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The map label for this gene is hemK [H]

Identifier: 167625122

GI number: 167625122

Start: 3914196

End: 3915038

Strand: Reverse

Name: hemK [H]

Synonym: Shal_3210

Alternate gene names: 167625122

Gene position: 3915038-3914196 (Counterclockwise)

Preceding gene: 167625123

Following gene: 167625121

Centisome position: 74.9

GC content: 46.26

Gene sequence:

>843_bases
TTGCAATTAACTATCTCAGGGGCGCTTAAGTGGGCCTCTTCTCAGCTTGAAGGTATCTCTGACTCAGCATCTCTTGATGC
TGAGGTCATGTTGCTGCACGTGATTAATAAGCCGCGTAGTTATTTATATACTTGGCCTGATAGAAGTTTAGAGCCTGAGC
AGGTGTTTGAGTTTAAACAGATGCTAGCTAAGCGCTTACGTGGTAATCCTATTGCCCATATCGTGGGGGAAAGGGAGTTT
TGGTCACTGCCATTTAGGGTCAATCCGACAACGCTAATTCCACGTCCTGACACTGAAATCTTGGTAGAGACCGCGCTTAA
CTTGCCTTTGGCTGAGAATGCTAAAGTACTCGATTTAGGCACTGGAACTGGAGCGATAGCGCTGTCATTAGCTCATGAGC
GCAATGAGTGGCAAGTGTGCGCGATTGATAAAGTTGAAGATGCGGTCGCCTTGGCAAAAGTTAATCGTGCTAACTTGAAA
CTTGAACAAGTTGATGTGTTTCAAAGTGACTGGTTCGATGCCGTTGAGTGCTACGATTTTAACTTAATCGTGTCTAACCC
ACCTTATATTGATGAGGAAGATGAACACCTTTCTCAAGGTGATGTTAGGTTTGAGCCACAAAGTGCTCTCACTGCACCGC
TGAAAGGGTTTGCCGATCTGTTTCACATTGCAGAGTCTGCTCGTGACTACCTTGCTCCAGGTGGCTATTTGTTACTTGAG
CATGGTTATCAGCAAGCTATCGAGCTTAGAGAGAAGTTAATCGAGCTGGGCTATGAGAATGTTGCCACGGTTCGCGACTT
TGGCAGTAATGATCGCTGCACATTAGGCCGCTTGCCTCGCTAA

Upstream 100 bases:

>100_bases
CGCTTATCCAAGAAAACCAAGCGGATATGCTTGCAGCACTGGGTGAAGCTTAATTCTTCACTAATGTGATTATTGAGCTA
CAGATGAATAAGGATATTCC

Downstream 100 bases:

>100_bases
GTTTTTGGCGTTAGAAAACAAAAAAGGCACGATTGATAGTGCCTTTTTTATTTTAAGCCTAAAGGTAGCCGTTCAGACCC
ATTTCCAATGTTGCCAATTA

Product: HemK family modification methylase

Products: NA

Alternate protein names: M.VchAHemK2P [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MQLTISGALKWASSQLEGISDSASLDAEVMLLHVINKPRSYLYTWPDRSLEPEQVFEFKQMLAKRLRGNPIAHIVGEREF
WSLPFRVNPTTLIPRPDTEILVETALNLPLAENAKVLDLGTGTGAIALSLAHERNEWQVCAIDKVEDAVALAKVNRANLK
LEQVDVFQSDWFDAVECYDFNLIVSNPPYIDEEDEHLSQGDVRFEPQSALTAPLKGFADLFHIAESARDYLAPGGYLLLE
HGYQQAIELREKLIELGYENVATVRDFGSNDRCTLGRLPR

Sequences:

>Translated_280_residues
MQLTISGALKWASSQLEGISDSASLDAEVMLLHVINKPRSYLYTWPDRSLEPEQVFEFKQMLAKRLRGNPIAHIVGEREF
WSLPFRVNPTTLIPRPDTEILVETALNLPLAENAKVLDLGTGTGAIALSLAHERNEWQVCAIDKVEDAVALAKVNRANLK
LEQVDVFQSDWFDAVECYDFNLIVSNPPYIDEEDEHLSQGDVRFEPQSALTAPLKGFADLFHIAESARDYLAPGGYLLLE
HGYQQAIELREKLIELGYENVATVRDFGSNDRCTLGRLPR
>Mature_280_residues
MQLTISGALKWASSQLEGISDSASLDAEVMLLHVINKPRSYLYTWPDRSLEPEQVFEFKQMLAKRLRGNPIAHIVGEREF
WSLPFRVNPTTLIPRPDTEILVETALNLPLAENAKVLDLGTGTGAIALSLAHERNEWQVCAIDKVEDAVALAKVNRANLK
LEQVDVFQSDWFDAVECYDFNLIVSNPPYIDEEDEHLSQGDVRFEPQSALTAPLKGFADLFHIAESARDYLAPGGYLLLE
HGYQQAIELREKLIELGYENVATVRDFGSNDRCTLGRLPR

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=239, Percent_Identity=30.5439330543933, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1787463, Length=269, Percent_Identity=50.9293680297398, Blast_Score=276, Evalue=1e-75,
Organism=Escherichia coli, GI87082085, Length=175, Percent_Identity=32.5714285714286, Blast_Score=82, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24582226, Length=252, Percent_Identity=28.5714285714286, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 31480; Mature: 31480

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLTISGALKWASSQLEGISDSASLDAEVMLLHVINKPRSYLYTWPDRSLEPEQVFEFKQ
CEEEECHHHHHHHHHHCCCCCCCCCCHHEEEEEECCCCCCEEEECCCCCCCHHHHHHHHH
MLAKRLRGNPIAHIVGEREFWSLPFRVNPTTLIPRPDTEILVETALNLPLAENAKVLDLG
HHHHHHCCCCEEEEECCCCEEECCEEECCCEECCCCCHHHHHHHHHCCCCCCCCEEEEEC
TGTGAIALSLAHERNEWQVCAIDKVEDAVALAKVNRANLKLEQVDVFQSDWFDAVECYDF
CCCCEEEEEEECCCCCEEEEEECCHHHHHHHHHHHHHCCEEHHHHHHHHCCCCCEEEEEE
NLIVSNPPYIDEEDEHLSQGDVRFEPQSALTAPLKGFADLFHIAESARDYLAPGGYLLLE
EEEECCCCCCCCCHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
HGYQQAIELREKLIELGYENVATVRDFGSNDRCTLGRLPR
CCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MQLTISGALKWASSQLEGISDSASLDAEVMLLHVINKPRSYLYTWPDRSLEPEQVFEFKQ
CEEEECHHHHHHHHHHCCCCCCCCCCHHEEEEEECCCCCCEEEECCCCCCCHHHHHHHHH
MLAKRLRGNPIAHIVGEREFWSLPFRVNPTTLIPRPDTEILVETALNLPLAENAKVLDLG
HHHHHHCCCCEEEEECCCCEEECCEEECCCEECCCCCHHHHHHHHHCCCCCCCCEEEEEC
TGTGAIALSLAHERNEWQVCAIDKVEDAVALAKVNRANLKLEQVDVFQSDWFDAVECYDF
CCCCEEEEEEECCCCCEEEEEECCHHHHHHHHHHHHHCCEEHHHHHHHHCCCCCEEEEEE
NLIVSNPPYIDEEDEHLSQGDVRFEPQSALTAPLKGFADLFHIAESARDYLAPGGYLLLE
EEEECCCCCCCCCHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
HGYQQAIELREKLIELGYENVATVRDFGSNDRCTLGRLPR
CCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]