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The map label for this gene is kefB [H]

Identifier: 167624877

GI number: 167624877

Start: 3603027

End: 3604976

Strand: Reverse

Name: kefB [H]

Synonym: Shal_2963

Alternate gene names: 167624877

Gene position: 3604976-3603027 (Counterclockwise)

Preceding gene: 167624879

Following gene: 167624876

Centisome position: 68.97

GC content: 47.13

Gene sequence:

>1950_bases
ATGGAACATGGCTTTCTCATTCAAATTCTACTGATGCTGGTCATTGCAATTGTTTCAATTGCTATACTCCGACGTGCAGG
CTTGCCAGCGATCTTAGCTTACCTGGTGACCGGAGTGATAAGTGGTCCAAGTGGCTTTCACTGGTTTACCCAGCATCAGA
TGCAGTCAGTTGCCGAACTCGGTGTCGTGTTACTGATGTTTAGTCTAGGACTCGAGTTTTCCTTGCCTAGGCTTTGGGCA
ATGCGTCGCACCGTATTTGGCTTAGGTAGTGCTCAAGTCGTCGTGACCACCGTTTTAAGCATGGGAATTGCGCTGTTATG
TAATCTCAGCGTGACCGAGTCGGTTGTTGTTGGTGCTGCTATCGCACTGTCATCGACTGCCATTGTCCTCAAGTTACTCA
ATGAGCAAGGCTGGTTAAGGCGCCGACATGGTGAGCTTTCTGTCAGTGTGCTGTTATTTCAAGATATTGCCGTTGTTCCC
TTATTAATTTTGCTGCCCATTCTAGCCAGTGATGGCGAGGCGCTATTATGGAACGATGTATTTTTTGCCTTGCTAAAGGG
CGTATTGGCGTTTATTGCATTGATGGCTTTTGGTAAGTGGGCTTTGCCTAAGCTGTTTGACGAGGTGGCTCGCTCACGTT
CAAATGAGCTATTTGTCCTTTCCACTCTAGTGGTTGCTCTGGTAACTGGCGCCATTACTCAGTGGCTCGGTTTATCGATG
GCATTAGGTGCCTTTATGGCGGGTATGCTGCTTGGTGAAAGTCAGTACCGCCGCCAGCTTGAGGCTGACATTCGTCCCTT
TCGGGATCTATTGATGGGGCTGTTTTTTATTTCCATCGGTATGTTGTTGGATTTCCAGTTGGTCGCCCAGTACTGGTGGC
AAATCATCTTTATCCTTATCGCAGTGATCTTAGGTAAAGCACTGGTGGTGTTCTCTCTGTTGAAGGCTGCTAAAGAGTCT
TTTCGTTCCTCGGTAGCCACAGCATTGAGTCTAGCGCAGGTGGGAGAGTTCAGTTTTGTGGTCTTGGCTTTGGCTGTTAA
CTATCAATTGCTCGACAACAAAACCAGTACCACATTGGTTATGGTTGCCGTGCTATCTATGGCATTAGCCCCCTGGTTGG
TAAGGCACAGTGTCGATATAGCCAGAATGCTGCAAGGCTTGAAACCTAAGGCTGATAGCAGTGAGTCGATTAATGTCCAA
GTCGATGCTAAAGGCGATCTGGTACTCATCTTAGGCTACGGTCGAGTGGGGCAAACCATTGCAAGGTTCATGAAAACTGA
AGCTATTCCATATTTAGTTCTGGATCTCGACCCTAAAAGAGTCTCTGAGGCGAGAACCGCTGGAGAGCCTGTTTATTTTG
GTGATGTGTGTAAGAGAAGTATTCTAAAACAAGCAAAGGTGCGTGAAGCAAAGTTGATCGTGATTACTTTTAGCAGTGAG
CGAATACTAGAAGAGGTTCTACCCTTATGCCGTCAACTGGCTCCAGAGGCTAAAATCTTGGTTCGTACACGAGATGACTC
TGGTATGGAAGAGTTAGAAGAAGCTGGGGCAAGTCAGGTTATTCCCGAAACACTCGAGGGTAGCTTAATGTTAGTGTCTC
AAGTTTTGTACCAATGTGGAGTGCCGCTGGCACGGATCCTTAAACGGCTTGAATCTGAGCGGCGAAACCATTATCAATAC
TTACATGGCTTTTTTTCCGGTGCTGAAACCGACTTTACCTTGGAGCAGCTACACGCAGTCGCTCTACCAAAAGGTGCCAA
TGCTGTAGGGCTGACCTTGTCACAGATCCCATGGGAAAAATTAAGAGTCGAGCTTAGAGCGGTAAGGCGTGCAGGTGCAG
AAGTTGAGGATCCTGAACTCGATTGGCAGATAAAGTCTGGCGATATTCTTATCTTGCTTGGTAAGCCTAGACGCATGGAA
AAAGCCGAGATCTTTTTATTGCAAGGCTAA

Upstream 100 bases:

>100_bases
GTCTTTATTTGTCATTCAGTTACTCTCTAGTTAAGTGAATGGAAAATATTAGTGTCGGCATTTTGACGAATGTCTTTGGG
TTAAGGAGTACACCGCACAG

Downstream 100 bases:

>100_bases
GCCTCTCTGTAACCCTCATTCGGTAAAGTTTCGCTATAGTTAATGGTTGTAAAACAGGTTCAGCCAAACTCTTGATGCTG
AATATTCAAGGAGTGCAACC

Product: potassium efflux system protein

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 649; Mature: 649

Protein sequence:

>649_residues
MEHGFLIQILLMLVIAIVSIAILRRAGLPAILAYLVTGVISGPSGFHWFTQHQMQSVAELGVVLLMFSLGLEFSLPRLWA
MRRTVFGLGSAQVVVTTVLSMGIALLCNLSVTESVVVGAAIALSSTAIVLKLLNEQGWLRRRHGELSVSVLLFQDIAVVP
LLILLPILASDGEALLWNDVFFALLKGVLAFIALMAFGKWALPKLFDEVARSRSNELFVLSTLVVALVTGAITQWLGLSM
ALGAFMAGMLLGESQYRRQLEADIRPFRDLLMGLFFISIGMLLDFQLVAQYWWQIIFILIAVILGKALVVFSLLKAAKES
FRSSVATALSLAQVGEFSFVVLALAVNYQLLDNKTSTTLVMVAVLSMALAPWLVRHSVDIARMLQGLKPKADSSESINVQ
VDAKGDLVLILGYGRVGQTIARFMKTEAIPYLVLDLDPKRVSEARTAGEPVYFGDVCKRSILKQAKVREAKLIVITFSSE
RILEEVLPLCRQLAPEAKILVRTRDDSGMEELEEAGASQVIPETLEGSLMLVSQVLYQCGVPLARILKRLESERRNHYQY
LHGFFSGAETDFTLEQLHAVALPKGANAVGLTLSQIPWEKLRVELRAVRRAGAEVEDPELDWQIKSGDILILLGKPRRME
KAEIFLLQG

Sequences:

>Translated_649_residues
MEHGFLIQILLMLVIAIVSIAILRRAGLPAILAYLVTGVISGPSGFHWFTQHQMQSVAELGVVLLMFSLGLEFSLPRLWA
MRRTVFGLGSAQVVVTTVLSMGIALLCNLSVTESVVVGAAIALSSTAIVLKLLNEQGWLRRRHGELSVSVLLFQDIAVVP
LLILLPILASDGEALLWNDVFFALLKGVLAFIALMAFGKWALPKLFDEVARSRSNELFVLSTLVVALVTGAITQWLGLSM
ALGAFMAGMLLGESQYRRQLEADIRPFRDLLMGLFFISIGMLLDFQLVAQYWWQIIFILIAVILGKALVVFSLLKAAKES
FRSSVATALSLAQVGEFSFVVLALAVNYQLLDNKTSTTLVMVAVLSMALAPWLVRHSVDIARMLQGLKPKADSSESINVQ
VDAKGDLVLILGYGRVGQTIARFMKTEAIPYLVLDLDPKRVSEARTAGEPVYFGDVCKRSILKQAKVREAKLIVITFSSE
RILEEVLPLCRQLAPEAKILVRTRDDSGMEELEEAGASQVIPETLEGSLMLVSQVLYQCGVPLARILKRLESERRNHYQY
LHGFFSGAETDFTLEQLHAVALPKGANAVGLTLSQIPWEKLRVELRAVRRAGAEVEDPELDWQIKSGDILILLGKPRRME
KAEIFLLQG
>Mature_649_residues
MEHGFLIQILLMLVIAIVSIAILRRAGLPAILAYLVTGVISGPSGFHWFTQHQMQSVAELGVVLLMFSLGLEFSLPRLWA
MRRTVFGLGSAQVVVTTVLSMGIALLCNLSVTESVVVGAAIALSSTAIVLKLLNEQGWLRRRHGELSVSVLLFQDIAVVP
LLILLPILASDGEALLWNDVFFALLKGVLAFIALMAFGKWALPKLFDEVARSRSNELFVLSTLVVALVTGAITQWLGLSM
ALGAFMAGMLLGESQYRRQLEADIRPFRDLLMGLFFISIGMLLDFQLVAQYWWQIIFILIAVILGKALVVFSLLKAAKES
FRSSVATALSLAQVGEFSFVVLALAVNYQLLDNKTSTTLVMVAVLSMALAPWLVRHSVDIARMLQGLKPKADSSESINVQ
VDAKGDLVLILGYGRVGQTIARFMKTEAIPYLVLDLDPKRVSEARTAGEPVYFGDVCKRSILKQAKVREAKLIVITFSSE
RILEEVLPLCRQLAPEAKILVRTRDDSGMEELEEAGASQVIPETLEGSLMLVSQVLYQCGVPLARILKRLESERRNHYQY
LHGFFSGAETDFTLEQLHAVALPKGANAVGLTLSQIPWEKLRVELRAVRRAGAEVEDPELDWQIKSGDILILLGKPRRME
KAEIFLLQG

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=371, Percent_Identity=26.1455525606469, Blast_Score=95, Evalue=2e-19,
Organism=Escherichia coli, GI1789749, Length=543, Percent_Identity=35.9116022099448, Blast_Score=273, Evalue=3e-74,
Organism=Escherichia coli, GI1786232, Length=560, Percent_Identity=31.4285714285714, Blast_Score=261, Evalue=8e-71,
Organism=Escherichia coli, GI1786685, Length=507, Percent_Identity=31.5581854043393, Blast_Score=203, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6322367, Length=532, Percent_Identity=21.2406015037594, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 71504; Mature: 71504

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHGFLIQILLMLVIAIVSIAILRRAGLPAILAYLVTGVISGPSGFHWFTQHQMQSVAEL
CCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
GVVLLMFSLGLEFSLPRLWAMRRTVFGLGSAQVVVTTVLSMGIALLCNLSVTESVVVGAA
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
IALSSTAIVLKLLNEQGWLRRRHGELSVSVLLFQDIAVVPLLILLPILASDGEALLWNDV
HHHHHHHHHHHHHHCCCHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHH
FFALLKGVLAFIALMAFGKWALPKLFDEVARSRSNELFVLSTLVVALVTGAITQWLGLSM
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
ALGAFMAGMLLGESQYRRQLEADIRPFRDLLMGLFFISIGMLLDFQLVAQYWWQIIFILI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVILGKALVVFSLLKAAKESFRSSVATALSLAQVGEFSFVVLALAVNYQLLDNKTSTTLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHH
MVAVLSMALAPWLVRHSVDIARMLQGLKPKADSSESINVQVDAKGDLVLILGYGRVGQTI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEEEECCHHHHHH
ARFMKTEAIPYLVLDLDPKRVSEARTAGEPVYFGDVCKRSILKQAKVREAKLIVITFSSE
HHHHHHCCCCEEEEECCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCEEEEEEECHH
RILEEVLPLCRQLAPEAKILVRTRDDSGMEELEEAGASQVIPETLEGSLMLVSQVLYQCG
HHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHCC
VPLARILKRLESERRNHYQYLHGFFSGAETDFTLEQLHAVALPKGANAVGLTLSQIPWEK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHCCHHH
LRVELRAVRRAGAEVEDPELDWQIKSGDILILLGKPRRMEKAEIFLLQG
HHHHHHHHHHCCCCCCCCCCCEEECCCCEEEEECCCCCCCCCEEEEECC
>Mature Secondary Structure
MEHGFLIQILLMLVIAIVSIAILRRAGLPAILAYLVTGVISGPSGFHWFTQHQMQSVAEL
CCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
GVVLLMFSLGLEFSLPRLWAMRRTVFGLGSAQVVVTTVLSMGIALLCNLSVTESVVVGAA
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
IALSSTAIVLKLLNEQGWLRRRHGELSVSVLLFQDIAVVPLLILLPILASDGEALLWNDV
HHHHHHHHHHHHHHCCCHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHH
FFALLKGVLAFIALMAFGKWALPKLFDEVARSRSNELFVLSTLVVALVTGAITQWLGLSM
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
ALGAFMAGMLLGESQYRRQLEADIRPFRDLLMGLFFISIGMLLDFQLVAQYWWQIIFILI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVILGKALVVFSLLKAAKESFRSSVATALSLAQVGEFSFVVLALAVNYQLLDNKTSTTLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHH
MVAVLSMALAPWLVRHSVDIARMLQGLKPKADSSESINVQVDAKGDLVLILGYGRVGQTI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEEEECCHHHHHH
ARFMKTEAIPYLVLDLDPKRVSEARTAGEPVYFGDVCKRSILKQAKVREAKLIVITFSSE
HHHHHHCCCCEEEEECCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCEEEEEEECHH
RILEEVLPLCRQLAPEAKILVRTRDDSGMEELEEAGASQVIPETLEGSLMLVSQVLYQCG
HHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHCC
VPLARILKRLESERRNHYQYLHGFFSGAETDFTLEQLHAVALPKGANAVGLTLSQIPWEK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHCCHHH
LRVELRAVRRAGAEVEDPELDWQIKSGDILILLGKPRRMEKAEIFLLQG
HHHHHHHHHHCCCCCCCCCCCEEECCCCEEEEECCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA