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The map label for this gene is rlmI [H]

Identifier: 167624520

GI number: 167624520

Start: 3153548

End: 3154456

Strand: Reverse

Name: rlmI [H]

Synonym: Shal_2599

Alternate gene names: 167624520

Gene position: 3154456-3153548 (Counterclockwise)

Preceding gene: 167624521

Following gene: 167624518

Centisome position: 60.35

GC content: 42.79

Gene sequence:

>909_bases
ATGCAGTTATTTATTGAAGCAGTAAAGCAAATAGAGTTATCAGAAGATGTCGGACGGCTATTTCATGGTCGAGGAGGGCG
TTTTCAAGGCTGTGAACATCTTTGTCTCGATTGGTTTTCACCGGTGCTACTGTTAACCAGTTTTAAAGTGTTAGAAAGCG
ATGAGCTAGACAGCTTATGTGCAGCCATTGAGCAACGCTGGGTTGAGCTAAAGGGCGATGAGCCTCTTAATCTTGTGTTT
CAATACCGCCGTGGTGGTGAGACTCACACCGAGATCATTAAAGGTGAAGTACCAGAGAAGCACATAGTCACTGAAAATGG
TGCATGTTTTCAAGTGCATCTGTTGCGCGGTCAAAACCATGGTTTGTTTCTCGATATGCGCAATGGCCGTCAATGGGTAC
GTGAACACAGTGCACACAAGAAAGTATTAAATCTGTTTGCTTATACTTGTGGTTTCTCTATAGCTGCGCTGCAGGGAAAG
GCGGATGAAGTGGTTAACATCGATATGAGTAAAGGTGCATTGTCGATTGGTAAGCAAAATCATCTACTCAATGATTTCAA
AGTCGGCGCGCGTTTTCTGGGGCATGATATCTTTAAGTCATGGGGTAAGCTCACTAAACTTGGCCCATATGACTTGATCA
TCGCCGATCCGCCTAGTAATCAAAAGGGCAGTTTTGTTGCTACTAAAGATTATGCTCGTTTGTTAAAGCGACTACCAGAG
CTTTTGGCGGATGACGGTGACATGCTGCTTTGCTTAAACGCTCCAGAGTTAAGCATAGAGTTTCTAATGAATCAGGTAAA
AGAGTACAGCCCAAGTTTGGTATTTTGTGAGCAGCTAGAAAATCCTGCAGTTTTTGAAGATATCGATCCTAACAAAGCTC
TAAAAGTGCTACGTTATCAAAAATCTTAA

Upstream 100 bases:

>100_bases
CCGCCTCTGCTCAGGAAGAAAGCGCTGAGTTAGAAAACTCTCAGATAGAGAACTCTCCGTTAGAGAGTTCAGAGTTAAAG
AATAGAGAAAAGTGAGTTTG

Downstream 100 bases:

>100_bases
CCAGTAGATTGATATAAAAAAGGCCAAACCCAGTGTTTGGCCTTTTTTATGCGCCAACGTTAGTAAATGAGCCGCTTCAA
GCTAATATTAGGCCATACCC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MQLFIEAVKQIELSEDVGRLFHGRGGRFQGCEHLCLDWFSPVLLLTSFKVLESDELDSLCAAIEQRWVELKGDEPLNLVF
QYRRGGETHTEIIKGEVPEKHIVTENGACFQVHLLRGQNHGLFLDMRNGRQWVREHSAHKKVLNLFAYTCGFSIAALQGK
ADEVVNIDMSKGALSIGKQNHLLNDFKVGARFLGHDIFKSWGKLTKLGPYDLIIADPPSNQKGSFVATKDYARLLKRLPE
LLADDGDMLLCLNAPELSIEFLMNQVKEYSPSLVFCEQLENPAVFEDIDPNKALKVLRYQKS

Sequences:

>Translated_302_residues
MQLFIEAVKQIELSEDVGRLFHGRGGRFQGCEHLCLDWFSPVLLLTSFKVLESDELDSLCAAIEQRWVELKGDEPLNLVF
QYRRGGETHTEIIKGEVPEKHIVTENGACFQVHLLRGQNHGLFLDMRNGRQWVREHSAHKKVLNLFAYTCGFSIAALQGK
ADEVVNIDMSKGALSIGKQNHLLNDFKVGARFLGHDIFKSWGKLTKLGPYDLIIADPPSNQKGSFVATKDYARLLKRLPE
LLADDGDMLLCLNAPELSIEFLMNQVKEYSPSLVFCEQLENPAVFEDIDPNKALKVLRYQKS
>Mature_302_residues
MQLFIEAVKQIELSEDVGRLFHGRGGRFQGCEHLCLDWFSPVLLLTSFKVLESDELDSLCAAIEQRWVELKGDEPLNLVF
QYRRGGETHTEIIKGEVPEKHIVTENGACFQVHLLRGQNHGLFLDMRNGRQWVREHSAHKKVLNLFAYTCGFSIAALQGK
ADEVVNIDMSKGALSIGKQNHLLNDFKVGARFLGHDIFKSWGKLTKLGPYDLIIADPPSNQKGSFVATKDYARLLKRLPE
LLADDGDMLLCLNAPELSIEFLMNQVKEYSPSLVFCEQLENPAVFEDIDPNKALKVLRYQKS

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=248, Percent_Identity=27.4193548387097, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1787181, Length=192, Percent_Identity=31.7708333333333, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 34172; Mature: 34172

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLFIEAVKQIELSEDVGRLFHGRGGRFQGCEHLCLDWFSPVLLLTSFKVLESDELDSLC
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
AAIEQRWVELKGDEPLNLVFQYRRGGETHTEIIKGEVPEKHIVTENGACFQVHLLRGQNH
HHHHHHHEECCCCCCHHHHEEECCCCCHHHHHHCCCCCCCEEEECCCCEEEEEEEECCCC
GLFLDMRNGRQWVREHSAHKKVLNLFAYTCGFSIAALQGKADEVVNIDMSKGALSIGKQN
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEECCCCCHHCCCCC
HLLNDFKVGARFLGHDIFKSWGKLTKLGPYDLIIADPPSNQKGSFVATKDYARLLKRLPE
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHHH
LLADDGDMLLCLNAPELSIEFLMNQVKEYSPSLVFCEQLENPAVFEDIDPNKALKVLRYQ
HHCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEHHHCCCCCCCCCCCHHHHHHHHHHC
KS
CC
>Mature Secondary Structure
MQLFIEAVKQIELSEDVGRLFHGRGGRFQGCEHLCLDWFSPVLLLTSFKVLESDELDSLC
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
AAIEQRWVELKGDEPLNLVFQYRRGGETHTEIIKGEVPEKHIVTENGACFQVHLLRGQNH
HHHHHHHEECCCCCCHHHHEEECCCCCHHHHHHCCCCCCCEEEECCCCEEEEEEEECCCC
GLFLDMRNGRQWVREHSAHKKVLNLFAYTCGFSIAALQGKADEVVNIDMSKGALSIGKQN
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEECCCCCHHCCCCC
HLLNDFKVGARFLGHDIFKSWGKLTKLGPYDLIIADPPSNQKGSFVATKDYARLLKRLPE
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHHH
LLADDGDMLLCLNAPELSIEFLMNQVKEYSPSLVFCEQLENPAVFEDIDPNKALKVLRYQ
HHCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEHHHCCCCCCCCCCCHHHHHHHHHHC
KS
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA