Click here to switch to the map view.

The map label for this gene is phsA [H]

Identifier: 167625183

GI number: 167625183

Start: 3988843

End: 3991113

Strand: Reverse

Name: phsA [H]

Synonym: Shal_3271

Alternate gene names: 167625183

Gene position: 3991113-3988843 (Counterclockwise)

Preceding gene: 167625184

Following gene: 167625182

Centisome position: 76.36

GC content: 48.57

Gene sequence:

>2271_bases
ATGTTGTCTCGCCGCGATTTTTTGGCAAAAGGTAGCGCCTTTTGCGCTATAACAATTTATGCCCCCGGAACTTTAGGGGC
GCAAAGTCAGTTATTAACACCCGCTACGCCATTTGTTAAAGAAATAAACAGTATCTGCGAAATGTGTTCAACTCGATGTC
CAATTACCGTCGAAAGCAGACAAGATAAGTTGTTTATTCGGGGGAACCTGAACGCTAAGAGCTTTGGTGGTAAGGTATGC
GCAAGAGGCACATCAGGTAGCAAGCTACTTAATGATCCTCACCGTTTAGTTAAACCGATTAAACGAACCGGACCTCGAGG
CTCGGGAATGTGGCAGGAAATAGAGTGGGAGGAAGCATACCAACTTATTGCTCACAACCTGAATCAAGTTTGTGATCGAT
ACGGACCTGAAGCAATCGCAGTCTCGAGTAAATCGGGCTCATTATCCAGCCATGCCTTTCACTTCGGTAAAGTTATCGGT
ACTCCAAATATTTTTTCACACGGCTCAACATGTCCAATTTCCTATGCCGTCGCTGCTAAGGTGATGTTCGGTAATGAGCT
TAAACTCGATTTAGAAAACAGCAAATATATACTCAACTTTGGTCATAACCTGTTCGAAGGGATCAACATGTCAATGACCC
GGGGCTTGTTAGCTGCACAGGAGAAAGGCGCAGTTAAAGTTGTGGTGTTTGAACCTAGATTATCGGTTATGGCAGACAAA
GCGGATGAATGGCATGCCATTAAACCCGGCACCGACATTGCCGTTGTACTCGCTCTGAGCCATGTATTGATTGAGGAGCA
GTTGTACGACAAATCCTTTGTGCAACAACACGTCAGTGGCTTCGAGGGTTTTGCGACTTCGATGGCACAATATAGCCCTG
AATGGGCAAGTGAGATATGTGACGTGTCAGCAGGCGACATTCGTCGTATCGCCCGAGAACTTGCCGCAGCTGCCCCCGCT
GCGTTAGCCGACCATGGGCATCGTTGCACCTTTACACCTGAAGAGTTCGATCTTCGACGTGGTATATATGCCATAAACGT
ACTGCTGGGTAATATTGAACGTAAAGGAGGGGTGTATTTCGCTAAGAAATCTAAGCTGTATAACAAGCTGGCTGGCGAAG
CGGTAGCCCCGTCACTGGAAAAAATAAAAGTTGATGGCTTACCAACCCCAGTCTTACCACGCATCGATTTAGCCGAACCT
CAGTTTAAGTATGCCGCCAGAGCGGGTGGGCTCTATCAATCTATTATCCCTGCAGCCTTGCTGCAACAGCCCTACGCGAT
CAAGGCATGGGTGATGTCACGCTCTAACCCGATGCAAACCGTCACTGATCGAACCCAAGTTCAAAATGCACTCGAGGCGA
TGGATTTTGTTGTCGCCTGTGACGTTTATATCAGCGAGAGTGCGGCCTATGCTGACGTTGTTTTGCCTGAATCCACTTAC
CTTGAGCGCGATGAAGAGATCGGTGATATGTCTTCCAAGAATCCCGCTTATGCCCTGCGACAGCGAGTCACAGAAACTAT
CGGTGATACTCGTCCAAGTTGGCAAATATTTAAAGAGATGGCAGACGCAATGGCGTTGGGGCACCACTTCCCCTGGCAAG
ATATTGTTGACTACCAACAACGCCAGACTGCAAACAACCCTGAGTTATTACCGCAGCTTAGAAAAGAAGGTTTCCTCTCT
TTTGGCATCCCACTGTTGATGCGAGAACCAGATATGGTGGCGAAGTTTCGCCAACATTACCCTAATGCGCAGCCTCCATC
TGAGCGTGGCGACTACGGAAATCAGCTTCAATTTAGCACGCCAAGTGGCAAGATTGAATTGACCTCTCCTAGTCTCGAAG
AGATAGCGCCTGGCCGCAGTGTGATTAGCTACCGCCCCGTGACGCTAAAGCAGCCTAACGAGCTGTACTTTATCCAAGGA
AAGTCAGCATTGCACACCAACGCAGCAACTCAAAATATCCCGCTGCTCTTTGATTTGCAGCCCGATAACCCTGTCTGGAT
CCACCCTGACACAGCTGCAGAATTTAGGATAAATGATGGCGATAGCATCACTATGACGAGTCAAGTGGGAGAGGAGCAAG
GCACTGCCAAGCTAACACTTGGGATTCGTAAAGACACCGTTTTCGCACATATGGGCTTTGGTAGTAAGAACCAAGAGCTC
ACACGCGCCTTCGGTAAGGGATTACATTGCGGCGCCTTATTACCCGATGCCACCGCCGCTATTACCGGCAGTAATGTTCA
TACCACGGGCGTCACGATCAGACGCGCGTAA

Upstream 100 bases:

>100_bases
TGAACTGAGCTTACTATGCACACAGCTAGTCTCTAGTCCTAAAAATAAAAAAATTGCTTTTAAATCTGATGTTTATGAAT
GTGAAGAGAGAGGTAAGAAC

Downstream 100 bases:

>100_bases
TGTGTATTGAGTTTAACTAACAAGGAGCGACTTTACTCCAGCCAATGCAATAGCAAGTGCTGGAGTAGAGTGGGAGAAAC
GCAATGAACGAAAAATTTGT

Product: nitrate reductase

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 756; Mature: 756

Protein sequence:

>756_residues
MLSRRDFLAKGSAFCAITIYAPGTLGAQSQLLTPATPFVKEINSICEMCSTRCPITVESRQDKLFIRGNLNAKSFGGKVC
ARGTSGSKLLNDPHRLVKPIKRTGPRGSGMWQEIEWEEAYQLIAHNLNQVCDRYGPEAIAVSSKSGSLSSHAFHFGKVIG
TPNIFSHGSTCPISYAVAAKVMFGNELKLDLENSKYILNFGHNLFEGINMSMTRGLLAAQEKGAVKVVVFEPRLSVMADK
ADEWHAIKPGTDIAVVLALSHVLIEEQLYDKSFVQQHVSGFEGFATSMAQYSPEWASEICDVSAGDIRRIARELAAAAPA
ALADHGHRCTFTPEEFDLRRGIYAINVLLGNIERKGGVYFAKKSKLYNKLAGEAVAPSLEKIKVDGLPTPVLPRIDLAEP
QFKYAARAGGLYQSIIPAALLQQPYAIKAWVMSRSNPMQTVTDRTQVQNALEAMDFVVACDVYISESAAYADVVLPESTY
LERDEEIGDMSSKNPAYALRQRVTETIGDTRPSWQIFKEMADAMALGHHFPWQDIVDYQQRQTANNPELLPQLRKEGFLS
FGIPLLMREPDMVAKFRQHYPNAQPPSERGDYGNQLQFSTPSGKIELTSPSLEEIAPGRSVISYRPVTLKQPNELYFIQG
KSALHTNAATQNIPLLFDLQPDNPVWIHPDTAAEFRINDGDSITMTSQVGEEQGTAKLTLGIRKDTVFAHMGFGSKNQEL
TRAFGKGLHCGALLPDATAAITGSNVHTTGVTIRRA

Sequences:

>Translated_756_residues
MLSRRDFLAKGSAFCAITIYAPGTLGAQSQLLTPATPFVKEINSICEMCSTRCPITVESRQDKLFIRGNLNAKSFGGKVC
ARGTSGSKLLNDPHRLVKPIKRTGPRGSGMWQEIEWEEAYQLIAHNLNQVCDRYGPEAIAVSSKSGSLSSHAFHFGKVIG
TPNIFSHGSTCPISYAVAAKVMFGNELKLDLENSKYILNFGHNLFEGINMSMTRGLLAAQEKGAVKVVVFEPRLSVMADK
ADEWHAIKPGTDIAVVLALSHVLIEEQLYDKSFVQQHVSGFEGFATSMAQYSPEWASEICDVSAGDIRRIARELAAAAPA
ALADHGHRCTFTPEEFDLRRGIYAINVLLGNIERKGGVYFAKKSKLYNKLAGEAVAPSLEKIKVDGLPTPVLPRIDLAEP
QFKYAARAGGLYQSIIPAALLQQPYAIKAWVMSRSNPMQTVTDRTQVQNALEAMDFVVACDVYISESAAYADVVLPESTY
LERDEEIGDMSSKNPAYALRQRVTETIGDTRPSWQIFKEMADAMALGHHFPWQDIVDYQQRQTANNPELLPQLRKEGFLS
FGIPLLMREPDMVAKFRQHYPNAQPPSERGDYGNQLQFSTPSGKIELTSPSLEEIAPGRSVISYRPVTLKQPNELYFIQG
KSALHTNAATQNIPLLFDLQPDNPVWIHPDTAAEFRINDGDSITMTSQVGEEQGTAKLTLGIRKDTVFAHMGFGSKNQEL
TRAFGKGLHCGALLPDATAAITGSNVHTTGVTIRRA
>Mature_756_residues
MLSRRDFLAKGSAFCAITIYAPGTLGAQSQLLTPATPFVKEINSICEMCSTRCPITVESRQDKLFIRGNLNAKSFGGKVC
ARGTSGSKLLNDPHRLVKPIKRTGPRGSGMWQEIEWEEAYQLIAHNLNQVCDRYGPEAIAVSSKSGSLSSHAFHFGKVIG
TPNIFSHGSTCPISYAVAAKVMFGNELKLDLENSKYILNFGHNLFEGINMSMTRGLLAAQEKGAVKVVVFEPRLSVMADK
ADEWHAIKPGTDIAVVLALSHVLIEEQLYDKSFVQQHVSGFEGFATSMAQYSPEWASEICDVSAGDIRRIARELAAAAPA
ALADHGHRCTFTPEEFDLRRGIYAINVLLGNIERKGGVYFAKKSKLYNKLAGEAVAPSLEKIKVDGLPTPVLPRIDLAEP
QFKYAARAGGLYQSIIPAALLQQPYAIKAWVMSRSNPMQTVTDRTQVQNALEAMDFVVACDVYISESAAYADVVLPESTY
LERDEEIGDMSSKNPAYALRQRVTETIGDTRPSWQIFKEMADAMALGHHFPWQDIVDYQQRQTANNPELLPQLRKEGFLS
FGIPLLMREPDMVAKFRQHYPNAQPPSERGDYGNQLQFSTPSGKIELTSPSLEEIAPGRSVISYRPVTLKQPNELYFIQG
KSALHTNAATQNIPLLFDLQPDNPVWIHPDTAAEFRINDGDSITMTSQVGEEQGTAKLTLGIRKDTVFAHMGFGSKNQEL
TRAFGKGLHCGALLPDATAAITGSNVHTTGVTIRRA

Specific function: Oxidoreductase which produces hydrogen sulfide from thiosulfate (Potential) [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=745, Percent_Identity=25.2348993288591, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1787870, Length=733, Percent_Identity=26.3301500682128, Blast_Score=165, Evalue=9e-42,
Organism=Escherichia coli, GI171474008, Length=775, Percent_Identity=24.9032258064516, Blast_Score=160, Evalue=4e-40,
Organism=Escherichia coli, GI3868721, Length=516, Percent_Identity=26.3565891472868, Blast_Score=155, Evalue=9e-39,
Organism=Escherichia coli, GI145693196, Length=681, Percent_Identity=22.9074889867841, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI87081994, Length=682, Percent_Identity=23.0205278592375, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787231, Length=682, Percent_Identity=21.5542521994135, Blast_Score=94, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 83114; Mature: 83114

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1 ; PS00490 MOLYBDOPTERIN_PROK_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSRRDFLAKGSAFCAITIYAPGTLGAQSQLLTPATPFVKEINSICEMCSTRCPITVESR
CCCCCCHHCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECC
QDKLFIRGNLNAKSFGGKVCARGTSGSKLLNDPHRLVKPIKRTGPRGSGMWQEIEWEEAY
CCEEEEEECCCCCCCCCCEEECCCCCCHHHCCHHHHHHHHHHCCCCCCCCCCCCCHHHHH
QLIAHNLNQVCDRYGPEAIAVSSKSGSLSSHAFHFGKVIGTPNIFSHGSTCPISYAVAAK
HHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
VMFGNELKLDLENSKYILNFGHNLFEGINMSMTRGLLAAQEKGAVKVVVFEPRLSVMADK
EEECCEEEEEECCCEEEEECCCHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHCC
ADEWHAIKPGTDIAVVLALSHVLIEEQLYDKSFVQQHVSGFEGFATSMAQYSPEWASEIC
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
DVSAGDIRRIARELAAAAPAALADHGHRCTFTPEEFDLRRGIYAINVLLGNIERKGGVYF
CCCHHHHHHHHHHHHHHCCHHHHCCCCEECCCCHHHHHHCCCEEEEHHHHCCCCCCCEEE
AKKSKLYNKLAGEAVAPSLEKIKVDGLPTPVLPRIDLAEPQFKYAARAGGLYQSIIPAAL
EHHHHHHHHHCCHHHCCCHHEEEECCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHH
LQQPYAIKAWVMSRSNPMQTVTDRTQVQNALEAMDFVVACDVYISESAAYADVVLPESTY
HCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEECCCCHH
LERDEEIGDMSSKNPAYALRQRVTETIGDTRPSWQIFKEMADAMALGHHFPWQDIVDYQQ
HCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
RQTANNPELLPQLRKEGFLSFGIPLLMREPDMVAKFRQHYPNAQPPSERGDYGNQLQFST
HHCCCCHHHHHHHHHCCCHHCCCHHEECCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEC
PSGKIELTSPSLEEIAPGRSVISYRPVTLKQPNELYFIQGKSALHTNAATQNIPLLFDLQ
CCCEEEECCCCHHHHCCCCCEEEECCEEECCCCEEEEEECCCCEECCCCCCCCCEEEEEC
PDNPVWIHPDTAAEFRINDGDSITMTSQVGEEQGTAKLTLGIRKDTVFAHMGFGSKNQEL
CCCCEEECCCCCEEEEECCCCEEEEEHHCCCCCCCEEEEEEEECCEEEEEECCCCCCHHH
TRAFGKGLHCGALLPDATAAITGSNVHTTGVTIRRA
HHHHCCCCCCCCCCCCCCEEEECCCEEECCEEEEEC
>Mature Secondary Structure
MLSRRDFLAKGSAFCAITIYAPGTLGAQSQLLTPATPFVKEINSICEMCSTRCPITVESR
CCCCCCHHCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECC
QDKLFIRGNLNAKSFGGKVCARGTSGSKLLNDPHRLVKPIKRTGPRGSGMWQEIEWEEAY
CCEEEEEECCCCCCCCCCEEECCCCCCHHHCCHHHHHHHHHHCCCCCCCCCCCCCHHHHH
QLIAHNLNQVCDRYGPEAIAVSSKSGSLSSHAFHFGKVIGTPNIFSHGSTCPISYAVAAK
HHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
VMFGNELKLDLENSKYILNFGHNLFEGINMSMTRGLLAAQEKGAVKVVVFEPRLSVMADK
EEECCEEEEEECCCEEEEECCCHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHCC
ADEWHAIKPGTDIAVVLALSHVLIEEQLYDKSFVQQHVSGFEGFATSMAQYSPEWASEIC
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
DVSAGDIRRIARELAAAAPAALADHGHRCTFTPEEFDLRRGIYAINVLLGNIERKGGVYF
CCCHHHHHHHHHHHHHHCCHHHHCCCCEECCCCHHHHHHCCCEEEEHHHHCCCCCCCEEE
AKKSKLYNKLAGEAVAPSLEKIKVDGLPTPVLPRIDLAEPQFKYAARAGGLYQSIIPAAL
EHHHHHHHHHCCHHHCCCHHEEEECCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHH
LQQPYAIKAWVMSRSNPMQTVTDRTQVQNALEAMDFVVACDVYISESAAYADVVLPESTY
HCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEECCCCHH
LERDEEIGDMSSKNPAYALRQRVTETIGDTRPSWQIFKEMADAMALGHHFPWQDIVDYQQ
HCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
RQTANNPELLPQLRKEGFLSFGIPLLMREPDMVAKFRQHYPNAQPPSERGDYGNQLQFST
HHCCCCHHHHHHHHHCCCHHCCCHHEECCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEC
PSGKIELTSPSLEEIAPGRSVISYRPVTLKQPNELYFIQGKSALHTNAATQNIPLLFDLQ
CCCEEEECCCCHHHHCCCCCEEEECCEEECCCCEEEEEECCCCEECCCCCCCCCEEEEEC
PDNPVWIHPDTAAEFRINDGDSITMTSQVGEEQGTAKLTLGIRKDTVFAHMGFGSKNQEL
CCCCEEECCCCCEEEEECCCCEEEEEHHCCCCCCCEEEEEEEECCEEEEEECCCCCCHHH
TRAFGKGLHCGALLPDATAAITGSNVHTTGVTIRRA
HHHHCCCCCCCCCCCCCCEEEECCCEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7751291; 11677609; 7737516 [H]