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The map label for this gene is 167624873

Identifier: 167624873

GI number: 167624873

Start: 3597523

End: 3598467

Strand: Reverse

Name: 167624873

Synonym: Shal_2959

Alternate gene names: NA

Gene position: 3598467-3597523 (Counterclockwise)

Preceding gene: 167624876

Following gene: 167624867

Centisome position: 68.84

GC content: 46.67

Gene sequence:

>945_bases
ATGAAAAAAGTCGCCATCATTCAAGAAGCCCCTTGTGTGCTAGATAAGAAAGCAACGATTCAAAAAGCCGTAGAAATTGT
TCATTCGGTATCAGAAAAAGGTGCAGAACTTATTGTTTTTCCTGAGGCATTCATTCCAGGATACCCAGCATGGATATGGC
GCTTAAGACCGGGGGCGGACTGGGGCGTATGTGAAGCGTTACATAGGCGTTTGCTACAAAACTCGGTGGATCTGTCATCC
GATGATCTTAAGCCGCTACAAGAGGCTGCTAAACAGAGAGCTGTGACCCTAGTTTGCGGATTAAACGAGCGAGATTATGC
CAATAGCCAGTCGACCATCTATAACACCGCCATTACTATTGATCACCAAGGGCAAATCGTTAACCATCACCGTAAACTGA
TGCCGACCAATCCAGAGCGTATGGTTTGGGGCTTTGGCGATGGCCATGGGTTAAACGTGATTACTACGCCAGTTGGAAAA
ATTGCTAGCCTCATATGCTGGGAAAATTATATGCCTCTGGCGAGGTACGCCCTGTATTCACAGGGGGTTGAAATATATAT
CGCACCGACCTATGACAGCGGTGATGCTTGGATTAGCACTATGCAGCATATCGCTAGAGAGGGAAAATGTTGGGTACTGT
GCTGTGGCGTAGCGCTGCAAAGAAAAGATCTGCCAGAAGATTTTCCTGATCTCGATAGACTCTACCCGGCAGATGAGGAT
TGGATCAATCCCGGAGATTCGCTCATCGTATCCCCAAGCGGCGAAATCATAGCTGGCCCCATGTCTAAAGAGAAGGGCTA
TATACTGCTAGATATTGATGTTGAAAAGGCGGCTAGCTCAAAGCGTGCGCTGGATGTTGCAGGGCATTATTCGAGGCCAG
ATGTTTTTATACTTGAGGTCGATAAGTCTCGCCAGTCACCAGTAAGATTTAAGAATAACAGTTAA

Upstream 100 bases:

>100_bases
CATGACATCTGAAACTCGAGTTTAACTTTTCTGGGGCTCATCCTTCTATGGGTCGATGAAAACGCCATTAACTCAACTGT
TCATTGGAAGGGATCAAATC

Downstream 100 bases:

>100_bases
GAATGACAGTTAAGTACTCGAGTTAATAAGCGAAACCTTATTCCGTTGTGTTAATTTTGAGTAAGGACGTCTTTATATAG
CAACATATAACGGCTATTCC

Product: Nitrilase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKKVAIIQEAPCVLDKKATIQKAVEIVHSVSEKGAELIVFPEAFIPGYPAWIWRLRPGADWGVCEALHRRLLQNSVDLSS
DDLKPLQEAAKQRAVTLVCGLNERDYANSQSTIYNTAITIDHQGQIVNHHRKLMPTNPERMVWGFGDGHGLNVITTPVGK
IASLICWENYMPLARYALYSQGVEIYIAPTYDSGDAWISTMQHIAREGKCWVLCCGVALQRKDLPEDFPDLDRLYPADED
WINPGDSLIVSPSGEIIAGPMSKEKGYILLDIDVEKAASSKRALDVAGHYSRPDVFILEVDKSRQSPVRFKNNS

Sequences:

>Translated_314_residues
MKKVAIIQEAPCVLDKKATIQKAVEIVHSVSEKGAELIVFPEAFIPGYPAWIWRLRPGADWGVCEALHRRLLQNSVDLSS
DDLKPLQEAAKQRAVTLVCGLNERDYANSQSTIYNTAITIDHQGQIVNHHRKLMPTNPERMVWGFGDGHGLNVITTPVGK
IASLICWENYMPLARYALYSQGVEIYIAPTYDSGDAWISTMQHIAREGKCWVLCCGVALQRKDLPEDFPDLDRLYPADED
WINPGDSLIVSPSGEIIAGPMSKEKGYILLDIDVEKAASSKRALDVAGHYSRPDVFILEVDKSRQSPVRFKNNS
>Mature_314_residues
MKKVAIIQEAPCVLDKKATIQKAVEIVHSVSEKGAELIVFPEAFIPGYPAWIWRLRPGADWGVCEALHRRLLQNSVDLSS
DDLKPLQEAAKQRAVTLVCGLNERDYANSQSTIYNTAITIDHQGQIVNHHRKLMPTNPERMVWGFGDGHGLNVITTPVGK
IASLICWENYMPLARYALYSQGVEIYIAPTYDSGDAWISTMQHIAREGKCWVLCCGVALQRKDLPEDFPDLDRLYPADED
WINPGDSLIVSPSGEIIAGPMSKEKGYILLDIDVEKAASSKRALDVAGHYSRPDVFILEVDKSRQSPVRFKNNS

Specific function: Acts on aliphatic nitriles such as acrylonitrile, crotononitrile and glutaronitrile [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17557111, Length=309, Percent_Identity=40.1294498381877, Blast_Score=218, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6322027, Length=201, Percent_Identity=37.8109452736318, Blast_Score=129, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000132
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.5.7 [H]

Molecular weight: Translated: 35043; Mature: 35043

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00920 NITRIL_CHT_1 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVAIIQEAPCVLDKKATIQKAVEIVHSVSEKGAELIVFPEAFIPGYPAWIWRLRPGAD
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECHHHCCCCCEEEEEECCCCC
WGVCEALHRRLLQNSVDLSSDDLKPLQEAAKQRAVTLVCGLNERDYANSQSTIYNTAITI
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCEEEEEECCCCCCCCCCCCEEEEEEEEE
DHQGQIVNHHRKLMPTNPERMVWGFGDGHGLNVITTPVGKIASLICWENYMPLARYALYS
ECCCCCHHCCHHCCCCCCCEEEEECCCCCCCEEEECCHHHHHHHHHHHCCCHHHHHHHHH
QGVEIYIAPTYDSGDAWISTMQHIAREGKCWVLCCGVALQRKDLPEDFPDLDRLYPADED
CCEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHCCCCCCCCCHHHCCCCCCC
WINPGDSLIVSPSGEIIAGPMSKEKGYILLDIDVEKAASSKRALDVAGHYSRPDVFILEV
CCCCCCCEEECCCCCEEECCCCCCCCEEEEEEEHHHHCCCCCEEEECCCCCCCCEEEEEE
DKSRQSPVRFKNNS
CCCCCCCCCCCCCC
>Mature Secondary Structure
MKKVAIIQEAPCVLDKKATIQKAVEIVHSVSEKGAELIVFPEAFIPGYPAWIWRLRPGAD
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECHHHCCCCCEEEEEECCCCC
WGVCEALHRRLLQNSVDLSSDDLKPLQEAAKQRAVTLVCGLNERDYANSQSTIYNTAITI
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCEEEEEECCCCCCCCCCCCEEEEEEEEE
DHQGQIVNHHRKLMPTNPERMVWGFGDGHGLNVITTPVGKIASLICWENYMPLARYALYS
ECCCCCHHCCHHCCCCCCCEEEEECCCCCCCEEEECCHHHHHHHHHHHCCCHHHHHHHHH
QGVEIYIAPTYDSGDAWISTMQHIAREGKCWVLCCGVALQRKDLPEDFPDLDRLYPADED
CCEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHCCCCCCCCCHHHCCCCCCC
WINPGDSLIVSPSGEIIAGPMSKEKGYILLDIDVEKAASSKRALDVAGHYSRPDVFILEV
CCCCCCCEEECCCCCEEECCCCCCCCEEEEEEEHHHHCCCCCEEEECCCCCCCCEEEEEE
DKSRQSPVRFKNNS
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1390687 [H]