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The map label for this gene is gcvT

Identifier: 167625286

GI number: 167625286

Start: 4118267

End: 4119361

Strand: Reverse

Name: gcvT

Synonym: Shal_3375

Alternate gene names: 167625286

Gene position: 4119361-4118267 (Counterclockwise)

Preceding gene: 167625287

Following gene: 167625285

Centisome position: 78.81

GC content: 50.32

Gene sequence:

>1095_bases
ATGGCTAACAAAACTGTACTTTTTAACAAGCATCTAGAATCTAACGGCAAGATGGTAGACTTCCACGGTTGGGACATGCC
ACTTAACTACGGTTCACAAATCGAAGAGCATCACGCGGTGCGCCAAGACGCAGGTATGTTCGACGTTTCTCACATGACTG
TAGTTGATGTGATTGGTGATGACGCTTGTGCCTTTTTACGCAAACTGCTAGCCAACGACGTCGCTAAGCTTAAAGTCCCT
GGTAAGGCCCTATATGGCGGTATGCTTGACCATAACGGCGGCGTCATCGACGACCTTATCACTTATTACCTATCTGATAC
CGAGTACCGTATTGTTGTTAACTCAGCAACACGCGAAAAAGACCTCGCTTGGATAAACGAGCAAGTTAACGGTTTCAGCG
TTGAAGTGACCGAGCGTCCAGAGCTGGCGATGATCGCCGTTCAAGGTCCAAACGCTAAGGCAAAAGCCGCCACCGTATTT
AACGCCGAGCAAAATGCCGCGATTGAAGGCATGAAGCCATTCTTCGGTGTGCAAACTGGCTCGCTGTTTATCGCAACCAC
AGGTTACACTGGCGAAACGGGCTACGAAGTCATCGTGCCAGAAGCCGATGCCGAAGCACTATGGCAAGCTTTCCTAGAAG
CGGGCATTAAGCCATGTGGACTCGGTGCACGTGACACTCTACGTTTAGAGGCGGGCATGAACCTTTACGGCCTAGATATG
GACGAAAGTGTTAATCCACTCGCCGCGAACATGGGCTGGACTGTGGCATGGGAGCCAGCGGATCGTGACTTTAACGGTCG
CCAAGCGTTAGAGAAGATTAAAGCCGAAGGTACCGACAAGCTAGTGGGCCTCATCATGGACGCTAAAGGCGTTATCCGTC
ACGGCATGTCAGTGTTCTTTACCGACAGCGATGGTGTTGAGCAGCAAGGTATCATCACCAGTGGTACATTCTCGCCTACA
CTAGGTTACTCAATTGCCATGGCTCGCGTACCGCGCAGCATTGGTGATGTTGCCGAAGTAGAGATGCGTAAGAAGCGTGT
ACCCGTTAAGGTTATCGCTCCATCATTTGTGCGCAATGGTAAACAAGCATTCTAA

Upstream 100 bases:

>100_bases
AGTGCCTTATAGTCGGTTTCTTATAGTAAGTACCTTTATTCGTACCCTATATAAAGTGATGGCAGCATACAGACTAGAAG
ACCCCAAAGAAGGGATAGAA

Downstream 100 bases:

>100_bases
TTATATTGGTGGGTTTATAGCTATTTGCATTGGCTTCGTTGTCGAAAGTGCTCATTGATGCTTATCAACTGCGCGCTTTC
TCCTAGAATCCAAAGCAACT

Product: glycine cleavage system aminomethyltransferase T

Products: NA

Alternate protein names: Glycine cleavage system T protein

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MANKTVLFNKHLESNGKMVDFHGWDMPLNYGSQIEEHHAVRQDAGMFDVSHMTVVDVIGDDACAFLRKLLANDVAKLKVP
GKALYGGMLDHNGGVIDDLITYYLSDTEYRIVVNSATREKDLAWINEQVNGFSVEVTERPELAMIAVQGPNAKAKAATVF
NAEQNAAIEGMKPFFGVQTGSLFIATTGYTGETGYEVIVPEADAEALWQAFLEAGIKPCGLGARDTLRLEAGMNLYGLDM
DESVNPLAANMGWTVAWEPADRDFNGRQALEKIKAEGTDKLVGLIMDAKGVIRHGMSVFFTDSDGVEQQGIITSGTFSPT
LGYSIAMARVPRSIGDVAEVEMRKKRVPVKVIAPSFVRNGKQAF

Sequences:

>Translated_364_residues
MANKTVLFNKHLESNGKMVDFHGWDMPLNYGSQIEEHHAVRQDAGMFDVSHMTVVDVIGDDACAFLRKLLANDVAKLKVP
GKALYGGMLDHNGGVIDDLITYYLSDTEYRIVVNSATREKDLAWINEQVNGFSVEVTERPELAMIAVQGPNAKAKAATVF
NAEQNAAIEGMKPFFGVQTGSLFIATTGYTGETGYEVIVPEADAEALWQAFLEAGIKPCGLGARDTLRLEAGMNLYGLDM
DESVNPLAANMGWTVAWEPADRDFNGRQALEKIKAEGTDKLVGLIMDAKGVIRHGMSVFFTDSDGVEQQGIITSGTFSPT
LGYSIAMARVPRSIGDVAEVEMRKKRVPVKVIAPSFVRNGKQAF
>Mature_363_residues
ANKTVLFNKHLESNGKMVDFHGWDMPLNYGSQIEEHHAVRQDAGMFDVSHMTVVDVIGDDACAFLRKLLANDVAKLKVPG
KALYGGMLDHNGGVIDDLITYYLSDTEYRIVVNSATREKDLAWINEQVNGFSVEVTERPELAMIAVQGPNAKAKAATVFN
AEQNAAIEGMKPFFGVQTGSLFIATTGYTGETGYEVIVPEADAEALWQAFLEAGIKPCGLGARDTLRLEAGMNLYGLDMD
ESVNPLAANMGWTVAWEPADRDFNGRQALEKIKAEGTDKLVGLIMDAKGVIRHGMSVFFTDSDGVEQQGIITSGTFSPTL
GYSIAMARVPRSIGDVAEVEMRKKRVPVKVIAPSFVRNGKQAF

Specific function: The glycine cleavage system catalyzes the degradation of glycine

COG id: COG0404

COG function: function code E; Glycine cleavage system T protein (aminomethyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family

Homologues:

Organism=Homo sapiens, GI44662838, Length=364, Percent_Identity=32.967032967033, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI257796258, Length=346, Percent_Identity=33.2369942196532, Blast_Score=164, Evalue=8e-41,
Organism=Homo sapiens, GI257796254, Length=357, Percent_Identity=29.4117647058824, Blast_Score=131, Evalue=8e-31,
Organism=Homo sapiens, GI257796256, Length=309, Percent_Identity=31.3915857605178, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI24797151, Length=341, Percent_Identity=30.2052785923754, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI197927446, Length=295, Percent_Identity=26.4406779661017, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI21361378, Length=295, Percent_Identity=26.4406779661017, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1789272, Length=361, Percent_Identity=66.4819944598338, Blast_Score=511, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI17560118, Length=378, Percent_Identity=33.3333333333333, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI71994045, Length=354, Percent_Identity=24.0112994350282, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71994052, Length=354, Percent_Identity=24.0112994350282, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI32563613, Length=312, Percent_Identity=23.3974358974359, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6320222, Length=375, Percent_Identity=33.6, Blast_Score=166, Evalue=5e-42,
Organism=Drosophila melanogaster, GI20129441, Length=377, Percent_Identity=33.1564986737401, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI28571104, Length=270, Percent_Identity=26.2962962962963, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI20130091, Length=368, Percent_Identity=23.9130434782609, Blast_Score=75, Evalue=9e-14,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): GCST_SHEHH (B0TSG7)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001675580.1
- ProteinModelPortal:   B0TSG7
- SMR:   B0TSG7
- GeneID:   5903483
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_3375
- HOGENOM:   HBG299834
- OMA:   KALYGGM
- ProtClustDB:   PRK00389
- BioCyc:   SHAL458817:SHAL_3375-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00259
- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903
- PIRSF:   PIRSF006487
- TIGRFAMs:   TIGR00528

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C

EC number: =2.1.2.10

Molecular weight: Translated: 39646; Mature: 39514

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANKTVLFNKHLESNGKMVDFHGWDMPLNYGSQIEEHHAVRQDAGMFDVSHMTVVDVIGD
CCCCEEEEECEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEECCEEEEEECCC
DACAFLRKLLANDVAKLKVPGKALYGGMLDHNGGVIDDLITYYLSDTEYRIVVNSATREK
HHHHHHHHHHHCCHHEEECCCHHHCCCEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCH
DLAWINEQVNGFSVEVTERPELAMIAVQGPNAKAKAATVFNAEQNAAIEGMKPFFGVQTG
HHHHHHHCCCCEEEEEECCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCHHEECCC
SLFIATTGYTGETGYEVIVPEADAEALWQAFLEAGIKPCGLGARDTLRLEAGMNLYGLDM
CEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEECCC
DESVNPLAANMGWTVAWEPADRDFNGRQALEKIKAEGTDKLVGLIMDAKGVIRHGMSVFF
CCCCCCHHHCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEECCHHHHHCCCEEEE
TDSDGVEQQGIITSGTFSPTLGYSIAMARVPRSIGDVAEVEMRKKRVPVKVIAPSFVRNG
ECCCCCCCCCEEECCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCEEEECHHHHCCC
KQAF
CCCC
>Mature Secondary Structure 
ANKTVLFNKHLESNGKMVDFHGWDMPLNYGSQIEEHHAVRQDAGMFDVSHMTVVDVIGD
CCCEEEEECEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEECCEEEEEECCC
DACAFLRKLLANDVAKLKVPGKALYGGMLDHNGGVIDDLITYYLSDTEYRIVVNSATREK
HHHHHHHHHHHCCHHEEECCCHHHCCCEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCH
DLAWINEQVNGFSVEVTERPELAMIAVQGPNAKAKAATVFNAEQNAAIEGMKPFFGVQTG
HHHHHHHCCCCEEEEEECCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCHHEECCC
SLFIATTGYTGETGYEVIVPEADAEALWQAFLEAGIKPCGLGARDTLRLEAGMNLYGLDM
CEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEECCC
DESVNPLAANMGWTVAWEPADRDFNGRQALEKIKAEGTDKLVGLIMDAKGVIRHGMSVFF
CCCCCCHHHCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEECCHHHHHCCCEEEE
TDSDGVEQQGIITSGTFSPTLGYSIAMARVPRSIGDVAEVEMRKKRVPVKVIAPSFVRNG
ECCCCCCCCCEEECCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCEEEECHHHHCCC
KQAF
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA