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The map label for this gene is eptA [H]

Identifier: 167624779

GI number: 167624779

Start: 3482397

End: 3484037

Strand: Reverse

Name: eptA [H]

Synonym: Shal_2864

Alternate gene names: 167624779

Gene position: 3484037-3482397 (Counterclockwise)

Preceding gene: 167624780

Following gene: 167624776

Centisome position: 66.66

GC content: 37.05

Gene sequence:

>1641_bases
ATGATGAAACGCCCCTTTAAGTCTGGCGTATCCTATGCGCTTTTCACCTTAATACTCGCAATCTACTTCTCATTGGTTGT
GAACATTCCTATCTATGCCGAGTTGTACACAATATTTAATAATATTAATCCTGTTAAAATTGGCTTTGTACTATCAATTC
CAATATTTTTTGGGGCAGCATTAAATTTTCTTTTTAATTTATTTAGTTGGCCTGTTATAGCGAAACCTTTTTTTATCATC
TTGCTAATTCTTTCCAGCATTGTGAGTTATGCCGGTTATAATTATGGAACACTCTTTGATTACAATATGATCGCTAATAT
TGTGGAAACGGATTCGAGTGAAGCGGGGTCTTATCTAAGTAGTTATTCTGTTCTGTGGGTTGTGTTAATGGGCATCATAC
CCGCATTGATTGTGTTCATGGTCCCCATAGGGATCAAACAGAGTCCGGCTAAAATGCTTTCGATGAAGCTGGTTTCGATG
GCGCTATCATTGGTTGTAATAGTGGTGATCGCAGCCCTATATTATCAAGACTACTCATCGGTTGGTCGTAACAATCGCTA
TTTGAAAAAAGCCATTATTCCCACTCAGTTTGTCTATAGCACTACCGAGTACATCTGGAAAACCTTTCTTTCAGTTCCAC
TTGAATATCGCCAAATTGGATTGGATGCCAAACAGACAGATTCAACTCTCGCAGCATCCGAAAATAAACCAACGTTAATG
GTACTTGTTGTAGGTGAAACAGCACGTTCACAAAACTATGAATTAAATGGTTATCCTAGAGATACCAATGAATACACCCG
CGATCTCGGTATCATTTCATTTCAAAACGTAAGTTCATGCGGCACGGCCACCGCAGTCTCAGTTCCCTGTATGTTTTCAT
CGATGACGAAAAATGAATTCAATCGAGATGTTGCTGATAATCAAGATAATGTATTGGATATCTTAAAACGCGCTAATATT
GCATTACTGTGGAAAGAAAATGATGGCGGAGATAAGAATGTTGCTGCTCGCATCGATAAAGTAACCATTGAACGTTCGAA
TAAAAATGAGTTTTGCGATGGTATGACATGCTATGACATGGCTTTACTGGACAACCTTGAGCAAGATATTGATTCCCTTG
AAGGGAATAGAATGATCATTCTTCATATTATGGGAAGCCATGGGCCGACATATTATAAGCGCTACCCAGCAGAGATGAAA
AAATTTAAACCAGACTGTCCCAGAAGTGATATAGAAAATTGCTCCATAGAAGAGATTGTTAATTCATACGATAATACAAT
TTTATATACCGACTATGTGGTCAGCAATGTTATCTCAAAGTTAGAGAAACTTGAAGATAAGTACAATACATCACTTATTT
ACATATCTGACCATGGTGAATCTCTTGGTGAGAAAGGCATGTTTTTGCATGGTATGCCTTATAGTTTCGCCCCTGATTAC
CAAACAAAAGTACCACTAATGGTATGGATGTCAGAAGGCTTTAAAGAAACGAAAAACATTAATAGTGAGTGTATTAAAAA
AGAAGCCGAATATAATCAATATTCACATGACAATATCTTTCATTCTATATTAGGCGTGATGGACGTTGAAACGAGTGATT
ATAAAAACAGTTTAGACATATTTAGTTCTTGTAAAGTGTAG

Upstream 100 bases:

>100_bases
TTGTTTGACTGTTTAGTCAAAATAGGGATGATGTCTGCTGGGAAATAGCTATCACTGCTGGAAAACTCTTTTGTATATAG
AATCTAATAATGTGAGTCAT

Downstream 100 bases:

>100_bases
ATAAAGTTAATAACCAGAATAAAAAATCCAGACGAGCATGGATGCTTAACAGGCTCTTAGATTTGGATATCGTTGCAAGC
CTGCCTGACCTCCATGGATG

Product: sulfatase

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MMKRPFKSGVSYALFTLILAIYFSLVVNIPIYAELYTIFNNINPVKIGFVLSIPIFFGAALNFLFNLFSWPVIAKPFFII
LLILSSIVSYAGYNYGTLFDYNMIANIVETDSSEAGSYLSSYSVLWVVLMGIIPALIVFMVPIGIKQSPAKMLSMKLVSM
ALSLVVIVVIAALYYQDYSSVGRNNRYLKKAIIPTQFVYSTTEYIWKTFLSVPLEYRQIGLDAKQTDSTLAASENKPTLM
VLVVGETARSQNYELNGYPRDTNEYTRDLGIISFQNVSSCGTATAVSVPCMFSSMTKNEFNRDVADNQDNVLDILKRANI
ALLWKENDGGDKNVAARIDKVTIERSNKNEFCDGMTCYDMALLDNLEQDIDSLEGNRMIILHIMGSHGPTYYKRYPAEMK
KFKPDCPRSDIENCSIEEIVNSYDNTILYTDYVVSNVISKLEKLEDKYNTSLIYISDHGESLGEKGMFLHGMPYSFAPDY
QTKVPLMVWMSEGFKETKNINSECIKKEAEYNQYSHDNIFHSILGVMDVETSDYKNSLDIFSSCKV

Sequences:

>Translated_546_residues
MMKRPFKSGVSYALFTLILAIYFSLVVNIPIYAELYTIFNNINPVKIGFVLSIPIFFGAALNFLFNLFSWPVIAKPFFII
LLILSSIVSYAGYNYGTLFDYNMIANIVETDSSEAGSYLSSYSVLWVVLMGIIPALIVFMVPIGIKQSPAKMLSMKLVSM
ALSLVVIVVIAALYYQDYSSVGRNNRYLKKAIIPTQFVYSTTEYIWKTFLSVPLEYRQIGLDAKQTDSTLAASENKPTLM
VLVVGETARSQNYELNGYPRDTNEYTRDLGIISFQNVSSCGTATAVSVPCMFSSMTKNEFNRDVADNQDNVLDILKRANI
ALLWKENDGGDKNVAARIDKVTIERSNKNEFCDGMTCYDMALLDNLEQDIDSLEGNRMIILHIMGSHGPTYYKRYPAEMK
KFKPDCPRSDIENCSIEEIVNSYDNTILYTDYVVSNVISKLEKLEDKYNTSLIYISDHGESLGEKGMFLHGMPYSFAPDY
QTKVPLMVWMSEGFKETKNINSECIKKEAEYNQYSHDNIFHSILGVMDVETSDYKNSLDIFSSCKV
>Mature_546_residues
MMKRPFKSGVSYALFTLILAIYFSLVVNIPIYAELYTIFNNINPVKIGFVLSIPIFFGAALNFLFNLFSWPVIAKPFFII
LLILSSIVSYAGYNYGTLFDYNMIANIVETDSSEAGSYLSSYSVLWVVLMGIIPALIVFMVPIGIKQSPAKMLSMKLVSM
ALSLVVIVVIAALYYQDYSSVGRNNRYLKKAIIPTQFVYSTTEYIWKTFLSVPLEYRQIGLDAKQTDSTLAASENKPTLM
VLVVGETARSQNYELNGYPRDTNEYTRDLGIISFQNVSSCGTATAVSVPCMFSSMTKNEFNRDVADNQDNVLDILKRANI
ALLWKENDGGDKNVAARIDKVTIERSNKNEFCDGMTCYDMALLDNLEQDIDSLEGNRMIILHIMGSHGPTYYKRYPAEMK
KFKPDCPRSDIENCSIEEIVNSYDNTILYTDYVVSNVISKLEKLEDKYNTSLIYISDHGESLGEKGMFLHGMPYSFAPDY
QTKVPLMVWMSEGFKETKNINSECIKKEAEYNQYSHDNIFHSILGVMDVETSDYKNSLDIFSSCKV

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=525, Percent_Identity=37.5238095238095, Blast_Score=400, Evalue=1e-113,
Organism=Escherichia coli, GI87082286, Length=558, Percent_Identity=25.4480286738351, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1790392, Length=427, Percent_Identity=24.3559718969555, Blast_Score=96, Evalue=8e-21,
Organism=Escherichia coli, GI87082223, Length=458, Percent_Identity=24.0174672489083, Blast_Score=96, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 61752; Mature: 61752

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKRPFKSGVSYALFTLILAIYFSLVVNIPIYAELYTIFNNINPVKIGFVLSIPIFFGAA
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEHHHHHHHHHHHH
LNFLFNLFSWPVIAKPFFIILLILSSIVSYAGYNYGTLFDYNMIANIVETDSSEAGSYLS
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHCCCCHHHHHHH
SYSVLWVVLMGIIPALIVFMVPIGIKQSPAKMLSMKLVSMALSLVVIVVIAALYYQDYSS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGRNNRYLKKAIIPTQFVYSTTEYIWKTFLSVPLEYRQIGLDAKQTDSTLAASENKPTLM
HCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHCCCHHCCCCCCCEEE
VLVVGETARSQNYELNGYPRDTNEYTRDLGIISFQNVSSCGTATAVSVPCMFSSMTKNEF
EEEECCCCCCCCCCCCCCCCCCHHHHHHCCCEEECCCCCCCCCHHEEHHHHHHHHHHHHH
NRDVADNQDNVLDILKRANIALLWKENDGGDKNVAARIDKVTIERSNKNEFCDGMTCYDM
CCCCCCCCHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHEEEECCCCCCCCCCCHHHHH
ALLDNLEQDIDSLEGNRMIILHIMGSHGPTYYKRYPAEMKKFKPDCPRSDIENCSIEEIV
HHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHCCHHHHHCCCCCCCCCCCCCCHHHHH
NSYDNTILYTDYVVSNVISKLEKLEDKYNTSLIYISDHGESLGEKGMFLHGMPYSFAPDY
HHHCCEEEEHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCEEECCCCCCCCCC
QTKVPLMVWMSEGFKETKNINSECIKKEAEYNQYSHDNIFHSILGVMDVETSDYKNSLDI
CCCCCEEEEEHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHH
FSSCKV
HHCCCC
>Mature Secondary Structure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HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8391535; 11677609 [H]