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The map label for this gene is pnp

Identifier: 167625054

GI number: 167625054

Start: 3835129

End: 3837243

Strand: Reverse

Name: pnp

Synonym: Shal_3142

Alternate gene names: 167625054

Gene position: 3837243-3835129 (Counterclockwise)

Preceding gene: 167625056

Following gene: 167625053

Centisome position: 73.41

GC content: 45.53

Gene sequence:

>2115_bases
ATGGTTCACGTGAATCCAATCGTAAAGAGTTTTGAATATGGTCAGCACACAGTCACATTAGAGACTGGGGTTATCGCGCG
TCAAGCAGATGCAGCTGTTTTGGCAAGTATGGGTGATACAACAGTATTGGTTACTGTTGTTGGCAAGAAGTTTGAAGAGC
CAGGCCGTGACTTTTTCCCGCTAACCGTTAATTACCAGGAAAAAACATACGCAGCAGGTAAGATCCCAGGTGGATTCTTC
AAGCGCGAAGGTCGTCCATCTGAAGGTGAAACGCTAACAGCACGCCTAATTGACCGTCCAATCCGTCCACTATTCCCGAA
TGGTTTTAAGAACGAAGTACAGGTTATCATTACTGTTGTTTCTGTAGACCCAGAAATCAGCCCAGAAGTTATCTCTATGA
TTGGTACTTCTGCTGCACTGTCTATTTCTGGTATTCCATTTAACGGTCCACTAGGCTCGGCACGTGTCGGTTATGTGAAC
GGCGAATACATCCTTAACCCAACAGTTTCTCAACTAGTTGATAGCCAGCTTGAGCTTTCTGTTGCCGGTACTGACAGTGC
AGTACTAATGGTTGAATCTGAAGCAAGCGCATTGCCTGAAGAAGTAATGTTAGGCGCGGTTGTTTATGGTCACGACCAGC
AGCAAGTTGTTATCCAAGCAATTAAAGAGCTACAAGCTGAAGTTAACAAGCCTGTATGGGATTGGGCTGCACCTGTGCAA
GACGAAACTCTTGTTGCTAAGATCAAAGATTTGGCTGAAGCTGGTCTAACTGAAGCTTACCAAATTGAAGTTAAGCAAGA
TCGTTACGCTCAAGTTGGTGTAGTTAAGAATGCTGCTAAAGAAGCATTGTTAGCTGAAAACCCAGATGCTAATACACGTG
AAATCGACGGTCTTCTAGGTAGCCTAGAGAAGAAAGTGGTTCGTGGCCGTATTATCGCGGGTAACCCACGTATCGATGGT
CGTGAGCCAGATATGGTTCGTGCACTTAACGTTATGGCTGGTGTACTTCCACGTACTCACGGTAGCTCACTGTTCACTCG
TGGTGAAACTCAGGCGCTAGTAACTTGTACTCTTGGTACAGAGCGTGATGCACAGAAGATTGATAGCATCATGGGCGAGT
ACACTAACCGCTTTATGCTGCACTACAACTTCCCTCCATACTCTGTTGGTGAAACTGGCATGGTAGGTTCTCCTAAGCGT
CGTGAAATTGGTCACGGTAAGCTAGCATGGCGTGGTATTAACGCGGTTATGCCTACTGCTGAAGAGTTCCCATACAGCGT
TCGTATCGTATCTGAAATCACTGAATCTAACGGTTCAAGCTCTATGGCGTCAGTTTGTGGTACTTCTCTTGCTCTTATGG
ATGCAGGTGTACCAATCAAGACTTCAGTTGCGGGTATTGCGATGGGTCTAGTTAAAGAAGGCGACGATTTCGTAGTTCTT
TCTGACATTCTAGGCGATGAAGATCATCTTGGTGATATGGACTTTAAAGTTGCGGGTACTCGCGATGGTATTACTGCTCT
GCAGATGGATATCAAGATCGAAGGTATCACTAAAGAGATCATGCAGATTGCTCTACAGCAAGCATACGGCGCACGTGTTC
ATATTCTAAACGTGATGGACCAAGCGATTGGTTCACATCGTGGTGATATCTCTGAGCACGCGCCACGTATCACGACCATC
AAGATCAACCCTGAAAAGATCCGTGATGTTATTGGTAAAGGTGGTGCAACGATTCGTGCACTTACTGAAGAAACCGGTAC
TACGATTGAACTTGATGACGACGGTACAGTGAAGATTGCATCTTCAAACGGCGAAGCGACTAAAGAAGCTATCCGTCGTA
TCGAAGAGATCACCGCTGAAGTTGAAGTGGGTACAGTTTACAACGGTAAAGTTGTTCGTATCGTAGATTTTGGTGCTTTC
GTGACGATTCTTCCAGGTAAAGATGGTCTAGTACATATTTCTCAAATCGCTGAAGAGCGCGTTGCGAACGTGTCTGACTA
CCTAGAAGTAGGTCAAGAAGTTAAAGTTAAAGTGATGGAAGTTGACCGTCAAGGCCGTGTACGTCTTTCTATGAAAGAAG
CTGCACCTAAGGCTGATGCGCCAGCTGCTGAATAA

Upstream 100 bases:

>100_bases
AGAGAAAAGATTGTGCTTTGTCATCCATCTATTCTCTTTATCTATTGAATCAACTTGCAGTATACTTACGCGCGTAATTA
AGGTAATTAAATATTAAGGA

Downstream 100 bases:

>100_bases
GCACTCGCACTTTAAATGAATAGGAGGCCTTGAGCCTCCTTTTTTTATGCGAAAAATTAAAGTTGACCAATTAATCTGTG
TTAATTTGATTATTTTCAAT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 704; Mature: 704

Protein sequence:

>704_residues
MVHVNPIVKSFEYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKFEEPGRDFFPLTVNYQEKTYAAGKIPGGFF
KREGRPSEGETLTARLIDRPIRPLFPNGFKNEVQVIITVVSVDPEISPEVISMIGTSAALSISGIPFNGPLGSARVGYVN
GEYILNPTVSQLVDSQLELSVAGTDSAVLMVESEASALPEEVMLGAVVYGHDQQQVVIQAIKELQAEVNKPVWDWAAPVQ
DETLVAKIKDLAEAGLTEAYQIEVKQDRYAQVGVVKNAAKEALLAENPDANTREIDGLLGSLEKKVVRGRIIAGNPRIDG
REPDMVRALNVMAGVLPRTHGSSLFTRGETQALVTCTLGTERDAQKIDSIMGEYTNRFMLHYNFPPYSVGETGMVGSPKR
REIGHGKLAWRGINAVMPTAEEFPYSVRIVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDDFVVL
SDILGDEDHLGDMDFKVAGTRDGITALQMDIKIEGITKEIMQIALQQAYGARVHILNVMDQAIGSHRGDISEHAPRITTI
KINPEKIRDVIGKGGATIRALTEETGTTIELDDDGTVKIASSNGEATKEAIRRIEEITAEVEVGTVYNGKVVRIVDFGAF
VTILPGKDGLVHISQIAEERVANVSDYLEVGQEVKVKVMEVDRQGRVRLSMKEAAPKADAPAAE

Sequences:

>Translated_704_residues
MVHVNPIVKSFEYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKFEEPGRDFFPLTVNYQEKTYAAGKIPGGFF
KREGRPSEGETLTARLIDRPIRPLFPNGFKNEVQVIITVVSVDPEISPEVISMIGTSAALSISGIPFNGPLGSARVGYVN
GEYILNPTVSQLVDSQLELSVAGTDSAVLMVESEASALPEEVMLGAVVYGHDQQQVVIQAIKELQAEVNKPVWDWAAPVQ
DETLVAKIKDLAEAGLTEAYQIEVKQDRYAQVGVVKNAAKEALLAENPDANTREIDGLLGSLEKKVVRGRIIAGNPRIDG
REPDMVRALNVMAGVLPRTHGSSLFTRGETQALVTCTLGTERDAQKIDSIMGEYTNRFMLHYNFPPYSVGETGMVGSPKR
REIGHGKLAWRGINAVMPTAEEFPYSVRIVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDDFVVL
SDILGDEDHLGDMDFKVAGTRDGITALQMDIKIEGITKEIMQIALQQAYGARVHILNVMDQAIGSHRGDISEHAPRITTI
KINPEKIRDVIGKGGATIRALTEETGTTIELDDDGTVKIASSNGEATKEAIRRIEEITAEVEVGTVYNGKVVRIVDFGAF
VTILPGKDGLVHISQIAEERVANVSDYLEVGQEVKVKVMEVDRQGRVRLSMKEAAPKADAPAAE
>Mature_704_residues
MVHVNPIVKSFEYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKFEEPGRDFFPLTVNYQEKTYAAGKIPGGFF
KREGRPSEGETLTARLIDRPIRPLFPNGFKNEVQVIITVVSVDPEISPEVISMIGTSAALSISGIPFNGPLGSARVGYVN
GEYILNPTVSQLVDSQLELSVAGTDSAVLMVESEASALPEEVMLGAVVYGHDQQQVVIQAIKELQAEVNKPVWDWAAPVQ
DETLVAKIKDLAEAGLTEAYQIEVKQDRYAQVGVVKNAAKEALLAENPDANTREIDGLLGSLEKKVVRGRIIAGNPRIDG
REPDMVRALNVMAGVLPRTHGSSLFTRGETQALVTCTLGTERDAQKIDSIMGEYTNRFMLHYNFPPYSVGETGMVGSPKR
REIGHGKLAWRGINAVMPTAEEFPYSVRIVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDDFVVL
SDILGDEDHLGDMDFKVAGTRDGITALQMDIKIEGITKEIMQIALQQAYGARVHILNVMDQAIGSHRGDISEHAPRITTI
KINPEKIRDVIGKGGATIRALTEETGTTIELDDDGTVKIASSNGEATKEAIRRIEEITAEVEVGTVYNGKVVRIVDFGAF
VTILPGKDGLVHISQIAEERVANVSDYLEVGQEVKVKVMEVDRQGRVRLSMKEAAPKADAPAAE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=709, Percent_Identity=37.9407616361072, Blast_Score=434, Evalue=1e-121,
Organism=Escherichia coli, GI145693187, Length=695, Percent_Identity=72.3741007194245, Blast_Score=1041, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=709, Percent_Identity=32.5811001410437, Blast_Score=340, Evalue=1e-93,
Organism=Caenorhabditis elegans, GI17535281, Length=75, Percent_Identity=49.3333333333333, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI281362905, Length=709, Percent_Identity=38.2228490832158, Blast_Score=457, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24651641, Length=709, Percent_Identity=38.2228490832158, Blast_Score=457, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24651643, Length=709, Percent_Identity=38.2228490832158, Blast_Score=457, Evalue=1e-128,
Organism=Drosophila melanogaster, GI161079377, Length=655, Percent_Identity=37.8625954198473, Blast_Score=417, Evalue=1e-116,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_SHEHH (B0TQ97)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001675348.1
- ProteinModelPortal:   B0TQ97
- GeneID:   5906120
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_3142
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- BioCyc:   SHAL458817:SHAL_3142-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF54814 KH_prok; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 75842; Mature: 75842

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVHVNPIVKSFEYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKFEEPGRDFFP
CEECCHHHHHHCCCCEEEEEECCCEEECCCHHHHHCCCCCEEEEEEECCHHCCCCCCEEE
LTVNYQEKTYAAGKIPGGFFKREGRPSEGETLTARLIDRPIRPLFPNGFKNEVQVIITVV
EEEECCCCEEECCCCCCCHHCCCCCCCCCCEEEHHHHCCCCCCCCCCCCCCCEEEEEEEE
SVDPEISPEVISMIGTSAALSISGIPFNGPLGSARVGYVNGEYILNPTVSQLVDSQLELS
ECCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCEEECCCHHHHCCCCEEEE
VAGTDSAVLMVESEASALPEEVMLGAVVYGHDQQQVVIQAIKELQAEVNKPVWDWAAPVQ
EECCCCEEEEEECCHHHCCHHHHEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DETLVAKIKDLAEAGLTEAYQIEVKQDRYAQVGVVKNAAKEALLAENPDANTREIDGLLG
CCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
SLEKKVVRGRIIAGNPRIDGREPDMVRALNVMAGVLPRTHGSSLFTRGETQALVTCTLGT
HHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCEEEEEEECCC
ERDAQKIDSIMGEYTNRFMLHYNFPPYSVGETGMVGSPKRREIGHGKLAWRGINAVMPTA
CCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCH
EEFPYSVRIVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDDFVVL
HHCCEEEEEEHHHHCCCCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEE
SDILGDEDHLGDMDFKVAGTRDGITALQMDIKIEGITKEIMQIALQQAYGARVHILNVMD
HHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHHCCCEEEEHHHHH
QAIGSHRGDISEHAPRITTIKINPEKIRDVIGKGGATIRALTEETGTTIELDDDGTVKIA
HHHCCCCCCHHHCCCEEEEEEECHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCEEEEE
SSNGEATKEAIRRIEEITAEVEVGTVYNGKVVRIVDFGAFVTILPGKDGLVHISQIAEER
CCCCHHHHHHHHHHHHHHHEEEEEEEECCEEEEEEECCEEEEEECCCCCCEEHHHHHHHH
VANVSDYLEVGQEVKVKVMEVDRQGRVRLSMKEAAPKADAPAAE
HHCHHHHHHCCCCEEEEEEEECCCCCEEEEHHHCCCCCCCCCCC
>Mature Secondary Structure
MVHVNPIVKSFEYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKFEEPGRDFFP
CEECCHHHHHHCCCCEEEEEECCCEEECCCHHHHHCCCCCEEEEEEECCHHCCCCCCEEE
LTVNYQEKTYAAGKIPGGFFKREGRPSEGETLTARLIDRPIRPLFPNGFKNEVQVIITVV
EEEECCCCEEECCCCCCCHHCCCCCCCCCCEEEHHHHCCCCCCCCCCCCCCCEEEEEEEE
SVDPEISPEVISMIGTSAALSISGIPFNGPLGSARVGYVNGEYILNPTVSQLVDSQLELS
ECCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCEEECCCHHHHCCCCEEEE
VAGTDSAVLMVESEASALPEEVMLGAVVYGHDQQQVVIQAIKELQAEVNKPVWDWAAPVQ
EECCCCEEEEEECCHHHCCHHHHEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DETLVAKIKDLAEAGLTEAYQIEVKQDRYAQVGVVKNAAKEALLAENPDANTREIDGLLG
CCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
SLEKKVVRGRIIAGNPRIDGREPDMVRALNVMAGVLPRTHGSSLFTRGETQALVTCTLGT
HHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCEEEEEEECCC
ERDAQKIDSIMGEYTNRFMLHYNFPPYSVGETGMVGSPKRREIGHGKLAWRGINAVMPTA
CCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCH
EEFPYSVRIVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDDFVVL
HHCCEEEEEEHHHHCCCCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEE
SDILGDEDHLGDMDFKVAGTRDGITALQMDIKIEGITKEIMQIALQQAYGARVHILNVMD
HHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHHCCCEEEEHHHHH
QAIGSHRGDISEHAPRITTIKINPEKIRDVIGKGGATIRALTEETGTTIELDDDGTVKIA
HHHCCCCCCHHHCCCEEEEEEECHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCEEEEE
SSNGEATKEAIRRIEEITAEVEVGTVYNGKVVRIVDFGAFVTILPGKDGLVHISQIAEER
CCCCHHHHHHHHHHHHHHHEEEEEEEECCEEEEEEECCEEEEEECCCCCCEEHHHHHHHH
VANVSDYLEVGQEVKVKVMEVDRQGRVRLSMKEAAPKADAPAAE
HHCHHHHHHCCCCEEEEEEEECCCCCEEEEHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA