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The map label for this gene is rlmL

Identifier: 167624277

GI number: 167624277

Start: 2842717

End: 2844852

Strand: Reverse

Name: rlmL

Synonym: Shal_2353

Alternate gene names: 167624277

Gene position: 2844852-2842717 (Counterclockwise)

Preceding gene: 167624279

Following gene: 167624276

Centisome position: 54.43

GC content: 42.84

Gene sequence:

>2136_bases
ATGTTAAATTTTTTTGCTGCAGCTCCACGGGGCTATGAATACGCGTTATCACTTGAGCTCGCAGAGCTTGGCGCTTCTGA
AATAAAAGAAAGTGTTGCGGGAGTTTATTTTTCTGCTCCGTTAGAGCTGGGATATCGTATTACCCTATGGTCGCGTTTGG
CGAGCAGAATCATTTTTGTCATTTATAAAGGTCCATGTGAGTCACCTGAGCAGCTGTATAACGCGGCTTACGGTATTGAC
TGGCAGATGCAGTTTTCAAATCGCAGTACTTTTAGCATCGACTTCCATGGTTTGGGTGGTTTTATTAAGAACACCCAGTT
TGGTGCACTAAAAATCAAAGATGCGATTGTAGATCGTTTTCGTGATGATGATTTTTCTCGTCCAAATGTTGAAAGAGTCG
ATGCCGATTTTAGAATTGATGCCCACTTTAGACGTGGTGAGATCACTATCGGTTTCAATTTCTCAGGCCCAGCGCTGCAC
AAGCGTGGCTATCGCTCGACAACGGGTGAGGCACCACTAAAAGAAAACCTAGCTGCTAATATGCTAGTTCGTAGTGGTTG
GCAAGCTGCACAAGTAGACTTATTGGATCCATTCTGTGGTAGTGGAACCATTTTGATTGAAGCGGCGATGATGGCGTGTG
ATATTGCACCAGGTATTCATCGTGAGCGTTTTGGTTTTGACCATTGGTTGCCTCATAACAAGAAAGTTTGGGAAGAGTTA
TTAAACGAAGCCCGTGCGCGTGCGTCTATCGGTAAGACTCGTTGTGAGATTAAATTCTATGGCTCAGATATTGACTCTAG
ACTGGTAGCATTAGCGAAACGCAATGCCGAGAATGCTGGTGTGCTAGAACTTATCGATTTTAACGTCTGCAATGCGTTAG
ACGTTAAAGCGCCAGCAGGATCAGGATATTTAATATCTAATCCGCCGTACGGTGAGCGTTTAGGTAACGTCACCTCATTA
TTGCAGCTTTATTACCAACTAGGTGATAAGTTCAAGGCCGATTTCGGCGGCTGGAGCATCGCTATCTTAAACAGTGATGT
TGAGCTGTTATCTGCATTAAAGCTCAAAGCTGACAAGCAGATGAAGATGAATAACGGCGCTTTAGAGTGTGCGTTTAACC
TTTATACAGTTCATGCGCTGAATACGCGCCGTGTCGATCCTGCTAACATCAAAATTGAAGGTGATGTCAGTGATATCGCC
GTACCTTTTGCTAATCGTATTAAAAAGAACTTTAAGCAGCTAGAGAAATGGGCTAAGAAAGAGGGCATCGATAGTTACCG
TTTATATGATGCAGATATACCTGAATATAACGTAGCGATTGATAAGTACCTCGATTATGTCGTGATCCAAGAGTATTCAG
CACCATCAGAAATCCCTGAAGCGGTAACTAAACGCAGATTAACCGACGTATTGATCTCACTACCTAGTGCGATTGGCATC
GACCCTAATCACATCATTTTGAAAACCCGTGAGCGTAAGAAGGGTACTAATCAATATGAAAAGTTAGCCGCGAGTAAGCT
TGAGCTGATCACAACAGAATATGGCGCTAAATTTAAGTTAAACCTTAAAGAGTACCTAGATACTGGCCTATTTTTAGATC
ATCGATTAACTCGTAAGTTAGTGGGTGAAAAATCTAAAGGTCGCAGTGTATGCAACCTGTTTTCATATACCGGGTCGGCA
TCTGTACATGCAGCTTTAGGTGGGGCGACTTCGGTCACTACCGTAGACATGTCTAATACTTATATCGATTGGGCAAAAGA
AAACTTTGCATTAAATGGCTTAAATAGCGATAAATATCAGTTTGTTCAGGCTAACTGCCTGCAATGGATGAAGCGCACAC
ACGATAGGTTTGACCTGATTTTTATCGATCCTCCGACCTTCTCAAACTCGAAGCGGATGGAAGACTCATTTGACGTGCAG
CGTGATCACCTTGCCATGTTAACTGACGTGTTCAAACTGCTAAATCCTGGTGGGGAAATTATTTTCTCCAACAATAAGCG
CAAATTTAAGATGGAAATCGCTGAGCTTGAAGCACTAGGCATGTCAGTTAAAAACATCGATAACCAAACATTACCTTTGG
ATTACAAGCGCAACCCGCATATCCACAATACATGGTTAATCACCCATGCAGGCTAA

Upstream 100 bases:

>100_bases
GTTACGGTTATTAAATCGGGCTTTAGCCAATAACTTTTATCATTTAGTAGCACGAACAGGTATAATGCCTAACTAATTTT
TACTGTGTGTCGACTAATCA

Downstream 100 bases:

>100_bases
CGCCTCGGTTATTTTATATCACACAGATGCGTGCCATCTGTGTGAATTAGCAGCTGATTTACTCATGCAGGCAGGCATTA
CCTTTAATGCCTTGGATATC

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL

Number of amino acids: Translated: 711; Mature: 711

Protein sequence:

>711_residues
MLNFFAAAPRGYEYALSLELAELGASEIKESVAGVYFSAPLELGYRITLWSRLASRIIFVIYKGPCESPEQLYNAAYGID
WQMQFSNRSTFSIDFHGLGGFIKNTQFGALKIKDAIVDRFRDDDFSRPNVERVDADFRIDAHFRRGEITIGFNFSGPALH
KRGYRSTTGEAPLKENLAANMLVRSGWQAAQVDLLDPFCGSGTILIEAAMMACDIAPGIHRERFGFDHWLPHNKKVWEEL
LNEARARASIGKTRCEIKFYGSDIDSRLVALAKRNAENAGVLELIDFNVCNALDVKAPAGSGYLISNPPYGERLGNVTSL
LQLYYQLGDKFKADFGGWSIAILNSDVELLSALKLKADKQMKMNNGALECAFNLYTVHALNTRRVDPANIKIEGDVSDIA
VPFANRIKKNFKQLEKWAKKEGIDSYRLYDADIPEYNVAIDKYLDYVVIQEYSAPSEIPEAVTKRRLTDVLISLPSAIGI
DPNHIILKTRERKKGTNQYEKLAASKLELITTEYGAKFKLNLKEYLDTGLFLDHRLTRKLVGEKSKGRSVCNLFSYTGSA
SVHAALGGATSVTTVDMSNTYIDWAKENFALNGLNSDKYQFVQANCLQWMKRTHDRFDLIFIDPPTFSNSKRMEDSFDVQ
RDHLAMLTDVFKLLNPGGEIIFSNNKRKFKMEIAELEALGMSVKNIDNQTLPLDYKRNPHIHNTWLITHAG

Sequences:

>Translated_711_residues
MLNFFAAAPRGYEYALSLELAELGASEIKESVAGVYFSAPLELGYRITLWSRLASRIIFVIYKGPCESPEQLYNAAYGID
WQMQFSNRSTFSIDFHGLGGFIKNTQFGALKIKDAIVDRFRDDDFSRPNVERVDADFRIDAHFRRGEITIGFNFSGPALH
KRGYRSTTGEAPLKENLAANMLVRSGWQAAQVDLLDPFCGSGTILIEAAMMACDIAPGIHRERFGFDHWLPHNKKVWEEL
LNEARARASIGKTRCEIKFYGSDIDSRLVALAKRNAENAGVLELIDFNVCNALDVKAPAGSGYLISNPPYGERLGNVTSL
LQLYYQLGDKFKADFGGWSIAILNSDVELLSALKLKADKQMKMNNGALECAFNLYTVHALNTRRVDPANIKIEGDVSDIA
VPFANRIKKNFKQLEKWAKKEGIDSYRLYDADIPEYNVAIDKYLDYVVIQEYSAPSEIPEAVTKRRLTDVLISLPSAIGI
DPNHIILKTRERKKGTNQYEKLAASKLELITTEYGAKFKLNLKEYLDTGLFLDHRLTRKLVGEKSKGRSVCNLFSYTGSA
SVHAALGGATSVTTVDMSNTYIDWAKENFALNGLNSDKYQFVQANCLQWMKRTHDRFDLIFIDPPTFSNSKRMEDSFDVQ
RDHLAMLTDVFKLLNPGGEIIFSNNKRKFKMEIAELEALGMSVKNIDNQTLPLDYKRNPHIHNTWLITHAG
>Mature_711_residues
MLNFFAAAPRGYEYALSLELAELGASEIKESVAGVYFSAPLELGYRITLWSRLASRIIFVIYKGPCESPEQLYNAAYGID
WQMQFSNRSTFSIDFHGLGGFIKNTQFGALKIKDAIVDRFRDDDFSRPNVERVDADFRIDAHFRRGEITIGFNFSGPALH
KRGYRSTTGEAPLKENLAANMLVRSGWQAAQVDLLDPFCGSGTILIEAAMMACDIAPGIHRERFGFDHWLPHNKKVWEEL
LNEARARASIGKTRCEIKFYGSDIDSRLVALAKRNAENAGVLELIDFNVCNALDVKAPAGSGYLISNPPYGERLGNVTSL
LQLYYQLGDKFKADFGGWSIAILNSDVELLSALKLKADKQMKMNNGALECAFNLYTVHALNTRRVDPANIKIEGDVSDIA
VPFANRIKKNFKQLEKWAKKEGIDSYRLYDADIPEYNVAIDKYLDYVVIQEYSAPSEIPEAVTKRRLTDVLISLPSAIGI
DPNHIILKTRERKKGTNQYEKLAASKLELITTEYGAKFKLNLKEYLDTGLFLDHRLTRKLVGEKSKGRSVCNLFSYTGSA
SVHAALGGATSVTTVDMSNTYIDWAKENFALNGLNSDKYQFVQANCLQWMKRTHDRFDLIFIDPPTFSNSKRMEDSFDVQ
RDHLAMLTDVFKLLNPGGEIIFSNNKRKFKMEIAELEALGMSVKNIDNQTLPLDYKRNPHIHNTWLITHAG

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1787181, Length=713, Percent_Identity=48.5273492286115, Blast_Score=686, Evalue=0.0,
Organism=Escherichia coli, GI145693111, Length=264, Percent_Identity=29.5454545454545, Blast_Score=105, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLML_SHEHH (B0TIX7)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001674571.1
- ProteinModelPortal:   B0TIX7
- SMR:   B0TIX7
- GeneID:   5902817
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_2353
- HOGENOM:   HBG289536
- OMA:   YRIYDAD
- ProtClustDB:   PRK11783
- BioCyc:   SHAL458817:SHAL_2353-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01858
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114
- PIRSF:   PIRSF037618
- SMART:   SM00981

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020

EC number: =2.1.1.173

Molecular weight: Translated: 80093; Mature: 80093

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: PS51165 THUMP; PS01261 UPF0020; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNFFAAAPRGYEYALSLELAELGASEIKESVAGVYFSAPLELGYRITLWSRLASRIIFV
CCCCEECCCCCCEEEEEEEHHHCCHHHHHHHHCCEEEECCHHCCEEEEHHHHHHCCEEEE
IYKGPCESPEQLYNAAYGIDWQMQFSNRSTFSIDFHGLGGFIKNTQFGALKIKDAIVDRF
EECCCCCCHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCHHCCCCCCEEEEHHHHHHHH
RDDDFSRPNVERVDADFRIDAHFRRGEITIGFNFSGPALHKRGYRSTTGEAPLKENLAAN
CCCCCCCCCHHHCCCCEEEEEEEECCEEEEEEECCCCHHHCCCCCCCCCCCCHHHHHHHH
MLVRSGWQAAQVDLLDPFCGSGTILIEAAMMACDIAPGIHRERFGFDHWLPHNKKVWEEL
HHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCHHHHHHH
LNEARARASIGKTRCEIKFYGSDIDSRLVALAKRNAENAGVLELIDFNVCNALDVKAPAG
HHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCEEEEEECCCCCEEECCCCCC
SGYLISNPPYGERLGNVTSLLQLYYQLGDKFKADFGGWSIAILNSDVELLSALKLKADKQ
CCEEECCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCEEEEEECCCHHHHHHHHHCCCCC
MKMNNGALECAFNLYTVHALNTRRVDPANIKIEGDVSDIAVPFANRIKKNFKQLEKWAKK
EECCCCCEEEEEEEEEEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
EGIDSYRLYDADIPEYNVAIDKYLDYVVIQEYSAPSEIPEAVTKRRLTDVLISLPSAIGI
CCCCCEEEEECCCCCCHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHCCC
DPNHIILKTRERKKGTNQYEKLAASKLELITTEYGAKFKLNLKEYLDTGLFLDHRLTRKL
CCCEEEEEECHHCCCCCHHHHHHHHHHHEEEECCCCEEEEEHHHHHCCCCHHHHHHHHHH
VGEKSKGRSVCNLFSYTGSASVHAALGGATSVTTVDMSNTYIDWAKENFALNGLNSDKYQ
HCCCCCCCHHHHHHHCCCCCEEEEECCCCCEEEEEECCCCEEEECCCCEEEECCCCCHHH
FVQANCLQWMKRTHDRFDLIFIDPPTFSNSKRMEDSFDVQRDHLAMLTDVFKLLNPGGEI
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCE
IFSNNKRKFKMEIAELEALGMSVKNIDNQTLPLDYKRNPHIHNTWLITHAG
EECCCCEEEEEHHHHHHHHCCHHHCCCCCCCCCCCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MLNFFAAAPRGYEYALSLELAELGASEIKESVAGVYFSAPLELGYRITLWSRLASRIIFV
CCCCEECCCCCCEEEEEEEHHHCCHHHHHHHHCCEEEECCHHCCEEEEHHHHHHCCEEEE
IYKGPCESPEQLYNAAYGIDWQMQFSNRSTFSIDFHGLGGFIKNTQFGALKIKDAIVDRF
EECCCCCCHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCHHCCCCCCEEEEHHHHHHHH
RDDDFSRPNVERVDADFRIDAHFRRGEITIGFNFSGPALHKRGYRSTTGEAPLKENLAAN
CCCCCCCCCHHHCCCCEEEEEEEECCEEEEEEECCCCHHHCCCCCCCCCCCCHHHHHHHH
MLVRSGWQAAQVDLLDPFCGSGTILIEAAMMACDIAPGIHRERFGFDHWLPHNKKVWEEL
HHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCHHHHHHH
LNEARARASIGKTRCEIKFYGSDIDSRLVALAKRNAENAGVLELIDFNVCNALDVKAPAG
HHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCEEEEEECCCCCEEECCCCCC
SGYLISNPPYGERLGNVTSLLQLYYQLGDKFKADFGGWSIAILNSDVELLSALKLKADKQ
CCEEECCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCEEEEEECCCHHHHHHHHHCCCCC
MKMNNGALECAFNLYTVHALNTRRVDPANIKIEGDVSDIAVPFANRIKKNFKQLEKWAKK
EECCCCCEEEEEEEEEEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
EGIDSYRLYDADIPEYNVAIDKYLDYVVIQEYSAPSEIPEAVTKRRLTDVLISLPSAIGI
CCCCCEEEEECCCCCCHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHCCC
DPNHIILKTRERKKGTNQYEKLAASKLELITTEYGAKFKLNLKEYLDTGLFLDHRLTRKL
CCCEEEEEECHHCCCCCHHHHHHHHHHHEEEECCCCEEEEEHHHHHCCCCHHHHHHHHHH
VGEKSKGRSVCNLFSYTGSASVHAALGGATSVTTVDMSNTYIDWAKENFALNGLNSDKYQ
HCCCCCCCHHHHHHHCCCCCEEEEECCCCCEEEEEECCCCEEEECCCCEEEECCCCCHHH
FVQANCLQWMKRTHDRFDLIFIDPPTFSNSKRMEDSFDVQRDHLAMLTDVFKLLNPGGEI
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCE
IFSNNKRKFKMEIAELEALGMSVKNIDNQTLPLDYKRNPHIHNTWLITHAG
EECCCCEEEEEHHHHHHHHCCHHHCCCCCCCCCCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA