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The map label for this gene is focA [H]

Identifier: 167624511

GI number: 167624511

Start: 3139804

End: 3141285

Strand: Reverse

Name: focA [H]

Synonym: Shal_2590

Alternate gene names: 167624511

Gene position: 3141285-3139804 (Counterclockwise)

Preceding gene: 167624515

Following gene: 167624509

Centisome position: 60.1

GC content: 44.2

Gene sequence:

>1482_bases
ATGTTTTCTAATCATGCGGTTAAAGCGCCGGTGATAGTTAACCCAATCCAACAAAGTGTTGAGCTTCCCAAGCCAGAATT
ATTGAGCCTGTATCAACAGGCTGAGCATTATGGCAAGGCTAAGGTGATTAAATCTGCATGGCAGTCGTTCGGTTTAGCGG
CCTTTGCTGGTGCTTTTATTGCGCTGGCGTTTGTTTTTTATATCACAGTGACAACTGGAAGCGATGGAGCATGGGGATTA
GTTCGCCTTGCTGGGGGATTAGCATTTAGTTTAGGTCTGATGCTAGTGGTGATCTGTGGCGGGGAGCTTTTTACCAGTAC
CGTTCTTAGTAGCGTTGCCTGGGCTCAGAAGCTTGTTACTACAATCGATCTAATTAAATGTTGGGGGCGAGTATACCTAG
GTAATCTAATTGGAGCGGTGATCATGTTGATTTTGATCATGTCAGCTCGAATGCACGAACTTGATGGGGGGCTGTGGGGA
TTAAACGCATTACAAATTGCCCAGCATAAGCTGCACCATAGTTGGGGGCAGGCCTTCGTTCTTGGCGTATTGTGCAATAT
GTTAGTTTGCTTAGGGGTGTGGATGACATTTTCCAGTCGTGACGCTCTCACCAAGGCGATCTTACTTATGCTGCCTGTTG
CCATGTTCGTTAGCAGCGGTTTTGAGCACAGCATTGCAAACCTGTTTATGGTTCCTTTAGGGATCACCATCCACCAGTTT
GCCGACCCAGCTTGGTTTGAAGCCTTAAATATCAGTCGTGATAGCTTTGCCGACCTGACTCTTAGCAATTTTATTTTCAA
TAATCTCATTCCCGTGACCTTAGGAAATATCGTCGGTGGTGGCGTTTTTGTTGGCTTAGGTTACTGGCTTATCGAAAAGT
CTGATCAAAGCCTAAAAACTTCCTTAGTTCATAGCACACCACAACTTGAAACATCTGCCCAAATACATCAAATCCCTACT
TCTGGAGTACTGATTAAAATGCCTAAAATAATTAAAAAACTATCTGTTAAAGATCTTATGGACCCAACTCCACTGACGAT
CTCTTCTGAACTGTCTGTATATGCTGCATTGAGCTTACTTGCAGATAATGAGTGTCGTAGCGCACCCGTTCTCGATGAAA
ATAAGCGTTTGCTTGGTTTTATCTCACAGCAAGATCTACTGCGTAGCCTTTGGTCTGAAGAGTTTGCCAGAGGGGTTTCA
TGTAAGGTTAGTGATTTGATGCAAACGGAGGTGATGACGCTGTCACCACAAGACTCAGTTGCTGAACTAATAGAGTTAAT
GGTCGTTGATCGTAGTAAGTTATTCCCGGTTAATGAATACGGGATCTTAACGGGCAATACTTTTAAAAGCTATGAAGAGC
GATTACGCCGTGCGTCGCCAAATAAGCCGAGTGTCTTTCCTGTTTTAGATAATGGCCTATTGTGTGGAGTGATCACACGA
GAAGCGATAGCCAAAAGGGTGTGTGAGGTTTATAGAGTCTAG

Upstream 100 bases:

>100_bases
AATTATATTTGTAGGTACAAAATAACAAGTTAGTAGATAAAAATGGGCAATAGAGTAATGAAAATGACAAATTTATACGC
TTCCATTTGGAGTGGAATAA

Downstream 100 bases:

>100_bases
TTTTTATTTTAATGATGTGAAACGGGACGATATCGTCCCGTTTTTCTTATCTTACTAGCTTTTCATCTGCCGAATACGGC
TTAATCTTTTTGCCTCTGTG

Product: formate/nitrite transporter

Products: formate [Periplasm]; formate [Cytoplasm] [C]

Alternate protein names: Formate channel 1 [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MFSNHAVKAPVIVNPIQQSVELPKPELLSLYQQAEHYGKAKVIKSAWQSFGLAAFAGAFIALAFVFYITVTTGSDGAWGL
VRLAGGLAFSLGLMLVVICGGELFTSTVLSSVAWAQKLVTTIDLIKCWGRVYLGNLIGAVIMLILIMSARMHELDGGLWG
LNALQIAQHKLHHSWGQAFVLGVLCNMLVCLGVWMTFSSRDALTKAILLMLPVAMFVSSGFEHSIANLFMVPLGITIHQF
ADPAWFEALNISRDSFADLTLSNFIFNNLIPVTLGNIVGGGVFVGLGYWLIEKSDQSLKTSLVHSTPQLETSAQIHQIPT
SGVLIKMPKIIKKLSVKDLMDPTPLTISSELSVYAALSLLADNECRSAPVLDENKRLLGFISQQDLLRSLWSEEFARGVS
CKVSDLMQTEVMTLSPQDSVAELIELMVVDRSKLFPVNEYGILTGNTFKSYEERLRRASPNKPSVFPVLDNGLLCGVITR
EAIAKRVCEVYRV

Sequences:

>Translated_493_residues
MFSNHAVKAPVIVNPIQQSVELPKPELLSLYQQAEHYGKAKVIKSAWQSFGLAAFAGAFIALAFVFYITVTTGSDGAWGL
VRLAGGLAFSLGLMLVVICGGELFTSTVLSSVAWAQKLVTTIDLIKCWGRVYLGNLIGAVIMLILIMSARMHELDGGLWG
LNALQIAQHKLHHSWGQAFVLGVLCNMLVCLGVWMTFSSRDALTKAILLMLPVAMFVSSGFEHSIANLFMVPLGITIHQF
ADPAWFEALNISRDSFADLTLSNFIFNNLIPVTLGNIVGGGVFVGLGYWLIEKSDQSLKTSLVHSTPQLETSAQIHQIPT
SGVLIKMPKIIKKLSVKDLMDPTPLTISSELSVYAALSLLADNECRSAPVLDENKRLLGFISQQDLLRSLWSEEFARGVS
CKVSDLMQTEVMTLSPQDSVAELIELMVVDRSKLFPVNEYGILTGNTFKSYEERLRRASPNKPSVFPVLDNGLLCGVITR
EAIAKRVCEVYRV
>Mature_493_residues
MFSNHAVKAPVIVNPIQQSVELPKPELLSLYQQAEHYGKAKVIKSAWQSFGLAAFAGAFIALAFVFYITVTTGSDGAWGL
VRLAGGLAFSLGLMLVVICGGELFTSTVLSSVAWAQKLVTTIDLIKCWGRVYLGNLIGAVIMLILIMSARMHELDGGLWG
LNALQIAQHKLHHSWGQAFVLGVLCNMLVCLGVWMTFSSRDALTKAILLMLPVAMFVSSGFEHSIANLFMVPLGITIHQF
ADPAWFEALNISRDSFADLTLSNFIFNNLIPVTLGNIVGGGVFVGLGYWLIEKSDQSLKTSLVHSTPQLETSAQIHQIPT
SGVLIKMPKIIKKLSVKDLMDPTPLTISSELSVYAALSLLADNECRSAPVLDENKRLLGFISQQDLLRSLWSEEFARGVS
CKVSDLMQTEVMTLSPQDSVAELIELMVVDRSKLFPVNEYGILTGNTFKSYEERLRRASPNKPSVFPVLDNGLLCGVITR
EAIAKRVCEVYRV

Specific function: Involved in the bidirectional transport of formate [H]

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family [H]

Homologues:

Organism=Escherichia coli, GI1787132, Length=264, Percent_Identity=50.7575757575758, Blast_Score=265, Evalue=6e-72,
Organism=Escherichia coli, GI1788837, Length=262, Percent_Identity=38.1679389312977, Blast_Score=194, Evalue=2e-50,
Organism=Escherichia coli, GI87082249, Length=248, Percent_Identity=31.8548387096774, Blast_Score=83, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6321779, Length=210, Percent_Identity=31.9047619047619, Blast_Score=100, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000292 [H]

Pfam domain/function: PF01226 Form_Nir_trans [H]

EC number: NA

Molecular weight: Translated: 53996; Mature: 53996

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS01005 FORMATE_NITRITE_TP_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate [Cytoplasm]; formate [Periplasm] [C]

Specific reaction: formate [Cytoplasm] = formate [Periplasm] formate [Periplasm] = formate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]