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The map label for this gene is gcvH

Identifier: 167625285

GI number: 167625285

Start: 4117455

End: 4117844

Strand: Reverse

Name: gcvH

Synonym: Shal_3374

Alternate gene names: 167625285

Gene position: 4117844-4117455 (Counterclockwise)

Preceding gene: 167625286

Following gene: 167625284

Centisome position: 78.78

GC content: 47.69

Gene sequence:

>390_bases
ATGAGTAATATCCCAGCTGAACTTAAATATGCTTCTTCTCATGAGTGGGTACGTAAAGAAGAAGACGGTAGCTACACAGT
CGGTATCAGTGAGCACGCTCAAGAGCTTTTGGGTGACATGGTGTTCGTTGAGCTGCCAGAAGTAGGCGACGACGTTAACT
CAGGTGAAGACTGCGCAGTAGCAGAGTCTGTAAAAGCGGCATCAGATATCTACGCACCACTTTCAGGCGAAGTCATTGCC
GTTAACGAAGCACTGGAAGATTCTCCTGAGCTAGTCAATAGCGACGCATTCGGTGATGGTTGGTTCTTCCGCGTAATGCC
AACAGACCTTGCAGAGCTAGACAACTTGCTTGATGCCGAAGGTTATCAAGCAGTTATCGACGACGAATAA

Upstream 100 bases:

>100_bases
GATGTAAAAAAGGTGCAAAAGTTGATATAAGCCTTATAGGCAAGTCAGTGTAATGTCTTATTAAATACAGAATTAGAACA
TCGAATAAGGATTTAGACCA

Downstream 100 bases:

>100_bases
GCGTCGATGACTCACAAAGCCCCAACTCATCGCTGATTGGGGCTTTGTTCGTTATATGGCTTATGCCGCTTTTTGCTAGC
CAGAGCATGCAAAAACAAAT

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 129; Mature: 128

Protein sequence:

>129_residues
MSNIPAELKYASSHEWVRKEEDGSYTVGISEHAQELLGDMVFVELPEVGDDVNSGEDCAVAESVKAASDIYAPLSGEVIA
VNEALEDSPELVNSDAFGDGWFFRVMPTDLAELDNLLDAEGYQAVIDDE

Sequences:

>Translated_129_residues
MSNIPAELKYASSHEWVRKEEDGSYTVGISEHAQELLGDMVFVELPEVGDDVNSGEDCAVAESVKAASDIYAPLSGEVIA
VNEALEDSPELVNSDAFGDGWFFRVMPTDLAELDNLLDAEGYQAVIDDE
>Mature_128_residues
SNIPAELKYASSHEWVRKEEDGSYTVGISEHAQELLGDMVFVELPEVGDDVNSGEDCAVAESVKAASDIYAPLSGEVIAV
NEALEDSPELVNSDAFGDGWFFRVMPTDLAELDNLLDAEGYQAVIDDE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=120, Percent_Identity=47.5, Blast_Score=115, Evalue=1e-26,
Organism=Homo sapiens, GI89057342, Length=120, Percent_Identity=47.5, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1789271, Length=129, Percent_Identity=68.9922480620155, Blast_Score=194, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17507493, Length=120, Percent_Identity=45, Blast_Score=104, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17551294, Length=120, Percent_Identity=42.5, Blast_Score=103, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319272, Length=125, Percent_Identity=38.4, Blast_Score=102, Evalue=2e-23,
Organism=Drosophila melanogaster, GI17865652, Length=125, Percent_Identity=43.2, Blast_Score=112, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_SHEHH (B0TSG6)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001675579.1
- ProteinModelPortal:   B0TSG6
- SMR:   B0TSG6
- GeneID:   5903482
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_3374
- HOGENOM:   HBG693789
- OMA:   SASEYEQ
- ProtClustDB:   PRK01202
- BioCyc:   SHAL458817:SHAL_3374-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13986; Mature: 13855

Theoretical pI: Translated: 3.51; Mature: 3.51

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIPAELKYASSHEWVRKEEDGSYTVGISEHAQELLGDMVFVELPEVGDDVNSGEDCAV
CCCCCCCEEECCCCCCCEECCCCCEEEEHHHHHHHHHHCEEEEECCCCCCCCCCCCCCHH
AESVKAASDIYAPLSGEVIAVNEALEDSPELVNSDAFGDGWFFRVMPTDLAELDNLLDAE
HHHHHHHHHHCCCCCCCEEEECCCCCCCCHHCCCCCCCCCEEEEECCCCHHHHHHHHCCC
GYQAVIDDE
CCEEEECCC
>Mature Secondary Structure 
SNIPAELKYASSHEWVRKEEDGSYTVGISEHAQELLGDMVFVELPEVGDDVNSGEDCAV
CCCCCCEEECCCCCCCEECCCCCEEEEHHHHHHHHHHCEEEEECCCCCCCCCCCCCCHH
AESVKAASDIYAPLSGEVIAVNEALEDSPELVNSDAFGDGWFFRVMPTDLAELDNLLDAE
HHHHHHHHHHCCCCCCCEEEECCCCCCCCHHCCCCCCCCCEEEEECCCCHHHHHHHHCCC
GYQAVIDDE
CCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA