLTACALPSAFALADNAAPAKRIVALSPHSVEMLYAIGAGESIVATTDHADFPEAALQIPRIGGYYGIQIE
RVLELEPDLVVVWSGGNKAEDIARIKELGFRVFDSNPSTLELVADELVSLGELTGHQAQATKVADDYRQQLSQLRSDNSV
KPGVSVFYQLWSTPLMTVAKNSWIQQIIGVCHGDNVFVDAANDYPQVSLETVVLKQPEVILQSQDEGNIIGVDWSLWTEI
PAVKNEHIYQLNADLLHRAAPRALLGVEALCEALDKVR

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=273, Percent_Identity=32.2344322344322, Blast_Score=143, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 30429; Mature: 30429

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRVTQLFMLLTACALPSAFALADNAAPAKRIVALSPHSVEMLYAIGAGESIVATTDHAD
CCHHHHHHHHHHHHHCCHHHHHCCCCCCCCEEEEECCCCEEEEEEECCCCEEEEECCCCC
FPEAALQIPRIGGYYGIQIERVLELEPDLVVVWSGGNKAEDIARIKELGFRVFDSNPSTL
CCHHHHCCCCCCCEECEEEEEEEECCCCEEEEECCCCCHHHHHHHHHCCCEEECCCCCHH
ELVADELVSLGELTGHQAQATKVADDYRQQLSQLRSDNSVKPGVSVFYQLWSTPLMTVAK
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHH
NSWIQQIIGVCHGDNVFVDAANDYPQVSLETVVLKQPEVILQSQDEGNIIGVDWSLWTEI
HHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEECCCCEEEECCCCCCEEEECHHHHHCC
PAVKNEHIYQLNADLLHRAAPRALLGVEALCEALDKVR
CCCCCCEEEEECHHHHHHHCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNRVTQLFMLLTACALPSAFALADNAAPAKRIVALSPHSVEMLYAIGAGESIVATTDHAD
CCHHHHHHHHHHHHHCCHHHHHCCCCCCCCEEEEECCCCEEEEEEECCCCEEEEECCCCC
FPEAALQIPRIGGYYGIQIERVLELEPDLVVVWSGGNKAEDIARIKELGFRVFDSNPSTL
CCHHHHCCCCCCCEECEEEEEEEECCCCEEEEECCCCCHHHHHHHHHCCCEEECCCCCHH
ELVADELVSLGELTGHQAQATKVADDYRQQLSQLRSDNSVKPGVSVFYQLWSTPLMTVAK
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHH
NSWIQQIIGVCHGDNVFVDAANDYPQVSLETVVLKQPEVILQSQDEGNIIGVDWSLWTEI
HHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEECCCCEEEECCCCCCEEEECHHHHHCC
PAVKNEHIYQLNADLLHRAAPRALLGVEALCEALDKVR
CCCCCCEEEEECHHHHHHHCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA