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The map label for this gene is polB [H]

Identifier: 167624647

GI number: 167624647

Start: 3310011

End: 3312452

Strand: Reverse

Name: polB [H]

Synonym: Shal_2729

Alternate gene names: 167624647

Gene position: 3312452-3310011 (Counterclockwise)

Preceding gene: 167624653

Following gene: 167624646

Centisome position: 63.37

GC content: 44.8

Gene sequence:

>2442_bases
ATGAATTCAAGCTTGAGCGAACAGCAAGTGATTAAAGGTCGAGTCTTGACTCGCCATGCGGTCAATCGGCAGGGCAGCCT
GATCTTGCAGTATTTTGTTCAAACCGAAGCGGGGCCAGTGACTGTTGAAATTCCTCATCAAGAGCATGTTTGCTTTATCC
ATACCAGTGATGAATTCCGCTTATTACAAAGCACAGACCTAAGAGGGGTCCGCTGTAGTACGCTAGCACTTAAGAGCTTT
CAAAATATTCCTGTATCAGGGCTTTATTGTGGCGATGCACGCCAGTTACGCACGTTAGTGAGAGCCGCAAGAGATCTGGA
TATTGAGCTGTTTGAATCAGACGTAAAAACCGAGCATAGATTCTTGATTGAACGCTTAATCGCATTAGATGCGGAGTTTC
AGGGGCAGTTTCAAGAGCCGTCCACTCAAAGCCCACAAGTCATAGCCCCGGTCTCAGATCTAGCCCCAGCGAAGCGTCAG
CCACTGTTTATTGCTAATAGGGCGCGTAGAACTGAATCGACGATAAGGCTTAGAGCCATCTCTTTAGACTTTGAGTGTAG
CTTTAGCGGCGAACTCTACTCTGTTGGCCTGTATGGACAAACACTTGACGGTATTGTCTACCAAAAAGTGATTATGGTCG
GTGAGCCCGAGGAGACGGAGCAAGGCTACATCGAATGGGTTGCCAATGAGTTCGAGCTCATCATGCGACTAATAGCATGG
TTTAATGAATATGATCCCGACATTATCATTGGTTGGTCAGTGGTCACTTTCGACTTGGCGTTACTTTATCGCCGAGCGAA
ATACCATAACATCGCCCTCAATATCGGTCGTGATAATCATCCTCTTAGCTGGAAAGTCGAAGATAAGTTTCGCCCAGAAA
CATTGTCGCTCCCAGGCCGAGTCGTATTAGATGGTATTGATTGGCTTAAGGCGGCCTTTTATCAATTTGAGCGCTACTCG
CTCGAATTTGTGTCACAAGTGCTATTGGATGAAGGTAAAGCGATTCATAACGTTGATAACCGCATGGATGAAATCAACAG
TCTGTTTTTGAATGACAAACCGGCCCTAGCTCACTACAACCTTACCGATAGCCGCTTAGTTTGGGATATTTTTGAAAAGA
CCCAATTATTTGATTTTGTCATTGAGCGCGCCAAGCTGACCGGTCTTGAGCTTGGCCGTGTTGGCGCCTCGGTGGCGGCG
TTCAATAATTTGTACTTGCCTCACCTACATCGAGCGGGTTTTGTTGCGCCAAGTAAACCCGTTAGTGATGGCATAGAGAG
TCCGGGTGGGTATGTGATGGACTCGGTTCCGGGCCTGTACCGCCATATTTTAGTGCTTGATTTTAAAAGTCTATACCCCT
CAATTATTCGAACTTTCCTTATTGATCCTAAGGGCTTAGTTCAAGGACTTAATCAATTGCTTAATCAAGGGCAAGACGAA
ACTGTACCCGGTTTTCTCGGTGCTAGGTTTAGCCGTGAAAAGCCGATACTCCCCATGTTAATCAAAAAATTATCAGAGGA
GAGGGAGTCCGCCAAGAAACACCTGGATGCGCCGCTATCGCAAGCAATAAAAATCATCATGAACTCGTTATATGGCGTAC
TGGGATCCCGCGGCTGTGTGTTTCACGACGCTCGCTTAGCGAGTTCGATCACCATGCGTGGCCATGAAATCATGAAACAA
ACAAGGCGTTGGATCGAATCTGAAGGCTATGAGGTCATCTACGGTGATACCGATTCCACCTTTGTTTGGCTTGGCAATGA
CTTTGATCCCGCTAAGGTGCTGCAAACCGGTAAAGCACTGGCTAAAAAGGTCAATCAGTTATGGGGTGACAAGCTTAGTA
ATGAGTTCGATCTAGAGAGCTTTCTGGAATTGGAGTTTGAGACCCATTTTGAACAGTTTTTTATGCCTACACTAAGAGGC
TCTATAGAAGGCTCAAAGAAACGCTATGTTGGAACTAAACGTAATAATCTGGGGGACTCTGAATTAATTTTTAAAGGCAT
GGAGCAAGTTCGTAGCGATTGGTCGCCGCTCGCAAGGCGAATTCAATATGAGCTTTATGACAGATTGTTCCAACAACAAG
ACGTTGAGTCTTACTTAAGAGAAATCGTAGATAAGCTAAATCGTGGTGATCTTGATAATGAACTGGTTTTTACTAAGCAG
TTAAGGCGTGATCTAGAGGAATATACCGCTAAGTCTGCCCCGCATTTAAAAGCCGCCCGCAAACTCTGTGATGCAACGGG
GGACTCTCAGTACGGTAAACGCGGTGCTCGAATTGAGTATGTGATGACATTAAATGGTGCTGAACCTGTACTATATACTT
CTGCATTAATTGATTATAACTATTATCTTTCTCGGCAATTAGAACCGATTGCTGAACCAATCCTTACAATATTGAAAACA
ACTTTCAACAAAGTTACTACAAATCAAATGTCGTTAATTTAA

Upstream 100 bases:

>100_bases
GAATGCACTAAGCTAATAAATAGTGCTGTACATAATAACAGTGATTACTTAGCCTAAGAAGCGTTTAGTGATTAATAGTG
CATTTAATTGAGTATAAAGT

Downstream 100 bases:

>100_bases
GGTGTTCATCATTAATCGATCGCTAATTAAATGTTAATGTTTGGTTCAAAGTCTTGGTGTAAGTGTATTTACCAGTTAAT
CCTGTGCAAAATCTAACGAA

Product: DNA polymerase II

Products: NA

Alternate protein names: Pol II [H]

Number of amino acids: Translated: 813; Mature: 813

Protein sequence:

>813_residues
MNSSLSEQQVIKGRVLTRHAVNRQGSLILQYFVQTEAGPVTVEIPHQEHVCFIHTSDEFRLLQSTDLRGVRCSTLALKSF
QNIPVSGLYCGDARQLRTLVRAARDLDIELFESDVKTEHRFLIERLIALDAEFQGQFQEPSTQSPQVIAPVSDLAPAKRQ
PLFIANRARRTESTIRLRAISLDFECSFSGELYSVGLYGQTLDGIVYQKVIMVGEPEETEQGYIEWVANEFELIMRLIAW
FNEYDPDIIIGWSVVTFDLALLYRRAKYHNIALNIGRDNHPLSWKVEDKFRPETLSLPGRVVLDGIDWLKAAFYQFERYS
LEFVSQVLLDEGKAIHNVDNRMDEINSLFLNDKPALAHYNLTDSRLVWDIFEKTQLFDFVIERAKLTGLELGRVGASVAA
FNNLYLPHLHRAGFVAPSKPVSDGIESPGGYVMDSVPGLYRHILVLDFKSLYPSIIRTFLIDPKGLVQGLNQLLNQGQDE
TVPGFLGARFSREKPILPMLIKKLSEERESAKKHLDAPLSQAIKIIMNSLYGVLGSRGCVFHDARLASSITMRGHEIMKQ
TRRWIESEGYEVIYGDTDSTFVWLGNDFDPAKVLQTGKALAKKVNQLWGDKLSNEFDLESFLELEFETHFEQFFMPTLRG
SIEGSKKRYVGTKRNNLGDSELIFKGMEQVRSDWSPLARRIQYELYDRLFQQQDVESYLREIVDKLNRGDLDNELVFTKQ
LRRDLEEYTAKSAPHLKAARKLCDATGDSQYGKRGARIEYVMTLNGAEPVLYTSALIDYNYYLSRQLEPIAEPILTILKT
TFNKVTTNQMSLI

Sequences:

>Translated_813_residues
MNSSLSEQQVIKGRVLTRHAVNRQGSLILQYFVQTEAGPVTVEIPHQEHVCFIHTSDEFRLLQSTDLRGVRCSTLALKSF
QNIPVSGLYCGDARQLRTLVRAARDLDIELFESDVKTEHRFLIERLIALDAEFQGQFQEPSTQSPQVIAPVSDLAPAKRQ
PLFIANRARRTESTIRLRAISLDFECSFSGELYSVGLYGQTLDGIVYQKVIMVGEPEETEQGYIEWVANEFELIMRLIAW
FNEYDPDIIIGWSVVTFDLALLYRRAKYHNIALNIGRDNHPLSWKVEDKFRPETLSLPGRVVLDGIDWLKAAFYQFERYS
LEFVSQVLLDEGKAIHNVDNRMDEINSLFLNDKPALAHYNLTDSRLVWDIFEKTQLFDFVIERAKLTGLELGRVGASVAA
FNNLYLPHLHRAGFVAPSKPVSDGIESPGGYVMDSVPGLYRHILVLDFKSLYPSIIRTFLIDPKGLVQGLNQLLNQGQDE
TVPGFLGARFSREKPILPMLIKKLSEERESAKKHLDAPLSQAIKIIMNSLYGVLGSRGCVFHDARLASSITMRGHEIMKQ
TRRWIESEGYEVIYGDTDSTFVWLGNDFDPAKVLQTGKALAKKVNQLWGDKLSNEFDLESFLELEFETHFEQFFMPTLRG
SIEGSKKRYVGTKRNNLGDSELIFKGMEQVRSDWSPLARRIQYELYDRLFQQQDVESYLREIVDKLNRGDLDNELVFTKQ
LRRDLEEYTAKSAPHLKAARKLCDATGDSQYGKRGARIEYVMTLNGAEPVLYTSALIDYNYYLSRQLEPIAEPILTILKT
TFNKVTTNQMSLI
>Mature_813_residues
MNSSLSEQQVIKGRVLTRHAVNRQGSLILQYFVQTEAGPVTVEIPHQEHVCFIHTSDEFRLLQSTDLRGVRCSTLALKSF
QNIPVSGLYCGDARQLRTLVRAARDLDIELFESDVKTEHRFLIERLIALDAEFQGQFQEPSTQSPQVIAPVSDLAPAKRQ
PLFIANRARRTESTIRLRAISLDFECSFSGELYSVGLYGQTLDGIVYQKVIMVGEPEETEQGYIEWVANEFELIMRLIAW
FNEYDPDIIIGWSVVTFDLALLYRRAKYHNIALNIGRDNHPLSWKVEDKFRPETLSLPGRVVLDGIDWLKAAFYQFERYS
LEFVSQVLLDEGKAIHNVDNRMDEINSLFLNDKPALAHYNLTDSRLVWDIFEKTQLFDFVIERAKLTGLELGRVGASVAA
FNNLYLPHLHRAGFVAPSKPVSDGIESPGGYVMDSVPGLYRHILVLDFKSLYPSIIRTFLIDPKGLVQGLNQLLNQGQDE
TVPGFLGARFSREKPILPMLIKKLSEERESAKKHLDAPLSQAIKIIMNSLYGVLGSRGCVFHDARLASSITMRGHEIMKQ
TRRWIESEGYEVIYGDTDSTFVWLGNDFDPAKVLQTGKALAKKVNQLWGDKLSNEFDLESFLELEFETHFEQFFMPTLRG
SIEGSKKRYVGTKRNNLGDSELIFKGMEQVRSDWSPLARRIQYELYDRLFQQQDVESYLREIVDKLNRGDLDNELVFTKQ
LRRDLEEYTAKSAPHLKAARKLCDATGDSQYGKRGARIEYVMTLNGAEPVLYTSALIDYNYYLSRQLEPIAEPILTILKT
TFNKVTTNQMSLI

Specific function: Thought to be involved in DNA repair and/or mutagenesis [H]

COG id: COG0417

COG function: function code L; DNA polymerase elongation subunit (family B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase type-B family [H]

Homologues:

Organism=Homo sapiens, GI156616275, Length=622, Percent_Identity=27.8135048231511, Blast_Score=138, Evalue=2e-32,
Organism=Homo sapiens, GI106507301, Length=653, Percent_Identity=26.0336906584992, Blast_Score=130, Evalue=7e-30,
Organism=Escherichia coli, GI1786246, Length=816, Percent_Identity=44.4852941176471, Blast_Score=649, Evalue=0.0,
Organism=Caenorhabditis elegans, GI32565317, Length=663, Percent_Identity=26.0935143288084, Blast_Score=140, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17559488, Length=709, Percent_Identity=25.1057827926657, Blast_Score=129, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6320101, Length=648, Percent_Identity=25.7716049382716, Blast_Score=120, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6324227, Length=601, Percent_Identity=24.126455906822, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648780, Length=411, Percent_Identity=27.7372262773723, Blast_Score=134, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24664937, Length=637, Percent_Identity=28.5714285714286, Blast_Score=130, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006172
- InterPro:   IPR017964
- InterPro:   IPR006133
- InterPro:   IPR006134
- InterPro:   IPR023211
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00136 DNA_pol_B; PF03104 DNA_pol_B_exo [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 92866; Mature: 92866

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00116 DNA_POLYMERASE_B

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSLSEQQVIKGRVLTRHAVNRQGSLILQYFVQTEAGPVTVEIPHQEHVCFIHTSDEFR
CCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEECCCCCEEEEECCCCCE
LLQSTDLRGVRCSTLALKSFQNIPVSGLYCGDARQLRTLVRAARDLDIELFESDVKTEHR
EHHCCCCCCCCHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
FLIERLIALDAEFQGQFQEPSTQSPQVIAPVSDLAPAKRQPLFIANRARRTESTIRLRAI
HHHHHHHHHCCHHCCCCCCCCCCCCEEEECHHHCCCCCCCCEEEEHHHHCCCCEEEEEEE
SLDFECSFSGELYSVGLYGQTLDGIVYQKVIMVGEPEETEQGYIEWVANEFELIMRLIAW
EEEEEECCCCCEEEEEECCCHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHH
FNEYDPDIIIGWSVVTFDLALLYRRAKYHNIALNIGRDNHPLSWKVEDKFRPETLSLPGR
HCCCCCCEEEEHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCEEEECCCCCCCCCCCCCE
VVLDGIDWLKAAFYQFERYSLEFVSQVLLDEGKAIHNVDNRMDEINSLFLNDKPALAHYN
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEC
LTDSRLVWDIFEKTQLFDFVIERAKLTGLELGRVGASVAAFNNLYLPHLHRAGFVAPSKP
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHCCCCCCHHHCCCCCCCCC
VSDGIESPGGYVMDSVPGLYRHILVLDFKSLYPSIIRTFLIDPKGLVQGLNQLLNQGQDE
HHHCCCCCCCEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
TVPGFLGARFSREKPILPMLIKKLSEERESAKKHLDAPLSQAIKIIMNSLYGVLGSRGCV
CCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCE
FHDARLASSITMRGHEIMKQTRRWIESEGYEVIYGDTDSTFVWLGNDFDPAKVLQTGKAL
EEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHH
AKKVNQLWGDKLSNEFDLESFLELEFETHFEQFFMPTLRGSIEGSKKRYVGTKRNNLGDS
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCH
ELIFKGMEQVRSDWSPLARRIQYELYDRLFQQQDVESYLREIVDKLNRGDLDNELVFTKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LRRDLEEYTAKSAPHLKAARKLCDATGDSQYGKRGARIEYVMTLNGAEPVLYTSALIDYN
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHH
YYLSRQLEPIAEPILTILKTTFNKVTTNQMSLI
HHHHHCCHHHHHHHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure
MNSSLSEQQVIKGRVLTRHAVNRQGSLILQYFVQTEAGPVTVEIPHQEHVCFIHTSDEFR
CCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEECCCCCEEEEECCCCCE
LLQSTDLRGVRCSTLALKSFQNIPVSGLYCGDARQLRTLVRAARDLDIELFESDVKTEHR
EHHCCCCCCCCHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
FLIERLIALDAEFQGQFQEPSTQSPQVIAPVSDLAPAKRQPLFIANRARRTESTIRLRAI
HHHHHHHHHCCHHCCCCCCCCCCCCEEEECHHHCCCCCCCCEEEEHHHHCCCCEEEEEEE
SLDFECSFSGELYSVGLYGQTLDGIVYQKVIMVGEPEETEQGYIEWVANEFELIMRLIAW
EEEEEECCCCCEEEEEECCCHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHH
FNEYDPDIIIGWSVVTFDLALLYRRAKYHNIALNIGRDNHPLSWKVEDKFRPETLSLPGR
HCCCCCCEEEEHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCEEEECCCCCCCCCCCCCE
VVLDGIDWLKAAFYQFERYSLEFVSQVLLDEGKAIHNVDNRMDEINSLFLNDKPALAHYN
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEC
LTDSRLVWDIFEKTQLFDFVIERAKLTGLELGRVGASVAAFNNLYLPHLHRAGFVAPSKP
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHCCCCCCHHHCCCCCCCCC
VSDGIESPGGYVMDSVPGLYRHILVLDFKSLYPSIIRTFLIDPKGLVQGLNQLLNQGQDE
HHHCCCCCCCEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
TVPGFLGARFSREKPILPMLIKKLSEERESAKKHLDAPLSQAIKIIMNSLYGVLGSRGCV
CCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCE
FHDARLASSITMRGHEIMKQTRRWIESEGYEVIYGDTDSTFVWLGNDFDPAKVLQTGKAL
EEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHH
AKKVNQLWGDKLSNEFDLESFLELEFETHFEQFFMPTLRGSIEGSKKRYVGTKRNNLGDS
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCH
ELIFKGMEQVRSDWSPLARRIQYELYDRLFQQQDVESYLREIVDKLNRGDLDNELVFTKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LRRDLEEYTAKSAPHLKAARKLCDATGDSQYGKRGARIEYVMTLNGAEPVLYTSALIDYN
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHH
YYLSRQLEPIAEPILTILKTTFNKVTTNQMSLI
HHHHHCCHHHHHHHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2034216; 2261080; 1630901; 9278503; 2217198; 9079692 [H]