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The map label for this gene is nrfA [H]

Identifier: 167624832

GI number: 167624832

Start: 3548075

End: 3549460

Strand: Reverse

Name: nrfA [H]

Synonym: Shal_2918

Alternate gene names: 167624832

Gene position: 3549460-3548075 (Counterclockwise)

Preceding gene: 167624834

Following gene: 167624830

Centisome position: 67.91

GC content: 46.32

Gene sequence:

>1386_bases
ATGAAATCATACAAAGTTTCCTTGATAGCAGGAGTCTGCATCGGCATTTTAAGTAGTGCTGCGGTGGCAAAAACGAATCC
AGCAGATAATGCCCAGTACGAGAAGCGCTTTCCTAAGCAATATCAAAGCTGGGAAAAAACAGCTGAGAGTAAAGAGGTTA
CCAATGCATTAGCCGCCGATCCTAACTTGGTTATTCTGTGGGCGGGTTACGGGTTTTCCAAAGATTACAACGCCCCTAGA
GGGCATCAGTATGCTATTACCGATGTGAGAACCACGCTACGAACTGGTGCGCCGATGAAAGCGGATGATGGTCCAATGCC
AATGGCTTGTTGGAGTTGTAAAAGCCCAGATGTACCGCGAGTGATTGAGGAACAGGGGGAAGCTGAGTACTTCAAAGGAA
AGTGGGCTAAAGGAGGCCCTGAAATTGTCAATGTGATTGGTTGTGCTGATTGTCATAAGCCAGGAACATCGAAGTTAAGA
GTTGCACGTCCATTTGCCGAGCGCGCCTTTGCGGCAATAGGTACACCGTTCGATAAGGCAGAAAAGAGCGAAAAACAAAA
TATGGTGTGCGGACAGTGTCACGTGGAGTATTACTTTGAAAAAACAGCTGAGCATCCAAATTGGGTTAAATTCCCTTGGG
ATGATGGCACCTCAGTCGAAGAGATGGAAGCTTACTATGACAAGCTGAAGTTCAAAGACTGGACTCACCAACTATCAAAA
GCGCCGATGTTAAAAGCTCAGCATCCAGGTTATGAAACTACAGCATTGGGGATGCATGGTGAGATGGGAATATCATGCAC
CGATTGCCATATGCCACGGGTAACCAATGCTGAAGGAAAAGAATATACCGATCATAATGTTGGTAATCCATTTGATAAAT
TTGAGTTTACTTGTGCCACCTGTCACGAACAAACCAAAGAGCAGTTGCAAGGTCTAGTGCAGGCTAATAAAGATAAGGTT
AATACCCTTAAGCTTAAGGCGGAGGCTGAGTTAGTGAAGGCTCACTTTGAAGCTGATGCAGCCTGGAAAGCGGGAGCGAC
AGAGCAGCAAATGGCTGCGGCGTTAACGGACATTCGTCATGCTCAATGGCGCTGGGATTTTGCCATTGCTTCTCATGGTA
TTGCAGCTCATGCGCCAGAAGAGGGACTGCGTATTTTAGAAACATCATTAGCTAAAACCGCAGAGGCCCGCGCTAAGCTC
GCCAAAGTATTGGCAGCTCAAGGTGTTAAAACGGCTGTAGTCATTCCAGATATCTCTACTGAAGCGAAAGCACAGGCTCT
TTTAGGCATGGATATGGAAAAAATGCGTGCAGATAAAGCCATCTTTATCGAGAAAGTGGTACCTAAGTGGGAGGCTGAAT
ATAAGGCGACTAATGGCGGTGAGTAG

Upstream 100 bases:

>100_bases
AAAATAAAAAGAAAATTTAATTGCGGTAGCTTTTGACAACGTGGTTATAGCGACTACATTTTATATATCTAAATACCCTC
ACATAATGGATGGATAACCT

Downstream 100 bases:

>100_bases
ACTTTAAGGATTGTTACTTAAATAAAAATCCGAGAAGTTGGCTTCTCGGATTTTTTATCTCATGATGATACTGAATTTAT
ATATCGTTATCTGTGCGAAT

Product: nitrite reductase

Products: NA

Alternate protein names: Ammonia-forming cytochrome c nitrite reductase; Cytochrome c nitrite reductase [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MKSYKVSLIAGVCIGILSSAAVAKTNPADNAQYEKRFPKQYQSWEKTAESKEVTNALAADPNLVILWAGYGFSKDYNAPR
GHQYAITDVRTTLRTGAPMKADDGPMPMACWSCKSPDVPRVIEEQGEAEYFKGKWAKGGPEIVNVIGCADCHKPGTSKLR
VARPFAERAFAAIGTPFDKAEKSEKQNMVCGQCHVEYYFEKTAEHPNWVKFPWDDGTSVEEMEAYYDKLKFKDWTHQLSK
APMLKAQHPGYETTALGMHGEMGISCTDCHMPRVTNAEGKEYTDHNVGNPFDKFEFTCATCHEQTKEQLQGLVQANKDKV
NTLKLKAEAELVKAHFEADAAWKAGATEQQMAAALTDIRHAQWRWDFAIASHGIAAHAPEEGLRILETSLAKTAEARAKL
AKVLAAQGVKTAVVIPDISTEAKAQALLGMDMEKMRADKAIFIEKVVPKWEAEYKATNGGE

Sequences:

>Translated_461_residues
MKSYKVSLIAGVCIGILSSAAVAKTNPADNAQYEKRFPKQYQSWEKTAESKEVTNALAADPNLVILWAGYGFSKDYNAPR
GHQYAITDVRTTLRTGAPMKADDGPMPMACWSCKSPDVPRVIEEQGEAEYFKGKWAKGGPEIVNVIGCADCHKPGTSKLR
VARPFAERAFAAIGTPFDKAEKSEKQNMVCGQCHVEYYFEKTAEHPNWVKFPWDDGTSVEEMEAYYDKLKFKDWTHQLSK
APMLKAQHPGYETTALGMHGEMGISCTDCHMPRVTNAEGKEYTDHNVGNPFDKFEFTCATCHEQTKEQLQGLVQANKDKV
NTLKLKAEAELVKAHFEADAAWKAGATEQQMAAALTDIRHAQWRWDFAIASHGIAAHAPEEGLRILETSLAKTAEARAKL
AKVLAAQGVKTAVVIPDISTEAKAQALLGMDMEKMRADKAIFIEKVVPKWEAEYKATNGGE
>Mature_461_residues
MKSYKVSLIAGVCIGILSSAAVAKTNPADNAQYEKRFPKQYQSWEKTAESKEVTNALAADPNLVILWAGYGFSKDYNAPR
GHQYAITDVRTTLRTGAPMKADDGPMPMACWSCKSPDVPRVIEEQGEAEYFKGKWAKGGPEIVNVIGCADCHKPGTSKLR
VARPFAERAFAAIGTPFDKAEKSEKQNMVCGQCHVEYYFEKTAEHPNWVKFPWDDGTSVEEMEAYYDKLKFKDWTHQLSK
APMLKAQHPGYETTALGMHGEMGISCTDCHMPRVTNAEGKEYTDHNVGNPFDKFEFTCATCHEQTKEQLQGLVQANKDKV
NTLKLKAEAELVKAHFEADAAWKAGATEQQMAAALTDIRHAQWRWDFAIASHGIAAHAPEEGLRILETSLAKTAEARAKL
AKVLAAQGVKTAVVIPDISTEAKAQALLGMDMEKMRADKAIFIEKVVPKWEAEYKATNGGE

Specific function: Plays a role in nitrite reduction [H]

COG id: COG3303

COG function: function code P; Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome c-552 family [H]

Homologues:

Organism=Escherichia coli, GI1790506, Length=446, Percent_Identity=58.7443946188341, Blast_Score=551, Evalue=1e-158,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003321
- InterPro:   IPR017570
- InterPro:   IPR011031 [H]

Pfam domain/function: PF02335 Cytochrom_C552 [H]

EC number: =1.7.2.2 [H]

Molecular weight: Translated: 50972; Mature: 50972

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSYKVSLIAGVCIGILSSAAVAKTNPADNAQYEKRFPKQYQSWEKTAESKEVTNALAAD
CCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHCC
PNLVILWAGYGFSKDYNAPRGHQYAITDVRTTLRTGAPMKADDGPMPMACWSCKSPDVPR
CCEEEEEECCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCH
VIEEQGEAEYFKGKWAKGGPEIVNVIGCADCHKPGTSKLRVARPFAERAFAAIGTPFDKA
HHHHCCCCHHHCCCCCCCCHHHEEEEECCCCCCCCCCCEEEECHHHHHHHHHHCCCHHHH
EKSEKQNMVCGQCHVEYYFEKTAEHPNWVKFPWDDGTSVEEMEAYYDKLKFKDWTHQLSK
HHHHHHCCEEHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHC
APMLKAQHPGYETTALGMHGEMGISCTDCHMPRVTNAEGKEYTDHNVGNPFDKFEFTCAT
CCCCCCCCCCCCEEEECCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHEEHHHH
CHEQTKEQLQGLVQANKDKVNTLKLKAEAELVKAHFEADAAWKAGATEQQMAAALTDIRH
HHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
AQWRWDFAIASHGIAAHAPEEGLRILETSLAKTAEARAKLAKVLAAQGVKTAVVIPDIST
HHEEEEHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
EAKAQALLGMDMEKMRADKAIFIEKVVPKWEAEYKATNGGE
HHHHHHHHCCCHHHHHCCHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKSYKVSLIAGVCIGILSSAAVAKTNPADNAQYEKRFPKQYQSWEKTAESKEVTNALAAD
CCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHCC
PNLVILWAGYGFSKDYNAPRGHQYAITDVRTTLRTGAPMKADDGPMPMACWSCKSPDVPR
CCEEEEEECCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCH
VIEEQGEAEYFKGKWAKGGPEIVNVIGCADCHKPGTSKLRVARPFAERAFAAIGTPFDKA
HHHHCCCCHHHCCCCCCCCHHHEEEEECCCCCCCCCCCEEEECHHHHHHHHHHCCCHHHH
EKSEKQNMVCGQCHVEYYFEKTAEHPNWVKFPWDDGTSVEEMEAYYDKLKFKDWTHQLSK
HHHHHHCCEEHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHC
APMLKAQHPGYETTALGMHGEMGISCTDCHMPRVTNAEGKEYTDHNVGNPFDKFEFTCAT
CCCCCCCCCCCCEEEECCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHEEHHHH
CHEQTKEQLQGLVQANKDKVNTLKLKAEAELVKAHFEADAAWKAGATEQQMAAALTDIRH
HHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
AQWRWDFAIASHGIAAHAPEEGLRILETSLAKTAEARAKLAKVLAAQGVKTAVVIPDIST
HHEEEEHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
EAKAQALLGMDMEKMRADKAIFIEKVVPKWEAEYKATNGGE
HHHHHHHHCCCHHHHHCCHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA