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The map label for this gene is tilS [H]

Identifier: 167624879

GI number: 167624879

Start: 3606036

End: 3607466

Strand: Reverse

Name: tilS [H]

Synonym: Shal_2965

Alternate gene names: 167624879

Gene position: 3607466-3606036 (Counterclockwise)

Preceding gene: 167624880

Following gene: 167624877

Centisome position: 69.02

GC content: 45.63

Gene sequence:

>1431_bases
ATGAATATTGTCACGCTGATCCAAGCTTGCATACAGCAGCTCCCATCGAAAGTGGCGGCTGCAGTTAAGGTCGATAACTC
TCAAGAGCAAAATCAAGCTGCCATAAATAAGCCTAAGCCTAAATTAGTGTTAGCTTATAGTGGCGGCGTTGACTCTGAAA
TTCTTGCCCATGGTTTAGCCCAGTTTGCCAAAGTCTCTCCCCATTTTGAGTGTTTGTTAGTACATGTACACCATGGTTTA
AGTGCTAATGCAGACACTTGGGCTGAACACTGCCTAACTCAAGCGAAGCACTATGATCTTGACTGCACAGTAAAGCGTGT
CGAGGTCAAACTGGCTCCTCGGCTGAGTGTTGAAGCACAGGCCCGCAGCGTGCGTTATGAAGCATTATTATCAGAGTTAT
CTAACAATGATATTTTAATGACGGCGCACCATCAGGATGATCAACTTGAGACTGTTTTATTAGCACTAAAGCGGGGCCTT
GGGCCTAAAGGTTTGTCAGCCATGGGCAAAGTGCAGCGCTTTAATCAGCAACACTGGATTATTAGACCGCTGCTCGATTG
CAGTAAAGATGAGATTGAAGCTTATGCCAAACGATATGAAATCACTCATATCCAAGATGAAAGTAATTTTGATGATAAAT
ACGATAGAAACTTTCTTCGCTTAGATGTTATCCCTAAACTAAAGCAACGCTGGCCTAGTATCGCAGCGACAACCAGTCGC
AGTGCCGAGTTATGCGCACTGCAACAATCGGTACTCGATGAAGAAATAGCCCTGAAGCTCCCCAATATTCAGCTTAGAAA
GGGCAATGATTGGGTGCTTGATCTCGAAAGGTTAGCGCAGTACTCCAGTGCTTGGCAGTCGTTATTACTGCGCGGACACA
TGGATGCACTAGGTTTTGCCCCCCCATCTAAGGTACAAGGGGAGCAGATCTTAGCCCAGCTTCTTGATGCGAAAGCCGAT
GCTAATGTCGAGATCCGCTTAACTGACGTTATCATTAAGCGTTTTAAGAGCGAAGGCTATTTTTTATTACCGCAAAAAAA
TGCCCAATACGCAGCGGATAAAAAAGGGTTGTCGAATATTCAACTTAACAAAATTGAAGAGCTGATTAATCGAGTCATTG
AGTTACCAACTGGAGATAATTTTTCGACAGAGGCCTGTCAAACTGGGCTTCGTCTTAGGCTGCCATTGTCATCAGAGCAA
GTCTCTATCGAATTTGCCCTGCCTGGTAGTACTCGTTGTCACCCCCATAACCGCAGCAAGGGACGTGAGCTTAAAAAGGT
ATGGCAAGAGTGTGGGCTTGCTCCGTGGAAGCGTGGCACAGTACCGCTGCTTTGTTATGACGGTAAGCTAGTGGCTGCAG
TGGGATACTGGATAGAAAAGTCTTATTTGGCGACCGCTGACGATGTCGGGCTTGTTCTACATATACGATAG

Upstream 100 bases:

>100_bases
TTTACCTTAGGTGATAACTGGCGGGTTAATCCAACCGATGAGTTGATGTTGGCGCTCGAGTCGTTAACGGGGTCTGGCAA
GGTACGGATCGTTTTTTAGC

Downstream 100 bases:

>100_bases
CACTGACGTCCTGCCGCCTTCGCCTCATATAGCGCATTATCAGCACGCTGGTAAAGACTCTCTTCAGTATCATTATGTCG
CCACTGCGCCACACCCATGC

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MNIVTLIQACIQQLPSKVAAAVKVDNSQEQNQAAINKPKPKLVLAYSGGVDSEILAHGLAQFAKVSPHFECLLVHVHHGL
SANADTWAEHCLTQAKHYDLDCTVKRVEVKLAPRLSVEAQARSVRYEALLSELSNNDILMTAHHQDDQLETVLLALKRGL
GPKGLSAMGKVQRFNQQHWIIRPLLDCSKDEIEAYAKRYEITHIQDESNFDDKYDRNFLRLDVIPKLKQRWPSIAATTSR
SAELCALQQSVLDEEIALKLPNIQLRKGNDWVLDLERLAQYSSAWQSLLLRGHMDALGFAPPSKVQGEQILAQLLDAKAD
ANVEIRLTDVIIKRFKSEGYFLLPQKNAQYAADKKGLSNIQLNKIEELINRVIELPTGDNFSTEACQTGLRLRLPLSSEQ
VSIEFALPGSTRCHPHNRSKGRELKKVWQECGLAPWKRGTVPLLCYDGKLVAAVGYWIEKSYLATADDVGLVLHIR

Sequences:

>Translated_476_residues
MNIVTLIQACIQQLPSKVAAAVKVDNSQEQNQAAINKPKPKLVLAYSGGVDSEILAHGLAQFAKVSPHFECLLVHVHHGL
SANADTWAEHCLTQAKHYDLDCTVKRVEVKLAPRLSVEAQARSVRYEALLSELSNNDILMTAHHQDDQLETVLLALKRGL
GPKGLSAMGKVQRFNQQHWIIRPLLDCSKDEIEAYAKRYEITHIQDESNFDDKYDRNFLRLDVIPKLKQRWPSIAATTSR
SAELCALQQSVLDEEIALKLPNIQLRKGNDWVLDLERLAQYSSAWQSLLLRGHMDALGFAPPSKVQGEQILAQLLDAKAD
ANVEIRLTDVIIKRFKSEGYFLLPQKNAQYAADKKGLSNIQLNKIEELINRVIELPTGDNFSTEACQTGLRLRLPLSSEQ
VSIEFALPGSTRCHPHNRSKGRELKKVWQECGLAPWKRGTVPLLCYDGKLVAAVGYWIEKSYLATADDVGLVLHIR
>Mature_476_residues
MNIVTLIQACIQQLPSKVAAAVKVDNSQEQNQAAINKPKPKLVLAYSGGVDSEILAHGLAQFAKVSPHFECLLVHVHHGL
SANADTWAEHCLTQAKHYDLDCTVKRVEVKLAPRLSVEAQARSVRYEALLSELSNNDILMTAHHQDDQLETVLLALKRGL
GPKGLSAMGKVQRFNQQHWIIRPLLDCSKDEIEAYAKRYEITHIQDESNFDDKYDRNFLRLDVIPKLKQRWPSIAATTSR
SAELCALQQSVLDEEIALKLPNIQLRKGNDWVLDLERLAQYSSAWQSLLLRGHMDALGFAPPSKVQGEQILAQLLDAKAD
ANVEIRLTDVIIKRFKSEGYFLLPQKNAQYAADKKGLSNIQLNKIEELINRVIELPTGDNFSTEACQTGLRLRLPLSSEQ
VSIEFALPGSTRCHPHNRSKGRELKKVWQECGLAPWKRGTVPLLCYDGKLVAAVGYWIEKSYLATADDVGLVLHIR

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=429, Percent_Identity=38.2284382284382, Blast_Score=266, Evalue=3e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 53365; Mature: 53365

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIVTLIQACIQQLPSKVAAAVKVDNSQEQNQAAINKPKPKLVLAYSGGVDSEILAHGLA
CCHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHCCCCCCCEEEEECCCCCHHHHHHHHH
QFAKVSPHFECLLVHVHHGLSANADTWAEHCLTQAKHYDLDCTVKRVEVKLAPRLSVEAQ
HHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCHHH
ARSVRYEALLSELSNNDILMTAHHQDDQLETVLLALKRGLGPKGLSAMGKVQRFNQQHWI
HHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEE
IRPLLDCSKDEIEAYAKRYEITHIQDESNFDDKYDRNFLRLDVIPKLKQRWPSIAATTSR
EHHHHCCCHHHHHHHHHHHEEEEECCCCCCCHHHCCCEEEEECCHHHHHHCCCCEECCCC
SAELCALQQSVLDEEIALKLPNIQLRKGNDWVLDLERLAQYSSAWQSLLLRGHMDALGFA
CHHHHHHHHHHCCHHHEEECCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHCCHHHHCCC
PPSKVQGEQILAQLLDAKADANVEIRLTDVIIKRFKSEGYFLLPQKNAQYAADKKGLSNI
CCCCCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCCCC
QLNKIEELINRVIELPTGDNFSTEACQTGLRLRLPLSSEQVSIEFALPGSTRCHPHNRSK
HHHHHHHHHHHHHCCCCCCCCCHHHHHCCCEEEECCCCCEEEEEEECCCCCCCCCCCCCC
GRELKKVWQECGLAPWKRGTVPLLCYDGKLVAAVGYWIEKSYLATADDVGLVLHIR
CHHHHHHHHHCCCCCCCCCCEEEEEECCEEEEHHHHHHHHHHCCCCCCCCEEEEEC
>Mature Secondary Structure
MNIVTLIQACIQQLPSKVAAAVKVDNSQEQNQAAINKPKPKLVLAYSGGVDSEILAHGLA
CCHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHCCCCCCCEEEEECCCCCHHHHHHHHH
QFAKVSPHFECLLVHVHHGLSANADTWAEHCLTQAKHYDLDCTVKRVEVKLAPRLSVEAQ
HHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCHHH
ARSVRYEALLSELSNNDILMTAHHQDDQLETVLLALKRGLGPKGLSAMGKVQRFNQQHWI
HHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEE
IRPLLDCSKDEIEAYAKRYEITHIQDESNFDDKYDRNFLRLDVIPKLKQRWPSIAATTSR
EHHHHCCCHHHHHHHHHHHEEEEECCCCCCCHHHCCCEEEEECCHHHHHHCCCCEECCCC
SAELCALQQSVLDEEIALKLPNIQLRKGNDWVLDLERLAQYSSAWQSLLLRGHMDALGFA
CHHHHHHHHHHCCHHHEEECCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHCCHHHHCCC
PPSKVQGEQILAQLLDAKADANVEIRLTDVIIKRFKSEGYFLLPQKNAQYAADKKGLSNI
CCCCCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCCCC
QLNKIEELINRVIELPTGDNFSTEACQTGLRLRLPLSSEQVSIEFALPGSTRCHPHNRSK
HHHHHHHHHHHHHCCCCCCCCCHHHHHCCCEEEECCCCCEEEEEEECCCCCCCCCCCCCC
GRELKKVWQECGLAPWKRGTVPLLCYDGKLVAAVGYWIEKSYLATADDVGLVLHIR
CHHHHHHHHHCCCCCCCCCCEEEEEECCEEEEHHHHHHHHHHCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA