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The map label for this gene is leuO [H]

Identifier: 167624917

GI number: 167624917

Start: 3645584

End: 3646486

Strand: Reverse

Name: leuO [H]

Synonym: Shal_3003

Alternate gene names: 167624917

Gene position: 3646486-3645584 (Counterclockwise)

Preceding gene: 167624919

Following gene: 167624916

Centisome position: 69.76

GC content: 44.19

Gene sequence:

>903_bases
ATGTCACAGCAAAAGTCATTCGATCTTAATTTATTTCGCATTTTTACTGCGGTGTACCGTACGTCATCATTTAGCCGCGC
AGCCGAGGAGTTAGACCTGACTCAATCGTCGGTGAGTAATGCCATTGCGCGTCTTAAGTTATCGGTGGGGGAAGAGCTGT
TTATTCGCGTTGGTAGAGGAGTGAAACCTACGGCGAGTGCGATGAACTTATATCAGCAGCTTGCGAGTCCTGTGTCTGAA
ATCGAACAGGTCGCTTCAAGTTTTGAGGTATTTGAAGCCAGTACCAGTGAACGTACCTTCTATGTCTATGCCAGTGAGTC
AGCAATTCAGCTACTGCAAGGCTTAGTTGAGCAAGCGTTAGAAGGGCTCTCAATCAATATTGTATTTCGTGAGCCGCCAT
TAATTGAGGAAGAGTTGCAGATGGAGTTGCAGCTTGAGAAAGTCGACCTAGCACTGGATATTTGGTTACCTGAATCAGCA
TCTTTTAAGAGCCAAAAGGTGATGCAAGATAAGTTGGTGTGTGTGGCCAGAAAAGGCCACCCGCGCATCACAGGGGCGAT
TACCCAAGAGCAGTATTTTAGTGAAAAGCATATTATTTTTAAGATGCGCCGCTATAACCTTAGCGTTGCAGATCTACTGA
CTAAACATGTTCTACCGGCGAGACAGATGCACAGTGAGCAATCATCGATTTTAAGTATGATGGCCGTGGCCTCTAAAAGT
GATGCCTTAGCTATCGCCTCTCAATCCTATACCGATGAATATGCAGAATTGTTTGGCCTACAGGTATTTGAGTTGCCCTT
TGATGTTGAGCCTATCGAATATTACATGGTATGGAGCCATAAGCTCGAGCAAAACCCAGCCAATATTTGGTTGAGAAATA
CGATACTAAAGTTAATTAAATAA

Upstream 100 bases:

>100_bases
TAGCGAGGGAAATTCGAATAATAATTAGGGTGAACTTTACTTTTAATATTTATAATATGAATATTACTGCAGCCATTATT
AACGCTAAGCGGAAATATTT

Downstream 100 bases:

>100_bases
GTAGGTTAATCCGAGTTTAGATAGTGTGTTAAAGTGAATTATTTGCTTAAATATGGTTCAACGGTTAAGCGCTAAGATAG
AGGGAAAGCAATGACACAAC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MSQQKSFDLNLFRIFTAVYRTSSFSRAAEELDLTQSSVSNAIARLKLSVGEELFIRVGRGVKPTASAMNLYQQLASPVSE
IEQVASSFEVFEASTSERTFYVYASESAIQLLQGLVEQALEGLSINIVFREPPLIEEELQMELQLEKVDLALDIWLPESA
SFKSQKVMQDKLVCVARKGHPRITGAITQEQYFSEKHIIFKMRRYNLSVADLLTKHVLPARQMHSEQSSILSMMAVASKS
DALAIASQSYTDEYAELFGLQVFELPFDVEPIEYYMVWSHKLEQNPANIWLRNTILKLIK

Sequences:

>Translated_300_residues
MSQQKSFDLNLFRIFTAVYRTSSFSRAAEELDLTQSSVSNAIARLKLSVGEELFIRVGRGVKPTASAMNLYQQLASPVSE
IEQVASSFEVFEASTSERTFYVYASESAIQLLQGLVEQALEGLSINIVFREPPLIEEELQMELQLEKVDLALDIWLPESA
SFKSQKVMQDKLVCVARKGHPRITGAITQEQYFSEKHIIFKMRRYNLSVADLLTKHVLPARQMHSEQSSILSMMAVASKS
DALAIASQSYTDEYAELFGLQVFELPFDVEPIEYYMVWSHKLEQNPANIWLRNTILKLIK
>Mature_299_residues
SQQKSFDLNLFRIFTAVYRTSSFSRAAEELDLTQSSVSNAIARLKLSVGEELFIRVGRGVKPTASAMNLYQQLASPVSEI
EQVASSFEVFEASTSERTFYVYASESAIQLLQGLVEQALEGLSINIVFREPPLIEEELQMELQLEKVDLALDIWLPESAS
FKSQKVMQDKLVCVARKGHPRITGAITQEQYFSEKHIIFKMRRYNLSVADLLTKHVLPARQMHSEQSSILSMMAVASKSD
ALAIASQSYTDEYAELFGLQVFELPFDVEPIEYYMVWSHKLEQNPANIWLRNTILKLIK

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081684, Length=304, Percent_Identity=27.3026315789474, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI87081766, Length=263, Percent_Identity=26.615969581749, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1786820, Length=283, Percent_Identity=25.7950530035336, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789204, Length=256, Percent_Identity=25.78125, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 825 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34076; Mature: 33944

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQKSFDLNLFRIFTAVYRTSSFSRAAEELDLTQSSVSNAIARLKLSVGEELFIRVGRG
CCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCC
VKPTASAMNLYQQLASPVSEIEQVASSFEVFEASTSERTFYVYASESAIQLLQGLVEQAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHHHHHHH
EGLSINIVFREPPLIEEELQMELQLEKVDLALDIWLPESASFKSQKVMQDKLVCVARKGH
CCCEEEEEECCCCCCHHHHHHHHEEEEEEEEEEEECCCCCCCHHHHHHHHHHHEEECCCC
PRITGAITQEQYFSEKHIIFKMRRYNLSVADLLTKHVLPARQMHSEQSSILSMMAVASKS
CCEEEEECHHHHHCCCHHEEEHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
DALAIASQSYTDEYAELFGLQVFELPFDVEPIEYYMVWSHKLEQNPANIWLRNTILKLIK
CCEEEECCHHHHHHHHHHCCEEEECCCCCCCEEEEEEEHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SQQKSFDLNLFRIFTAVYRTSSFSRAAEELDLTQSSVSNAIARLKLSVGEELFIRVGRG
CCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCC
VKPTASAMNLYQQLASPVSEIEQVASSFEVFEASTSERTFYVYASESAIQLLQGLVEQAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHHHHHHH
EGLSINIVFREPPLIEEELQMELQLEKVDLALDIWLPESASFKSQKVMQDKLVCVARKGH
CCCEEEEEECCCCCCHHHHHHHHEEEEEEEEEEEECCCCCCCHHHHHHHHHHHEEECCCC
PRITGAITQEQYFSEKHIIFKMRRYNLSVADLLTKHVLPARQMHSEQSSILSMMAVASKS
CCEEEEECHHHHHCCCHHEEEHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
DALAIASQSYTDEYAELFGLQVFELPFDVEPIEYYMVWSHKLEQNPANIWLRNTILKLIK
CCEEEECCHHHHHHHHHHCCEEEECCCCCCCEEEEEEEHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 3519576 [H]