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The map label for this gene is prfC

Identifier: 167625052

GI number: 167625052

Start: 3832323

End: 3833903

Strand: Reverse

Name: prfC

Synonym: Shal_3140

Alternate gene names: 167625052

Gene position: 3833903-3832323 (Counterclockwise)

Preceding gene: 167625053

Following gene: 167625051

Centisome position: 73.35

GC content: 45.67

Gene sequence:

>1581_bases
ATGTCAGGCAATAAAGTTGAGGTCGACAAACGTCGCACTTTCGCCATCATCTCTCACCCAGATGCGGGTAAAACCACCAT
CACTGAAAAGGTTCTTTTATTCGGAAACGCGCTACAGAAAGCGGGTACCGTAAAGGGCAAGAAATCAGGTCAGCACGCTA
AGTCTGACTGGATGGAGATGGAAAAGGATCGCGGGATCTCAATTACGACTTCTGTAATGCAGTTTCCTTATCATGATGCG
CTCGTCAACCTGTTAGATACGCCTGGTCACGAAGATTTCTCTGAAGACACTTATCGTACCTTAACGGCGGTTGACTCATG
CTTGATGGTTATCGACTCTGCGAAAGGTGTTGAGCAACGTACTATTAAGCTAATGGAAGTGACACGTCTGCGTGATACGC
CTATTGTGACTTTCATGAACAAACTTGACCGTGATATTCGAGACCCAATCGAATTGATGGACGAAGTCGAGGAAGTGCTT
AATATTAAGTGTGCACCGATCACTTGGCCAATTGGTTGTGGTAAAGAGTTCAAAGGTGTTTACCACTTGCTGCGTGATGA
AGTGATCCTGTACCAAGGCGGTATGGGACATACCATTCAAGACTCTCGGGTCATCAAAGGGCTAGATAATCCTGAGCTTG
ATGACGCGATTGGTAGCTACGCTGCCGATATTCGTGATGAGATGGAGTTGGTTGTTGGTGCGTCACACGAATTTGATCTC
GAGTTGTTCTTGAAAGGCGAGTTAACACCGGTCTACTTCGGTACCGCGTTAGGTAACTTTGGTGTGGACCATATTCTTGA
CGGTATCGTAGAGTGGGCACCAATACCACAACCACGCGAAACCGAAACGCGTGATGTGCAGCCTGAAGAAGAGAAGTTCA
GTGGTTTCGTGTTTAAGATCCAAGCAAACATGGATCCTAAGCATCGTGACCGTGTCGCCTTTATGCGAGTTTGTTCTGGT
CGTTATGAGCAAGGTATGAAGATGCATCACGTTCGTTTAGGTAAAGACGTTAACGTAAGTGATGCGTTGACCTTTATGGC
GGGGGATCGTAACCGTGCTGAAGCCGCATATCCTGGTGATATTATCGGTTTGCATAACCATGGCACGATTCGAATCGGTG
ATACCTTTACTCAAGGTGAGAAGCTTCGCTTTACTGGTGTGCCTAACTTTGCACCTGAGATGTTCCGCCGTATTCGTCTA
AGAGATCCGTTAAAGCAAAAGCAACTGCTTAAAGGGTTGGTACAGCTCTCGGAAGAGGGCGCTGTACAGGTATTTAGACC
ACTAGACAGTAATGATTTAATTGTGGGCGCTGTCGGTGTGCTGCAGTTTGAAGTTGTTGTTGGTCGTTTAAAGACAGAAT
ATAAAGTAGAAGCGATTTACGAGGCTATTAGTGTTGCCACCGCACGCTGGGTATATTGTAACGATCACAAGAAGCTTGAT
GAATTTAAGCGTAAGTGCAGCATGAACTTAGCATTGGATGGTGGTGATAACTTGACTTATATTGCACCGACAATGGTTAA
CCTTAACCTGTCGATGGAACGTTATCCTGATATTGAATTTGCTAAGACTCGCGAAAACTAA

Upstream 100 bases:

>100_bases
AAAGTGAGGCATAGCGATTTAACAGCCTAGTTTGGTAGCAAAAACTAGGGTGAACGGCTATAATTGCGGCCTTTGTGAAC
TCCCAGAATTACAGGTAAAC

Downstream 100 bases:

>100_bases
TACGCGGTTAACTATTCAATACTAAAAACCCCAGGCTCTGCTTGGGGTTTTGCTTTTTATATCGCTGAGTTTTCATCTTT
TCAATTATATTGGTAACCCA

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSGNKVEVDKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWMEMEKDRGISITTSVMQFPYHDA
LVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEQRTIKLMEVTRLRDTPIVTFMNKLDRDIRDPIELMDEVEEVL
NIKCAPITWPIGCGKEFKGVYHLLRDEVILYQGGMGHTIQDSRVIKGLDNPELDDAIGSYAADIRDEMELVVGASHEFDL
ELFLKGELTPVYFGTALGNFGVDHILDGIVEWAPIPQPRETETRDVQPEEEKFSGFVFKIQANMDPKHRDRVAFMRVCSG
RYEQGMKMHHVRLGKDVNVSDALTFMAGDRNRAEAAYPGDIIGLHNHGTIRIGDTFTQGEKLRFTGVPNFAPEMFRRIRL
RDPLKQKQLLKGLVQLSEEGAVQVFRPLDSNDLIVGAVGVLQFEVVVGRLKTEYKVEAIYEAISVATARWVYCNDHKKLD
EFKRKCSMNLALDGGDNLTYIAPTMVNLNLSMERYPDIEFAKTREN

Sequences:

>Translated_526_residues
MSGNKVEVDKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWMEMEKDRGISITTSVMQFPYHDA
LVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEQRTIKLMEVTRLRDTPIVTFMNKLDRDIRDPIELMDEVEEVL
NIKCAPITWPIGCGKEFKGVYHLLRDEVILYQGGMGHTIQDSRVIKGLDNPELDDAIGSYAADIRDEMELVVGASHEFDL
ELFLKGELTPVYFGTALGNFGVDHILDGIVEWAPIPQPRETETRDVQPEEEKFSGFVFKIQANMDPKHRDRVAFMRVCSG
RYEQGMKMHHVRLGKDVNVSDALTFMAGDRNRAEAAYPGDIIGLHNHGTIRIGDTFTQGEKLRFTGVPNFAPEMFRRIRL
RDPLKQKQLLKGLVQLSEEGAVQVFRPLDSNDLIVGAVGVLQFEVVVGRLKTEYKVEAIYEAISVATARWVYCNDHKKLD
EFKRKCSMNLALDGGDNLTYIAPTMVNLNLSMERYPDIEFAKTREN
>Mature_525_residues
SGNKVEVDKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWMEMEKDRGISITTSVMQFPYHDAL
VNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEQRTIKLMEVTRLRDTPIVTFMNKLDRDIRDPIELMDEVEEVLN
IKCAPITWPIGCGKEFKGVYHLLRDEVILYQGGMGHTIQDSRVIKGLDNPELDDAIGSYAADIRDEMELVVGASHEFDLE
LFLKGELTPVYFGTALGNFGVDHILDGIVEWAPIPQPRETETRDVQPEEEKFSGFVFKIQANMDPKHRDRVAFMRVCSGR
YEQGMKMHHVRLGKDVNVSDALTFMAGDRNRAEAAYPGDIIGLHNHGTIRIGDTFTQGEKLRFTGVPNFAPEMFRRIRLR
DPLKQKQLLKGLVQLSEEGAVQVFRPLDSNDLIVGAVGVLQFEVVVGRLKTEYKVEAIYEAISVATARWVYCNDHKKLDE
FKRKCSMNLALDGGDNLTYIAPTMVNLNLSMERYPDIEFAKTREN

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily

Homologues:

Organism=Homo sapiens, GI18390331, Length=498, Percent_Identity=26.5060240963855, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI19923640, Length=528, Percent_Identity=26.7045454545455, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI25306283, Length=394, Percent_Identity=27.9187817258883, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI25306287, Length=500, Percent_Identity=25.8, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI157426893, Length=180, Percent_Identity=27.7777777777778, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI94966754, Length=139, Percent_Identity=30.9352517985612, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=28.1879194630872, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1790835, Length=521, Percent_Identity=78.3109404990403, Blast_Score=867, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=498, Percent_Identity=25.7028112449799, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=30.1282051282051, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI48994988, Length=198, Percent_Identity=29.7979797979798, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17533571, Length=482, Percent_Identity=27.1784232365145, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17556745, Length=230, Percent_Identity=26.9565217391304, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17557151, Length=135, Percent_Identity=31.1111111111111, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17506493, Length=178, Percent_Identity=28.6516853932584, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=479, Percent_Identity=26.9311064718163, Blast_Score=165, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6322359, Length=530, Percent_Identity=23.2075471698113, Blast_Score=113, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6323320, Length=158, Percent_Identity=23.4177215189873, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6324707, Length=156, Percent_Identity=30.1282051282051, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320593, Length=156, Percent_Identity=30.1282051282051, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24582462, Length=475, Percent_Identity=27.1578947368421, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI221458488, Length=392, Percent_Identity=26.7857142857143, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI78706572, Length=159, Percent_Identity=30.8176100628931, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24585711, Length=169, Percent_Identity=30.1775147928994, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24585713, Length=169, Percent_Identity=30.1775147928994, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24585709, Length=169, Percent_Identity=30.1775147928994, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF3_SHEHH (B0TQ95)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001675346.1
- ProteinModelPortal:   B0TQ95
- SMR:   B0TQ95
- GeneID:   5906479
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_3140
- HOGENOM:   HBG285678
- OMA:   LQFEVVQ
- ProtClustDB:   PRK00741
- BioCyc:   SHAL458817:SHAL_3140-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00072
- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00503
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 59321; Mature: 59189

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGNKVEVDKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWMEM
CCCCEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
EKDRGISITTSVMQFPYHDALVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEQR
HHCCCCEEEHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHH
TIKLMEVTRLRDTPIVTFMNKLDRDIRDPIELMDEVEEVLNIKCAPITWPIGCGKEFKGV
HHHHHHHHHHCCCHHHHHHHHHCHHHCCHHHHHHHHHHHHCCEECCEEECCCCCHHHHHH
YHLLRDEVILYQGGMGHTIQDSRVIKGLDNPELDDAIGSYAADIRDEMELVVGASHEFDL
HHHHHCCEEEEECCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCE
ELFLKGELTPVYFGTALGNFGVDHILDGIVEWAPIPQPRETETRDVQPEEEKFSGFVFKI
EEEEECCCCEEEEEHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCEEEEE
QANMDPKHRDRVAFMRVCSGRYEQGMKMHHVRLGKDVNVSDALTFMAGDRNRAEAAYPGD
ECCCCCCHHHHHHHHHHHCCHHHCCCEEEEEECCCCCCHHHHEEEEECCCCCCCCCCCCC
IIGLHNHGTIRIGDTFTQGEKLRFTGVPNFAPEMFRRIRLRDPLKQKQLLKGLVQLSEEG
EEEECCCCEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
AVQVFRPLDSNDLIVGAVGVLQFEVVVGRLKTEYKVEAIYEAISVATARWVYCNDHKKLD
CEEEEECCCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEEECCCHHHH
EFKRKCSMNLALDGGDNLTYIAPTMVNLNLSMERYPDIEFAKTREN
HHHHHHCCEEEEECCCCEEEEEEEEEEEEECHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SGNKVEVDKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWMEM
CCCEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
EKDRGISITTSVMQFPYHDALVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEQR
HHCCCCEEEHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHH
TIKLMEVTRLRDTPIVTFMNKLDRDIRDPIELMDEVEEVLNIKCAPITWPIGCGKEFKGV
HHHHHHHHHHCCCHHHHHHHHHCHHHCCHHHHHHHHHHHHCCEECCEEECCCCCHHHHHH
YHLLRDEVILYQGGMGHTIQDSRVIKGLDNPELDDAIGSYAADIRDEMELVVGASHEFDL
HHHHHCCEEEEECCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCE
ELFLKGELTPVYFGTALGNFGVDHILDGIVEWAPIPQPRETETRDVQPEEEKFSGFVFKI
EEEEECCCCEEEEEHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCEEEEE
QANMDPKHRDRVAFMRVCSGRYEQGMKMHHVRLGKDVNVSDALTFMAGDRNRAEAAYPGD
ECCCCCCHHHHHHHHHHHCCHHHCCCEEEEEECCCCCCHHHHEEEEECCCCCCCCCCCCC
IIGLHNHGTIRIGDTFTQGEKLRFTGVPNFAPEMFRRIRLRDPLKQKQLLKGLVQLSEEG
EEEECCCCEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
AVQVFRPLDSNDLIVGAVGVLQFEVVVGRLKTEYKVEAIYEAISVATARWVYCNDHKKLD
CEEEEECCCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEEECCCHHHH
EFKRKCSMNLALDGGDNLTYIAPTMVNLNLSMERYPDIEFAKTREN
HHHHHHCCEEEEECCCCEEEEEEEEEEEEECHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA