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The map label for this gene is dmlR [H]

Identifier: 167624804

GI number: 167624804

Start: 3514649

End: 3515593

Strand: Reverse

Name: dmlR [H]

Synonym: Shal_2890

Alternate gene names: 167624804

Gene position: 3515593-3514649 (Counterclockwise)

Preceding gene: 167624807

Following gene: 167624801

Centisome position: 67.26

GC content: 42.22

Gene sequence:

>945_bases
ATGGATTTTCCAAGTCGATTATTACTGTTGCTTGAAGTCGCTGAACTGGGCTCATTTAGTAAGGTGGCCGAACAAAGAAA
TGTTGATAGATCTGTTATCTCTAAACAGATCAACCGACTAGAGCATGAACTTGGGGTGCATTTGCTAAATCGCACCACAC
GCTCACTTTCTTTAACCGCAGTGGGTCATGAAATGGTGAGCCAAGCAAAATCATTGCGTACTCTGTTAAATGACTCCCAT
CGATTGGCACAGTATTATCATTCGGAACCCAAAGGCGTACTGCGGATCACCAGCTCGACCTTGTTTGGTCGTCAACATGT
ACAGCAAGCAGTGTTGGTTTTTCAGCAACAATATCCCGATGTTGATATTGAACTGCGTTTAGAAGATAGGCTCATCGATA
TGGTTGGTGAGGGGTTTGATATTGGCTTTCGTATTGGCAAACCAAAAGACTCTAACCTGATTACTAGAAAGATTGCGCGA
AACCGGCTGTTGATTGTGGCCGCTCCAGAGTTCATCAAAAAACATGGTGAAATAAAGACGCTTGAACAACTAGAGGCTCT
ACCCGCTGCGGTTTACGCCGCCCCAGGGCTACTGATCGATAAGTTCTCTTATCTTGATGCCAACAACGAAACTAAGCAGT
TGCAATTGAATGTGGTTTATAAAGTTAATGATGTTGAAATGATCACCCAAACGGCTGTAGCGGGCAACATGCTGGCTGTG
GTTGCCGCGCAGATGATTGATAACGAAATTCTAGAGCAGAAGCTTATTCCAGTAATGACTCAGCTTCATTTAAATGATCT
AGGTACATTTTATGCGGTGTATCCGCATCGAGATGCTCCTATCAAAACTAAATTATTTATTAACACGCTTAAAGCTATCA
TTGGAGAAGAAGTACCAATTTGGGAAAAGAAAATTCCCAACTTTGATAAAATGTATGGGCATTAA

Upstream 100 bases:

>100_bases
TTTAAGCCATTAAAGTGGAGCTACTATAAAGTTGTTATAAGCTAAGAAAAATGCTTAAACTGGCACTTGATTGGTGCTAA
AAATGCAACAAAGAGGGTGT

Downstream 100 bases:

>100_bases
TAGTCATCACATATTTAGCTAAGTCTTAGCTTTGATATGCTTTTAGGTTCAACGATTGTGAGTATACCGCTGAATCCGTT
CACCATTGCTATTGCCAATA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MDFPSRLLLLLEVAELGSFSKVAEQRNVDRSVISKQINRLEHELGVHLLNRTTRSLSLTAVGHEMVSQAKSLRTLLNDSH
RLAQYYHSEPKGVLRITSSTLFGRQHVQQAVLVFQQQYPDVDIELRLEDRLIDMVGEGFDIGFRIGKPKDSNLITRKIAR
NRLLIVAAPEFIKKHGEIKTLEQLEALPAAVYAAPGLLIDKFSYLDANNETKQLQLNVVYKVNDVEMITQTAVAGNMLAV
VAAQMIDNEILEQKLIPVMTQLHLNDLGTFYAVYPHRDAPIKTKLFINTLKAIIGEEVPIWEKKIPNFDKMYGH

Sequences:

>Translated_314_residues
MDFPSRLLLLLEVAELGSFSKVAEQRNVDRSVISKQINRLEHELGVHLLNRTTRSLSLTAVGHEMVSQAKSLRTLLNDSH
RLAQYYHSEPKGVLRITSSTLFGRQHVQQAVLVFQQQYPDVDIELRLEDRLIDMVGEGFDIGFRIGKPKDSNLITRKIAR
NRLLIVAAPEFIKKHGEIKTLEQLEALPAAVYAAPGLLIDKFSYLDANNETKQLQLNVVYKVNDVEMITQTAVAGNMLAV
VAAQMIDNEILEQKLIPVMTQLHLNDLGTFYAVYPHRDAPIKTKLFINTLKAIIGEEVPIWEKKIPNFDKMYGH
>Mature_314_residues
MDFPSRLLLLLEVAELGSFSKVAEQRNVDRSVISKQINRLEHELGVHLLNRTTRSLSLTAVGHEMVSQAKSLRTLLNDSH
RLAQYYHSEPKGVLRITSSTLFGRQHVQQAVLVFQQQYPDVDIELRLEDRLIDMVGEGFDIGFRIGKPKDSNLITRKIAR
NRLLIVAAPEFIKKHGEIKTLEQLEALPAAVYAAPGLLIDKFSYLDANNETKQLQLNVVYKVNDVEMITQTAVAGNMLAV
VAAQMIDNEILEQKLIPVMTQLHLNDLGTFYAVYPHRDAPIKTKLFINTLKAIIGEEVPIWEKKIPNFDKMYGH

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=296, Percent_Identity=31.4189189189189, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1786401, Length=286, Percent_Identity=28.6713286713287, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1787589, Length=288, Percent_Identity=26.7361111111111, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789639, Length=287, Percent_Identity=25.7839721254355, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1789440, Length=233, Percent_Identity=27.4678111587983, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI145693193, Length=298, Percent_Identity=26.1744966442953, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1787128, Length=178, Percent_Identity=28.6516853932584, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1789173, Length=244, Percent_Identity=26.6393442622951, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1787879, Length=119, Percent_Identity=34.453781512605, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786713, Length=117, Percent_Identity=35.8974358974359, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35574; Mature: 35574

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFPSRLLLLLEVAELGSFSKVAEQRNVDRSVISKQINRLEHELGVHLLNRTTRSLSLTA
CCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHEEEHH
VGHEMVSQAKSLRTLLNDSHRLAQYYHSEPKGVLRITSSTLFGRQHVQQAVLVFQQQYPD
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCC
VDIELRLEDRLIDMVGEGFDIGFRIGKPKDSNLITRKIARNRLLIVAAPEFIKKHGEIKT
CCEEEEHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCEEEEECHHHHHHCCCHHH
LEQLEALPAAVYAAPGLLIDKFSYLDANNETKQLQLNVVYKVNDVEMITQTAVAGNMLAV
HHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHH
VAAQMIDNEILEQKLIPVMTQLHLNDLGTFYAVYPHRDAPIKTKLFINTLKAIIGEEVPI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCC
WEKKIPNFDKMYGH
HHHHCCCHHHHCCC
>Mature Secondary Structure
MDFPSRLLLLLEVAELGSFSKVAEQRNVDRSVISKQINRLEHELGVHLLNRTTRSLSLTA
CCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHEEEHH
VGHEMVSQAKSLRTLLNDSHRLAQYYHSEPKGVLRITSSTLFGRQHVQQAVLVFQQQYPD
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCC
VDIELRLEDRLIDMVGEGFDIGFRIGKPKDSNLITRKIARNRLLIVAAPEFIKKHGEIKT
CCEEEEHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCEEEEECHHHHHHCCCHHH
LEQLEALPAAVYAAPGLLIDKFSYLDANNETKQLQLNVVYKVNDVEMITQTAVAGNMLAV
HHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHH
VAAQMIDNEILEQKLIPVMTQLHLNDLGTFYAVYPHRDAPIKTKLFINTLKAIIGEEVPI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCC
WEKKIPNFDKMYGH
HHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]