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The map label for this gene is cobB [H]

Identifier: 167624403

GI number: 167624403

Start: 2983949

End: 2984674

Strand: Reverse

Name: cobB [H]

Synonym: Shal_2481

Alternate gene names: 167624403

Gene position: 2984674-2983949 (Counterclockwise)

Preceding gene: 167624405

Following gene: 167624402

Centisome position: 57.1

GC content: 45.32

Gene sequence:

>726_bases
ATGTACCAGCAGATTGTAATATTGACCGGGGCAGGGATCTCAGCAGAATCAGGGTTACGTACCTTTAGAGATCAAGATGG
TTTATGGGAAGAACATAAAATAGAGGAGGTCGCAACTCCAGAGGGGTACGCCAGTAACCCCCTTTTAGTCGAGCGTTTCT
ATAATGATCGTTGGCAGCAATTCCATAATGGTTGTATCGAACCCAATGCTGGCCATACAGCCCTTGCTAAGTTAGAGCAA
CAATTTGAAGGCGAGCTGTTAGTCGTTACACAAAATATTGATGATCTACATGAGAGAGCGGGTTCGAAACGTCTTTTGCA
CATGCATGGTGAGCTATCCAAGGGGCGCTGCCCACGCTCACAGCAAACTTTTATATTAAGGGAACCCTTCGGGCCGAGGC
ATACTTGTACTTGCTGTATCCCTGCTCAGAGGTTGAGACCTCACGTTGTCTGGTTTGGCGAAATGCCATTTGGTTTAGAC
AGAATCCAGCATGCGCTTGATAGTTGTGATCTATTTATCGCTATAGGTACATCGGGCACGGTTTATCCAGCGGCAGGTTT
TGTTGATACGGCAAACCATCATGGTGCGCAAACGGTTGAGGTCAATCTTGTTGCAGTAGATAGACATAGTCAGTTTCAAT
ATCACCTAGAAGGTAAGGCGAGTGAGTTAGTACCAAAACTTGTCGATGATATTCTCAGTGGAAAAATAGTGAACAACGTA
CTTTAG

Upstream 100 bases:

>100_bases
AGTCATTTTGTATTGTTTTTGCCCATTTATACTAACGCCTAATGTCATATACGCCTACAAATAGGCAAAATAGTAACAAA
GACCGCAAAAAGGACTGTAG

Downstream 100 bases:

>100_bases
CATATGTGCTTAAGCGTATTATTAAGGTTCTCGCGATAACGCGAGTAAAGGGTAATAGTTGATGAAACAGCAAAAAGATG
TTGGTGGGATAGCGATCATT

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MYQQIVILTGAGISAESGLRTFRDQDGLWEEHKIEEVATPEGYASNPLLVERFYNDRWQQFHNGCIEPNAGHTALAKLEQ
QFEGELLVVTQNIDDLHERAGSKRLLHMHGELSKGRCPRSQQTFILREPFGPRHTCTCCIPAQRLRPHVVWFGEMPFGLD
RIQHALDSCDLFIAIGTSGTVYPAAGFVDTANHHGAQTVEVNLVAVDRHSQFQYHLEGKASELVPKLVDDILSGKIVNNV
L

Sequences:

>Translated_241_residues
MYQQIVILTGAGISAESGLRTFRDQDGLWEEHKIEEVATPEGYASNPLLVERFYNDRWQQFHNGCIEPNAGHTALAKLEQ
QFEGELLVVTQNIDDLHERAGSKRLLHMHGELSKGRCPRSQQTFILREPFGPRHTCTCCIPAQRLRPHVVWFGEMPFGLD
RIQHALDSCDLFIAIGTSGTVYPAAGFVDTANHHGAQTVEVNLVAVDRHSQFQYHLEGKASELVPKLVDDILSGKIVNNV
L
>Mature_241_residues
MYQQIVILTGAGISAESGLRTFRDQDGLWEEHKIEEVATPEGYASNPLLVERFYNDRWQQFHNGCIEPNAGHTALAKLEQ
QFEGELLVVTQNIDDLHERAGSKRLLHMHGELSKGRCPRSQQTFILREPFGPRHTCTCCIPAQRLRPHVVWFGEMPFGLD
RIQHALDSCDLFIAIGTSGTVYPAAGFVDTANHHGAQTVEVNLVAVDRHSQFQYHLEGKASELVPKLVDDILSGKIVNNV
L

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI300795542, Length=233, Percent_Identity=39.0557939914163, Blast_Score=155, Evalue=3e-38,
Organism=Homo sapiens, GI6912664, Length=254, Percent_Identity=37.007874015748, Blast_Score=150, Evalue=7e-37,
Organism=Homo sapiens, GI13787215, Length=242, Percent_Identity=37.603305785124, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI7657575, Length=248, Percent_Identity=27.8225806451613, Blast_Score=75, Evalue=5e-14,
Organism=Escherichia coli, GI308199517, Length=231, Percent_Identity=54.978354978355, Blast_Score=256, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI17541892, Length=197, Percent_Identity=30.9644670050761, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17137536, Length=229, Percent_Identity=27.0742358078603, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28571445, Length=118, Percent_Identity=34.7457627118644, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 27006; Mature: 27006

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQQIVILTGAGISAESGLRTFRDQDGLWEEHKIEEVATPEGYASNPLLVERFYNDRWQQ
CCCEEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
FHNGCIEPNAGHTALAKLEQQFEGELLVVTQNIDDLHERAGSKRLLHMHGELSKGRCPRS
HHCCCCCCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHCCCCCEEEEECCCCCCCCCCCC
QQTFILREPFGPRHTCTCCIPAQRLRPHVVWFGEMPFGLDRIQHALDSCDLFIAIGTSGT
CCEEEEECCCCCCCEEEEECCHHHCCCEEEEEECCCCCHHHHHHHHCCCCEEEEECCCCC
VYPAAGFVDTANHHGAQTVEVNLVAVDRHSQFQYHLEGKASELVPKLVDDILSGKIVNNV
EEECCCCEECCCCCCCEEEEEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHC
L
C
>Mature Secondary Structure
MYQQIVILTGAGISAESGLRTFRDQDGLWEEHKIEEVATPEGYASNPLLVERFYNDRWQQ
CCCEEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
FHNGCIEPNAGHTALAKLEQQFEGELLVVTQNIDDLHERAGSKRLLHMHGELSKGRCPRS
HHCCCCCCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHCCCCCEEEEECCCCCCCCCCCC
QQTFILREPFGPRHTCTCCIPAQRLRPHVVWFGEMPFGLDRIQHALDSCDLFIAIGTSGT
CCEEEEECCCCCCCEEEEECCHHHCCCEEEEEECCCCCHHHHHHHHCCCCEEEEECCCCC
VYPAAGFVDTANHHGAQTVEVNLVAVDRHSQFQYHLEGKASELVPKLVDDILSGKIVNNV
EEECCCCEECCCCCCCEEEEEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]