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The map label for this gene is psrB [H]

Identifier: 167625182

GI number: 167625182

Start: 3988181

End: 3988759

Strand: Reverse

Name: psrB [H]

Synonym: Shal_3270

Alternate gene names: 167625182

Gene position: 3988759-3988181 (Counterclockwise)

Preceding gene: 167625183

Following gene: 167625181

Centisome position: 76.31

GC content: 48.19

Gene sequence:

>579_bases
ATGAACGAAAAATTTGTACTCATTCATGATGAAAATCGCTGCATAGGCTGCCATGCCTGCACCGCCGCTTGTAACAGCGC
TAACGAGTTGGCCGATCATCAAAATCGGGCCTCAATTGATGTAAAGGGCCCATATCTTATCGATGGTGAACTGCATTACC
GTTATCTGCGAGTCTCTTGCGAACAGTGTGACAGACCCGCCTGTATCAGGGCCTGTCCTTATGGAGCCATCAGTCAAACA
ATAGATGGTGTCGTGTCATTTAATCATGACCGCTGCAAAGGCTGTTTAATGTGTGTAGCCGCCTGCCCGACAGGGGGATG
TTTCTATAATAAGCAAGTCAAAGCCACGGATAACTGCGATTTTTGCATGAAAAGCCGTGTTGCAGAGGGACTACAGCCAG
CCTGTGTTACGGTATGCCCAGCCGATGCGTTAATTTTTGGCAATGCCAACGATCCAAACAGTGAAGTACATAAGCACCTG
TCGACGGTACTGACTTATAACCGTTCCATTGGCTACGACAATAAACCTAAGCTCTACCGAGTCGCCAGTCAAGTAGCAAG
TGAACGCAGCCGGGTATAG

Upstream 100 bases:

>100_bases
CGATCAGACGCGCGTAATGTGTATTGAGTTTAACTAACAAGGAGCGACTTTACTCCAGCCAATGCAATAGCAAGTGCTGG
AGTAGAGTGGGAGAAACGCA

Downstream 100 bases:

>100_bases
GTTAGACTGCGTCTAACAATTAGGGTCGTGAGCTTCCAGCTCACACTCGGCCAAGAGGGGGTCAACAGCAACCCCCTCTT
GCCTAATTCTTTCACAAGAG

Product: 4Fe-4S ferredoxin iron-sulfur-binding domain-containing protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Sulfur reductase chain B [H]

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MNEKFVLIHDENRCIGCHACTAACNSANELADHQNRASIDVKGPYLIDGELHYRYLRVSCEQCDRPACIRACPYGAISQT
IDGVVSFNHDRCKGCLMCVAACPTGGCFYNKQVKATDNCDFCMKSRVAEGLQPACVTVCPADALIFGNANDPNSEVHKHL
STVLTYNRSIGYDNKPKLYRVASQVASERSRV

Sequences:

>Translated_192_residues
MNEKFVLIHDENRCIGCHACTAACNSANELADHQNRASIDVKGPYLIDGELHYRYLRVSCEQCDRPACIRACPYGAISQT
IDGVVSFNHDRCKGCLMCVAACPTGGCFYNKQVKATDNCDFCMKSRVAEGLQPACVTVCPADALIFGNANDPNSEVHKHL
STVLTYNRSIGYDNKPKLYRVASQVASERSRV
>Mature_192_residues
MNEKFVLIHDENRCIGCHACTAACNSANELADHQNRASIDVKGPYLIDGELHYRYLRVSCEQCDRPACIRACPYGAISQT
IDGVVSFNHDRCKGCLMCVAACPTGGCFYNKQVKATDNCDFCMKSRVAEGLQPACVTVCPADALIFGNANDPNSEVHKHL
STVLTYNRSIGYDNKPKLYRVASQVASERSRV

Specific function: Component of the phosphorylative electron transport system with polysulfide as the terminal acceptor [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=179, Percent_Identity=41.340782122905, Blast_Score=147, Evalue=4e-37,
Organism=Escherichia coli, GI226510944, Length=181, Percent_Identity=37.5690607734807, Blast_Score=129, Evalue=9e-32,
Organism=Escherichia coli, GI1787872, Length=176, Percent_Identity=35.7954545454545, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1787122, Length=176, Percent_Identity=34.0909090909091, Blast_Score=94, Evalue=5e-21,
Organism=Escherichia coli, GI1787749, Length=158, Percent_Identity=33.5443037974684, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1790326, Length=190, Percent_Identity=29.4736842105263, Blast_Score=85, Evalue=2e-18,
Organism=Escherichia coli, GI1789370, Length=180, Percent_Identity=29.4444444444444, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788811, Length=148, Percent_Identity=35.8108108108108, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1787963, Length=151, Percent_Identity=29.1390728476821, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI87082179, Length=145, Percent_Identity=36.551724137931, Blast_Score=67, Evalue=6e-13,
Organism=Escherichia coli, GI1787478, Length=86, Percent_Identity=36.046511627907, Blast_Score=64, Evalue=5e-12,
Organism=Escherichia coli, GI1787740, Length=86, Percent_Identity=33.7209302325581, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 21118; Mature: 21118

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

8.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
10.4 %Cys+Met (Translated Protein)
8.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
10.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEKFVLIHDENRCIGCHACTAACNSANELADHQNRASIDVKGPYLIDGELHYRYLRVSC
CCCCEEEEECCCCEEEHHHHHHHHCCHHHHHHCCCCCEEEECCCEEECCCEEEEEEEEEH
EQCDRPACIRACPYGAISQTIDGVVSFNHDRCKGCLMCVAACPTGGCFYNKQVKATDNCD
HHCCCCCHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEECCEECCCCCCH
FCMKSRVAEGLQPACVTVCPADALIFGNANDPNSEVHKHLSTVLTYNRSIGYDNKPKLYR
HHHHHHHHCCCCCCEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHH
VASQVASERSRV
HHHHHHHHHCCC
>Mature Secondary Structure
MNEKFVLIHDENRCIGCHACTAACNSANELADHQNRASIDVKGPYLIDGELHYRYLRVSC
CCCCEEEEECCCCEEEHHHHHHHHCCHHHHHHCCCCCEEEECCCEEECCCEEEEEEEEEH
EQCDRPACIRACPYGAISQTIDGVVSFNHDRCKGCLMCVAACPTGGCFYNKQVKATDNCD
HHCCCCCHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEECCEECCCCCCH
FCMKSRVAEGLQPACVTVCPADALIFGNANDPNSEVHKHLSTVLTYNRSIGYDNKPKLYR
HHHHHHHHCCCCCCEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHH
VASQVASERSRV
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1597189; 14500908 [H]