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The map label for this gene is mdeA [H]

Identifier: 167624669

GI number: 167624669

Start: 3339181

End: 3340377

Strand: Reverse

Name: mdeA [H]

Synonym: Shal_2751

Alternate gene names: 167624669

Gene position: 3340377-3339181 (Counterclockwise)

Preceding gene: 167624670

Following gene: 167624667

Centisome position: 63.91

GC content: 46.28

Gene sequence:

>1197_bases
ATGCAAGATAGATGGAAACTCGCCACAAAAGTGATTCATGCCGGACGGGAAAGTAATCAAGCTGGTGCACTAGTGACGCC
ATTATGCCAAAGCGCCACTTTTGTGTTTGATAGCGCAGAGCAGGGCGGAGCGAGATTTGCAGGAACTGAGCAAGGTTATA
TTTATACTCGTTTAGGAAACCCGACTACAGCAGAGCTTGAGCGAAAAATTGCCGCACTTGAAGGCAGTGAAGCTGCAGCC
GCTACAGCCTCAGGTATGGCTGCGGTATCGGCGGCGTTGTTAGCCAATTTAAGCCATGGCGACCACTTAGTGGCATCCAA
TGCCGTATATGGCTGCACCTTCTCACTGATGACCTCACTGTTAACTAAATTCGGCATTGAAGTGACGTTAGTTAACTTCA
GTGATGAAGCCGCAATTAAAGCCGCGATAAAACCCAATACCAAAGTGATTTTTTGTGAAACACCGGTAAATCCCCATTTA
GATGTGTACAACCTGGATCTAATCGCCGCCATTGCTAAGCAACACAATTTAGTGAGTGTGGTGGATAACACCTTTATGAC
GCCGTTATTGCAGCAACCGATTAAGCATGGCATCGATATTGTTATTCATAGTGCAACTAAATACCTCAATGGTCACGGTG
ATGTTATCGCTGGGCTTGTATGCGCGAGTAATGAGCAGATGGAGGTGATTAAATATGAAGTACTAAAGGATGTTGGTGCA
GTACTGTCTCCCCATGATGCGTGGCTCATTCTTCGCGGGTTAAAAACCTTAGATGTTCGTCTTGAAAGGCACTGTGATAA
CGCCGAGAAAGTTGCTAGCTACCTTGAGTACCACCCTAAAGTATCTAAGGTGTACTACCCGACACTTAAAAGCCATCCTG
GCCATAAATTGCTTGGCCCGCAGATGCGAAGAGCGGGCGGTGTAATAGCGTTTGAGCTTGACGCAAGCCTTGAAGAATCA
ATAAAGTTTATTAATCAGTTACCGTTATTTTCTATTGCGGTCAGTCTTGGCGATGCCGAGTCGCTGATCCAGCACCCCGC
CTCAATGACACATTCTCCTTATACAGATGAGGAGCGGAGCAGAGCTGGGATCACTGACAATTTACTGAGGATCTCGGTGG
GCCTTGAATCCGTAGAAGATATCATTCAAGCACTTGAGGGGGGCTTAGATAAGTTGCACATTAACCAATTGCTATAA

Upstream 100 bases:

>100_bases
TGTTGATGAAATGGCTTGGCAAATTCATTTGAGATTTAAAAGATAAAGCTGACATTCACAACTATTAAAAAGTCAGTGTA
GATCAAGTAGGGAGTGCAAT

Downstream 100 bases:

>100_bases
AACTCGAATGGTTCAATAAAGTATAAAAAGAGCCCGACCTAAGGTTGGGCTCTTCTGTATTAGGTTAAATATCCCAGAAA
GTATGGGCGATGATATAAAG

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MQDRWKLATKVIHAGRESNQAGALVTPLCQSATFVFDSAEQGGARFAGTEQGYIYTRLGNPTTAELERKIAALEGSEAAA
ATASGMAAVSAALLANLSHGDHLVASNAVYGCTFSLMTSLLTKFGIEVTLVNFSDEAAIKAAIKPNTKVIFCETPVNPHL
DVYNLDLIAAIAKQHNLVSVVDNTFMTPLLQQPIKHGIDIVIHSATKYLNGHGDVIAGLVCASNEQMEVIKYEVLKDVGA
VLSPHDAWLILRGLKTLDVRLERHCDNAEKVASYLEYHPKVSKVYYPTLKSHPGHKLLGPQMRRAGGVIAFELDASLEES
IKFINQLPLFSIAVSLGDAESLIQHPASMTHSPYTDEERSRAGITDNLLRISVGLESVEDIIQALEGGLDKLHINQLL

Sequences:

>Translated_398_residues
MQDRWKLATKVIHAGRESNQAGALVTPLCQSATFVFDSAEQGGARFAGTEQGYIYTRLGNPTTAELERKIAALEGSEAAA
ATASGMAAVSAALLANLSHGDHLVASNAVYGCTFSLMTSLLTKFGIEVTLVNFSDEAAIKAAIKPNTKVIFCETPVNPHL
DVYNLDLIAAIAKQHNLVSVVDNTFMTPLLQQPIKHGIDIVIHSATKYLNGHGDVIAGLVCASNEQMEVIKYEVLKDVGA
VLSPHDAWLILRGLKTLDVRLERHCDNAEKVASYLEYHPKVSKVYYPTLKSHPGHKLLGPQMRRAGGVIAFELDASLEES
IKFINQLPLFSIAVSLGDAESLIQHPASMTHSPYTDEERSRAGITDNLLRISVGLESVEDIIQALEGGLDKLHINQLL
>Mature_398_residues
MQDRWKLATKVIHAGRESNQAGALVTPLCQSATFVFDSAEQGGARFAGTEQGYIYTRLGNPTTAELERKIAALEGSEAAA
ATASGMAAVSAALLANLSHGDHLVASNAVYGCTFSLMTSLLTKFGIEVTLVNFSDEAAIKAAIKPNTKVIFCETPVNPHL
DVYNLDLIAAIAKQHNLVSVVDNTFMTPLLQQPIKHGIDIVIHSATKYLNGHGDVIAGLVCASNEQMEVIKYEVLKDVGA
VLSPHDAWLILRGLKTLDVRLERHCDNAEKVASYLEYHPKVSKVYYPTLKSHPGHKLLGPQMRRAGGVIAFELDASLEES
IKFINQLPLFSIAVSLGDAESLIQHPASMTHSPYTDEERSRAGITDNLLRISVGLESVEDIIQALEGGLDKLHINQLL

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=371, Percent_Identity=40.4312668463612, Blast_Score=265, Evalue=4e-71,
Organism=Homo sapiens, GI299473759, Length=371, Percent_Identity=37.466307277628, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI25453487, Length=370, Percent_Identity=35.6756756756757, Blast_Score=209, Evalue=4e-54,
Organism=Escherichia coli, GI1790375, Length=385, Percent_Identity=38.4415584415584, Blast_Score=265, Evalue=4e-72,
Organism=Escherichia coli, GI1789383, Length=403, Percent_Identity=30.0248138957816, Blast_Score=182, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17560056, Length=375, Percent_Identity=41.0666666666667, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17538055, Length=375, Percent_Identity=41.6, Blast_Score=276, Evalue=9e-75,
Organism=Caenorhabditis elegans, GI71980708, Length=385, Percent_Identity=40.7792207792208, Blast_Score=275, Evalue=3e-74,
Organism=Saccharomyces cerevisiae, GI6319307, Length=404, Percent_Identity=38.3663366336634, Blast_Score=261, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6323333, Length=433, Percent_Identity=31.6397228637413, Blast_Score=201, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6321254, Length=404, Percent_Identity=31.4356435643564, Blast_Score=172, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6321170, Length=336, Percent_Identity=32.1428571428571, Blast_Score=163, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6321904, Length=411, Percent_Identity=28.2238442822384, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI19922554, Length=392, Percent_Identity=40.3061224489796, Blast_Score=275, Evalue=3e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 43055; Mature: 43055

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDRWKLATKVIHAGRESNQAGALVTPLCQSATFVFDSAEQGGARFAGTEQGYIYTRLGN
CCCHHHHHHHHHHCCCCCCCCCCCHHHHHCCCEEEEECCCCCCCCCCCCCCCEEEEECCC
PTTAELERKIAALEGSEAAAATASGMAAVSAALLANLSHGDHLVASNAVYGCTFSLMTSL
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHH
LTKFGIEVTLVNFSDEAAIKAAIKPNTKVIFCETPVNPHLDVYNLDLIAAIAKQHNLVSV
HHHCCCEEEEEECCCCCEEEEEECCCCEEEEEECCCCCCCEEECHHHHHHHHHCCCHHHH
VDNTFMTPLLQQPIKHGIDIVIHSATKYLNGHGDVIAGLVCASNEQMEVIKYEVLKDVGA
HHHHHHHHHHHHHHHCCCEEEEECHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
VLSPHDAWLILRGLKTLDVRLERHCDNAEKVASYLEYHPKVSKVYYPTLKSHPGHKLLGP
HCCCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCH
QMRRAGGVIAFELDASLEESIKFINQLPLFSIAVSLGDAESLIQHPASMTHSPYTDEERS
HHHHHCCEEEEEECCCHHHHHHHHHHCCHHHHHHHCCCHHHHHHCCHHCCCCCCCCCHHH
RAGITDNLLRISVGLESVEDIIQALEGGLDKLHINQLL
HCCCCCCCEEEEECHHHHHHHHHHHHCCCCCHHHHHCC
>Mature Secondary Structure
MQDRWKLATKVIHAGRESNQAGALVTPLCQSATFVFDSAEQGGARFAGTEQGYIYTRLGN
CCCHHHHHHHHHHCCCCCCCCCCCHHHHHCCCEEEEECCCCCCCCCCCCCCCEEEEECCC
PTTAELERKIAALEGSEAAAATASGMAAVSAALLANLSHGDHLVASNAVYGCTFSLMTSL
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHH
LTKFGIEVTLVNFSDEAAIKAAIKPNTKVIFCETPVNPHLDVYNLDLIAAIAKQHNLVSV
HHHCCCEEEEEECCCCCEEEEEECCCCEEEEEECCCCCCCEEECHHHHHHHHHCCCHHHH
VDNTFMTPLLQQPIKHGIDIVIHSATKYLNGHGDVIAGLVCASNEQMEVIKYEVLKDVGA
HHHHHHHHHHHHHHHCCCEEEEECHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
VLSPHDAWLILRGLKTLDVRLERHCDNAEKVASYLEYHPKVSKVYYPTLKSHPGHKLLGP
HCCCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCH
QMRRAGGVIAFELDASLEESIKFINQLPLFSIAVSLGDAESLIQHPASMTHSPYTDEERS
HHHHHCCEEEEEECCCHHHHHHHHHHCCHHHHHHHCCCHHHHHHCCHHCCCCCCCCCHHH
RAGITDNLLRISVGLESVEDIIQALEGGLDKLHINQLL
HCCCCCCCEEEEECHHHHHHHHHHHHCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]